ViewGene
Locus Name:
GeneID:
Location:

GeneID:4060
TrivialName: PA14_30670
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_30670
PAO1 Ortholog Locus:PA2584
Sequence Length:561
Protein Length:186
Start: 2658009
Stop: 2658569
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05972.1|AE004687_1 (AE004687) CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 186/186 (100%), Positives = 186/186 (100%)
Sequence:
ATGAATATCCCCAATCTGCTCACGTTACTGCGCGTCCTGCTCATTCCGATCTTCATTCTGCTGTTCTATCTG
CCGACGTCCTGGAGCTACCTGACCGCCAGTGCCGTGTTCGCCCTGGCCGCCGCCACCGACTGGCTGGACGGC
TACCTCGCCCGGCGCCTCGGCCAGAGCACGCCGTTCGGCGCCTTCCTCGACCCGGTCGCGGACAAGCTGATG
GTGGCGGTTGCGCTGGTCCTGCTGGTGGAAGAGCACGCCAACCTGTGGCTGACCCTGCCGGCGGTGATCATC
ATCGGCCGCGAGATCGTGGTTTCCGCCTTGCGCGAGTGGATGGCCGAACTGGGGGCGAGGGCTCAGGTGGCG
GTGTCCAACCTGGGCAAGTGGAAGACCGCGGCGCAGATGCTGGCGCTGGTCATTCTCCTGGCGAATCCGCCG
CTGATGACCTTCTGGGTGATTCTCGGCTACGTGCTGCTGATCATCTCTGCGGCGCTGACCCTGTGGTCCATG
GTGCACTACCTGCTGGCCGCGTGGCCGCATCTGTCGACCACCCCGAAAGAGAAATAA
Translation:
MNIPNLLTLLRVLLIPIFILLFYLPTSWSYLTASAVFALAAATDWLDGYLARRLGQSTPFGAFLDPVADKLM
VAVALVLLVEEHANLWLTLPAVIIIGREIVVSALREWMAELGARAQVAVSNLGKWKTAAQMLALVILLANPP
LMTFWVILGYVLLIISAALTLWSMVHYLLAAWPHLSTTPKEK*
AnnotationID:4067GeneID:4060
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 10:10:24
Gene Name:pgsA
Confidence Code:2
GeneProduct:CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:phosphatidylglycerophosphate synthase
Functional Category Confidence Code:5
COGs:COG0558,pfam01066
Secondary Functional Category(ies):
EC Number:2.7.8.5
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56512.1| (AE016866) CDP-diacylglycerol--glycerol-3-phosphate
           3-phosphatidyltransferase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 188

 Score =  318 bits (816), Expect = 8e-87
 Identities = 155/186 (83%), Positives = 170/186 (91%)

>gb|AAA98757.1| (L29642) phosphatidylglycerophosphate synthase [Pseudomonas
           fluorescens]
          Length = 195

 Score =  282 bits (722), Expect = 6e-76
 Identities = 143/187 (76%), Positives = 157/187 (83%), Gaps = 1/187 (0%)

>gb|AAN54914.1|AE015629_8 (AE015629) CDP-diacylglycerol--glycerol-3-phosphate
           3-phosphatidyltransferase [Shewanella oneidensis MR-1]
          Length = 182

 Score =  209 bits (533), Expect = 5e-54
 Identities = 110/180 (61%), Positives = 139/180 (77%), Gaps = 4/180 (2%)








 
Structural Features:
COG0558, PgsA, Phosphatidylglycerophosphate synthase [Lipid metabolism]
CD-Length = 192 residues,  93.2% aligned
Score =  115 bits (288), Expect = 4e-27
Identities = 92/181 (50%), Positives = 112/181 (61%), Gaps = 2/181 (1%)

pfam01066, CDP-OH_P_transf, CDP-alcohol phosphatidyltransferase.
CD-Length = 145 residues,  99.3% aligned
Score = 77.0 bits (189), Expect = 1e-15
Identities = 55/144 (38%), Positives = 79/144 (54%), Gaps = 4/144 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8122




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25265




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33155




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4060_cdd.html


    		




 
URL:

















    ReferenceID:34533




  
Author/Investigator(s):
        Ashiuchi M, Soda K, Misono H.





  
Title:
        A poly-gamma-glutamate synthetic system of Bacillus subtilis IFO 3336: gene cloning and biochemical analysis of poly-gamma-glutamate produced by Escherichia coli clone cells.




  
PubMed:
        10486244


MedLine:
        




    
Source:
Biochem Biophys Res Commun. 1999 Sep 16;263(1):6-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10486244

















    ReferenceID:34534




  
Author/Investigator(s):
        Dryden SC, Dowhan W.





  
Title:
        Isolation and expression of the Rhodobacter sphaeroides gene (pgsA) encoding phosphatidylglycerophosphate synthase.




  
PubMed:
        8576035


MedLine:
        




    
Source:
J Bacteriol. 1996 Feb;178(4):1030-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8576035

















    ReferenceID:34535




  
Author/Investigator(s):
        Gopalakrishnan AS, Chen YC, Temkin M, Dowhan W.





  
Title:
        Structure and expression of the gene locus encoding the phosphatidylglycerophosphate synthase of Escherichia coli.




  
PubMed:
        3003065


MedLine:
        




    
Source:
J Biol Chem. 1986 Jan 25;261(3):1329-38.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3003065









           
Homologs By Global Alignment 

           

       



     
Gene ID:4060
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6303
                           PA2584_tr
                           translation of PA2584
                           186
                           99.99
                           99.99
                           0
                           938.0
     

     
     
173361
                           gb|AAG05972.1|AE004687_1
                           (AE004687) CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase [Pseudomonas aeruginosa PAO1]
                           186
                           99.99
                           99.99
                           0
                           938.0
     

     
     
173364
                           dbj|BAC55221.1|
                           (AB088753) CDP-diacylglycerol-glycerol-3-phosphate [Pseudomonas stutzeri]
                           186
                           85.48
                           89.24
                           2
                           803.0
     

     
     
173363
                           gb|AAN69681.1|AE016604_3
                           (AE016789) CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase [Pseudomonas putida KT2440]
                           186
                           83.87
                           91.39
                           0
                           811.0
     

     
     
173362
                           gb|AAO56512.1|
                           (AE016866) CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           188
                           82.44
                           90.42
                           2
                           816.0
     

     






      

          
GC ORFID: 47062How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2584Glimmer Score: 

          
Start: 2295917Stop: 2295357

          
Length: 561


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2584 translation of PA2584Homolog Bit Score 365.0

          
Other Homologs:  psyr_15may02_Scaffold16_revised_gene2707 (ORF: BLASTX 318.0), t_PA2584 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAATATCCCCAATCTGCTCACGTTACTGCGCGTCCTGCTCATTCCGATCTTCATTCTGCTGTTCTATCTG 
CCGACGTCCTGGAGCTACCTGACCGCCAGTGCCGTGTTCGCCCTGGCCGCCGCCACCGACTGGCTGGACGGC 
TACCTCGCCCGGCGCCTCGGCCAGAGCACGCCGTTCGGCGCCTTCCTCGACCCGGTCGCGGACAAGCTGATG 
GTGGCGGTTGCGCTGGTCCTGCTGGTGGAAGAGCACGCCAACCTGTGGCTGACCCTGCCGGCGGTGATCATC 
ATCGGCCGCGAGATCGTGGTTTCCGCCTTGCGCGAGTGGATGGCCGAACTGGGGGCGAGGGCTCAGGTGGCG 
GTGTCCAACCTGGGCAAGTGGAAGACCGCGGCGCAGATGCTGGCGCTGGTCATTCTCCTGGCGAATCCGCCG 
CTGATGACCTTCTGGGTGATTCTCGGCTACGTGCTGCTGATCATCTCTGCGGCGCTGACCCTGTGGTCCATG 
GTGCACTACCTGCTGGCCGCGTGGCCGCATCTGTCGACCACCCCGAAAGAGAAATAA