ViewGene
Locus Name:
GeneID:
Location:

GeneID:4077
TrivialName: PA14_62990
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62990
PAO1 Ortholog Locus:PA4762
Sequence Length:561
Protein Length:186
Start: 5621690
Stop: 5621130
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08148.1|AE004890_1 (AE004890) heat shock protein GrpE [Pseudomonas aeruginosa PAO1]
Identities = 186/186 (100%), Positives = 186/186 (100%)
Sequence:
ATGGCTGACGAACAGCAGACCCTGGATCAACAGACCCCCGAGCAGCCCACCGGCGCCGCCGAGGACCTGACC
GCCCGCGTGCAGGAACTGGAGGAGCAGCTGGCTGCCGCCCAGGACCAGGCGCTGCGCATGGTCGCGGATCTG
CAGAACGTACGCCGGCGTGCGGAGCAGGATGTCGAGAAGGCGCACAAGTTCGCGCTGGAGAAATTCGCCGGC
GACCTGCTGGCGGTGGTCGACACCCTCGAGCGCGGCCTGGAGATGTCCGATCCCAATGACGAGGCGATCAAG
CCGATGCGCGAAGGGATGGAACTGACCCTGAAGATGTTCGACGACACCCTGCGCCGCTACCAGGTGGAGGCG
CTGAACCCGGAAGGCGAGCCTTTCAATCCCGAGCAGCACCAGGCGATGGCCATGCAGGAAAGCGCTTCGGCC
GAGCCGGGCAGTGTCCTCAAGGTGTTCCAGAAAGGCTACCTGCTCAACGGCCGCCTGCTGCGTCCGGCGATG
GTGGTGGTCAGCAAGGCGCCGGCGGAAACCCCGCCTTCCATCGACGAGCAGGCTTGA
Translation:
MADEQQTLDQQTPEQPTGAAEDLTARVQELEEQLAAAQDQALRMVADLQNVRRRAEQDVEKAHKFALEKFAG
DLLAVVDTLERGLEMSDPNDEAIKPMREGMELTLKMFDDTLRRYQVEALNPEGEPFNPEQHQAMAMQESASA
EPGSVLKVFQKGYLLNGRLLRPAMVVVSKAPAETPPSIDEQA*
AnnotationID:4084GeneID:4077
AnnotatorUID: diggins
Modification Date/Time: 2005-03-09 11:11:19
Gene Name:grpE
Confidence Code:2
GeneProduct:heat shock protein GrpE
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0576,pfam01025
Secondary Functional Category(ies):DNA eplication, recombination, modification and repair
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57954.1| (AE016872) heat shock protein GrpE [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 187

 Score =  273 bits (698), Expect = 4e-73
 Identities = 139/188 (73%), Positives = 164/188 (87%), Gaps = 3/188 (1%)

>gb|AAN70300.1|AE016671_1 (AE016791) heat shock protein GrpE [Pseudomonas putida KT2440]
          Length = 185

 Score =  247 bits (630), Expect = 3e-65
 Identities = 130/186 (69%), Positives = 148/186 (79%), Gaps = 1/186 (0%)

>gb|AAF94016.1| (AE004170) heat shock protein GrpE [Vibrio cholerae]
          Length = 200

 Score =  164 bits (416), Expect = 2e-40
 Identities = 83/151 (54%), Positives = 113/151 (74%)






 
Structural Features:
COG0576, GrpE, Molecular chaperone GrpE (heat shock protein) 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 193 residues,  86.0% aligned
Score =  158 bits (400), Expect = 5e-40
Identities = 71/166 (42%), Positives = 105/166 (63%)

pfam01025, GrpE, GrpE
CD-Length = 167 residues, 100.0% aligned
Score =  142 bits (359), Expect = 3e-35
Identities = 69/168 (41%), Positives = 109/168 (64%), Gaps = 6/168 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8156




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25299




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36561




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4077_cdd.html


    		




 
URL:

















    ReferenceID:36616




  
Author/Investigator(s):
        Amemura-Maekawa J, Watanabe H.





  
Title:
        Cloning and sequencing of the dnaK and grpE genes of Legionella pneumophila.




  
PubMed:
        9332363


MedLine:
        




    
Source:
Gene. 1997 Sep 15;197(1-2):165-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9332363

















    ReferenceID:36617




  
Author/Investigator(s):
        Lipinska B, King J, Ang D, Georgopoulos C.





  
Title:
        Sequence analysis and transcriptional regulation of the Escherichia coli grpE gene, encoding a heat shock protein.




  
PubMed:
        3045760


MedLine:
        




    
Source:
Nucleic Acids Res. 1988 Aug 11;16(15):7545-62.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3045760

















    ReferenceID:36618




  
Author/Investigator(s):
        Wetzstein M, Volker U, Dedio J, Lobau S, Zuber U, Schiesswohl M, Herget C, Hecker M, Schumann W.





  
Title:
        Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis.




  
PubMed:
        1339421


MedLine:
        




    
Source:
J Bacteriol. 1992 May;174(10):3300-10.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1339421

















    ReferenceID:36619




  
Author/Investigator(s):
        Eaton T, Shearman C, Gasson M.





  
Title:
        Cloning and sequence analysis of the dnaK gene region of Lactococcus lactis subsp. lactis.




  
PubMed:
        8126443


MedLine:
        




    
Source:
J Gen Microbiol. 1993 Dec;139 ( Pt 12):3253-64.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8126443









           
Homologs By Global Alignment 

           

       



     
Gene ID:4077
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6320
                           PA4762_tr
                           translation of PA4762
                           186
                           99.99
                           99.99
                           0
                           931.0
     

     
     
173635
                           gb|AAG08148.1|AE004890_1
                           (AE004890) heat shock protein GrpE [Pseudomonas aeruginosa PAO1]
                           186
                           99.99
                           99.99
                           0
                           931.0
     

     
     
173636
                           gb|AAO57954.1|
                           (AE016872) heat shock protein GrpE [Pseudomonas syringae pv. tomato str. DC3000]
                           188
                           73.93
                           87.23
                           3
                           702.0
     

     
     
173637
                           gb|AAN70300.1|AE016671_1
                           (AE016791) heat shock protein GrpE [Pseudomonas putida KT2440]
                           186
                           69.35
                           80.10
                           1
                           632.0
     

     
     
173638
                           emb|CAE15746.1|
                           (BX571870) GrpE protein (HSP-70 cofactor) (heat shock protein B25.3) (HSP24) [Photorhabdus luminescens subsp. laumondii TTO1]
                           206
                           46.60
                           57.28
                           33
                           441.0
     

     






      

          
GC ORFID: 47079How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4762Glimmer Score: 

          
Start: 4885370Stop: 4884810

          
Length: 561


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4762 translation of PA4762Homolog Bit Score 363.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5474 (ORF: BLASTX 273.0), t_PA4762 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTGACGAACAGCAGACCCTGGATCAACAGACCCCCGAGCAGCCCACCGGCGCCGCCGAGGACCTGACC 
GCCCGCGTGCAGGAACTGGAGGAGCAGCTGGCTGCCGCCCAGGACCAGGCGCTGCGCATGGTCGCGGATCTG 
CAGAACGTACGCCGGCGTGCGGAGCAGGATGTCGAGAAGGCGCACAAGTTCGCGCTGGAGAAATTCGCCGGC 
GACCTGCTGGCGGTGGTCGACACCCTCGAGCGCGGCCTGGAGATGTCCGATCCCAATGACGAGGCGATCAAG 
CCGATGCGCGAAGGGATGGAACTGACCCTGAAGATGTTCGACGACACCCTGCGCCGCTACCAGGTGGAGGCG 
CTGAACCCGGAAGGCGAGCCTTTCAATCCCGAGCAGCACCAGGCGATGGCCATGCAGGAAAGCGCTTCGGCC 
GAGCCGGGCAGTGTCCTCAAGGTGTTCCAGAAAGGCTACCTGCTCAACGGCCGCCTGCTGCGTCCGGCGATG 
GTGGTGGTCAGCAAGGCGCCGGCGGAAACCCCGCCTTCCATCGACGAGCAGGCTTGA