ViewGene
Locus Name:
GeneID:
Location:

GeneID:4069
TrivialName: PA14_05340
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05340
PAO1 Ortholog Locus:PA0410
Sequence Length:537
Protein Length:178
Start: 465247
Stop: 465783
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03799.1|AE004478_4 (AE004478) twitching motility protein PilI [Pseudomonas aeruginosa PAO1]
Identities = 178/178 (100%), Positives = 178/178 (100%)
Sequence:
ATGTCGGACGTTCAGACCCCCTTCCAGCTCCTCGTCGACATCGATCAGCGCTGCCGACGCCTGGCCGCCGGC
CTGCCGGCCCAGCAGGAGGCGGTGCAGAGCTGGAGCGGGATCGGCTTCCGCATGGGCGGGCGGTTCTTCGTC
GCCCCCATGGGGGAGGTCGGCGAAGTCCTGCACGAACCGCGCTACACCCAGCTGCCGGGCGTGAAGACCTGG
GTCAAGGGGGTGGCCAACGTACGTGGCCGCCTGCTGCCGATCATGGACCTCTGCGGTTTCCTCGGCACCGAA
CTGTCGCCCTTGCGCAAGCAGCGGCGGGTGCTGGTGGTGGAGCACCTGGACGTGTTCGCCGGCCTCATCGTC
GATGAGGTGTTCGGCATGCAGCACTTCCCGGTGGACACCTTCAGCGAACAGCTGCCGCCACTAGAAGCGGCA
CTGCAACCCTTCATTCATGGCGTCTTCCATCGAGAGCAGCCGTGGCTGGTGTTCAGCCCGCATGCGCTGGCG
CAACACCAGGGCTTCCTCGACGTCGCCGTATAA
Translation:
MSDVQTPFQLLVDIDQRCRRLAAGLPAQQEAVQSWSGIGFRMGGRFFVAPMGEVGEVLHEPRYTQLPGVKTW
VKGVANVRGRLLPIMDLCGFLGTELSPLRKQRRVLVVEHLDVFAGLIVDEVFGMQHFPVDTFSEQLPPLEAA
LQPFIHGVFHREQPWLVFSPHALAQHQGFLDVAV*
AnnotationID:4076GeneID:4069
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 08:08:59
Gene Name:pilI
Confidence Code:1
GeneProduct:type IV pili signal transduction protein PilI
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(10) Chemotaxis
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00732,pfam01584,cd00588
Secondary Functional Category(ies):motility and attachment
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58460.1| (AE016874) type IV pilus protein PilI [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 179

 Score =  216 bits (551), Expect = 4e-56
 Identities = 105/178 (58%), Positives = 133/178 (74%), Gaps = 1/178 (0%)

>gb|AAN70556.1|AE016698_4 (AE016792) type IV pili signal transduction protein PilI
           [Pseudomonas putida KT2440]
          Length = 184

 Score =  201 bits (512), Expect = 1e-51
 Identities = 102/174 (58%), Positives = 126/174 (72%), Gaps = 1/174 (0%)








 
Structural Features:
cd00732, CheW, CheW, a small regulator protein, unique to the chemotaxis signalling 
in prokaryotes and archea.
CD-Length = 140 residues,  99.3% aligned
Score =  133 bits (336), Expect = 1e-32
Identities = 40/139 (28%), Positives = 63/139 (45%), Gaps = 1/139 (0%)

pfam01584, CheW, CheW-like domain.
CD-Length = 141 residues,  98.6% aligned
Score =  107 bits (268), Expect = 1e-24
Identities = 41/139 (29%), Positives = 61/139 (43%), Gaps = 2/139 (1%)

cd00588, CheW_like, CheW-like domain.
CD-Length = 136 residues,  99.3% aligned
Score = 98.4 bits (245), Expect = 4e-22
Identities = 40/136 (29%), Positives = 63/136 (46%), Gaps = 4/136 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8140




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25283




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33737




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4069_cdd.html


    		




 
URL:

















    ReferenceID:35374




  
Author/Investigator(s):
        Darzins A.





  
Title:
        Characterization of a Pseudomonas aeruginosa gene cluster involved in pilus biosynthesis and twitching motility: sequence similarity to the chemotaxis proteins of enterics and the gliding bacterium Myxococcus xanthus.




  
PubMed:
        7908398


MedLine:
        




    
Source:
Mol Microbiol. 1994 Jan;11(1):137-53.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7908398









           
Homologs By Global Alignment 

           

       



     
Gene ID:4069
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6313
                           PA0410_tr
                           translation of PA0410
                           178
                           99.99
                           99.99
                           0
                           938.0
     

     
     
173591
                           gb|AAG03799.1|AE004478_4
                           (AE004478) twitching motility protein PilI [Pseudomonas aeruginosa PAO1]
                           178
                           99.99
                           99.99
                           0
                           938.0
     

     
     
173592
                           gb|AAA25951.1|
                           (L22036) frzA homologue [Pseudomonas aeruginosa]
                           178
                           99.99
                           99.99
                           0
                           938.0
     

     
     
173593
                           gb|AAO58460.1|
                           (AE016874) type IV pilus protein PilI [Pseudomonas syringae pv. tomato str. DC3000]
                           179
                           58.65
                           74.30
                           1
                           551.0
     

     
     
173594
                           gb|AAN70556.1|AE016698_4
                           (AE016792) type IV pili signal transduction protein PilI [Pseudomonas putida KT2440]
                           184
                           55.43
                           68.47
                           6
                           508.0
     

     






      

          
GC ORFID: 47071How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0410Glimmer Score: 

          
Start: 6266589Stop: 6267125

          
Length: 537


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA0410 translation of PA0410Homolog Bit Score 365.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3352 (ORF: BLASTX 223.0), t_PA0410 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGGACGTTCAGACCCCCTTCCAGCTCCTCGTCGACATCGATCAGCGCTGCCGACGCCTGGCCGCCGGC 
CTGCCGGCCCAGCAGGAGGCGGTGCAGAGCTGGAGCGGGATCGGCTTCCGCATGGGCGGGCGGTTCTTCGTC 
GCCCCCATGGGGGAGGTCGGCGAAGTCCTGCACGAACCGCGCTACACCCAGCTGCCGGGCGTGAAGACCTGG 
GTCAAGGGGGTGGCCAACGTACGTGGCCGCCTGCTGCCGATCATGGACCTCTGCGGTTTCCTCGGCACCGAA 
CTGTCGCCCTTGCGCAAGCAGCGGCGGGTGCTGGTGGTGGAGCACCTGGACGTGTTCGCCGGCCTCATCGTC 
GATGAGGTGTTCGGCATGCAGCACTTCCCGGTGGACACCTTCAGCGAACAGCTGCCGCCACTAGAAGCGGCA 
CTGCAACCCTTCATTCATGGCGTCTTCCATCGAGAGCAGCCGTGGCTGGTGTTCAGCCCGCATGCGCTGGCG 
CAACACCAGGGCTTCCTCGACGTCGCCGTATAA