ViewGene
Locus Name:
GeneID:
Location:

GeneID:4066
TrivialName: PA14_66650
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66650
PAO1 Ortholog Locus:PA5043
Sequence Length:597
Protein Length:198
Start: 5951039
Stop: 5950443
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08428.1|AE004917_7 (AE004917) type 4 fimbrial biogenesis protein PilN [Pseudomonas aeruginosa PAO1]
Identities = 188/198 (94%), Positives = 191/198 (96%)
Sequence:
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATT
CTTGGCGGCGTCCTTTTGGTTTCCGGCGCACTGGTGTTTCTTGGTGGCCAATATCTCAACACTGCGATTGAG
CATCAGGGCGCGCGCAACGACTTCCTGCGCAAGGAGATCGTTGTGCTGGATGCGCGGATCAAGGAAATCAGC
GAGTTGAGATCGCGGCGCCAGCAGTTGCTCGAGCGGATGAAGATCATTCAGGACCTGCAGGGCAACCGCCCG
ATCATTGGTCGGGTGTTCGACCAGCTCGTGCGGACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATG
ACCGGCAAGAACATCGCCATCGCCGGCGCAGCCGAGTCCAACAACCGCGTTTCCAATCTCATGCGCAACATG
GACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCGGTGACCCAGGGCGCGGTGGACCAG
GCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAAGGCGAAGCATGGGGTT
GCGCAAGGAGCCAAGAAATGA
Translation:
MARINLLPWREELREQRKQQFLVILGGVLLVSGALVFLGGQYLNTAIEHQGARNDFLRKEIVVLDARIKEIS
ELRSRRQQLLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNM
DASEWLTAPTLNEVKAVTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK*
AnnotationID:4073GeneID:4066
AnnotatorUID: diggins
Modification Date/Time: 2005-03-14 17:05:55
Gene Name:pilN
Confidence Code:1
GeneProduct:type 4 fimbrial biogenesis protein PilN
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam05137,COG3166
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAA93087.1| (L28837) membrane protein [Pseudomonas syringae]
          Length = 199

 Score =  262 bits (670), Expect = 8e-70
 Identities = 133/181 (73%), Positives = 158/181 (87%)

>gb|AAO58558.1| (AE016874) type IV pilus biogenesis protein PilN [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 208

 Score =  259 bits (662), Expect = 7e-69
 Identities = 132/181 (72%), Positives = 157/181 (86%)

>emb|CAD86227.1| (BX321864) putative type 4 fimbrial biogenesis protein PilN
           [Nitrosomonas europaea ATCC 19718]
          Length = 203

 Score =  141 bits (356), Expect = 2e-33
 Identities = 78/185 (42%), Positives = 117/185 (63%), Gaps = 3/185 (1%)






 
Structural Features:
pfam05137, PilN, Fimbrial assembly protein (PilN)
CD-Length = 162 residues, 100.0% aligned
Score =  184 bits (468), Expect = 7e-48
Identities = 93/162 (57%), Positives = 114/162 (70%)

COG3166, PilN, Tfp pilus assembly protein PilN 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 206 residues,  96.1% aligned
Score =  175 bits (446), Expect = 2e-45
Identities = 80/198 (40%), Positives = 113/198 (57%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8134




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25277




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35018




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4066_cdd.html


    		




 
URL:

















    ReferenceID:35846




  
Author/Investigator(s):
        Martin PR, Watson AA, McCaul TF, Mattick JS.





  
Title:
        Characterization of a five-gene cluster required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa.




  
PubMed:
        7565110


MedLine:
        




    
Source:
Mol Microbiol. 1995 May;16(3):497-508.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7565110









           
Homologs By Global Alignment 

           

       



     
Gene ID:4066
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6310
                           PA5043_tr
                           translation of PA5043
                           198
                           94.94
                           96.46
                           0
                           937.0
     

     
     
173566
                           gb|AAG08428.1|AE004917_7
                           (AE004917) type 4 fimbrial biogenesis protein PilN [Pseudomonas aeruginosa PAO1]
                           198
                           94.94
                           96.46
                           0
                           937.0
     

     
     
173567
                           gb|AAA87403.1|
                           (U12892) pilN [Pseudomonas aeruginosa]
                           198
                           94.94
                           96.46
                           0
                           937.0
     

     
     
173568
                           gb|AAA93087.1|
                           (L28837) membrane protein [Pseudomonas syringae]
                           199
                           69.84
                           82.41
                           1
                           671.0
     

     
     
173569
                           gb|AAO58558.1|
                           (AE016874) type IV pilus biogenesis protein PilN [Pseudomonas syringae pv. tomato str. DC3000]
                           209
                           66.50
                           78.46
                           12
                           659.0
     

     






      

          
GC ORFID: 47068How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5043Glimmer Score: 

          
Start: 5214722Stop: 5214126

          
Length: 597


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5043 translation of PA5043Homolog Bit Score 365.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3291 (ORF: BLASTX 260.0), t_PA5043 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATT 
CTTGGCGGCGTCCTTTTGGTTTCCGGCGCACTGGTGTTTCTTGGTGGCCAATATCTCAACACTGCGATTGAG 
CATCAGGGCGCGCGCAACGACTTCCTGCGCAAGGAGATCGTTGTGCTGGATGCGCGGATCAAGGAAATCAGC 
GAGTTGAGATCGCGGCGCCAGCAGTTGCTCGAGCGGATGAAGATCATTCAGGACCTGCAGGGCAACCGCCCG 
ATCATTGGTCGGGTGTTCGACCAGCTCGTGCGGACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATG 
ACCGGCAAGAACATCGCCATCGCCGGCGCAGCCGAGTCCAACAACCGCGTTTCCAATCTCATGCGCAACATG 
GACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCGGTGACCCAGGGCGCGGTGGACCAG 
GCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAAGGCGAAGCATGGGGTT 
GCGCAAGGAGCCAAGAAATGA