ViewGene

Locus Name:
GeneID:
Location:

GeneID:4029
TrivialName: PA14_20150
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20150
PAO1 Ortholog Locus:PA3396
Sequence Length:537
Protein Length:178
Start: 1737325
Stop: 1736789
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06784.1|AE004760_12 (AE004760) NosL protein [Pseudomonas aeruginosa PAO1]
Identities = 177/178 (99%), Positives = 178/178 (100%)
Sequence:
ATGCAACGTCATACCCTCCCCCTGCGCCCTCTGCTCGGCACGCTCCTGCTCGGCCTGCTGCTGGCCGGCTGC
GATGCGTCGCGGGACGACGCCACGGCACTCGGCCCGGTGCCGATCGCCAGCGGCGACGAATGCCACGTCTGC
GGCATGCTCATCGAGGAAATGCCCGGTCCCAAGGGCGAGGCGGTGCTTCCCGGCGCGGTGCGCAAGTTCTGC
TCCACCGCCGAACTGTTCGGCTGGTGGCTGCAACCGGAAAACCGCCAGGGCCAGGCGCGCCTGTATGTCCAC
GACATGAGCCAGGCCGACTGGCGGCACCCCGACGACGCCCGCCTGATCGACGCCACCCGCGCCTACTACGTG
GTCGGCATCCAGCGGCCGGGCGGCATGGGCGCCACCCTCGCCTCGTTCGCCGACGAAGAGGCCGCGACGCGC
CTGGCGGCCGAGGAAGGCGGCCGGGTACTGCGCTTCGACGAGATCGACCAGGCGGTGCTGCAAGGCGTGGGC
GGCGACCACCCGCACCGGCACCCGGCGCACTGA
Translation:
MQRHTLPLRPLLGTLLLGLLLAGCDASRDDATALGPVPIASGDECHVCGMLIEEMPGPKGEAVLPGAVRKFC
STAELFGWWLQPENRQGQARLYVHDMSQADWRHPDDARLIDATRAYYVVGIQRPGGMGATLASFADEEAATR
LAAEEGGRVLRFDEIDQAVLQGVGGDHPHRHPAH*
AnnotationID:4036GeneID:4029
AnnotatorUID: diggins
Modification Date/Time: 2005-04-18 09:09:41
Gene Name:nosL
Confidence Code:2
GeneProduct:putative nitrous oxide reductase protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam05573,COG4314
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:
Homology:
>dbj|BAC00878.1| (AB054991) NosL [Pseudomonas sp. MT-1]
          Length = 183

 Score =  199 bits (505), Expect = 8e-51
 Identities = 99/168 (58%), Positives = 114/168 (67%), Gaps = 1/168 (0%)
Structural Features:
pfam05573, NosL, NosL.
CD-Length = 152 residues, 100.0% aligned
Score =  196 bits (499), Expect = 1e-51
Identities = 80/153 (52%), Positives = 94/153 (61%), Gaps = 5/153 (3%)

COG4314, NosL, Predicted lipoprotein involved in nitrous oxide reduction 
[Energy production and conversion]
CD-Length = 176 residues, 100.0% aligned
Score =  229 bits (585), Expect = 2e-61
Identities = 88/177 (49%), Positives = 104/177 (58%), Gaps = 6/177 (3%)
Genomic Context:
Comment:
ReferenceID:8060
Author/Investigator(s): GenNotator
Title:
PubMed:
MedLine:
Source:
Reference Type:BLASTP
Data:
URL:
ReferenceID:25203
Author/Investigator(s): GenNotator
Title: CDD Search
PubMed:
MedLine:
Source:
Reference Type:RPSBLAST
Data:
URL:
ReferenceID:32167
Author/Investigator(s):
Title: CDD Search
PubMed:
MedLine:
Source:
Reference Type:Misc (text/plain)
Data:4029_cdd.html
URL:
ReferenceID:34062
Author/Investigator(s): Velasco L, Mesa S, Xu CA, Delgado MJ, Bedmar EJ.
Title: Molecular characterization of nosRZDFYLX genes coding for denitrifying nitrous oxide reductase of Bradyrhizobium japonicum.
PubMed: 15028871
MedLine:
Source:
Antonie Van Leeuwenhoek. 2004 Apr;85(3):229-35.
Reference Type:Journal
Data:
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15028871
ReferenceID:34063
Author/Investigator(s): Honisch U, Zumft WG.
Title: Operon structure and regulation of the nos gene region of Pseudomonas stutzeri, encoding an ABC-Type ATPase for maturation of nitrous oxide reductase.
PubMed: 12618453
MedLine:
Source:
J Bacteriol. 2003 Mar;185(6):1895-902.
Reference Type:Journal
Data:
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12618453
ReferenceID:34064
Author/Investigator(s): McGuirl MA, Bollinger JA, Cosper N, Scott RA, Dooley DM.
Title: Expression, purification, and characterization of NosL, a novel Cu(I) protein of the nitrous oxide reductase (nos) gene cluster.
PubMed: 11293413
MedLine:
Source:
J Biol Inorg Chem. 2001 Feb;6(2):189-95.
Reference Type:Journal
Data:
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11293413
ReferenceID:34065
Author/Investigator(s): Inatomi K.
Title: Analysis of the nitrous oxide reduction genes, nosZDFYL, of Achromobacter cycloclastes.
PubMed: 10048486
MedLine:
Source:
DNA Res. 1998 Dec 31;5(6):365-71.
Reference Type:Journal
Data:
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10048486
Homologs By Global Alignment
Gene ID:4029

Identity:

0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100%
HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score
6269 PA3396_tr translation of PA3396 178 99.43 99.99 0 950.0
172905 gb|AAG06784.1|AE004760_12 (AE004760) NosL protein [Pseudomonas aeruginosa PAO1] 178 99.43 99.99 0 950.0
172906 dbj|BAC00878.1| (AB054991) NosL [Pseudomonas sp. MT-1] 188 53.19 62.76 15 502.0
172907 emb|CAA93438.1| (Z69589) NosL protein [Pseudomonas stutzeri] 197 51.77 59.89 26 488.0
172908 gb|AAN53566.1|AE015496_10 (AE015496) nosL protein [Shewanella oneidensis MR-1] 180 37.77 50.55 19 306.0
GC ORFID: 47031How Found: BLASTX
GC_TrimmedSeqID: 69Blast Result ID
Subject Sequence Name: t_PA3396Glimmer Score:
Start: 3216607Stop: 3217143
Length: 537
Start Codon: ATGTruncated Start:
Stop Codon: TGATruncated Stop:
Homolog: t_PA3396 translation of PA3396Homolog Bit Score 370.0
Other Homologs: t_PA3396 (ORF: GLIMMER)
GC ORF Sequence
ATGCAACGTCATACCCTCCCCCTGCGCCCTCTGCTCGGCACGCTCCTGCTCGGCCTGCTGCTGGCCGGCTGC
GATGCGTCGCGGGACGACGCCACGGCACTCGGCCCGGTGCCGATCGCCAGCGGCGACGAATGCCACGTCTGC
GGCATGCTCATCGAGGAAATGCCCGGTCCCAAGGGCGAGGCGGTGCTTCCCGGCGCGGTGCGCAAGTTCTGC
TCCACCGCCGAACTGTTCGGCTGGTGGCTGCAACCGGAAAACCGCCAGGGCCAGGCGCGCCTGTATGTCCAC
GACATGAGCCAGGCCGACTGGCGGCACCCCGACGACGCCCGCCTGATCGACGCCACCCGCGCCTACTACGTG
GTCGGCATCCAGCGGCCGGGCGGCATGGGCGCCACCCTCGCCTCGTTCGCCGACGAAGAGGCCGCGACGCGC
CTGGCGGCCGAGGAAGGCGGCCGGGTACTGCGCTTCGACGAGATCGACCAGGCGGTGCTGCAAGGCGTGGGC
GGCGACCACCCGCACCGGCACCCGGCGCACTGA