ViewGene

Locus Name:
GeneID:
Location:

GeneID:4125
TrivialName: PA14_28660
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_28660
PAO1 Ortholog Locus:PA2743
Sequence Length:534
Protein Length:177
Start: 2469159
Stop: 2469692
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06131.1|AE004702_8 (AE004702) translation initiation factor IF-3 [Pseudomonas aeruginosa PAO1]
Identities = 177/177 (100%), Positives = 177/177 (100%)
Sequence:
ATGAGACAGGATAAGCGAGCTCAACCGAAACCCCCGATCAACGAGAACATCTCGGCTCGTGAGGTACGGTTG
ATTGGAGCTGATGGCCAGCAGGTTGGTGTTGTTTCGATCGATGAGGCGATCCGCCTAGCCGAAGAGGCGAAG
CTGGACCTGGTTGAGATTTCGGCCGACGCGGTGCCTCCTGTCTGCCGCATCATGGACTACGGCAAGCACCTG
TTCGAGAAGAAGAAGCAGGCTGCGGTCGCCAAGAAGAACCAGAAGCAGGCGCAGGTCAAAGAAATCAAGTTT
CGTCCAGGGACGGAAGAAGGGGATTACCAGGTAAAACTACGCAACCTGGTACGTTTCCTTAGTGAAGGGGAC
AAGGCCAAGGTATCCCTGCGATTCCGCGGCCGTGAGATGGCTCACCAGGAGCTGGGGATGGAGCTGTTGAAG
CGGGTCGAAGCCGACCTCGTGGAGTACGGCACCGTCGAGCAGCATCCTAAGCTGGAAGGACGCCAGCTGATG
ATGGTCATCGCTCCCAAGAAGAAAAAGTAA
Translation:
MRQDKRAQPKPPINENISAREVRLIGADGQQVGVVSIDEAIRLAEEAKLDLVEISADAVPPVCRIMDYGKHL
FEKKKQAAVAKKNQKQAQVKEIKFRPGTEEGDYQVKLRNLVRFLSEGDKAKVSLRFRGREMAHQELGMELLK
RVEADLVEYGTVEQHPKLEGRQLMMVIAPKKKK*
AnnotationID:4132GeneID:4125
AnnotatorUID: diggins
Modification Date/Time: 2005-04-05 08:08:31
Gene Name:infC
Confidence Code:2
GeneProduct:translation initiation factor IF-3
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0290,pfam00707
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:
Homology:
>gb|AAN68078.1|AE016439_13 (AE016783) translation initiation factor IF-3 [Pseudomonas putida
           KT2440]
          Length = 177

 Score =  319 bits (818), Expect = 4e-87
 Identities = 157/177 (88%), Positives = 170/177 (96%)

>gb|AAL87031.1|AF332624_2 (AF332624) initiation factor 3 [Azotobacter vinelandii]
          Length = 181

 Score =  319 bits (817), Expect = 5e-87
 Identities = 157/177 (88%), Positives = 170/177 (96%)

>gb|AAO55890.1| (AE016864) translation initiation factor IF-3 [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 177

 Score =  318 bits (816), Expect = 7e-87
 Identities = 157/177 (88%), Positives = 170/177 (96%)

Structural Features:
COG0290, InfC, Translation initiation factor 3 (IF-3) 
[Translation, ribosomal structure and biogenesis]
CD-Length = 176 residues,  95.5% aligned
Score =  224 bits (572), Expect = 3e-60
Identities = 111/168 (66%), Positives = 138/168 (82%)

pfam00707, IF3_C, Translation initiation factor IF-3, C-terminal domain. 
CD-Length = 88 residues, 100.0% aligned
Score =  106 bits (264), Expect = 2e-24
Identities = 50/88 (56%), Positives = 64/88 (72%)
Genomic Context:
Comment:
ReferenceID:8252
Author/Investigator(s): GenNotator
Title:
PubMed:
MedLine:
Source:
Reference Type:BLASTP
Data:
URL:
ReferenceID:25395
Author/Investigator(s): GenNotator
Title: CDD Search
PubMed:
MedLine:
Source:
Reference Type:RPSBLAST
Data:
URL:
ReferenceID:36293
Author/Investigator(s):
Title: CDD Search
PubMed:
MedLine:
Source:
Reference Type:Misc (text/plain)
Data:4125_cdd.html
URL:
ReferenceID:36382
Author/Investigator(s): Kolibachuk D, Rouhbakhsh D, Baumann P.
Title: Aromatic amino acid biosynthesis in Buchnera aphidicola (endosymbiont of aphids): cloning and sequencing of a DNA fragment containing aroH-thrS-infC-rpmI-rplT.
PubMed: 7766160
MedLine:
Source:
Curr Microbiol. 1995 May;30(5):313-6.
Reference Type:Journal
Data:
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7766160
ReferenceID:36386
Author/Investigator(s): Pon CL, Brombach M, Thamm S, Gualerzi CO.
Title: Cloning and characterization of a gene cluster from Bacillus stearothermophilus comprising infC, rpmI and rplT.
PubMed: 2779520
MedLine:
Source:
Mol Gen Genet. 1989 Aug;218(2):355-7.
Reference Type:Journal
Data:
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2779520
ReferenceID:36388
Author/Investigator(s): Liveris D, Schwartz JJ, Geertman R, Schwartz I.
Title: Molecular cloning and sequencing of infC, the gene encoding translation initiation factor IF3, from four enterobacterial species.
PubMed: 8405963
MedLine:
Source:
FEMS Microbiol Lett. 1993 Sep 1;112(2):211-6.
Reference Type:Journal
Data:
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8405963
ReferenceID:36389
Author/Investigator(s): Pramanik A, Wertheimer SJ, Schwartz JJ, Schwartz I.
Title: Expression of Escherichia coli infC: identification of a promoter in an upstream thrS coding sequence.
PubMed: 3536858
MedLine:
Source:
J Bacteriol. 1986 Nov;168(2):746-51.
Reference Type:Journal
Data:
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3536858
Homologs By Global Alignment
Gene ID:4125

Identity:

0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100%
HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score
6380 PA2743_tr translation of PA2743 183 96.72 96.72 6 887.0
174891 gb|AAG06131.1|AE004702_8 (AE004702) translation initiation factor IF-3 [Pseudomonas aeruginosa PAO1] 183 96.72 96.72 6 887.0
174892 gb|AAN68078.1|AE016439_13 (AE016783) translation initiation factor IF-3 [Pseudomonas putida KT2440] 177 88.70 96.04 0 818.0
174894 gb|AAO55890.1| (AE016864) translation initiation factor IF-3 [Pseudomonas syringae pv. tomato str. DC3000] 177 88.70 96.04 0 816.0
174893 gb|AAL87031.1|AF332624_2 (AF332624) initiation factor 3 [Azotobacter vinelandii] 181 86.74 93.92 4 817.0
GC ORFID: 47127How Found: BLASTX
GC_TrimmedSeqID: 69Blast Result ID
Subject Sequence Name: t_PA2743Glimmer Score:
Start: 2484767Stop: 2484234
Length: 534
Start Codon: ATGTruncated Start:
Stop Codon: TAATruncated Stop:
Homolog: t_PA2743 translation of PA2743Homolog Bit Score 356.0
Other Homologs: psyr_15may02_Scaffold1_revised_gene1164 (ORF: BLASTX 318.0), t_PA2743 (ORF: GLIMMER)
GC ORF Sequence
ATGAGACAGGATAAGCGAGCTCAACCGAAACCCCCGATCAACGAGAACATCTCGGCTCGTGAGGTACGGTTG
ATTGGAGCTGATGGCCAGCAGGTTGGTGTTGTTTCGATCGATGAGGCGATCCGCCTAGCCGAAGAGGCGAAG
CTGGACCTGGTTGAGATTTCGGCCGACGCGGTGCCTCCTGTCTGCCGCATCATGGACTACGGCAAGCACCTG
TTCGAGAAGAAGAAGCAGGCTGCGGTCGCCAAGAAGAACCAGAAGCAGGCGCAGGTCAAAGAAATCAAGTTT
CGTCCAGGGACGGAAGAAGGGGATTACCAGGTAAAACTACGCAACCTGGTACGTTTCCTTAGTGAAGGGGAC
AAGGCCAAGGTATCCCTGCGATTCCGCGGCCGTGAGATGGCTCACCAGGAGCTGGGGATGGAGCTGTTGAAG
CGGGTCGAAGCCGACCTCGTGGAGTACGGCACCGTCGAGCAGCATCCTAAGCTGGAAGGACGCCAGCTGATG
ATGGTCATCGCTCCCAAGAAGAAAAAGTAA