ViewGene
Locus Name:
GeneID:
Location:

GeneID:4230
TrivialName: PA14_66770
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66770
PAO1 Ortholog Locus:PA5053
Sequence Length:534
Protein Length:177
Start: 5963960
Stop: 5964493
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08438.1|AE004918_8 (AE004918) heat shock protein HslV [Pseudomonas aeruginosa PAO1]
Identities = 177/177 (100%), Positives = 177/177 (100%)
Sequence:
TTGACCACCATCGTATCTGTCCGCCGCAACGGCAAAGTCGTCATGGGCGGCGACGGCCAGGTTTCCCTGGGC
AACACCGTGATGAAAGGCAACGCCAAGAAGGTCCGGCGCCTGTATCACGGTCAGGTACTGGCCGGTTTCGCC
GGCGCCACCGCCGATGCCTTCACCCTGTTCGAGCGCTTCGAGCAGCAACTGGAGAAACACCAGGGGCACCTG
GTCCGCGCCGCCGTCGAACTGGCCAAGGACTGGCGCACCGACCGCTCCCTGAGCCGCCTCGAAGCCATGCTC
GCGGTCGCCAACAAGGATGCCTCGCTGATCATCACCGGCAACGGCGACGTGGTCGAACCCGAACACGGCCTG
ATCGCCATGGGCTCCGGCGGCGGCTTCGCCCAGGCCGCGGCCCTGGCCCTGCTGCAGCACAACGCCGAGCTG
AGCGCTCGGGAAGTCGCCGAGACCGCGCTGAACATCGCCGGCTCGATCTGCGTCTTCACCAACCAGAACCTG
ACCATCGAGGAGCTGGACAGCGCCGTCTGA
Translation:
MTTIVSVRRNGKVVMGGDGQVSLGNTVMKGNAKKVRRLYHGQVLAGFAGATADAFTLFERFEQQLEKHQGHL
VRAAVELAKDWRTDRSLSRLEAMLAVANKDASLIITGNGDVVEPEHGLIAMGSGGGFAQAAALALLQHNAEL
SAREVAETALNIAGSICVFTNQNLTIEELDSAV*
AnnotationID:4236GeneID:4230
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 11:11:15
Gene Name:hslV
Confidence Code:2
GeneProduct:heat shock protein HslV
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG5405
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:3.4.25.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70566.1|AE016699_7 (AE016792) heat shock protein HslV [Pseudomonas putida KT2440]
          Length = 176

 Score =  304 bits (778), Expect = 2e-82
 Identities = 159/176 (90%), Positives = 165/176 (93%), Gaps = 1/176 (0%)

>gb|AAO58567.1| (AE016874) heat shock protein HslV [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 176

 Score =  296 bits (759), Expect = 3e-80
 Identities = 155/176 (88%), Positives = 163/176 (92%), Gaps = 1/176 (0%)

>gb|AAN57135.1|AE015849_2 (AE015849) ATP-dependent protease HslV [Shewanella oneidensis MR-1]
          Length = 174

 Score =  268 bits (684), Expect = 1e-71
 Identities = 138/174 (79%), Positives = 154/174 (88%), Gaps = 1/174 (0%)






 
Structural Features:
COG5405, HslV, ATP-dependent protease HslVU (ClpYQ), peptidase subunit 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 178 residues,  98.3% aligned
Score =  270 bits (692), Expect = 6e-74
Identities = 123/176 (69%), Positives = 151/176 (85%), Gaps = 1/176 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:15046




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25603




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35015




  
Author/Investigator(s):
        





  
Title:
        CD




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4230_cdd.html


    		




 
URL:

















    ReferenceID:35833




  
Author/Investigator(s):
        Chuang SE, Burland V, Plunkett G 3rd, Daniels DL, Blattner FR.





  
Title:
        Sequence analysis of four new heat-shock genes constituting the hslTS/ibpAB and hslVU operons in Escherichia coli.




  
PubMed:
        8244018


MedLine:
        




    
Source:
Gene. 1993 Nov 30;134(1):1-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=94063501&dopt=Abstract

















    ReferenceID:35835




  
Author/Investigator(s):
        Lin M, Li Y.





  
Title:
        PCR genome walking identifies a genetic locus comprising two heat shock genes (hslV and hslU) from Leptospira borgpetersenii serovar hardjobovis.




  
PubMed:
        11685516


MedLine:
        




    
Source:
Curr Microbiol. 2001 Dec;43(6):452-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11685516









           
Homologs By Global Alignment 

           

       



     
Gene ID:4230
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6520
                           PA5053_tr
                           translation of PA5053
                           177
                           99.99
                           99.99
                           0
                           877.0
     

     
     
177132
                           gb|AAG08438.1|AE004918_8
                           (AE004918) heat shock protein HslV [Pseudomonas aeruginosa PAO1]
                           177
                           99.43
                           99.99
                           0
                           875.0
     

     
     
177133
                           gb|AAN70566.1|AE016699_7
                           (AE016792) heat shock protein HslV [Pseudomonas putida KT2440]
                           177
                           89.26
                           93.22
                           1
                           774.0
     

     
     
177134
                           gb|AAO58567.1|
                           (AE016874) heat shock protein HslV [Pseudomonas syringae pv. tomato str. DC3000]
                           177
                           87.00
                           92.09
                           1
                           755.0
     

     
     
177135
                           gb|AAN57135.1|AE015849_2
                           (AE015849) ATP-dependent protease HslV [Shewanella oneidensis MR-1]
                           177
                           77.40
                           87.00
                           3
                           681.0
     

     






      

          
GC ORFID: 47232How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5053Glimmer Score: 

          
Start: 5227643Stop: 5228176

          
Length: 534


          
Start Codon: TTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5053 translation of PA5053Homolog Bit Score 342.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3789 (ORF: BLASTX 300.0), t_PA5053 (ORF: GLIMMER)

          
GC ORF Sequence

           
TTGACCACCATCGTATCTGTCCGCCGCAACGGCAAAGTCGTCATGGGCGGCGACGGCCAGGTTTCCCTGGGC 
AACACCGTGATGAAAGGCAACGCCAAGAAGGTCCGGCGCCTGTATCACGGTCAGGTACTGGCCGGTTTCGCC 
GGCGCCACCGCCGATGCCTTCACCCTGTTCGAGCGCTTCGAGCAGCAACTGGAGAAACACCAGGGGCACCTG 
GTCCGCGCCGCCGTCGAACTGGCCAAGGACTGGCGCACCGACCGCTCCCTGAGCCGCCTCGAAGCCATGCTC 
GCGGTCGCCAACAAGGATGCCTCGCTGATCATCACCGGCAACGGCGACGTGGTCGAACCCGAACACGGCCTG 
ATCGCCATGGGCTCCGGCGGCGGCTTCGCCCAGGCCGCGGCCCTGGCCCTGCTGCAGCACAACGCCGAGCTG 
AGCGCTCGGGAAGTCGCCGAGACCGCGCTGAACATCGCCGGCTCGATCTGCGTCTTCACCAACCAGAACCTG 
ACCATCGAGGAGCTGGACAGCGCCGTCTGA