ViewGene
Locus Name:
GeneID:
Location:

GeneID:423
TrivialName: PA14_65660
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65660
PAO1 Ortholog Locus:PA4967
Sequence Length:1890
Protein Length:629
Start: 5849307
Stop: 5847418
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08352.1|AE004909_8 (AE004909) topoisomerase IV subunit B [Pseudomonas aeruginosa PAO1]
Identities = 628/629 (99%), Positives = 629/629 (100%)
Sequence:
ATGGCTACTTACAACGCAGACGCCATCGAAGTCCTTTCCGGCCTCGACCCGGTGCGCAAGCGCCCGGGGATG
TACACCGACACCACCCGCCCCAACCACCTGGCCCAGGAAGTCATCGACAACAGCGTCGACGAAGCCCTGGCC
GGCCACGCGAAGAGCGTGCAGGTGATCCTGCACCAGGACAACTCGCTGGAAGTCATCGACGACGGCCGCGGC
ATGCCGGTGGACATCCACCCGGAAGAGGGCGTGCCGGGCGTCGAGCTGATCCTTACCAAGCTGCATGCCGGC
GGCAAGTTCTCGAACAAGAACTACCAGTTCTCCGGCGGCTTGCACGGGGTCGGCATCTCGGTGGTGAACGCG
CTCTCGACCCGGGTCGAGGTACGCGTCAAGCGCGACGCCAACGAGTACCGGATGACCTTCGCCGACGGCTTC
AAGGACAGCGATCTGGAAGTCATCGGCACGGTCGGCAAGCGCAATACCGGTACCAGCGTGCATTTCTGGCCG
GATCCGAAGTATTTCGATTCGGCGAAGTTCTCGGTCAGCCGCCTCAAGCATGTGCTCAAGGCCAAGGCGGTG
CTGTGCCCGGGCCTGAGCGTGGTGTTCGAGGACAAGAACACCGGCGAGCGCGTCGAGTGGCACTTCGAGGAC
GGCCTGCGCTCCTACCTGACCGACGCGGTCGCCGAGCTGCCGCGCCTGCCCGATGAACCCTTCTGCGGCAAC
CTCGAAGGTTCCAAGGAAGCGGTGAGCTGGGCCCTGCTGTGGCTGCCCGAGGGCGGTGAGTCGGTGCAGGAA
AGCTACGTCAACCTGATTCCCACGGCCCAGGGCGGCACCCATGTGAACGGCCTGCGCCAGGGCCTGCTCGAC
GCCATGCGCGAATTCTGCGAGTTCCGCAACCTGTTGCCGCGCGGAGTCAAGCTGGCGCCCGAGGACGTCTGG
GAACGGATCGCCTTCGTCCTCTCGATGAAGATGCAGGAACCGCAGTTCTCCGGGCAGACCAAGGAGCGCCTG
TCGTCCCGCGAGGCGGCGGCGTTCGTCTCGGGCGTGGTGAAGGACGCCTTCAGCCTGTGGCTCAACGAGCAC
GCCGAAATCGGCCTGCAACTGGCGGAACTGGCGATCAGCAACGCCGGGCGTCGCCTCAAGGCGGGCAAGAAG
GTCGAGCGCAAGAAGATCACCCAGGGGCCGGCGCTGCCCGGCAAACTGGCCGACTGCGCCGGACAGGAACCG
ATGCGCGCGGAACTGTTCCTGGTCGAGGGCGACTCCGCCGGCGGCTCGGCGAAGCAGGCGCGGGACAAGGAA
TTCCAGGCGATCATGCCGCTGCGCGGAAAGATCCTGAACACCTGGGAAGTGGACGGCGGCGAGGTGCTTGCC
AGCCAGGAGGTCCACGACATCGCGGTAGCCATCGGCGTCGATCCGGGCGCCAGCGACCTGGCTCAGCTGCGC
TACGGCAAGATCTGTATCCTCGCGGATGCCGACTCCGACGGGCTGCACATCGCCACGCTGCTCTGCGCGCTG
TTCGTCCGCCATTTCCGCCCGCTGGTGGAAGCCGGCCACGTCTACGTGGCGATGCCGCCGCTGTACCGCATC
GACCTCGGCAAGGAAATCTACTACGCCCTCGACGAAGCCGAGCGCGACGGCATCCTCGAGCGCCTGGCCGCC
GAGAAGAAGCGCGGCAAGCCGCAGGTCACCCGCTTCAAGGGCCTTGGCGAAATGAATCCGTTGCAACTGCGC
GAGACCACCATGGATCCGAATACCCGGCGGCTGGTCCAGCTCACCCTGGAGGACGCCACCGGTACCCTGGAG
ATCATGGACATGCTGCTGGCCAAGAAGCGCGCCGGTGACCGCAAGTCCTGGCTGGAGAGCAAGGGCAACCTG
GCCGAGGTGCTGGTCTGA
Translation:
MATYNADAIEVLSGLDPVRKRPGMYTDTTRPNHLAQEVIDNSVDEALAGHAKSVQVILHQDNSLEVIDDGRG
MPVDIHPEEGVPGVELILTKLHAGGKFSNKNYQFSGGLHGVGISVVNALSTRVEVRVKRDANEYRMTFADGF
KDSDLEVIGTVGKRNTGTSVHFWPDPKYFDSAKFSVSRLKHVLKAKAVLCPGLSVVFEDKNTGERVEWHFED
GLRSYLTDAVAELPRLPDEPFCGNLEGSKEAVSWALLWLPEGGESVQESYVNLIPTAQGGTHVNGLRQGLLD
AMREFCEFRNLLPRGVKLAPEDVWERIAFVLSMKMQEPQFSGQTKERLSSREAAAFVSGVVKDAFSLWLNEH
AEIGLQLAELAISNAGRRLKAGKKVERKKITQGPALPGKLADCAGQEPMRAELFLVEGDSAGGSAKQARDKE
FQAIMPLRGKILNTWEVDGGEVLASQEVHDIAVAIGVDPGASDLAQLRYGKICILADADSDGLHIATLLCAL
FVRHFRPLVEAGHVYVAMPPLYRIDLGKEIYYALDEAERDGILERLAAEKKRGKPQVTRFKGLGEMNPLQLR
ETTMDPNTRRLVQLTLEDATGTLEIMDMLLAKKRAGDRKSWLESKGNLAEVLV*
AnnotationID:432GeneID:423
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 08:08:48
Gene Name:parE
Confidence Code:1
GeneProduct:topoisomerase IV subunit B
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0187,smart00433
Secondary Functional Category(ies):
EC Number:5.99.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70482.1|AE016689_10 (AE016792) DNA topoisomerase IV, B subunit [Pseudomonas putida
           KT2440]
          Length = 634

 Score = 1140 bits (2950), Expect = 0.0
 Identities = 565/628 (89%), Positives = 598/628 (95%)

>gb|AAO58391.1| (AE016874) DNA topoisomerase IV, B subunit [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 634

 Score = 1138 bits (2944), Expect = 0.0
 Identities = 563/627 (89%), Positives = 599/627 (95%)

>gb|AAN56874.1|AE015822_6 (AE015822) DNA topoisomerase IV, B subunit [Shewanella oneidensis
           MR-1]
          Length = 628

 Score =  967 bits (2501), Expect = 0.0
 Identities = 459/622 (73%), Positives = 543/622 (87%)








 
Structural Features:
COG0187, GyrB, Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), 
B subunit 
[DNA replication, recombination, and repair]
CD-Length = 635 residues,  99.4% aligned
Score =  863 bits (2232), Expect = 0.0
Identities = 304/637 (47%), Positives = 404/637 (63%), Gaps = 15/637 (2%)

smart00433, TOP2c, TopoisomeraseII; Eukaryotic DNA topoisomerase II, GyrB, ParE
CD-Length = 594 residues,  99.0% aligned
Score =  581 bits (1500), Expect = 7e-167
Identities = 239/594 (40%), Positives = 341/594 (57%), Gaps = 12/594 (2%)








   
Genomic Context:






   
Comment:


















    ReferenceID:849




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17991




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35073




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:423_cdd.html


    		




 
URL:

















    ReferenceID:35885




  
Author/Investigator(s):
        Akasaka T, Onodera Y, Tanaka M, Sato K.





  
Title:
        Cloning, expression, and enzymatic characterization of Pseudomonas aeruginosa topoisomerase IV.




  
PubMed:
        10049263


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1999 Mar;43(3):530-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:Antimicrob Agents Chemother. 1999 Mar;43(3):530-6.









           
Homologs By Global Alignment 

           

       



     
Gene ID:423
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
713
                           PA4967_tr
                           translation of PA4967
                           629
                           99.84
                           99.99
                           0
                           3238.0
     

     
     
32031
                           gb|AAG08352.1|AE004909_8
                           (AE004909) topoisomerase IV subunit B [Pseudomonas aeruginosa PAO1]
                           629
                           99.84
                           99.99
                           0
                           3238.0
     

     
     
32032
                           dbj|BAA37153.1|
                           (AB003429) topoisomerase IV subunit [Pseudomonas aeruginosa]
                           629
                           99.52
                           99.68
                           0
                           3227.0
     

     
     
32033
                           gb|AAN70482.1|AE016689_10
                           (AE016792) DNA topoisomerase IV, B subunit [Pseudomonas putida KT2440]
                           634
                           89.11
                           94.32
                           5
                           2949.0
     

     
     
32034
                           gb|AAO58391.1|
                           (AE016874) DNA topoisomerase IV, B subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           634
                           88.80
                           94.47
                           5
                           2943.0
     

     






      

          
GC ORFID: 43425How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4967Glimmer Score: 

          
Start: 5112989Stop: 5111100

          
Length: 1890


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4967 translation of PA4967Homolog Bit Score 1251.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3411 (ORF: BLASTX 1138.0), t_PA4967 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTACTTACAACGCAGACGCCATCGAAGTCCTTTCCGGCCTCGACCCGGTGCGCAAGCGCCCGGGGATG 
TACACCGACACCACCCGCCCCAACCACCTGGCCCAGGAAGTCATCGACAACAGCGTCGACGAAGCCCTGGCC 
GGCCACGCGAAGAGCGTGCAGGTGATCCTGCACCAGGACAACTCGCTGGAAGTCATCGACGACGGCCGCGGC 
ATGCCGGTGGACATCCACCCGGAAGAGGGCGTGCCGGGCGTCGAGCTGATCCTTACCAAGCTGCATGCCGGC 
GGCAAGTTCTCGAACAAGAACTACCAGTTCTCCGGCGGCTTGCACGGGGTCGGCATCTCGGTGGTGAACGCG 
CTCTCGACCCGGGTCGAGGTACGCGTCAAGCGCGACGCCAACGAGTACCGGATGACCTTCGCCGACGGCTTC 
AAGGACAGCGATCTGGAAGTCATCGGCACGGTCGGCAAGCGCAATACCGGTACCAGCGTGCATTTCTGGCCG 
GATCCGAAGTATTTCGATTCGGCGAAGTTCTCGGTCAGCCGCCTCAAGCATGTGCTCAAGGCCAAGGCGGTG 
CTGTGCCCGGGCCTGAGCGTGGTGTTCGAGGACAAGAACACCGGCGAGCGCGTCGAGTGGCACTTCGAGGAC 
GGCCTGCGCTCCTACCTGACCGACGCGGTCGCCGAGCTGCCGCGCCTGCCCGATGAACCCTTCTGCGGCAAC 
CTCGAAGGTTCCAAGGAAGCGGTGAGCTGGGCCCTGCTGTGGCTGCCCGAGGGCGGTGAGTCGGTGCAGGAA 
AGCTACGTCAACCTGATTCCCACGGCCCAGGGCGGCACCCATGTGAACGGCCTGCGCCAGGGCCTGCTCGAC 
GCCATGCGCGAATTCTGCGAGTTCCGCAACCTGTTGCCGCGCGGAGTCAAGCTGGCGCCCGAGGACGTCTGG 
GAACGGATCGCCTTCGTCCTCTCGATGAAGATGCAGGAACCGCAGTTCTCCGGGCAGACCAAGGAGCGCCTG 
TCGTCCCGCGAGGCGGCGGCGTTCGTCTCGGGCGTGGTGAAGGACGCCTTCAGCCTGTGGCTCAACGAGCAC 
GCCGAAATCGGCCTGCAACTGGCGGAACTGGCGATCAGCAACGCCGGGCGTCGCCTCAAGGCGGGCAAGAAG 
GTCGAGCGCAAGAAGATCACCCAGGGGCCGGCGCTGCCCGGCAAACTGGCCGACTGCGCCGGACAGGAACCG 
ATGCGCGCGGAACTGTTCCTGGTCGAGGGCGACTCCGCCGGCGGCTCGGCGAAGCAGGCGCGGGACAAGGAA 
TTCCAGGCGATCATGCCGCTGCGCGGAAAGATCCTGAACACCTGGGAAGTGGACGGCGGCGAGGTGCTTGCC 
AGCCAGGAGGTCCACGACATCGCGGTAGCCATCGGCGTCGATCCGGGCGCCAGCGACCTGGCTCAGCTGCGC 
TACGGCAAGATCTGTATCCTCGCGGATGCCGACTCCGACGGGCTGCACATCGCCACGCTGCTCTGCGCGCTG 
TTCGTCCGCCATTTCCGCCCGCTGGTGGAAGCCGGCCACGTCTACGTGGCGATGCCGCCGCTGTACCGCATC 
GACCTCGGCAAGGAAATCTACTACGCCCTCGACGAAGCCGAGCGCGACGGCATCCTCGAGCGCCTGGCCGCC 
GAGAAGAAGCGCGGCAAGCCGCAGGTCACCCGCTTCAAGGGCCTTGGCGAAATGAATCCGTTGCAACTGCGC 
GAGACCACCATGGATCCGAATACCCGGCGGCTGGTCCAGCTCACCCTGGAGGACGCCACCGGTACCCTGGAG 
ATCATGGACATGCTGCTGGCCAAGAAGCGCGCCGGTGACCGCAAGTCCTGGCTGGAGAGCAAGGGCAACCTG 
GCCGAGGTGCTGGTCTGA