ViewGene
Locus Name:
GeneID:
Location:

GeneID:4067
TrivialName: PA14_67500
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_67500
PAO1 Ortholog Locus:PA5111
Sequence Length:531
Protein Length:176
Start: 6026397
Stop: 6025867
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08496.1|AE004924_1 (AE004924) lactoylglutathione lyase [Pseudomonas aeruginosa PAO1]
Identities = 175/176 (99%), Positives = 176/176 (100%)
Sequence:
ATGAGTTTCAACACCGAAGTACAGCCCGGCATCTGCATGGAGCCGGACGCCATCACCCAGGAATACGTGTTC
AACCACACCATGTTGCGGGTCAAGGATCCGAAGCGCTCGCTCGACTTCTACTCGCGGGTGCTCGGCATGCGC
CTGCTGCGTCGCCTGGATTTCGAGGAAGGACGGTTCTCCCTGTATTTCCTCGCCATGACCCGTGGCGAAGAG
GTGCCCGACGCGCTCGACGAACGCCAGCGCTACACCTTCGGACGCCAGTCGGTCCTCGAGCTGACCCACAAC
TGGGGCAGCGAGAGCGACGACAGCCAGTACCACAACGGCAACCAGGACCCGCGCGGGTTCGGCCACATCTGC
TTCTCGGTGCCCGACCTGGTGGCGGCCTGCGAGCGTTTCGAGACGCTCGGGGTGAACTTCGTCAAGCCGCTG
GACCGAGGCATGAAGAACGTGGCGTTCATCAGCGATCCCGACGGCTACTGGGTGGAAATCGTCCAGGCCAGC
CTGAACGGCGAGATGGGACGCGGCTGA
Translation:
MSFNTEVQPGICMEPDAITQEYVFNHTMLRVKDPKRSLDFYSRVLGMRLLRRLDFEEGRFSLYFLAMTRGEE
VPDALDERQRYTFGRQSVLELTHNWGSESDDSQYHNGNQDPRGFGHICFSVPDLVAACERFETLGVNFVKPL
DRGMKNVAFISDPDGYWVEIVQASLNGEMGRG*
AnnotationID:4074GeneID:4067
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 12:12:27
Gene Name:gloA3
Confidence Code:2
GeneProduct:lactoylglutathione lyase
Cell Localization:()
Synonyms:gloA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00903,COG0346
Secondary Functional Category(ies):amino acid transport and metabolism
EC Number:4.4.1.5
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56593.1| (AE016867) lactoylglutathione lyase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 173

 Score =  192 bits (488), Expect = 8e-49
 Identities = 94/160 (58%), Positives = 116/160 (72%), Gaps = 2/160 (1%)

>gb|AAN69360.1|AE016573_5 (AE016788) lactoylglutathione lyase [Pseudomonas putida KT2440]
          Length = 175

 Score =  187 bits (476), Expect = 2e-47
 Identities = 93/160 (58%), Positives = 115/160 (71%), Gaps = 2/160 (1%)

>gb|AAM39891.1| (AE012155) lactoylglutathione lyase [Xanthomonas campestris pv.
           campestris str. ATCC 33913]
          Length = 174

 Score =  183 bits (465), Expect = 4e-46
 Identities = 98/160 (61%), Positives = 116/160 (72%), Gaps = 4/160 (2%)






 
Structural Features:
pfam00903, Glyoxalase, Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily.
CD-Length = 120 residues,  99.2% aligned
Score = 87.1 bits (215), Expect = 1e-18
Identities = 40/143 (27%), Positives = 56/143 (39%), Gaps = 26/143 (18%)

COG0346, GloA, Lactoylglutathione lyase and related lyases 
[Amino acid transport and metabolism]
CD-Length = 138 residues,  98.6% aligned
Score = 74.1 bits (180), Expect = 8e-15
Identities = 36/146 (24%), Positives = 50/146 (34%), Gaps = 13/146 (8%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8136




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25279




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34967




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4067_cdd.html


    		




 
URL:

















    ReferenceID:35805




  
Author/Investigator(s):
        Clugston SL, Daub E, Kinach R, Miedema D, Barnard JF, Honek JF.





  
Title:
        Isolation and sequencing of a gene coding for glyoxalase I activity from Salmonella typhimurium and comparison with other glyoxalase I sequences.




  
PubMed:
        9047352


MedLine:
        




    
Source:
Gene. 1997 Feb 20;186(1):103-11.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9047352









           
Homologs By Global Alignment 

           

       



     
Gene ID:4067
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6311
                           PA5111_tr
                           translation of PA5111
                           176
                           99.43
                           99.99
                           0
                           938.0
     

     
     
173575
                           gb|AAG08496.1|AE004924_1
                           (AE004924) lactoylglutathione lyase [Pseudomonas aeruginosa PAO1]
                           176
                           99.43
                           99.99
                           0
                           938.0
     

     
     
173579
                           gb|AAM39891.1|
                           (AE012155) lactoylglutathione lyase [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           181
                           56.35
                           67.95
                           12
                           463.0
     

     
     
173576
                           gb|AAO56593.1|
                           (AE016867) lactoylglutathione lyase [Pseudomonas syringae pv. tomato str. DC3000]
                           180
                           53.88
                           67.22
                           11
                           483.0
     

     
     
173577
                           gb|AAN69360.1|AE016573_5
                           (AE016788) lactoylglutathione lyase [Pseudomonas putida KT2440]
                           180
                           53.88
                           67.22
                           9
                           479.0
     

     






      

          
GC ORFID: 47069How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5111Glimmer Score: 

          
Start: 5290082Stop: 5289552

          
Length: 531


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5111 translation of PA5111Homolog Bit Score 365.0

          
Other Homologs:  psyr_15may02_Scaffold7_revised_gene7206 (ORF: BLASTX 194.0), t_PA5111 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGTTTCAACACCGAAGTACAGCCCGGCATCTGCATGGAGCCGGACGCCATCACCCAGGAATACGTGTTC 
AACCACACCATGTTGCGGGTCAAGGATCCGAAGCGCTCGCTCGACTTCTACTCGCGGGTGCTCGGCATGCGC 
CTGCTGCGTCGCCTGGATTTCGAGGAAGGACGGTTCTCCCTGTATTTCCTCGCCATGACCCGTGGCGAAGAG 
GTGCCCGACGCGCTCGACGAACGCCAGCGCTACACCTTCGGACGCCAGTCGGTCCTCGAGCTGACCCACAAC 
TGGGGCAGCGAGAGCGACGACAGCCAGTACCACAACGGCAACCAGGACCCGCGCGGGTTCGGCCACATCTGC 
TTCTCGGTGCCCGACCTGGTGGCGGCCTGCGAGCGTTTCGAGACGCTCGGGGTGAACTTCGTCAAGCCGCTG 
GACCGAGGCATGAAGAACGTGGCGTTCATCAGCGATCCCGACGGCTACTGGGTGGAAATCGTCCAGGCCAGC 
CTGAACGGCGAGATGGGACGCGGCTGA