ViewGene
Locus Name:
GeneID:
Location:

GeneID:403
TrivialName: PA14_31010
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_31010
PAO1 Ortholog Locus:
Sequence Length:3138
Protein Length:1045
Start: 2697041
Stop: 2693904
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAK24361.1| (AE005908) AcrB/AcrD/AcrF family protein [Caulobacter crescentus CB15]
Identities = 721/1046 (68%), Positives = 859/1046 (82%), Gaps = 7/1046 (0%)
Sequence:
ATGCTCGAACGTATCATTCGCGCGTCCATCGCGCATCGCTGGCTGGTGCTTCTACTGGTGCTCGCCCTGTCC
GGGCTGGGGATCTGGAACTACAGCCGCTTGCCGATCGACGCGATCCCGGACATCACCAATGTCCAAGTGCAG
ATCAATACCGAGGCACCGGGCTACTCGCCAATGGAGGCAGAGCAGCGCGTCACCTTCCCGGTTGAGACGGCA
TTGGCTGGCCTTGCTCGGCTGGACTACACACGTTCGATTTCCCGCTACGGTCTGTCGCAGGTCACGGCGGTC
TTTGAGGACGGCACTGACATCTACTTTGCTCGCCAGCAGGTCGCCGAACGCTTGCAGCAGGCATCCTCGCAG
CTTCCGACGGGCTTGAATCCTTCCCTCGGGCCGGTGGCAACCGGCCTCGGCGAGATATTCATGTACACCTTG
GAACCCGAGAAGGGCTACGAGGAAGTATGGACGCCAACGGCGCTGCGCACGTTGCAGGACTGGTCAGTGCGT
CCGCAGCTTCGCAACCTGAAAGGCGTCACCGAGGTCAACACCATCGGCGGCTACGTGCGCCAATTCCACATC
ACGCCCGATCCGGTCAAGTTGCTGGCCTACGGGTTGACGATGAACGACGTGCTGAACTCCGTCGCGCGCAAC
AACGCGAACGTCGGTGCCGGGTACATCGAGCGTCATGGCGAACAGTATCTGATCCGCATTCCCGGCCAAGTG
GGCGACATAGAGCGGCTGCGGAAGATTGTCGTGGCAACGCGCGATGGCCTTCCGCTCCGAATGGGCGATGTG
GCGGAAGTGCTGGAAGGCAGCGAATTGCGAACCGGCGCTGCAACGAAAGACGGCAAGGAGGTCGTGTTGGGT
ACGGCCTTCATGCTGATTGGCGAGAACAGCCGAGCGGTTGCGCAACGCACGGTGGAAAAGCTCGCTGAAATC
GACGCGACACTTCCCGAGGGAGTCCGCGCGCATGCGGTTTACGACCGCACGCAGCTCGTTGATCGCACCATC
GCCACCGTGCAGAAGAATCTTCTGGAAGGCGCATTGCTGGTGATCGCAGTGCTTTTCCTGCTGCTGGGCAAC
ATGCGGGCAGCTCTGATAACTGCTACCGTGATTCCGCTTGCCATGCTGCTGACGATTACCGGCATGGTGCAA
AACCGTATCTCGGCCAATCTGATGAGCCTGGGCGCGCTGGACTTCGGCCTGATCGTCGATGGCGCGGTCATC
ATCGTGGAGAACTGCTTGCGCAGGTTCAGCGAGCGACAGCACCAGCTCGGTCGCCTGCTGACCCGCGATGAG
CGTTTCTCGCTGGTTTCGAGTGCCAGTGCGGAAGTCATCAAGCCCGCCTTGTTCGGGCTGTTCATCATCACG
GCTGTTTACCTTCCGATCTTCGCGTTGTCGGGCGTGGAAGGAAAGATGTTCCATCCGATGGCCCTGACCGTC
GTCATCGCCTTGACCGGCGCGATGGTGTTGTCGCTGACCTTCGTGCCCGCTGCGGTCGCCCTGTTTGTCACC
GGCAAGGTTTCCGAGAAGGAAACCAAGGTGATGCGCGGCATCAGCCGCTTCTATGCACCGCTTCTCAAGCAG
GCGATTAAGTTGAGAGTCGCCATCGTCGCGGCAGCGGCAGTGCTGGTGGTGCTGTCCGGCCTGCTTGCAGCA
CGCTTGGGAACCGAGTTCATCCCGAACCTGGATGAAGGCGACATCGCCCTGCACGCGATGCGTATCCCCGGA
ACGAGTCTGACGCAGGCGATTGGCATGCAACGCCAACTGGAAGCCCGGATCAAGGAGTTTCCCGAGGTGGAG
GAAGTCGTCGCCAAGATCGGCACCGCAGAGGTTGCGACCGACCCCATGCCGCCTTCGGTCGCTGACACCTTC
ATCATGCTGAAGGATCGCAGCGAATGGCCCGATCCACGCAAGCCGAGGGCCGTACTGCTCGCCGAACTGGAG
AAGGCGGTGAGCGCCATTCCCGGCAACAACTACGAGTTCACTCAACCCGTGCAGATGCGCATGAATGAGTTG
ATTGCAGGTGTTCGTGCCGAGGTGGCAATCAAGGTGTACGGCGACGACATGGAGCAACTGGCAGAAATCGGC
TCGAAGATCGAAGCCGTGGCCGAGAGTATCGACGGGTCTGCCGATGTGGCGCTTGAGCAGATTACGGGCCTG
CCGCTGATGACGATCACGCCGAATGTGGATGCACTGGCTCGGTACGGCCTGGCGATCGACGACATACAGCAG
ACGGTGGCGATCGCGCTAGGCGGTGCCGTCGCTGGCCAGGTGTTTGAGGGAGATCGCCGATTCGACATTGTG
GTTCGGCTACCCGAAGCGCAACGCCAAGACCCGAAGGCACTGGCGACACTGCCCATTCCGATTCAAGCCGGA
ACGGTTGCCGGGCTAGGCCAAGCAGCCTATGTGCCACTCGGCCAGCTTGCGTCCATCGAAGTAGCGCCCGGC
CCCAATCAGATCAGCCGCGAGAACGGCAAACGCCGGATCGTTATCACCAGCAACGTGCGCGGGCGCGATCTA
GGTTCGTTCGTGGAAGAACTACGTGAGCGGGTGAGCCGTGAAATCACACTGCCCGAAGGTTATTGGGTCAGC
TACGGCGGCACGTTCGAGCAACTTATCTCGGCCAGCCAACGCCTGTCCGTCGTCGTGCCGGCCGTGCTGGTT
CTGATTTTCGGCCTGCTGTTCATGGCCTTCGGCTCGGGCCGGGATGCAATGGTCGTCTTTAGCGGCGTTCCG
CTGGCGCTAACGGGTGGTGTAGCCGCATTGTGGCTACGCGATATTCCGCTCTCGATTTCAGCAGGTGTCGGC
TTCATTGCGCTGTCCGGCGTGGCTGTGCTCAACGGACTGGTGATGGTGAGCTTCATTCGCAAGCTGCATCAC
GACGGCGCACCGATTCAGGACGCCATCGTGAATGGCGCGATGACGCGACTGCGCCCAGTGCTGATGACCGCG
CTGGTTGCAAGCCTTGGTTTCGTACCGATGGCCATCAATGTCGGAACAGGCGCGGAAGTGCAAAGACCACTC
GCCACTGTCGTCATCGGCGGCATCGTTTCCTCGACGCTGCTGACGCTGCTGGTTCTACCTGCGCTGTACCGG
CTGGTCTATCGCCACAAAAACACCGAGAGGGTGCCAACGTGA
Translation:
MLERIIRASIAHRWLVLLLVLALSGLGIWNYSRLPIDAIPDITNVQVQINTEAPGYSPMEAEQRVTFPVETA
LAGLARLDYTRSISRYGLSQVTAVFEDGTDIYFARQQVAERLQQASSQLPTGLNPSLGPVATGLGEIFMYTL
EPEKGYEEVWTPTALRTLQDWSVRPQLRNLKGVTEVNTIGGYVRQFHITPDPVKLLAYGLTMNDVLNSVARN
NANVGAGYIERHGEQYLIRIPGQVGDIERLRKIVVATRDGLPLRMGDVAEVLEGSELRTGAATKDGKEVVLG
TAFMLIGENSRAVAQRTVEKLAEIDATLPEGVRAHAVYDRTQLVDRTIATVQKNLLEGALLVIAVLFLLLGN
MRAALITATVIPLAMLLTITGMVQNRISANLMSLGALDFGLIVDGAVIIVENCLRRFSERQHQLGRLLTRDE
RFSLVSSASAEVIKPALFGLFIITAVYLPIFALSGVEGKMFHPMALTVVIALTGAMVLSLTFVPAAVALFVT
GKVSEKETKVMRGISRFYAPLLKQAIKLRVAIVAAAAVLVVLSGLLAARLGTEFIPNLDEGDIALHAMRIPG
TSLTQAIGMQRQLEARIKEFPEVEEVVAKIGTAEVATDPMPPSVADTFIMLKDRSEWPDPRKPRAVLLAELE
KAVSAIPGNNYEFTQPVQMRMNELIAGVRAEVAIKVYGDDMEQLAEIGSKIEAVAESIDGSADVALEQITGL
PLMTITPNVDALARYGLAIDDIQQTVAIALGGAVAGQVFEGDRRFDIVVRLPEAQRQDPKALATLPIPIQAG
TVAGLGQAAYVPLGQLASIEVAPGPNQISRENGKRRIVITSNVRGRDLGSFVEELRERVSREITLPEGYWVS
YGGTFEQLISASQRLSVVVPAVLVLIFGLLFMAFGSGRDAMVVFSGVPLALTGGVAALWLRDIPLSISAGVG
FIALSGVAVLNGLVMVSFIRKLHHDGAPIQDAIVNGAMTRLRPVLMTALVASLGFVPMAINVGTGAEVQRPL
ATVVIGGIVSSTLLTLLVLPALYRLVYRHKNTERVPT*
AnnotationID:412GeneID:403
AnnotatorUID: danlee
Modification Date/Time: 2005-09-30 15:03:36
Gene Name:
Confidence Code:3
GeneProduct:putative cation efflux system protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(19) Protein secretion/export apparatus
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00873, COG0841
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gi|13423925|gb|AAK24361.1|  AcrB/AcrD/AcrF family protein [Caulobacter crescentus CB15]
     Length=1057
     Score = 1424 bits (3686),  Expect = 0.0
     Identities = 721/1046 (68%), Positives = 859/1046 (82%), Gaps = 7/1046 (0%)

gi|47572228|ref|ZP_00242273.1|  COG3696: Putative silver efflux pump [Rubrivivax gelatinosus PM1]
     Length=1061
     Score = 1422 bits (3681),  Expect = 0.0
     Identities = 721/1054 (68%), Positives = 869/1054 (82%), Gaps = 15/1054 (1%)

gi|67088286|gb|EAM07752.1|  Heavy metal efflux pump CzcA [Azotobacter vinelandii AvOP]
     Length=1074
     Score = 1399 bits (3622),  Expect = 0.0
     Identities = 716/1054 (67%), Positives = 859/1054 (81%), Gaps = 17/1054 (1%)

gi|21244877|ref|NP_644459.1|  cation efflux system protein [Xanthomonas axonopodis pv. citri str. 306]
     Length=1064
     Score = 1385 bits (3584),  Expect = 0.0
     Identities = 711/1058 (67%), Positives = 850/1058 (80%), Gaps = 16/1058 (1%)








 
Structural Features:
pfam00873, ACR_tran, AcrB/AcrD/AcrF family. Members of this family are integral membrane proteins.
Some are involved in drug resistance. AcrB cooperates with a membrane fusion protein, 
AcrA, and an outer membrane channel TolC. The structure shows the AcrB forms a homotrimer. .
             CD-Length = 1017 residues,  98.4% aligned
             Score =  873 bits (2258), Expect = 0.0

COG0841, AcrB, Cation/multidrug efflux pump [Defense mechanisms].
             CD-Length = 1009 residues,  95.5% aligned
             Score =  515 bits (1328), Expect = 1e-146






   
Genomic Context:






   
Comment:


















    ReferenceID:809




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17951




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:403
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
31253
                           gb|AAK24361.1|
                           (AE005908) AcrB/AcrD/AcrF family protein [Caulobacter crescentus CB15]
                           1063
                           67.82
                           80.80
                           24
                           3692.0
     

     
     
31255
                           gb|AAM43257.1|
                           (AE012525) cation efflux system protein [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           1079
                           66.82
                           78.49
                           38
                           3594.0
     

     
     
31254
                           gb|AAM38995.1|
                           (AE012066) cation efflux system protein [Xanthomonas axonopodis pv. citri str. 306]
                           1068
                           66.76
                           79.68
                           27
                           3594.0
     

     
     
687
                           PA2520_tr
                           translation of PA2520
                           1054
                           61.29
                           77.70
                           12
                           3292.0
     

     
     
31261
                           gb|AAG05908.1|AE004679_2
                           (AE004679) RND divalent metal cation efflux transporter CzcA [Pseudomonas aeruginosa PAO1]
                           1054
                           61.29
                           77.70
                           12
                           3292.0
     

     






      

          
GC ORFID: 43405How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2520Glimmer Score: 

          
Start: 2256885Stop: 2260022

          
Length: 3138


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2520 translation of PA2520Homolog Bit Score 1269.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6877 (ORF: BLASTX 1265.0), t_PA2520 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTCGAACGTATCATTCGCGCGTCCATCGCGCATCGCTGGCTGGTGCTTCTACTGGTGCTCGCCCTGTCC 
GGGCTGGGGATCTGGAACTACAGCCGCTTGCCGATCGACGCGATCCCGGACATCACCAATGTCCAAGTGCAG 
ATCAATACCGAGGCACCGGGCTACTCGCCAATGGAGGCAGAGCAGCGCGTCACCTTCCCGGTTGAGACGGCA 
TTGGCTGGCCTTGCTCGGCTGGACTACACACGTTCGATTTCCCGCTACGGTCTGTCGCAGGTCACGGCGGTC 
TTTGAGGACGGCACTGACATCTACTTTGCTCGCCAGCAGGTCGCCGAACGCTTGCAGCAGGCATCCTCGCAG 
CTTCCGACGGGCTTGAATCCTTCCCTCGGGCCGGTGGCAACCGGCCTCGGCGAGATATTCATGTACACCTTG 
GAACCCGAGAAGGGCTACGAGGAAGTATGGACGCCAACGGCGCTGCGCACGTTGCAGGACTGGTCAGTGCGT 
CCGCAGCTTCGCAACCTGAAAGGCGTCACCGAGGTCAACACCATCGGCGGCTACGTGCGCCAATTCCACATC 
ACGCCCGATCCGGTCAAGTTGCTGGCCTACGGGTTGACGATGAACGACGTGCTGAACTCCGTCGCGCGCAAC 
AACGCGAACGTCGGTGCCGGGTACATCGAGCGTCATGGCGAACAGTATCTGATCCGCATTCCCGGCCAAGTG 
GGCGACATAGAGCGGCTGCGGAAGATTGTCGTGGCAACGCGCGATGGCCTTCCGCTCCGAATGGGCGATGTG 
GCGGAAGTGCTGGAAGGCAGCGAATTGCGAACCGGCGCTGCAACGAAAGACGGCAAGGAGGTCGTGTTGGGT 
ACGGCCTTCATGCTGATTGGCGAGAACAGCCGAGCGGTTGCGCAACGCACGGTGGAAAAGCTCGCTGAAATC 
GACGCGACACTTCCCGAGGGAGTCCGCGCGCATGCGGTTTACGACCGCACGCAGCTCGTTGATCGCACCATC 
GCCACCGTGCAGAAGAATCTTCTGGAAGGCGCATTGCTGGTGATCGCAGTGCTTTTCCTGCTGCTGGGCAAC 
ATGCGGGCAGCTCTGATAACTGCTACCGTGATTCCGCTTGCCATGCTGCTGACGATTACCGGCATGGTGCAA 
AACCGTATCTCGGCCAATCTGATGAGCCTGGGCGCGCTGGACTTCGGCCTGATCGTCGATGGCGCGGTCATC 
ATCGTGGAGAACTGCTTGCGCAGGTTCAGCGAGCGACAGCACCAGCTCGGTCGCCTGCTGACCCGCGATGAG 
CGTTTCTCGCTGGTTTCGAGTGCCAGTGCGGAAGTCATCAAGCCCGCCTTGTTCGGGCTGTTCATCATCACG 
GCTGTTTACCTTCCGATCTTCGCGTTGTCGGGCGTGGAAGGAAAGATGTTCCATCCGATGGCCCTGACCGTC 
GTCATCGCCTTGACCGGCGCGATGGTGTTGTCGCTGACCTTCGTGCCCGCTGCGGTCGCCCTGTTTGTCACC 
GGCAAGGTTTCCGAGAAGGAAACCAAGGTGATGCGCGGCATCAGCCGCTTCTATGCACCGCTTCTCAAGCAG 
GCGATTAAGTTGAGAGTCGCCATCGTCGCGGCAGCGGCAGTGCTGGTGGTGCTGTCCGGCCTGCTTGCAGCA 
CGCTTGGGAACCGAGTTCATCCCGAACCTGGATGAAGGCGACATCGCCCTGCACGCGATGCGTATCCCCGGA 
ACGAGTCTGACGCAGGCGATTGGCATGCAACGCCAACTGGAAGCCCGGATCAAGGAGTTTCCCGAGGTGGAG 
GAAGTCGTCGCCAAGATCGGCACCGCAGAGGTTGCGACCGACCCCATGCCGCCTTCGGTCGCTGACACCTTC 
ATCATGCTGAAGGATCGCAGCGAATGGCCCGATCCACGCAAGCCGAGGGCCGTACTGCTCGCCGAACTGGAG 
AAGGCGGTGAGCGCCATTCCCGGCAACAACTACGAGTTCACTCAACCCGTGCAGATGCGCATGAATGAGTTG 
ATTGCAGGTGTTCGTGCCGAGGTGGCAATCAAGGTGTACGGCGACGACATGGAGCAACTGGCAGAAATCGGC 
TCGAAGATCGAAGCCGTGGCCGAGAGTATCGACGGGTCTGCCGATGTGGCGCTTGAGCAGATTACGGGCCTG 
CCGCTGATGACGATCACGCCGAATGTGGATGCACTGGCTCGGTACGGCCTGGCGATCGACGACATACAGCAG 
ACGGTGGCGATCGCGCTAGGCGGTGCCGTCGCTGGCCAGGTGTTTGAGGGAGATCGCCGATTCGACATTGTG 
GTTCGGCTACCCGAAGCGCAACGCCAAGACCCGAAGGCACTGGCGACACTGCCCATTCCGATTCAAGCCGGA 
ACGGTTGCCGGGCTAGGCCAAGCAGCCTATGTGCCACTCGGCCAGCTTGCGTCCATCGAAGTAGCGCCCGGC 
CCCAATCAGATCAGCCGCGAGAACGGCAAACGCCGGATCGTTATCACCAGCAACGTGCGCGGGCGCGATCTA 
GGTTCGTTCGTGGAAGAACTACGTGAGCGGGTGAGCCGTGAAATCACACTGCCCGAAGGTTATTGGGTCAGC 
TACGGCGGCACGTTCGAGCAACTTATCTCGGCCAGCCAACGCCTGTCCGTCGTCGTGCCGGCCGTGCTGGTT 
CTGATTTTCGGCCTGCTGTTCATGGCCTTCGGCTCGGGCCGGGATGCAATGGTCGTCTTTAGCGGCGTTCCG 
CTGGCGCTAACGGGTGGTGTAGCCGCATTGTGGCTACGCGATATTCCGCTCTCGATTTCAGCAGGTGTCGGC 
TTCATTGCGCTGTCCGGCGTGGCTGTGCTCAACGGACTGGTGATGGTGAGCTTCATTCGCAAGCTGCATCAC 
GACGGCGCACCGATTCAGGACGCCATCGTGAATGGCGCGATGACGCGACTGCGCCCAGTGCTGATGACCGCG 
CTGGTTGCAAGCCTTGGTTTCGTACCGATGGCCATCAATGTCGGAACAGGCGCGGAAGTGCAAAGACCACTC 
GCCACTGTCGTCATCGGCGGCATCGTTTCCTCGACGCTGCTGACGCTGCTGGTTCTACCTGCGCTGTACCGG 
CTGGTCTATCGCCACAAAAACACCGAGAGGGTGCCAACGTGA