ViewGene
Locus Name:
GeneID:
Location:

GeneID:422
TrivialName: PA14_65350
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65350
PAO1 Ortholog Locus:PA4946
Sequence Length:1902
Protein Length:633
Start: 5823071
Stop: 5821170
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08331.1|AE004907_9 (AE004907) DNA mismatch repair protein MutL [Pseudomonas aeruginosa PAO1]
Identities = 632/633 (99%), Positives = 632/633 (99%)
Sequence:
ATGAGTGAAACACCGCGTATCCAGCTGCTGAGTCCGCGGCTGGCGAACCAGATCGCCGCCGGTGAGGTGGTC
GAACGTCCCGCCTCGGTGGCCAAGGAACTCCTGGAAAACAGCCTTGACGCCGGTTCCCGGCGCATCGACGTG
GAGGTCGAGCAGGGCGGCATCAAGTTGCTGCGAGTGCGCGACGACGGTCGCGGCATCCCCGCCGACGACCTG
CCGCTGGCCCTGGCTCGCCACGCCACCAGCAAGATCCGCGAGCTGGAAGACCTGGAGCGGGTGATGAGCCTC
GGCTTCCGTGGCGAGGCGCTGGCCTCGATCAGCTCGGTAGCGCGCCTGACCATGACCTCGCGTACCGCCGAC
GCCGGCGAAGCCTGGCAGGTGGAAACCGAGGGCCGCGACATGCAGCCGCGGGTACAGCCGGCGGCGCACCCG
GTGGGGACCAGCGTCGAGGTTCGCGACCTGTTCTTCAACACCCCGGCGCGGCGCAAGTTCCTGCGCGCTGAG
AAGACCGAGTTCGACCATCTGCAGGAAGTCATCAAGCGCCTGGCGCTGGCCCGTTTCGACGTGGCTTTCCAC
CTGCGCCACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGCGCGCGCCGGGTC
GGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAGGTCGAGCGCAACGGCCTGCACCTG
TGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCCAGCCGGACCTGCAGTACTTCTATGTGAACGGGCGC
ATGGTGCGCGACAAGCTGGTCGCCCACGCGGTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCAC
CCGACCTTCGTGCTGTTCTTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAA
GTTCGCTTCCGTGACAGCCGGATGGTCCATGACTTCCTCTATGGCACCTTGCATCGTGCGCTGGGCGAGGTC
CGTCCCGACGACCAGCTCGCGCCGCCCGGGGCGACCAGCCTGACCGAGCCGCGACCGACCGGCGCTGCCGCC
GGCGAGTTCGGCCCGCAGGGCGAGATGCGCCTGGCCGAGAGCGTGCTGGAGAGTCCGGCGGCACGGGTGGGC
TGGAGTGGCGGCTCGTCGGCCTCCGGCGGCTCCTCCGGCTATTCCGCCTACACCCGGCCGGAGGCGCCGCCT
TCTCTGGCCGAGGCCGGTGGCGCCTACAAGGCCTACTTCGCGCCGTTGCCCGCGGGCGAGGCGCCAGCCGCG
TTGCCCGAGAGCGCCCAGGACATCCCGCCGCTGGGTTACGCCCTGGCGCAGCTCAAGGGCATCTACATCCTC
GCCGAGAACGCCCACGGCCTGGTGCTGGTGGACATGCACGCGGCCCACGAACGGATCACCTACGAGCGCCTC
AAGGTGGCGATGGCCAGCGAAGGCTTGCGTGGGCAGCCGTTGCTGGTGCCGGAGTCGATCGCGGTCAGCGAG
CGCGAGGCGGATTGCGCGGAAGAGCATTCCAGCTGGTTCCAGCGCCTGGGCTTCGAGCTGCAGCGCCTGGGG
CCGGAAAGTCTGGCGATCCGGCAGATCCCTGCGCTGCTCAAGCAGGCGGAGGCGACCCAGTTGGTGCGCGAT
GTCATCGCCGACCTGCTGGAGTACGGCACCAGCGACCGTATCCAGGCACACCTGAACGAGTTGCTCGGCACC
ATGGCCTGCCATGGCGCGGTGCGCGCCAACCGGCGCCTGACCCTGCCGGAAATGAACGCCCTGTTGCGCGAC
ATGGAAATCACCGAACGCAGCGGCCAGTGCAACCATGGGCGTCCGACCTGGACCCAGTTGGGCCTGGACGAG
CTGGACAAGCTCTTCCTGCGCGGACGCTGA
Translation:
MSETPRIQLLSPRLANQIAAGEVVERPASVAKELLENSLDAGSRRIDVEVEQGGIKLLRVRDDGRGIPADDL
PLALARHATSKIRELEDLERVMSLGFRGEALASISSVARLTMTSRTADAGEAWQVETEGRDMQPRVQPAAHP
VGTSVEVRDLFFNTPARRKFLRAEKTEFDHLQEVIKRLALARFDVAFHLRHNGKTIFALHEARDELARARRV
GAVCGQAFLEQALPIEVERNGLHLWGWVGLPTFSRSQPDLQYFYVNGRMVRDKLVAHAVRQAYRDVLYNGRH
PTFVLFFEVDPAVVDVNVHPTKHEVRFRDSRMVHDFLYGTLHRALGEVRPDDQLAPPGATSLTEPRPTGAAA
GEFGPQGEMRLAESVLESPAARVGWSGGSSASGGSSGYSAYTRPEAPPSLAEAGGAYKAYFAPLPAGEAPAA
LPESAQDIPPLGYALAQLKGIYILAENAHGLVLVDMHAAHERITYERLKVAMASEGLRGQPLLVPESIAVSE
READCAEEHSSWFQRLGFELQRLGPESLAIRQIPALLKQAEATQLVRDVIADLLEYGTSDRIQAHLNELLGT
MACHGAVRANRRLTLPEMNALLRDMEITERSGQCNHGRPTWTQLGLDELDKLFLRGR*
AnnotationID:431GeneID:422
AnnotatorUID: diggins
Modification Date/Time: 2005-03-13 14:02:53
Gene Name:mutL
Confidence Code:2
GeneProduct:DNA mismatch repair protein MutL
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0323,pfam01119
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58372.1| (AE016873) DNA mismatch repair protein MutL [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 645

 Score = 1022 bits (2642), Expect = 0.0
 Identities = 516/629 (82%), Positives = 559/629 (88%), Gaps = 6/629 (0%)

>gb|AAN70463.1|AE016688_2 (AE016792) DNA mismatch repair protein MutL [Pseudomonas putida
           KT2440]
          Length = 632

 Score = 1020 bits (2638), Expect = 0.0
 Identities = 520/635 (81%), Positives = 562/635 (88%), Gaps = 5/635 (0%)

>gb|AAM37257.1| (AE011877) DNA mismatch repair protein MutL [Xanthomonas axonopodis
           pv. citri str. 306]
          Length = 625

 Score =  636 bits (1640), Expect = 0.0
 Identities = 350/640 (54%), Positives = 433/640 (67%), Gaps = 30/640 (4%)








 
Structural Features:
COG0323, MutL, DNA mismatch repair enzyme (predicted ATPase) 
[DNA replication, recombination, and repair]
CD-Length = 638 residues,  99.8% aligned
Score =  731 bits (1887), Expect = 0.0
Identities = 320/639 (50%), Positives = 405/639 (63%), Gaps = 13/639 (2%)

pfam01119, DNA_mis_repair, DNA mismatch repair protein, C-terminal domain.
CD-Length = 115 residues, 100.0% aligned
Score =  127 bits (321), Expect = 3e-30
Identities = 47/115 (40%), Positives = 66/115 (57%), Gaps = 1/115 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:847




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17989




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35109




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:422_cdd.html


    		




 
URL:

















    ReferenceID:35899




  
Author/Investigator(s):
        Oliver A, Baquero F, Blazquez J.





  
Title:
        The mismatch repair system (mutS, mutL and uvrD genes) in Pseudomonas aeruginosa: molecular characterization of naturally occurring mutants.




  
PubMed:
        11952911


MedLine:
        




    
Source:
Mol Microbiol. 2002 Mar;43(6):1641-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11952911

















    ReferenceID:35900




  
Author/Investigator(s):
        Tsui HC, Zhao G, Feng G, Leung HC, Winkler ME.





  
Title:
        The mutL repair gene of Escherichia coli K-12 forms a superoperon with a gene encoding a new cell-wall amidase.




  
PubMed:
        7511774


MedLine:
        




    
Source:
Mol Microbiol. 1994 Jan;11(1):189-202.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=94195106&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:422
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
712
                           PA4946_tr
                           translation of PA4946
                           633
                           99.84
                           99.84
                           0
                           3237.0
     

     
     
31946
                           gb|AAG08331.1|AE004907_9
                           (AE004907) DNA mismatch repair protein MutL [Pseudomonas aeruginosa PAO1]
                           633
                           99.84
                           99.84
                           0
                           3237.0
     

     
     
31948
                           gb|AAN70463.1|AE016688_2
                           (AE016792) DNA mismatch repair protein MutL [Pseudomonas putida KT2440]
                           638
                           81.66
                           88.24
                           11
                           2648.0
     

     
     
31947
                           gb|AAO58372.1|
                           (AE016873) DNA mismatch repair protein MutL [Pseudomonas syringae pv. tomato str. DC3000]
                           652
                           79.44
                           86.19
                           26
                           2650.0
     

     
     
31949
                           gb|AAM37257.1|
                           (AE011877) DNA mismatch repair protein MutL [Xanthomonas axonopodis pv. citri str. 306]
                           654
                           54.12
                           67.27
                           50
                           1668.0
     

     






      

          
GC ORFID: 43424How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4946Glimmer Score: 

          
Start: 5086752Stop: 5084851

          
Length: 1902


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4946 translation of PA4946Homolog Bit Score 1251.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3428 (ORF: BLASTX 1016.0), t_PA4946 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGTGAAACACCGCGTATCCAGCTGCTGAGTCCGCGGCTGGCGAACCAGATCGCCGCCGGTGAGGTGGTC 
GAACGTCCCGCCTCGGTGGCCAAGGAACTCCTGGAAAACAGCCTTGACGCCGGTTCCCGGCGCATCGACGTG 
GAGGTCGAGCAGGGCGGCATCAAGTTGCTGCGAGTGCGCGACGACGGTCGCGGCATCCCCGCCGACGACCTG 
CCGCTGGCCCTGGCTCGCCACGCCACCAGCAAGATCCGCGAGCTGGAAGACCTGGAGCGGGTGATGAGCCTC 
GGCTTCCGTGGCGAGGCGCTGGCCTCGATCAGCTCGGTAGCGCGCCTGACCATGACCTCGCGTACCGCCGAC 
GCCGGCGAAGCCTGGCAGGTGGAAACCGAGGGCCGCGACATGCAGCCGCGGGTACAGCCGGCGGCGCACCCG 
GTGGGGACCAGCGTCGAGGTTCGCGACCTGTTCTTCAACACCCCGGCGCGGCGCAAGTTCCTGCGCGCTGAG 
AAGACCGAGTTCGACCATCTGCAGGAAGTCATCAAGCGCCTGGCGCTGGCCCGTTTCGACGTGGCTTTCCAC 
CTGCGCCACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGCGCGCGCCGGGTC 
GGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAGGTCGAGCGCAACGGCCTGCACCTG 
TGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCCAGCCGGACCTGCAGTACTTCTATGTGAACGGGCGC 
ATGGTGCGCGACAAGCTGGTCGCCCACGCGGTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCAC 
CCGACCTTCGTGCTGTTCTTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAA 
GTTCGCTTCCGTGACAGCCGGATGGTCCATGACTTCCTCTATGGCACCTTGCATCGTGCGCTGGGCGAGGTC 
CGTCCCGACGACCAGCTCGCGCCGCCCGGGGCGACCAGCCTGACCGAGCCGCGACCGACCGGCGCTGCCGCC 
GGCGAGTTCGGCCCGCAGGGCGAGATGCGCCTGGCCGAGAGCGTGCTGGAGAGTCCGGCGGCACGGGTGGGC 
TGGAGTGGCGGCTCGTCGGCCTCCGGCGGCTCCTCCGGCTATTCCGCCTACACCCGGCCGGAGGCGCCGCCT 
TCTCTGGCCGAGGCCGGTGGCGCCTACAAGGCCTACTTCGCGCCGTTGCCCGCGGGCGAGGCGCCAGCCGCG 
TTGCCCGAGAGCGCCCAGGACATCCCGCCGCTGGGTTACGCCCTGGCGCAGCTCAAGGGCATCTACATCCTC 
GCCGAGAACGCCCACGGCCTGGTGCTGGTGGACATGCACGCGGCCCACGAACGGATCACCTACGAGCGCCTC 
AAGGTGGCGATGGCCAGCGAAGGCTTGCGTGGGCAGCCGTTGCTGGTGCCGGAGTCGATCGCGGTCAGCGAG 
CGCGAGGCGGATTGCGCGGAAGAGCATTCCAGCTGGTTCCAGCGCCTGGGCTTCGAGCTGCAGCGCCTGGGG 
CCGGAAAGTCTGGCGATCCGGCAGATCCCTGCGCTGCTCAAGCAGGCGGAGGCGACCCAGTTGGTGCGCGAT 
GTCATCGCCGACCTGCTGGAGTACGGCACCAGCGACCGTATCCAGGCACACCTGAACGAGTTGCTCGGCACC 
ATGGCCTGCCATGGCGCGGTGCGCGCCAACCGGCGCCTGACCCTGCCGGAAATGAACGCCCTGTTGCGCGAC 
ATGGAAATCACCGAACGCAGCGGCCAGTGCAACCATGGGCGTCCGACCTGGACCCAGTTGGGCCTGGACGAG 
CTGGACAAGCTCTTCCTGCGCGGACGCTGA