ViewGene
Locus Name:
GeneID:
Location:

GeneID:4208
TrivialName: PA14_64650
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_64650
PAO1 Ortholog Locus:PA4891
Sequence Length:504
Protein Length:167
Start: 5758621
Stop: 5759124
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08276.1|AE004902_4 (AE004902) urease accessory protein UreE [Pseudomonas aeruginosa PAO1]
Identities = 166/167 (99%), Positives = 166/167 (99%)
Sequence:
ATGCTGGTCATTCACCAACGCCTGCCCGCCCGCTCGCCCCGCTGGGACGAAGAACTCCACCTCACCTACGAG
GCGCGCAGCAAAAGCCGCCTGCGCTGCTTCGCGGCGAGCGGCGAGGAGGTCGGCCTGTTCCTCGAGCGCGGC
CAGCCGCCGCTGGCCGACGGCGACTGCCTGGAGGCCCGCGACGGTCGCCTGGTGCGGGTCGTCGCCCGCCCC
GAGCGTCTGCTCCACGTCACCTGCGCCAGCCCGCTGGAACTGACCCGCGCCGCCTACCACCTGGGCAACCGT
CACGTCGCCCTGCAGGTCGGCGACGGCTGGCTGCGCCTGCTCGACGACTACGTGCTGAAAGCCATGCTCGAA
CAGCTCGGCGCCACGGTGGAGGCCATCGAGGCGCCGTTCCAGCCGGAGCACGGCGCCTACGGCGGAGGCCAC
CACCACTCGCACCATGGCGAGGCGGAGTTCAACTACGCGCCGCGCCTGCACCAGTTCGGCGTGCGCCGGTGA
Translation:
MLVIHQRLPARSPRWDEELHLTYEARSKSRLRCFAASGEEVGLFLERGQPPLADGDCLEARDGRLVRVVARP
ERLLHVTCASPLELTRAAYHLGNRHVALQVGDGWLRLLDDYVLKAMLEQLGATVEAIEAPFQPEHGAYGGGH
HHSHHGEAEFNYAPRLHQFGVRR*
AnnotationID:4215GeneID:4208
AnnotatorUID: diggins
Modification Date/Time: 2005-03-11 12:12:32
Gene Name:ureE
Confidence Code:2
GeneProduct:urease accessory protein UreE
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2371,cd00571
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58337.1| (AE016873) urease accessory protein UreE [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 166

 Score =  271 bits (693), Expect = 1e-72
 Identities = 128/167 (76%), Positives = 145/167 (86%), Gaps = 1/167 (0%)

>gb|AAL86692.1|AF417006_4 (AF417006) urease accessory protein [Nitrosococcus oceani]
          Length = 168

 Score =  153 bits (387), Expect = 3e-37
 Identities = 81/166 (48%), Positives = 102/166 (61%), Gaps = 3/166 (1%)

>gb|AAN76658.1|AF411008_4 (AF411008) UreE [Nitrosospira sp. NpAV]
          Length = 181

 Score =  134 bits (336), Expect = 3e-31
 Identities = 73/139 (52%), Positives = 90/139 (64%), Gaps = 5/139 (3%)






 
Structural Features:
COG2371, UreE, Urease accessory protein UreE 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 155 residues,  98.7% aligned
Score =  156 bits (396), Expect = 1e-39
Identities = 76/154 (49%), Positives = 96/154 (62%), Gaps = 6/154 (3%)

cd00571, UreE, UreE urease accessory protein.
CD-Length = 136 residues,  85.3% aligned
Score =  119 bits (300), Expect = 2e-28








   
Genomic Context:






   
Comment:


















    ReferenceID:8418




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25559




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35939




  
Author/Investigator(s):
        de Koning-Ward TF, Ward AC, Robins-Browne RM.





  
Title:
        Characterisation of the urease-encoding gene complex of Yersinia enterocolitica.




  
PubMed:
        8045421


MedLine:
        




    
Source:
Gene. 1994 Jul 22;145(1):25-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8045421

















    ReferenceID:35943




  
Author/Investigator(s):
        Mulrooney SB, Hausinger RP.





  
Title:
        Sequence of the Klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation.




  
PubMed:
        2211515


MedLine:
        




    
Source:
J Bacteriol. 1990 Oct;172(10):5837-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2211515

















    ReferenceID:35945




  
Author/Investigator(s):
        Jones BD, Mobley HL.





  
Title:
        Proteus mirabilis urease: nucleotide sequence determination and comparison with jack bean urease.




  
PubMed:
        2687233


MedLine:
        




    
Source:
J Bacteriol. 1989 Dec;171(12):6414-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2687233

















    ReferenceID:35953




  
Author/Investigator(s):
        Maeda M, Hidaka M, Nakamura A, Masaki H, Uozumi T.





  
Title:
        Cloning, sequencing, and expression of thermophilic Bacillus sp. strain TB-90 urease gene complex in Escherichia coli.




  
PubMed:
        8288539


MedLine:
        




    
Source:
J Bacteriol. 1994 Jan;176(2):432-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8288539

















    ReferenceID:35967




  
Author/Investigator(s):
        Zhu J, Teng CH, Chang CF, Chang CD, Simpson KW, Wei C, McDonough P, McDonough S, Chang YF.





  
Title:
        Cloning and characterization of a Helicobacter bizzozeronii urease gene cluster.




  
PubMed:
        12652903


MedLine:
        




    
Source:
DNA Seq. 2002 Dec;13(6):321-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12652903

















    ReferenceID:38065




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4208_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:4208
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6494
                           PA4891_tr
                           translation of PA4891
                           167
                           99.40
                           99.40
                           0
                           884.0
     

     
     
176639
                           gb|AAG08276.1|AE004902_4
                           (AE004902) urease accessory protein UreE [Pseudomonas aeruginosa PAO1]
                           167
                           99.40
                           99.40
                           0
                           884.0
     

     
     
176640
                           gb|AAO58337.1|
                           (AE016873) urease accessory protein UreE [Pseudomonas syringae pv. tomato str. DC3000]
                           167
                           76.64
                           86.82
                           1
                           695.0
     

     
     
176641
                           gb|AAL86692.1|AF417006_4
                           (AF417006) urease accessory protein [Nitrosococcus oceani]
                           170
                           49.41
                           62.35
                           5
                           389.0
     

     
     
176645
                           gb|AAN68454.1|AE016478_6
                           (AE016784) urease accessory protein UreE [Pseudomonas putida KT2440]
                           177
                           45.19
                           54.80
                           20
                           302.0
     

     






      

          
GC ORFID: 47210How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4891Glimmer Score: 

          
Start: 5022302Stop: 5022805

          
Length: 504


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4891 translation of PA4891Homolog Bit Score 345.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5822 (ORF: BLASTX 271.0), t_PA4891 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTGGTCATTCACCAACGCCTGCCCGCCCGCTCGCCCCGCTGGGACGAAGAACTCCACCTCACCTACGAG 
GCGCGCAGCAAAAGCCGCCTGCGCTGCTTCGCGGCGAGCGGCGAGGAGGTCGGCCTGTTCCTCGAGCGCGGC 
CAGCCGCCGCTGGCCGACGGCGACTGCCTGGAGGCCCGCGACGGTCGCCTGGTGCGGGTCGTCGCCCGCCCC 
GAGCGTCTGCTCCACGTCACCTGCGCCAGCCCGCTGGAACTGACCCGCGCCGCCTACCACCTGGGCAACCGT 
CACGTCGCCCTGCAGGTCGGCGACGGCTGGCTGCGCCTGCTCGACGACTACGTGCTGAAAGCCATGCTCGAA 
CAGCTCGGCGCCACGGTGGAGGCCATCGAGGCGCCGTTCCAGCCGGAGCACGGCGCCTACGGCGGAGGCCAC 
CACCACTCGCACCATGGCGAGGCGGAGTTCAACTACGCGCCGCGCCTGCACCAGTTCGGCGTGCGCCGGTGA