ViewGene
Locus Name:
GeneID:
Location:

GeneID:4153
TrivialName: PA14_17100
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17100
PAO1 Ortholog Locus:PA3653
Sequence Length:558
Protein Length:185
Start: 1465791
Stop: 1466348
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07041.1|AE004785_5 (AE004785) ribosome recycling factor [Pseudomonas aeruginosa PAO1]
Identities = 185/185 (100%), Positives = 185/185 (100%)
Sequence:
ATGATCAACGAGATCAAGAAGGAAGCGCAGGAGCGCATGGGCAAGACCCTGGAAGCGCTGGGCCATGCCTTC
GCGAAGATTCGCACCGGTCGCGCGCATCCGAGCATCCTGGATAGCGTGATGGTTTCCTATTACGGTGCCGAT
ACGCCGCTGCGCCAGGTCGCCAACGTCACCGTGGAAGACTCCCGCACCCTGGCCCTGGCCGTGTTCGACAAG
AGCATGATCcAGGCGGTCGAGAAGGCcATCATGACCTCCGACCTGGGGCTCAACCCGGCCACCGCCGGCACC
ACCATCCGCGTACCGATGCCGGCCCTGACCGAGGAGACCCGCAAGGGCTACACCAAGCAGGCGCGTGCCGAG
GCCGAGCAGGCGCGGGTTTCCGTGCGCAACATCCGTCGCGATGCGCTGGCCCAGTTGAAGGACCTGCAGAAG
GAAAAGGAAATCAGCGAGGACGaaGAGCGCCGCGCCGGCGACGACGTGCAGAAGCTGACCGACAAGTTCATC
GGTGAGATCGAGAAGGCACTGGAAGCCAAAGAAGCGGACCTCATGGCTGTCTGA
Translation:
MINEIKKEAQERMGKTLEALGHAFAKIRTGRAHPSILDSVMVSYYGADTPLRQVANVTVEDSRTLALAVFDK
SMIQAVEKAIMTSDLGLNPATAGTTIRVPMPALTEETRKGYTKQARAEAEQARVSVRNIRRDALAQLKDLQK
EKEISEDEERRAGDDVQKLTDKFIGEIEKALEAKEADLMAV*
AnnotationID:4160GeneID:4153
AnnotatorUID: diggins
Modification Date/Time: 2005-04-21 05:05:00
Gene Name:frr
Confidence Code:1
GeneProduct:ribosome recycling factor
Cell Localization:()
Synonyms:rrf
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:ribosome releasing factor
Functional Category Confidence Code:5
COGs:COG0233,cd00520,pfam01765
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67215.1|AE016348_7 (AE016779) ribosome recycling factor [Pseudomonas putida KT2440]
          Length = 185

 Score =  260 bits (665), Expect = 3e-69
 Identities = 130/185 (70%), Positives = 157/185 (84%)

>gb|AAN78702.1|AE016755_202 (AE016755) Ribosome recycling factor [Escherichia coli CFT073]
          Length = 185

 Score =  224 bits (572), Expect = 2e-58
 Identities = 118/183 (64%), Positives = 145/183 (79%)






 
Structural Features:
COG0233, Frr, Ribosome recycling factor 
[Translation, ribosomal structure and biogenesis]
CD-Length = 187 residues,  98.9% aligned
Score =  235 bits (600), Expect = 7e-63
Identities = 103/185 (55%), Positives = 146/185 (78%)

cd00520, RRF, Ribosome recycling factor (RRF).
CD-Length = 179 residues, 100.0% aligned
Score =  209 bits (534), Expect = 3e-55
Identities = 100/179 (55%), Positives = 139/179 (77%)

pfam01765, RRF, Ribosome recycling factor.
CD-Length = 165 residues, 100.0% aligned
Score =  194 bits (495), Expect = 9e-51
Identities = 92/165 (55%), Positives = 128/165 (77%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8308




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25451




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31890




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4153_cdd.html


    		




 
URL:

















    ReferenceID:34220




  
Author/Investigator(s):
        Ohnishi M, Janosi L, Shuda M, Matsumoto H, Hayashi T, Terawaki Y, Kaji A.





  
Title:
        Molecular cloning, sequencing, purification, and characterization of Pseudomonas aeruginosa ribosome recycling factor.




  
PubMed:
        9973356


MedLine:
        




    
Source:
J Bacteriol. 1999 Feb;181(4):1281-91.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9973356









           
Homologs By Global Alignment 

           

       



     
Gene ID:4153
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6418
                           PA3653_tr
                           translation of PA3653
                           185
                           99.99
                           99.99
                           0
                           904.0
     

     
     
175759
                           gb|AAG07041.1|AE004785_5
                           (AE004785) ribosome recycling factor [Pseudomonas aeruginosa PAO1]
                           185
                           99.99
                           99.99
                           0
                           904.0
     

     
     
175760
                           dbj|BAA32345.1|
                           (AB010087) ribosome recycling factor [Pseudomonas aeruginosa]
                           185
                           99.99
                           99.99
                           0
                           904.0
     

     
     
175762
                           gb|AAO55057.1|
                           (AE016861) ribosome recycling factor [Pseudomonas syringae pv. tomato str. DC3000]
                           185
                           71.35
                           83.78
                           0
                           663.0
     

     
     
175761
                           gb|AAN67215.1|AE016348_7
                           (AE016779) ribosome recycling factor [Pseudomonas putida KT2440]
                           185
                           70.27
                           84.86
                           0
                           665.0
     

     






      

          
GC ORFID: 47155How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3653Glimmer Score: 

          
Start: 3488146Stop: 3487589

          
Length: 558


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3653 translation of PA3653Homolog Bit Score 352.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4622 (ORF: BLASTX 262.0), t_PA3653 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCAACGAGATCAAGAAGGAAGCGCAGGAGCGCATGGGCAAGACCCTGGAAGCGCTGGGCCATGCCTTC 
GCGAAGATTCGCACCGGTCGCGCGCATCCGAGCATCCTGGATAGCGTGATGGTTTCCTATTACGGTGCCGAT 
ACGCCGCTGCGCCAGGTCGCCAACGTCACCGTGGAAGACTCCCGCACCCTGGCCCTGGCCGTGTTCGACAAG 
AGCATGATCcAGGCGGTCGAGAAGGCcATCATGACCTCCGACCTGGGGCTCAACCCGGCCACCGCCGGCACC 
ACCATCCGCGTACCGATGCCGGCCCTGACCGAGGAGACCCGCAAGGGCTACACCAAGCAGGCGCGTGCCGAG 
GCCGAGCAGGCGCGGGTTTCCGTGCGCAACATCCGTCGCGATGCGCTGGCCCAGTTGAAGGACCTGCAGAAG 
GAAAAGGAAATCAGCGAGGACGaaGAGCGCCGCGCCGGCGACGACGTGCAGAAGCTGACCGACAAGTTCATC 
GGTGAGATCGAGAAGGCACTGGAAGCCAAAGAAGCGGACCTCATGGCTGTCTGA