ViewGene
Locus Name:
GeneID:
Location:

GeneID:407
TrivialName: PA14_05360
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05360
PAO1 Ortholog Locus:PA0411
Sequence Length:2049
Protein Length:682
Start: 465868
Stop: 467916
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03800.1|AE004478_5 (AE004478) twitching motility protein PilJ [Pseudomonas aeruginosa PAO1]
Identities = 681/682 (99%), Positives = 681/682 (99%)
Sequence:
ATGAAGAAAATCAACGCAGGCAATCTTTTCGCGGGCATGCGCAGCAGCTCGGTGATCGCGGGGCTGTTCATC
GTCCTCATCGTCTCGATCGTGTTGCTGTTCGCCAACTTTGCCTACCTCAACACCCAGTCGAACCATGACAAG
CAGTACATCGGCCATGCCGGCGAACTGCGCGTCCTGTCGCAGCGGATCGCGAAGAACGCCACCGAAGCGGCG
GCGGGCAAGGGCGAGGCGTTCAAGCTCCTGAAGGATGCGCGCAACGACTTCGAGAAACGCTGGAACATCTTG
GTCAACGGCGACGAGAGCACATCCCTGCCGCCCAGCCCCGAAGCGGTGAAGCCGCAGATGGACGTGGTGCAG
CAGGACTGGGACGGTCTGCGCAAGAACGCCGACTCCATCCTCGCCAGCGAACAGACCGTACTGTCCCTGCAC
CAGGTGGCCTCGACCCTCGCCGAAACCATCCCGCAGCTGCAGGTGGAATACGAAGAGGTGGTCGACATCCTG
CTGGAGAACGGTGCCCCGGCCGACCAGGTCGCGGTCGCCCAGCGCCAGTCGCTGCTCGCCGAACGTATCCTC
GGCTCGGTGAACAAGGTGCTGGCCGGCGACGAGAACTCGGTGCAGGCCGCCGACAGCTTCGGCCGCGACGCC
AGCCTGTTCGGCCGCGTACTGAAGGGCATGCAGGAAGGCAACGCGGCGATGAGCATCTCCAAGGTGACCAAC
GCCGAGGCGGTGGATCGCCTGAACGAGATCGCCGAACTCTTCGAGTTCGTCTCCGGCTCGGTGGACGAGATC
CTCGAGACCTCGCCCGACCTGTTCCAGGTCCGGGAAGCGGCGAACAACATCTTCAGCGTTTCGCAAACCCTG
CTGGACAAGGCCTCGCAACTGGCCGACGGCTTCGAGAACCTGGCCGGCGGGCGTAGCATCAACCTGTTCGCC
GGTTACGTCCTCGGCGCCCTGGCCCTGGCCTCGATCATCCTGATCGGCCTGGTGATGGTGCGCGAGACCAAC
CGCCGGCTGGCCGAGACCGCCGAGAAGAACGACCGCAACCAGGCCGCGATCCTGCGTCTGCTCGACGAGATC
GCCGACCTCGCGGACGGCGACCTGACCGTGGCCGCGACCGTGACCGAGGACTTCACCGGCGCCATCGCCGAC
TCGATCAACTACTCCATCGACCAGCTCCGCGAACTGGTGGAGACCATCAACCAGACCGCCGTGCAGGTGGCC
GCAGCGGCCCAGGAAACCCAGTCCACCGCGATGCACCTGGCCGAAGCCTCCGAGCACCAGGCGCAGGAAATC
GCCGGCGCCTCGGCGGCGATCAACGAAATGGCGGTGTCGATCGACCAGGTATCGGCGAACGCCTCGGAATCC
TCGGCGGTAGCGGAACGTTCGGTAGCCATCGCCAACAAGGGCAACGAGGTGGTGCACAACACCATCACCGGC
ATGGACAACATTCGCGAGCAGATCCAGGACACCTCGAAGCGAATCAAGCGCCTCGGCGAGTCGTCCCAGGAG
ATCGGCGACATCGTCAGCCTGATCAACGACATCGCCGACCAGACCAACATCCTCGCCCTGAACGCCGCGATC
CAGGCGTCGATGGCCGGGGACGCGGGCCGCGGCTTCGCCGTGGTAGCGGACGAGGTACAGCGACTGGCGGAA
CGTTCCTCGGCGGCGACCAAGCAGATCGAGGCGCTGGTGAAGACCATCCAGACCGACACCAACGAAGCGGTG
ATCTCGATGGAGCAGACCACCTCCGAGGTGGTCCGCGGCGCGCGCCTGGCCCAGGACGCCGGTGTGGCCCTG
GAGGAGATCGAGAAGGTATCCAAGACCCTCGCGGCACTGATCCAGAACATCTCCAACGCCGCCCGTCAGCAG
GCATCGTCGGCCGGCCACATTTCCAACACCATGAACGTCATTCAGGAGATCACCTCGCAGACCTCCGCCGGT
ACCACCGCCACCGCGCGGAGCATCGGCAACCTGGCGAAGATGGCGAGCGAGATGCGCAACTCGGTATCCGGC
TTCAAACTGCCGGAGGGCGTGGAGCAGGCCTGA
Translation:
MKKINAGNLFAGMRSSSVIAGLFIVLIVSIVLLFANFAYLNTQSNHDKQYIGHAGELRVLSQRIAKNATEAA
AGKGEAFKLLKDARNDFEKRWNILVNGDESTSLPPSPEAVKPQMDVVQQDWDGLRKNADSILASEQTVLSLH
QVASTLAETIPQLQVEYEEVVDILLENGAPADQVAVAQRQSLLAERILGSVNKVLAGDENSVQAADSFGRDA
SLFGRVLKGMQEGNAAMSISKVTNAEAVDRLNEIAELFEFVSGSVDEILETSPDLFQVREAANNIFSVSQTL
LDKASQLADGFENLAGGRSINLFAGYVLGALALASIILIGLVMVRETNRRLAETAEKNDRNQAAILRLLDEI
ADLADGDLTVAATVTEDFTGAIADSINYSIDQLRELVETINQTAVQVAAAAQETQSTAMHLAEASEHQAQEI
AGASAAINEMAVSIDQVSANASESSAVAERSVAIANKGNEVVHNTITGMDNIREQIQDTSKRIKRLGESSQE
IGDIVSLINDIADQTNILALNAAIQASMAGDAGRGFAVVADEVQRLAERSSAATKQIEALVKTIQTDTNEAV
ISMEQTTSEVVRGARLAQDAGVALEEIEKVSKTLAALIQNISNAARQQASSAGHISNTMNVIQEITSQTSAG
TTATARSIGNLAKMASEMRNSVSGFKLPEGVEQA*
AnnotationID:416GeneID:407
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 08:08:25
Gene Name:pilJ
Confidence Code:1
GeneProduct:type IV pili methyl-accepting chemotaxis transducer PilJ
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(10) Chemotaxis
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:smart00283,COG0840,pfam00015
Secondary Functional Category(ies):motility and attachment
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70555.1|AE016698_3 (AE016792) type IV pili methyl-accepting chemotaxis transducer PilJ
           [Pseudomonas putida KT2440]
          Length = 686

 Score =  962 bits (2486), Expect = 0.0
 Identities = 511/667 (76%), Positives = 591/667 (88%)

>gb|AAO58459.1| (AE016874) type IV pilus biogenesis protein PilJ [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 680

 Score =  957 bits (2475), Expect = 0.0
 Identities = 516/665 (77%), Positives = 579/665 (87%)








 
Structural Features:
smart00283, MA, Methyl-accepting chemotaxis-like domains 
(chemotaxis sensory transducer). 
CD-Length = 262 residues, 100.0% aligned
Score =  191 bits (486), Expect = 2e-49
Identities = 113/262 (43%), Positives = 178/262 (67%)

COG0840, Tar, Methyl-accepting chemotaxis protein 
[Cell motility and secretion / Signal transduction mechanisms]
CD-Length = 408 residues,  96.3% aligned
Score =  159 bits (402), Expect = 1e-39
Identities = 124/396 (31%), Positives = 215/396 (54%), Gaps = 4/396 (1%)

pfam00015, MCPsignal, Methyl-accepting chemotaxis protein (MCP) signaling domain. 
CD-Length = 251 residues,  99.6% aligned
Score =  150 bits (379), Expect = 6e-37
Identities = 92/250 (36%), Positives = 165/250 (66%)






   
Genomic Context:






   
Comment:


















    ReferenceID:817




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17959




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33738




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:407_cdd.html


    		




 
URL:

















    ReferenceID:35372




  
Author/Investigator(s):
        Darzins A.





  
Title:
        Mol Microbiol. 1994 Jan;11(1):137-53.




  
PubMed:
        7908398


MedLine:
        




    
Source:
Mol Microbiol. 1994 Jan;11(1):137-53.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7908398

















    ReferenceID:35373




  
Author/Investigator(s):
        Darzins A.





  
Title:
        The Pseudomonas aeruginosa pilK gene encodes a chemotactic methyltransferase (CheR) homologue that is translationally regulated.




  
PubMed:
        7783642


MedLine:
        




    
Source:
Mol Microbiol. 1995 Feb;15(4):703-17.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7783642









           
Homologs By Global Alignment 

           

       



     
Gene ID:407
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
691
                           PA0411_tr
                           translation of PA0411
                           682
                           99.85
                           99.85
                           0
                           3275.0
     

     
     
31545
                           gb|AAG03800.1|AE004478_5
                           (AE004478) twitching motility protein PilJ [Pseudomonas aeruginosa PAO1]
                           682
                           99.85
                           99.85
                           0
                           3275.0
     

     
     
31546
                           gb|AAA25952.1|
                           (L22036) methyl-accepting chemotaxis protein (MCP) homologue [Pseudomonas aeruginosa]
                           682
                           99.85
                           99.85
                           0
                           3275.0
     

     
     
31548
                           gb|AAO58459.1|
                           (AE016874) type IV pilus biogenesis protein PilJ [Pseudomonas syringae pv. tomato str. DC3000]
                           682
                           75.80
                           85.33
                           2
                           2462.0
     

     
     
31547
                           gb|AAN70555.1|AE016698_3
                           (AE016792) type IV pili methyl-accepting chemotaxis transducer PilJ [Pseudomonas putida KT2440]
                           686
                           74.92
                           86.88
                           4
                           2482.0
     

     






      

          
GC ORFID: 43409How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0411Glimmer Score: 

          
Start: 6267210Stop: 6269258

          
Length: 2049


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0411 translation of PA0411Homolog Bit Score 1266.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3353 (ORF: BLASTX 959.0), t_PA0411 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGAAAATCAACGCAGGCAATCTTTTCGCGGGCATGCGCAGCAGCTCGGTGATCGCGGGGCTGTTCATC 
GTCCTCATCGTCTCGATCGTGTTGCTGTTCGCCAACTTTGCCTACCTCAACACCCAGTCGAACCATGACAAG 
CAGTACATCGGCCATGCCGGCGAACTGCGCGTCCTGTCGCAGCGGATCGCGAAGAACGCCACCGAAGCGGCG 
GCGGGCAAGGGCGAGGCGTTCAAGCTCCTGAAGGATGCGCGCAACGACTTCGAGAAACGCTGGAACATCTTG 
GTCAACGGCGACGAGAGCACATCCCTGCCGCCCAGCCCCGAAGCGGTGAAGCCGCAGATGGACGTGGTGCAG 
CAGGACTGGGACGGTCTGCGCAAGAACGCCGACTCCATCCTCGCCAGCGAACAGACCGTACTGTCCCTGCAC 
CAGGTGGCCTCGACCCTCGCCGAAACCATCCCGCAGCTGCAGGTGGAATACGAAGAGGTGGTCGACATCCTG 
CTGGAGAACGGTGCCCCGGCCGACCAGGTCGCGGTCGCCCAGCGCCAGTCGCTGCTCGCCGAACGTATCCTC 
GGCTCGGTGAACAAGGTGCTGGCCGGCGACGAGAACTCGGTGCAGGCCGCCGACAGCTTCGGCCGCGACGCC 
AGCCTGTTCGGCCGCGTACTGAAGGGCATGCAGGAAGGCAACGCGGCGATGAGCATCTCCAAGGTGACCAAC 
GCCGAGGCGGTGGATCGCCTGAACGAGATCGCCGAACTCTTCGAGTTCGTCTCCGGCTCGGTGGACGAGATC 
CTCGAGACCTCGCCCGACCTGTTCCAGGTCCGGGAAGCGGCGAACAACATCTTCAGCGTTTCGCAAACCCTG 
CTGGACAAGGCCTCGCAACTGGCCGACGGCTTCGAGAACCTGGCCGGCGGGCGTAGCATCAACCTGTTCGCC 
GGTTACGTCCTCGGCGCCCTGGCCCTGGCCTCGATCATCCTGATCGGCCTGGTGATGGTGCGCGAGACCAAC 
CGCCGGCTGGCCGAGACCGCCGAGAAGAACGACCGCAACCAGGCCGCGATCCTGCGTCTGCTCGACGAGATC 
GCCGACCTCGCGGACGGCGACCTGACCGTGGCCGCGACCGTGACCGAGGACTTCACCGGCGCCATCGCCGAC 
TCGATCAACTACTCCATCGACCAGCTCCGCGAACTGGTGGAGACCATCAACCAGACCGCCGTGCAGGTGGCC 
GCAGCGGCCCAGGAAACCCAGTCCACCGCGATGCACCTGGCCGAAGCCTCCGAGCACCAGGCGCAGGAAATC 
GCCGGCGCCTCGGCGGCGATCAACGAAATGGCGGTGTCGATCGACCAGGTATCGGCGAACGCCTCGGAATCC 
TCGGCGGTAGCGGAACGTTCGGTAGCCATCGCCAACAAGGGCAACGAGGTGGTGCACAACACCATCACCGGC 
ATGGACAACATTCGCGAGCAGATCCAGGACACCTCGAAGCGAATCAAGCGCCTCGGCGAGTCGTCCCAGGAG 
ATCGGCGACATCGTCAGCCTGATCAACGACATCGCCGACCAGACCAACATCCTCGCCCTGAACGCCGCGATC 
CAGGCGTCGATGGCCGGGGACGCGGGCCGCGGCTTCGCCGTGGTAGCGGACGAGGTACAGCGACTGGCGGAA 
CGTTCCTCGGCGGCGACCAAGCAGATCGAGGCGCTGGTGAAGACCATCCAGACCGACACCAACGAAGCGGTG 
ATCTCGATGGAGCAGACCACCTCCGAGGTGGTCCGCGGCGCGCGCCTGGCCCAGGACGCCGGTGTGGCCCTG 
GAGGAGATCGAGAAGGTATCCAAGACCCTCGCGGCACTGATCCAGAACATCTCCAACGCCGCCCGTCAGCAG 
GCATCGTCGGCCGGCCACATTTCCAACACCATGAACGTCATTCAGGAGATCACCTCGCAGACCTCCGCCGGT 
ACCACCGCCACCGCGCGGAGCATCGGCAACCTGGCGAAGATGGCGAGCGAGATGCGCAACTCGGTATCCGGC 
TTCAAACTGCCGGAGGGCGTGGAGCAGGCCTGA