ViewGene
Locus Name:
GeneID:
Location:

GeneID:4218
TrivialName: PA14_16440
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_16440
PAO1 Ortholog Locus:PA3707
Sequence Length:516
Protein Length:171
Start: 1407278
Stop: 1407793
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07094.1|AE004789_14 (AE004789) hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 170/171 (99%), Positives = 170/171 (99%)
Sequence:
GTGACCGTGTCCCGCTCCACCGGCGATCATCGACGCACCAGCAAGCTGTTCCTGCTGTTCCGCATGGAAGGC
GACCGCTATGCCCTGGATGCCCGCGAAGTGGTCGAAGTGCTGCCGCTGCTGCGCCTGAAGCGCATTCCGGAG
GCGCCGGAATGGGTCGCCGGGGTGTTCTCCCATCGCGGCGTCCTGGTCCCGGTGCTCGACCTCTGCGCAATG
GCCTTCGGCCGCGCGGCGCTGGCCCGCACCAGCACGCGCATCGTGCTGGTCGAATACCGCGCCCGCCAGGAC
CGAGAGCCGGTCTGGCTCGGACTGATCCTCGAACAGGCCACCGATACCCTGCGCTGCGAACCGTCGGCGTTC
CGCGACTATGGCCTGGACAACGGCGGGGCCCGCTATCTCGGCCCGGTCTATGAAGGACCACGGGGACTGGTG
CAATGGGTGCGGGTCGAGGCATTGCTGCCCGACGAAGTGCGCGCCCTGCTGTTCCCGCCGGAGTGCGGCGAG
GGGGCGGCATGA
Translation:
MTVSRSTGDHRRTSKLFLLFRMEGDRYALDAREVVEVLPLLRLKRIPEAPEWVAGVFSHRGVLVPVLDLCAM
AFGRAALARTSTRIVLVEYRARQDREPVWLGLILEQATDTLRCEPSAFRDYGLDNGGARYLGPVYEGPRGLV
QWVRVEALLPDEVRALLFPPECGEGAA*
AnnotationID:4225GeneID:4218
AnnotatorUID: danlee
Modification Date/Time: 2006-02-02 14:02:25
Gene Name:
Confidence Code:4
GeneProduct:hypothetical protein
Cell Localization:()
Synonyms:wspB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:putative CheW domain protein
Functional Category Confidence Code:5
COGs:COG0835,cd00732
Secondary Functional Category(ies):Chemotaxis
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO92334.1| (AY074937) WspB [Pseudomonas fluorescens]
          Length = 170

 Score =  177 bits (448), Expect = 3e-44
 Identities = 91/150 (60%), Positives = 108/150 (72%)

>gb|AAO55015.1| (AE016861) CheW domain protein [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 178

 Score =  170 bits (430), Expect = 4e-42
 Identities = 87/159 (54%), Positives = 110/159 (69%)

>gb|AAL27610.1|AF430345_2 (AF430345) putative purine-binding chemotaxis protein CheW3
           [Pseudomonas putida]
          Length = 168

 Score =  159 bits (403), Expect = 5e-39
 Identities = 83/156 (53%), Positives = 105/156 (67%), Gaps = 5/156 (3%)






 
Structural Features:
COG0835, CheW, Chemotaxis signal transduction protein 
[Cell motility and secretion / Signal transduction mechanisms]
CD-Length = 165 residues,  98.2% aligned
Score =  109 bits (275), Expect = 3e-25
Identities = 46/168 (27%), Positives = 74/168 (44%), Gaps = 8/168 (4%)

cd00732, CheW, CheW, a small regulator protein, unique 
to the chemotaxis signalling in prokaryotes and archea.
CD-Length = 140 residues,  97.1% aligned
Score = 72.9 bits (179), Expect = 4e-14
Identities = 36/143 (25%), Positives = 67/143 (46%), Gaps = 10/143 (6%)







   
Genomic Context:






   
Comment:


















    ReferenceID:15040




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25579




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31844




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4218_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:4218
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6504
                           PA3707_tr
                           translation of PA3707
                           171
                           99.41
                           99.41
                           0
                           881.0
     

     
     
176712
                           gb|AAG07094.1|AE004789_14
                           (AE004789) hypothetical protein PA3707 [Pseudomonas aeruginosa PAO1]
                           171
                           98.83
                           99.41
                           0
                           878.0
     

     
     
176713
                           gb|AAO92334.1|
                           (AY074937) WspB [Pseudomonas fluorescens]
                           181
                           50.82
                           61.32
                           21
                           437.0
     

     
     
176714
                           gb|AAO55015.1|
                           (AE016861) CheW domain protein [Pseudomonas syringae pv. tomato str. DC3000]
                           178
                           50.00
                           64.04
                           7
                           416.0
     

     
     
176716
                           gb|AAL27610.1|AF430345_2
                           (AF430345) putative purine-binding chemotaxis protein CheW3 [Pseudomonas putida]
                           174
                           48.27
                           61.49
                           9
                           390.0
     

     






      

          
GC ORFID: 47220How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3707Glimmer Score: 

          
Start: 3546659Stop: 3546144

          
Length: 516


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3707 translation of PA3707Homolog Bit Score 343.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4582 (ORF: BLASTX 171.0), t_PA3707 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGACCGTGTCCCGCTCCACCGGCGATCATCGACGCACCAGCAAGCTGTTCCTGCTGTTCCGCATGGAAGGC 
GACCGCTATGCCCTGGATGCCCGCGAAGTGGTCGAAGTGCTGCCGCTGCTGCGCCTGAAGCGCATTCCGGAG 
GCGCCGGAATGGGTCGCCGGGGTGTTCTCCCATCGCGGCGTCCTGGTCCCGGTGCTCGACCTCTGCGCAATG 
GCCTTCGGCCGCGCGGCGCTGGCCCGCACCAGCACGCGCATCGTGCTGGTCGAATACCGCGCCCGCCAGGAC 
CGAGAGCCGGTCTGGCTCGGACTGATCCTCGAACAGGCCACCGATACCCTGCGCTGCGAACCGTCGGCGTTC 
CGCGACTATGGCCTGGACAACGGCGGGGCCCGCTATCTCGGCCCGGTCTATGAAGGACCACGGGGACTGGTG 
CAATGGGTGCGGGTCGAGGCATTGCTGCCCGACGAAGTGCGCGCCCTGCTGTTCCCGCCGGAGTGCGGCGAG 
GGGGCGGCATGA