ViewGene
Locus Name:
GeneID:
Location:

GeneID:4200
TrivialName: PA14_47680
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_47680
PAO1 Ortholog Locus:PA1278
Sequence Length:522
Protein Length:173
Start: 4244889
Stop: 4244368
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04667.1|AE004557_8 (AE004557) cobinamide kinase [Pseudomonas aeruginosa PAO1]
Identities = 172/173 (99%), Positives = 173/173 (100%)
Sequence:
ATGCGTGACCTGATCCTTGGCGGCGCCCGTTCCGGCAAGAGCCGCCTGGCCGAGCGCCTGGCCGCCGAGAGC
GGGCTGGCGGTGAGCTACATCGCCACCGCCCAGGCCGGCGACGGCGAAATGGGCCGGCGCATCGCCGAGCAC
CGCGCGCGCCGCCCGGCCCACTGGCGAACCCTGGAAGAGCCCCTGGCGCTGGCGGCGACGCTGCGCAGCGAG
GCCGAGGCCGGACGCTGCCTGCTGGTCGACTGCCTGACCCTGTGGCTGACCAACCTGCTGCTCTGCGACGAT
CCCCAGCGTCTCGACGGCGAGCGCGAGGCGCTGCTGGAATGTCTCGGCGAGCTGCCGGGGCGGATCATCCTG
GTCAGCAACGAAACCGGGCTCGGCGTGGTGCCGCTGGGCGAACTCAGCCGGCGCTACGTCGACGAGGCCGGC
TGGCTGCACCAGGCCCTCGCCGAACGCTGCGAACGGGTGACCTTCACCGTCGCCGGCCTGCCCATGCCGTTG
AAAGGAGAACCGCTATGA
Translation:
MRDLILGGARSGKSRLAERLAAESGLAVSYIATAQAGDGEMGRRIAEHRARRPAHWRTLEEPLALAATLRSE
AEAGRCLLVDCLTLWLTNLLLCDDPQRLDGEREALLECLGELPGRIILVSNETGLGVVPLGELSRRYVDEAG
WLHQALAERCERVTFTVAGLPMPLKGEPL*
AnnotationID:4207GeneID:4200
AnnotatorUID: diggins
Modification Date/Time: 2005-02-22 18:06:05
Gene Name:cobP
Confidence Code:2
GeneProduct:cobinamide kinase/cobinamide phosphate guanylyltransferase
Cell Localization:()
Synonyms:cobU
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:adenosyl cobinamide kinase
Functional Category Confidence Code:5
COGs:COG2087,cd00544,pfam02283
Secondary Functional Category(ies):coenzyme metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67299.1|AE016356_7 (AE016780) cobinamide kinase [Pseudomonas putida KT2440]
          Length = 173

 Score =  250 bits (639), Expect = 2e-66
 Identities = 126/173 (72%), Positives = 144/173 (83%)

>gb|AAO55234.1| (AE016861) cobinamide kinase/cobinamide phosphate
           guanylyltransferase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 173

 Score =  250 bits (638), Expect = 3e-66
 Identities = 127/173 (73%), Positives = 142/173 (82%)

>gb|AAM42331.1| (AE012420) adenosyl cobinamide kinase/adenosyl cobinamide phosphate
           guanylyltransferase [Xanthomonas campestris pv.
           campestris str. ATCC 33913]
          Length = 173

 Score =  233 bits (593), Expect = 5e-61
 Identities = 119/171 (69%), Positives = 137/171 (80%)






 
Structural Features:
COG2087, CobU, Adenosyl cobinamide kinase/adenosyl cobinamide 
phosphate guanylyltransferase 
[Coenzyme metabolism]
CD-Length = 175 residues, 100.0% aligned
Score =  221 bits (565), Expect = 3e-59
Identities = 94/175 (53%), Positives = 111/175 (63%), Gaps = 5/175 (2%)

cd00544, CobU, Adenosylcobinamide kinase / 
adenosylcobinamide phosphate guanyltransferase (CobU).
CD-Length = 169 residues,  98.8% aligned
Score =  189 bits (482), Expect = 1e-49

pfam02283, CobU, Cobinamide kinase / 
cobinamide phosphate guanyltransferase. 
CD-Length = 166 residues,  99.4% aligned
Score =  163 bits (413), Expect = 1e-41
Identities = 86/166 (51%), Positives = 107/166 (64%), Gaps = 2/166 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8402




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25543




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37513




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4200_cdd.html


    		




 
URL:

















    ReferenceID:38032




  
Author/Investigator(s):
        Crouzet J, Levy-Schil S, Cameron B, Cauchois L, Rigault S, Rouyez MC, Blanche F, Debussche L, Thibaut D.





  
Title:
        Nucleotide sequence and genetic analysis of a 13.1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and bifunctional cob




  
PubMed:
        1655697


MedLine:
        




    
Source:
J Bacteriol. 1991 Oct;173(19):6074-87.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1655697









           
Homologs By Global Alignment 

           

       



     
Gene ID:4200
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6483
                           PA1278_tr
                           translation of PA1278
                           173
                           99.42
                           99.99
                           0
                           885.0
     

     
     
176548
                           gb|AAG04667.1|AE004557_8
                           (AE004557) cobinamide kinase [Pseudomonas aeruginosa PAO1]
                           173
                           99.42
                           99.99
                           0
                           885.0
     

     
     
176550
                           gb|AAO55234.1|
                           (AE016861) cobinamide kinase/cobinamide phosphate guanylyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           173
                           73.41
                           82.08
                           0
                           638.0
     

     
     
176549
                           gb|AAN67299.1|AE016356_7
                           (AE016780) cobinamide kinase [Pseudomonas putida KT2440]
                           173
                           72.83
                           83.23
                           0
                           639.0
     

     
     
176551
                           gb|AAM42331.1|
                           (AE012420) adenosyl cobinamide kinase/adenosyl cobinamide phosphate guanylyltransferase [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           173
                           68.78
                           79.76
                           0
                           593.0
     

     






      

          
GC ORFID: 47202How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1278Glimmer Score: 

          
Start: 709020Stop: 709541

          
Length: 522


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1278 translation of PA1278Homolog Bit Score 345.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6474 (ORF: BLASTX 242.0), t_PA1278 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGTGACCTGATCCTTGGCGGCGCCCGTTCCGGCAAGAGCCGCCTGGCCGAGCGCCTGGCCGCCGAGAGC 
GGGCTGGCGGTGAGCTACATCGCCACCGCCCAGGCCGGCGACGGCGAAATGGGCCGGCGCATCGCCGAGCAC 
CGCGCGCGCCGCCCGGCCCACTGGCGAACCCTGGAAGAGCCCCTGGCGCTGGCGGCGACGCTGCGCAGCGAG 
GCCGAGGCCGGACGCTGCCTGCTGGTCGACTGCCTGACCCTGTGGCTGACCAACCTGCTGCTCTGCGACGAT 
CCCCAGCGTCTCGACGGCGAGCGCGAGGCGCTGCTGGAATGTCTCGGCGAGCTGCCGGGGCGGATCATCCTG 
GTCAGCAACGAAACCGGGCTCGGCGTGGTGCCGCTGGGCGAACTCAGCCGGCGCTACGTCGACGAGGCCGGC 
TGGCTGCACCAGGCCCTCGCCGAACGCTGCGAACGGGTGACCTTCACCGTCGCCGGCCTGCCCATGCCGTTG 
AAAGGAGAACCGCTATGA