ViewGene
Locus Name:
GeneID:
Location:

GeneID:414
TrivialName: PA14_63530
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_63530
PAO1 Ortholog Locus:PA4807
Sequence Length:1926
Protein Length:641
Start: 5665088
Stop: 5663163
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08193.1|AE004893_11 (AE004893) selenocysteine-specific elongation factor [Pseudomonas aeruginosa PAO1]
Identities = 638/641 (99%), Positives = 639/641 (99%)
Sequence:
GTGATCGTCGGCACCGCCGGGCACATCGACCACGGCAAGACCTCGCTGCTGCGCGCCCTCACCGGCATCGAG
GGCGACCGCCGCCCGGCCGAGCGCCAACGCGGGATCACCATCGACCTGGGCTATCTCTACGCCGACCTTGGC
GACGGCAGCCCCACCGGCTTCATCGACGTGCCCGGTCACGAGCGCTTCGTCCACAACATGCTGGCCGGCGCC
AGCGGCATCGATTGCGTGCTGCTGGTGGTGGCCGCCGACGACGGCCTGATGCCGCAGACCCGCGAGCACCTG
GCGATCGTCGAACTGCTCGGTATCCGCCGGGCGCTGGTGGCGCTGACCAAGATCGACCGGGTCGAGCCGCAG
CGGGTGCAGCAGGTGCGGACCCAGGTCGAACACCTGCTGGCCTCCGGCCCGCTGGCGCGCTCGCCGATCTTC
CCGCTCTCCAGCAGCACCGGGGAGGGCGTCGCGGCGTTGCGCGAAGCACTCGGCGGGCTCGCCGGCGAAGTC
CGCGAGCGTAGCCGCGAGGGATACTTCCGCCTGGCGGTGGACCGGGCCTTCAGCGTCGCCGGCAGCGGCATC
GTGGTCACCGGCACGGCCTTCTCCGGACAGGTCGCGGTGGGCGACGAACTGCTCCTCGGCAACGCCGGACGG
CCGGTGCGGGTGCGCGGCCTGCACGCGCAGAACCGGCCCGCGCAACAGGCCTGGGCCGGCCAGCGCGTGGCC
CTGAACATCGCCGGCGAGCGCCTCGCGCTGGAACAGATCCAGCGCGGCGACTGGCTGCTGCAGGCAGCGCTG
CACGCGCCGACCACGCGGGTCGACATCGACTTCCGCCTGTTGCCCGACGAACTTCGCGACTTCACCCACTGG
ACCCCGGTGCACCTGCACCTGGGCACCCAGGACGTCACCGGGCGCCTGGCCCTGCTCGAAGGCGAACAACTG
GAGCCTGGCCACCGCGCCTTCGCCCAACTGGTGCTGAACGCGCCGATCCAGGCCCTGCACGGCGACCGCCTG
GTGCTGCGCGACCAGTCGGCGCAGCGCACCCTTGGCGGCGGGCGGGTGCTCGACGGCGCCGCGCCGGCGCGC
AACCGACGAACGCCGGCACGTCTGGCACAGCTCCAGGCGCTGCGCCAGGAAAGCCTGGAGGAGGCGCTGCCG
ACCTTGCTCGGCGCGGCCGAGAACGGCCTCGACCCGCAACGCCTGGTGCAATCCTTCAACCGCCCGCGCGAG
CACTGGCGGCTGCCGGAAGAGGTGGTCGAGGTGCAGACCCGCCTCGGCCCCCGGTTGTTCGCCGGGGAGCGC
TGGTTCGCCCTGGTCGAGCAACTGCTCGCCGGCCTGCGGCGCTTCCACGAAGAGCTACCGGACGAACTCGGT
CCCGACCGCGACCGCCTGCGTCGCTACGCCCTGCCGCAGCTGGAGCGGCCGGTGTTCATCGCCTTGCTCGAG
GCCGCCCTGGCCGGCGGCGACGTGCACAGCAGCGGGCCCTGGCTGCACCTGCCCGGCCATGAAGTGCGCCTG
AGCGATAGCGAGGAGCAACTGCGCGCGCGCCTCTGGCCGCTGCTCGAAGCCGGCCGCTACGACCCGCCCTGG
GTTCGCGACCTGGCCCGCGAGCTGCGCCAGGACGAAGCCGCCATGCGCAACCTGCTGCGCAAGCTGGCGCGC
CTGGGCCAGTTGCAGCAGGTGGTGCGCGACCTGTTCTACCCGGAAGCCACCCTGCGTCAATTGGCCGAGATC
GCCGTGCAGGTCCGCGACCCCGCCGGGCTGATCCGCCCGGCGGAATTCCGCGACCGCCTGGGCATCGGCCGC
AAGCGCAGCATCCAGATCCTCGAGCACTTCGACCGCATCGGCCTGACCCGCCGCATCGGCAACGACCGTCGC
CTGCGCGAGGGCTCGGCGCTGGCCGAACAGTTGCTCGGCGGCGTCTCGCCCTGA
Translation:
MIVGTAGHIDHGKTSLLRALTGIEGDRRPAERQRGITIDLGYLYADLGDGSPTGFIDVPGHERFVHNMLAGA
SGIDCVLLVVAADDGLMPQTREHLAIVELLGIRRALVALTKIDRVEPQRVQQVRTQVEHLLASGPLARSPIF
PLSSSTGEGVAALREALGGLAGEVRERSREGYFRLAVDRAFSVAGSGIVVTGTAFSGQVAVGDELLLGNAGR
PVRVRGLHAQNRPAQQAWAGQRVALNIAGERLALEQIQRGDWLLQAALHAPTTRVDIDFRLLPDELRDFTHW
TPVHLHLGTQDVTGRLALLEGEQLEPGHRAFAQLVLNAPIQALHGDRLVLRDQSAQRTLGGGRVLDGAAPAR
NRRTPARLAQLQALRQESLEEALPTLLGAAENGLDPQRLVQSFNRPREHWRLPEEVVEVQTRLGPRLFAGER
WFALVEQLLAGLRRFHEELPDELGPDRDRLRRYALPQLERPVFIALLEAALAGGDVHSSGPWLHLPGHEVRL
SDSEEQLRARLWPLLEAGRYDPPWVRDLARELRQDEAAMRNLLRKLARLGQLQQVVRDLFYPEATLRQLAEI
AVQVRDPAGLIRPAEFRDRLGIGRKRSIQILEHFDRIGLTRRIGNDRRLREGSALAEQLLGGVSP*
AnnotationID:423GeneID:414
AnnotatorUID: diggins
Modification Date/Time: 2005-03-11 06:06:38
Gene Name:selB
Confidence Code:2
GeneProduct:selenocysteine-specific elongation factor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3276
Secondary Functional Category(ies):translation, ribosomal structure and biogenesis
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66123.1|AE016240_8 (AE016775) selenocysteine-specific translation elongation factor
           [Pseudomonas putida KT2440]
          Length = 640

 Score =  783 bits (2022), Expect = 0.0
 Identities = 411/636 (64%), Positives = 493/636 (77%), Gaps = 2/636 (0%)








 
Structural Features:
COG3276, SelB, Selenocysteine-specific translation elongation factor 
[Translation, ribosomal structure and biogenesis]
CD-Length = 447 residues, 100.0% aligned
Score =  480 bits (1238), Expect = 2e-136
Identities = 218/470 (46%), Positives = 274/470 (58%), Gaps = 24/470 (5%)








   
Genomic Context:






   
Comment:


















    ReferenceID:14131




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17973




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35243




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:414_cdd.html


    		




 
URL:

















    ReferenceID:35997




  
Author/Investigator(s):
        Sawers G, Heider J, Zehelein E, Bock A.





  
Title:
        Expression and operon structure of the sel genes of Escherichia coli and identification of a third selenium-containing formate dehydrogenase isoenzyme.




  
PubMed:
        1650339


MedLine:
        




    
Source:
J Bacteriol. 1991 Aug;173(16):4983-93.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1650339

















    ReferenceID:35998




  
Author/Investigator(s):
        Gursinsky T, Jager J, Andreesen JR, Sohling B.





  
Title:
        A selDABC cluster for selenocysteine incorporation in Eubacterium acidaminophilum.




  
PubMed:
        11041351


MedLine:
        




    
Source:
Arch Microbiol. 2000 Sep;174(3):200-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11041351









           
Homologs By Global Alignment 

           

       



     
Gene ID:414
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
700
                           PA4807_tr
                           translation of PA4807
                           641
                           99.53
                           99.68
                           0
                           3251.0
     

     
     
31683
                           gb|AAG08193.1|AE004893_11
                           (AE004893) selenocysteine-specific elongation factor [Pseudomonas aeruginosa PAO1]
                           641
                           99.37
                           99.68
                           0
                           3248.0
     

     
     
31684
                           gb|AAN66123.1|AE016240_8
                           (AE016775) selenocysteine-specific translation elongation factor [Pseudomonas putida KT2440]
                           643
                           63.76
                           76.67
                           5
                           2014.0
     

     
     
31686
                           emb|CAA34637.1|
                           (X16644) unnamed protein product [Escherichia coli]
                           658
                           44.22
                           58.20
                           61
                           1253.0
     

     
     
31687
                           gb|AAC76614.1|
                           (AE000436) selenocysteinyl-tRNA-specific translation factor [Escherichia coli K12]
                           658
                           44.22
                           58.20
                           61
                           1253.0
     

     






      

          
GC ORFID: 43416How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4807Glimmer Score: 

          
Start: 4928768Stop: 4926843

          
Length: 1926


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4807 translation of PA4807Homolog Bit Score 1256.0

          
Other Homologs:  t_PA4807 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGATCGTCGGCACCGCCGGGCACATCGACCACGGCAAGACCTCGCTGCTGCGCGCCCTCACCGGCATCGAG 
GGCGACCGCCGCCCGGCCGAGCGCCAACGCGGGATCACCATCGACCTGGGCTATCTCTACGCCGACCTTGGC 
GACGGCAGCCCCACCGGCTTCATCGACGTGCCCGGTCACGAGCGCTTCGTCCACAACATGCTGGCCGGCGCC 
AGCGGCATCGATTGCGTGCTGCTGGTGGTGGCCGCCGACGACGGCCTGATGCCGCAGACCCGCGAGCACCTG 
GCGATCGTCGAACTGCTCGGTATCCGCCGGGCGCTGGTGGCGCTGACCAAGATCGACCGGGTCGAGCCGCAG 
CGGGTGCAGCAGGTGCGGACCCAGGTCGAACACCTGCTGGCCTCCGGCCCGCTGGCGCGCTCGCCGATCTTC 
CCGCTCTCCAGCAGCACCGGGGAGGGCGTCGCGGCGTTGCGCGAAGCACTCGGCGGGCTCGCCGGCGAAGTC 
CGCGAGCGTAGCCGCGAGGGATACTTCCGCCTGGCGGTGGACCGGGCCTTCAGCGTCGCCGGCAGCGGCATC 
GTGGTCACCGGCACGGCCTTCTCCGGACAGGTCGCGGTGGGCGACGAACTGCTCCTCGGCAACGCCGGACGG 
CCGGTGCGGGTGCGCGGCCTGCACGCGCAGAACCGGCCCGCGCAACAGGCCTGGGCCGGCCAGCGCGTGGCC 
CTGAACATCGCCGGCGAGCGCCTCGCGCTGGAACAGATCCAGCGCGGCGACTGGCTGCTGCAGGCAGCGCTG 
CACGCGCCGACCACGCGGGTCGACATCGACTTCCGCCTGTTGCCCGACGAACTTCGCGACTTCACCCACTGG 
ACCCCGGTGCACCTGCACCTGGGCACCCAGGACGTCACCGGGCGCCTGGCCCTGCTCGAAGGCGAACAACTG 
GAGCCTGGCCACCGCGCCTTCGCCCAACTGGTGCTGAACGCGCCGATCCAGGCCCTGCACGGCGACCGCCTG 
GTGCTGCGCGACCAGTCGGCGCAGCGCACCCTTGGCGGCGGGCGGGTGCTCGACGGCGCCGCGCCGGCGCGC 
AACCGACGAACGCCGGCACGTCTGGCACAGCTCCAGGCGCTGCGCCAGGAAAGCCTGGAGGAGGCGCTGCCG 
ACCTTGCTCGGCGCGGCCGAGAACGGCCTCGACCCGCAACGCCTGGTGCAATCCTTCAACCGCCCGCGCGAG 
CACTGGCGGCTGCCGGAAGAGGTGGTCGAGGTGCAGACCCGCCTCGGCCCCCGGTTGTTCGCCGGGGAGCGC 
TGGTTCGCCCTGGTCGAGCAACTGCTCGCCGGCCTGCGGCGCTTCCACGAAGAGCTACCGGACGAACTCGGT 
CCCGACCGCGACCGCCTGCGTCGCTACGCCCTGCCGCAGCTGGAGCGGCCGGTGTTCATCGCCTTGCTCGAG 
GCCGCCCTGGCCGGCGGCGACGTGCACAGCAGCGGGCCCTGGCTGCACCTGCCCGGCCATGAAGTGCGCCTG 
AGCGATAGCGAGGAGCAACTGCGCGCGCGCCTCTGGCCGCTGCTCGAAGCCGGCCGCTACGACCCGCCCTGG 
GTTCGCGACCTGGCCCGCGAGCTGCGCCAGGACGAAGCCGCCATGCGCAACCTGCTGCGCAAGCTGGCGCGC 
CTGGGCCAGTTGCAGCAGGTGGTGCGCGACCTGTTCTACCCGGAAGCCACCCTGCGTCAATTGGCCGAGATC 
GCCGTGCAGGTCCGCGACCCCGCCGGGCTGATCCGCCCGGCGGAATTCCGCGACCGCCTGGGCATCGGCCGC 
AAGCGCAGCATCCAGATCCTCGAGCACTTCGACCGCATCGGCCTGACCCGCCGCATCGGCAACGACCGTCGC 
CTGCGCGAGGGCTCGGCGCTGGCCGAACAGTTGCTCGGCGGCGTCTCGCCCTGA