ViewGene
Locus Name:
GeneID:
Location:

GeneID:4150
TrivialName: PA14_04650
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_04650
PAO1 Ortholog Locus:PA0355
Sequence Length:540
Protein Length:179
Start: 414192
Stop: 413653
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03744.1|AE004473_6 (AE004473) protease PfpI [Pseudomonas aeruginosa PAO1]
Identities = 177/179 (98%), Positives = 178/179 (99%)
Sequence:
ATGACCCAATCCCTGCACGGCAAGGTCGTCGCCGCCCTGGTGACCGACGGTTTCGAACAGGTCGAACTGACC
GGTCCGAAAAAAGCCCTGGAAGACGCCGGCGCCACGGTAAGGATTCTTTCCGCCAAGGCCGGCGAGGTGCGC
GGCTGGAACCATCACCAGCCAGCCGACGCCTTCCGCGTCGACGGCACCTTCGAAGACGCCAGCCTGGACGAC
TACGACGCCCTGCTGCTGCCCGGCGGGGTGATCAATTCCGACCAGATCCGCAGCCTGGCCAAGGCCCAGGAA
CTGGCGATCCGCGCCGAACAGGCGAGCAAGCCGGTGGCGGTGATCTGCCACGGCGCCTGGCTGCTGATCTCC
GCCGGCCTGGTGCAGGGTCGTACCCTGACCAGTTGGCCGTCGCTCAAGGACGACATCAACAACGCCGGCGGC
CACTGGGTGGACCAGGAGGTAGCGGTGGACGGCAAGCTGGTGAGCAGCCGCAAACCCGAAGACATCCCGGCC
TTCAACCGTCGATTCATCGAAATCCTCGCCGGCTGA
Translation:
MTQSLHGKVVAALVTDGFEQVELTGPKKALEDAGATVRILSAKAGEVRGWNHHQPADAFRVDGTFEDASLDD
YDALLLPGGVINSDQIRSLAKAQELAIRAEQASKPVAVICHGAWLLISAGLVQGRTLTSWPSLKDDINNAGG
HWVDQEVAVDGKLVSSRKPEDIPAFNRRFIEILAG*
AnnotationID:4157GeneID:4150
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 18:06:50
Gene Name:pfpI
Confidence Code:2
GeneProduct:protease PfpI
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0693
Secondary Functional Category(ies):
EC Number:3.2.-.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68333.1|AE016467_3 (AE016784) protease PfpI [Pseudomonas putida KT2440]
          Length = 180

 Score =  232 bits (592), Expect = 7e-61
 Identities = 113/178 (63%), Positives = 142/178 (79%)

>gb|AAM42035.1| (AE012389) protease [Xanthomonas campestris pv. campestris str.
           ATCC 33913]
          Length = 180

 Score =  188 bits (477), Expect = 2e-47
 Identities = 94/179 (52%), Positives = 119/179 (66%), Gaps = 1/179 (0%)








 
Structural Features:
COG0693, ThiJ, Putative intracellular protease/amidase
CD-Length = 188 residues,  97.9% aligned
Score =  116 bits (292), Expect = 1e-27
Identities = 64/185 (34%), Positives = 90/185 (48%), Gaps = 12/185 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8302




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25445




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33695




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4150_cdd.html


    		




 
URL:

















    ReferenceID:35407




  
Author/Investigator(s):
        Halio SB, Blumentals II, Short SA, Merrill BM, Kelly RM.





  
Title:
        Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus.




  
PubMed:
        8626329


MedLine:
        




    
Source:
J Bacteriol. 1996 May;178(9):2605-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8626329









           
Homologs By Global Alignment 

           

       



     
Gene ID:4150
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6413
                           PA0355_tr
                           translation of PA0355
                           179
                           98.88
                           99.44
                           0
                           907.0
     

     
     
175634
                           gb|AAG03744.1|AE004473_6
                           (AE004473) protease PfpI [Pseudomonas aeruginosa PAO1]
                           179
                           98.88
                           99.44
                           0
                           907.0
     

     
     
175635
                           gb|AAN68333.1|AE016467_3
                           (AE016784) protease PfpI [Pseudomonas putida KT2440]
                           180
                           62.77
                           78.88
                           1
                           591.0
     

     
     
175636
                           gb|AAM42035.1|
                           (AE012389) protease [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           181
                           54.14
                           67.95
                           3
                           480.0
     

     
     
175638
                           gb|AAM37777.1|
                           (AE011935) protease [Xanthomonas axonopodis pv. citri str. 306]
                           182
                           51.64
                           66.48
                           5
                           456.0
     

     






      

          
GC ORFID: 47152How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0355Glimmer Score: 

          
Start: 6215534Stop: 6214995

          
Length: 540


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0355 translation of PA0355Homolog Bit Score 353.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6827 (ORF: BLASTX 239.0), t_PA4171 (ORF: BLASTX 132.0), t_PA0355 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCCAATCCCTGCACGGCAAGGTCGTCGCCGCCCTGGTGACCGACGGTTTCGAACAGGTCGAACTGACC 
GGTCCGAAAAAAGCCCTGGAAGACGCCGGCGCCACGGTAAGGATTCTTTCCGCCAAGGCCGGCGAGGTGCGC 
GGCTGGAACCATCACCAGCCAGCCGACGCCTTCCGCGTCGACGGCACCTTCGAAGACGCCAGCCTGGACGAC 
TACGACGCCCTGCTGCTGCCCGGCGGGGTGATCAATTCCGACCAGATCCGCAGCCTGGCCAAGGCCCAGGAA 
CTGGCGATCCGCGCCGAACAGGCGAGCAAGCCGGTGGCGGTGATCTGCCACGGCGCCTGGCTGCTGATCTCC 
GCCGGCCTGGTGCAGGGTCGTACCCTGACCAGTTGGCCGTCGCTCAAGGACGACATCAACAACGCCGGCGGC 
CACTGGGTGGACCAGGAGGTAGCGGTGGACGGCAAGCTGGTGAGCAGCCGCAAACCCGAAGACATCCCGGCC 
TTCAACCGTCGATTCATCGAAATCCTCGCCGGCTGA