ViewGene
Locus Name:
GeneID:
Location:

GeneID:4063
TrivialName: PA14_22450
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_22450
PAO1 Ortholog Locus:PA3227
Sequence Length:564
Protein Length:187
Start: 1954808
Stop: 1954245
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06615.1|AE004745_12 (AE004745) peptidyl-prolyl cis-trans isomerase A [Pseudomonas aeruginosa PAO1]
Identities = 185/187 (98%), Positives = 185/187 (98%)
Sequence:
ATGCTCAAACGTCTCCTGATCGCCACCTGCACCCTGCTCTTCGCCGGTTCCCTGCTGGCCGCCACGCCTGCC
GCCAAGCCGCACGTCCTGATCGCCACCAGCCTCGGCGAGATCGAGGTAGAACTCGATCCGACCAAGGCGCCG
ATCAGCGTGAAGAACTTCCTCGAGTACGTCGACAGCGGCTACTACGACGGCACCCTGTTCCACCGCGTGATC
CCGGGCTTCATGGTGCAGACCGGCGGCTTCAGCGCCGGCATGCAGGAGAAGAAGACCCGCGCACCGATCAAG
AACGAGGCCGACAACGGCCTGCTCAACGAGCGCGGCACCCTCGCCATGGCCCGCACCGGCGTGGTCGATTCG
GCCACCAGCCAGTTCTTCATCAACCTGACCGACAACGACTTCCTCAACCATGGTGCCCGCGACTTCGGCTAT
GCGGTGTTCGGCAAGGTGGTGCGTGGCATGGGCGTGGTCGACCAGATCGCCAAGGTTCCCACCACCCGCCGC
AACGGCTTCGCCGACGTGCCGAGCGACGACGTGGTGATCCTGTCCGCCAAACGCCTCTGA
Translation:
MLKRLLIATCTLLFAGSLLAATPAAKPHVLIATSLGEIEVELDPTKAPISVKNFLEYVDSGYYDGTLFHRVI
PGFMVQTGGFSAGMQEKKTRAPIKNEADNGLLNERGTLAMARTGVVDSATSQFFINLTDNDFLNHGARDFGY
AVFGKVVRGMGVVDQIAKVPTTRRNGFADVPSDDVVILSAKRL*
AnnotationID:4070GeneID:4063
AnnotatorUID: diggins
Modification Date/Time: 2005-04-14 13:01:46
Gene Name:ppiA
Confidence Code:2
GeneProduct:peptidyl-prolyl cis-trans isomerase A
Cell Localization:()
Synonyms:cypH
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00160,COG0652
Secondary Functional Category(ies):chaperones and heat shock protein
EC Number:5.2.1.8
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70114.1|AE016651_4 (AE016791) peptidyl-prolyl cis-trans isomerase A [Pseudomonas
           putida KT2440]
          Length = 185

 Score =  229 bits (585), Expect = 5e-60
 Identities = 117/187 (62%), Positives = 143/187 (76%), Gaps = 3/187 (1%)

>gb|AAP19333.1| (AE016992) peptidyl-prolyl cis-trans isomerase A (rotamase A)
           [Shigella flexneri 2a str. 2457T]
          Length = 193

 Score =  225 bits (574), Expect = 9e-59
 Identities = 117/192 (60%), Positives = 141/192 (73%), Gaps = 5/192 (2%)

>gb|AAN82576.1|AE016767_336 (AE016767) Peptidyl-prolyl cis-trans isomerase A precursor
           [Escherichia coli CFT073]
          Length = 190

 Score =  225 bits (573), Expect = 1e-58
 Identities = 117/189 (61%), Positives = 140/189 (74%), Gaps = 2/189 (1%)








 
Structural Features:
pfam00160, Pro_isomerase, Cyclophilin type peptidyl-prolyl 
cis-trans isomerase.
CD-Length = 161 residues,  92.5% aligned
Score =  141 bits (357), Expect = 5e-35
Identities = 56/159 (35%), Positives = 70/159 (44%), Gaps = 16/159 (10%)

COG0652, PpiB, Peptidyl-prolyl cis-trans isomerase (rotamase) 
- cyclophilin family 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 158 residues,  99.4% aligned
Score =  195 bits (498), Expect = 2e-51
Identities = 79/162 (48%), Positives = 100/162 (61%), Gaps = 7/162 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8128




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25271




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32314




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4063_cdd.html


    		




 
URL:

















    ReferenceID:33980




  
Author/Investigator(s):
        Liao X, Charlebois I, Ouellet C, Morency MJ, Dewar K, Lightfoot J, Foster J, Siehnel R, Schweizer H, Lam JS, Hancock RE, Levesque RC.





  
Title:
        Physical mapping of 32 genetic markers on the Pseudomonas aeruginosa PAO1 chromosome.




  
PubMed:
        8581173


MedLine:
        




    
Source:
Microbiology. 1996 Jan;142 ( Pt 1):79-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8581173









           
Homologs By Global Alignment 

           

       



     
Gene ID:4063
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6306
                           PA3227_tr
                           translation of PA3227
                           187
                           98.93
                           98.93
                           0
                           937.0
     

     
     
173477
                           gb|AAG06615.1|AE004745_12
                           (AE004745) peptidyl-prolyl cis-trans isomerase A [Pseudomonas aeruginosa PAO1]
                           187
                           98.93
                           98.93
                           0
                           937.0
     

     
     
173478
                           emb|CAA58868.1|
                           (X84050) peptidylprolyl cis-trans isomerase a [Pseudomonas aeruginosa]
                           187
                           79.14
                           79.67
                           33
                           762.0
     

     
     
173479
                           gb|AAN70114.1|AE016651_4
                           (AE016791) peptidyl-prolyl cis-trans isomerase A [Pseudomonas putida KT2440]
                           188
                           62.23
                           76.06
                           4
                           589.0
     

     
     
173489
                           gb|AAA23451.1|
                           (M55429) peptidyl-prolyl cis-trans isomerase a [Escherichia coli]
                           190
                           61.57
                           73.68
                           3
                           575.0
     

     






      

          
GC ORFID: 47065How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3227Glimmer Score: 

          
Start: 2999123Stop: 2999686

          
Length: 564


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3227 translation of PA3227Homolog Bit Score 365.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6053 (ORF: BLASTX 229.0), t_PA1793 (ORF: BLASTX 177.0), psyr_15may02_Scaffold1_revised_gene653 (ORF: BLASTX 167.0), t_PA3227 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTCAAACGTCTCCTGATCGCCACCTGCACCCTGCTCTTCGCCGGTTCCCTGCTGGCCGCCACGCCTGCC 
GCCAAGCCGCACGTCCTGATCGCCACCAGCCTCGGCGAGATCGAGGTAGAACTCGATCCGACCAAGGCGCCG 
ATCAGCGTGAAGAACTTCCTCGAGTACGTCGACAGCGGCTACTACGACGGCACCCTGTTCCACCGCGTGATC 
CCGGGCTTCATGGTGCAGACCGGCGGCTTCAGCGCCGGCATGCAGGAGAAGAAGACCCGCGCACCGATCAAG 
AACGAGGCCGACAACGGCCTGCTCAACGAGCGCGGCACCCTCGCCATGGCCCGCACCGGCGTGGTCGATTCG 
GCCACCAGCCAGTTCTTCATCAACCTGACCGACAACGACTTCCTCAACCATGGTGCCCGCGACTTCGGCTAT 
GCGGTGTTCGGCAAGGTGGTGCGTGGCATGGGCGTGGTCGACCAGATCGCCAAGGTTCCCACCACCCGCCGC 
AACGGCTTCGCCGACGTGCCGAGCGACGACGTGGTGATCCTGTCCGCCAAACGCCTCTGA