ViewGene
Locus Name:
GeneID:
Location:

GeneID:4034
TrivialName: PA14_45300
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45300
PAO1 Ortholog Locus:PA1481
Sequence Length:543
Protein Length:180
Start: 4037750
Stop: 4037208
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04870.1|AE004577_5 (AE004577) cytochrome C biogenesis protein CcmG [Pseudomonas aeruginosa PAO1]
Identities = 180/180 (100%), Positives = 180/180 (100%)
Sequence:
TTGAAACGAGCGATTCTGCTGCTGCCGCTGGGGATCTTCCTGATCGTCGCGGTGTTCCTCTTCCGCGGCCTC
TGGCTGGACCCCAGCGAGCTGCCCTCGGCGCTGATCGGCAAGCCGTTCCCGGCCTTCGACCTGCCCAGCGTG
CAGGACCCGGCCCGGCGCCTGACCGAGGCCGACCTCAAGGGCAAGCCGGCGCTGGTCAACGTCTGGGGCACC
TGGTGCCCGTCGTGCCGGGTCGAGCACCCGGAACTGACCCGGCTGGCCGAGCAGGGCGTGGTGATCTACGGG
ATCAACTACAAGGACGACAACGCCGCCGCCATCAAGTGGCTGAACGAGCTGCACAATCCCTACCTGCTGAGC
ATCAGCGACGCCGACGGCACCCTGGGCCTGGACCTGGGCGTGTACGGCGCGCCGGAAACCTACCTGATCGAC
AAGCAGGGCATCATCCGCCACAAGATCGTCGGGGTGGTCGACCAGAAAGTCTGGCGCGAGCAACTGGCGCCG
CTGTACCAGCAACTGCTGGACGAGCCGGAGGCCCGATGA
Translation:
MKRAILLLPLGIFLIVAVFLFRGLWLDPSELPSALIGKPFPAFDLPSVQDPARRLTEADLKGKPALVNVWGT
WCPSCRVEHPELTRLAEQGVVIYGINYKDDNAAAIKWLNELHNPYLLSISDADGTLGLDLGVYGAPETYLID
KQGIIRHKIVGVVDQKVWREQLAPLYQQLLDEPEAR*
AnnotationID:4041GeneID:4034
AnnotatorUID: diggins
Modification Date/Time: 2005-02-24 17:05:36
Gene Name:ccmG
Confidence Code:2
GeneProduct:cytochrome C biogenesis protein CcmG
Cell Localization:()
Synonyms:dsbE
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:thiol:disulfide interchange protein DsbE
Functional Category Confidence Code:5
COGs:COG0526
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAA88019.1| (Z47979) periplasmic or inner membrane associated protein
           [Pseudomonas fluorescens]
          Length = 178

 Score =  266 bits (680), Expect = 4e-71
 Identities = 126/176 (71%), Positives = 149/176 (84%), Gaps = 1/176 (0%)

>gb|AAN69900.1|AE016629_2 (AE016790) thiol:disulfide interchange protein DsbE [Pseudomonas
           putida KT2440]
          Length = 178

 Score =  263 bits (673), Expect = 3e-70
 Identities = 125/176 (71%), Positives = 148/176 (84%), Gaps = 1/176 (0%)

>gb|AAN53352.1|AE015474_15 (AE015474) thiol:disulfide interchange protein DsbE [Shewanella
           oneidensis MR-1]
          Length = 184

 Score =  196 bits (498), Expect = 6e-50
 Identities = 93/177 (52%), Positives = 125/177 (70%), Gaps = 1/177 (0%)








 
Structural Features:
COG0526, TrxA, Thiol-disulfide isomerase and thioredoxins 
[Posttranslational modification, protein turnover, chaperones / 
Energy production and conversion]
CD-Length = 127 residues,  99.2% aligned
Score = 62.6 bits (150), Expect = 2e-11
Identities = 37/128 (28%), Positives = 55/128 (42%), Gaps = 6/128 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14992




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25213




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37349




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4034_cdd.html


    		




 
URL:

















    ReferenceID:37949




  
Author/Investigator(s):
        Tanapongpipat S, Reid E, Cole JA, Crooke H.





  
Title:
        Transcriptional control and essential roles of the Escherichia coli ccm gene products in formate-dependent nitrite reduction and cytochrome c synthesis.




  
PubMed:
        9716493


MedLine:
        




    
Source:
Biochem J. 1998 Sep 1;334 ( Pt 2):355-65.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9716493

















    ReferenceID:37953




  
Author/Investigator(s):
        Thony-Meyer L, Fischer F, Kunzler P, Ritz D, Hennecke H.





  
Title:
        Escherichia coli genes required for cytochrome c maturation.




  
PubMed:
        7635817


MedLine:
        




    
Source:
J Bacteriol. 1995 Aug;177(15):4321-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7635817

















    ReferenceID:37956




  
Author/Investigator(s):
        Fabianek RA, Hennecke H, Thony-Meyer L.





  
Title:
        The active-site cysteines of the periplasmic thioredoxin-like protein CcmG of Escherichia coli are important but not essential for cytochrome c maturation in vivo.




  
PubMed:
        9537397


MedLine:
        




    
Source:
J Bacteriol. 1998 Apr;180(7):1947-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=98196743&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:4034
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6274
                           PA1481_tr
                           translation of PA1481
                           180
                           99.99
                           99.99
                           0
                           947.0
     

     
     
172970
                           gb|AAG04870.1|AE004577_5
                           (AE004577) cytochrome C biogenesis protein CcmG [Pseudomonas aeruginosa PAO1]
                           180
                           99.44
                           99.99
                           0
                           945.0
     

     
     
172971
                           emb|CAA88019.1|
                           (Z47979) periplasmic or inner membrane associated protein [Pseudomonas fluorescens]
                           180
                           69.44
                           83.33
                           2
                           678.0
     

     
     
172972
                           gb|AAN69900.1|AE016629_2
                           (AE016790) thiol:disulfide interchange protein DsbE [Pseudomonas putida KT2440]
                           180
                           68.88
                           82.77
                           2
                           671.0
     

     
     
172974
                           gb|AAO57100.1|
                           (AE016869) thiol:disulfide interchange protein DsbE [Pseudomonas syringae pv. tomato str. DC3000]
                           180
                           68.33
                           82.22
                           2
                           650.0
     

     






      

          
GC ORFID: 47036How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1481Glimmer Score: 

          
Start: 916159Stop: 916701

          
Length: 543


          
Start Codon: TTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1481 translation of PA1481Homolog Bit Score 369.0

          
Other Homologs:  psyr_15may02_Scaffold13_revised_gene2231 (ORF: BLASTX 262.0), t_PA1481 (ORF: GLIMMER)

          
GC ORF Sequence

           
TTGAAACGAGCGATTCTGCTGCTGCCGCTGGGGATCTTCCTGATCGTCGCGGTGTTCCTCTTCCGCGGCCTC 
TGGCTGGACCCCAGCGAGCTGCCCTCGGCGCTGATCGGCAAGCCGTTCCCGGCCTTCGACCTGCCCAGCGTG 
CAGGACCCGGCCCGGCGCCTGACCGAGGCCGACCTCAAGGGCAAGCCGGCGCTGGTCAACGTCTGGGGCACC 
TGGTGCCCGTCGTGCCGGGTCGAGCACCCGGAACTGACCCGGCTGGCCGAGCAGGGCGTGGTGATCTACGGG 
ATCAACTACAAGGACGACAACGCCGCCGCCATCAAGTGGCTGAACGAGCTGCACAATCCCTACCTGCTGAGC 
ATCAGCGACGCCGACGGCACCCTGGGCCTGGACCTGGGCGTGTACGGCGCGCCGGAAACCTACCTGATCGAC 
AAGCAGGGCATCATCCGCCACAAGATCGTCGGGGTGGTCGACCAGAAAGTCTGGCGCGAGCAACTGGCGCCG 
CTGTACCAGCAACTGCTGGACGAGCCGGAGGCCCGATGA