ViewGene
Locus Name:
GeneID:
Location:

GeneID:4023
TrivialName: PA14_22710
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_22710
PAO1 Ortholog Locus:PA3208
Sequence Length:561
Protein Length:186
Start: 1975607
Stop: 1976167
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06596.1|AE004744_11 (AE004744) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 184/186 (98%), Positives = 185/186 (99%)
Sequence:
ATGGAGGCTCTCGACGCCTTGCTCACTCGCGTTTCCCACGCCCGCCTGAGCGATCCCGCGCCCTCGCCGGAG
CAGCTCGACCGGCTGTTCCGGGTCGCCTTGCGTGCCCCGGACCACGGGCAACTGCGGCCCTGGCGCTTCATC
GTGGTAGAAGGCGAAGGCCGCCGGGCCCTGGGCGACCTCTACGCCCGCGCCCTGGCGAGCCGCCAGCCGGAC
GCCGCCGAGGAAGCGCTGGCCAAGGCCCGCAACATGCCGCTGCGGGCACCGCTGCTGGTCGTGGCGGTCGCC
TGCCTGCAGGATCACCCGAAGGTGCCGCATGGCGAGCAACTGCTGGCCGCCGGCTGCGCTGCCCACGGCCTG
GTGCTGGGCGCCTATGCCCAGGGACTGGGGGCGATGTGGCGGACCGGCGAGATGGCCACCGATCCGGTGGTG
CACGCCGGCCTGGGGCTCGGCGAGCAGGAGCGGATCATCGGCTTCGTCTACCTGGGTACGCCGATGGGCGAG
TTGCGCACGCCGGCGGAACTCGACCCGACGGCCTTCGTCAGCGCCTGGCCGGGCTGA
Translation:
MEALDALLTRVSHARLSDPAPSPEQLDRLFRVALRAPDHGQLRPWRFIVVEGEGRRALGDLYARALASRQPD
AAEEALAKARNMPLRAPLLVVAVACLQDHPKVPHGEQLLAAGCAAHGLVLGAYAQGLGAMWRTGEMATDPVV
HAGLGLGEQERIIGFVYLGTPMGELRTPAELDPTAFVSAWPG*
AnnotationID:4030GeneID:4023
AnnotatorUID: diggins
Modification Date/Time: 2005-04-14 08:08:51
Gene Name:
Confidence Code:3
GeneProduct:putative nitroreductase family protein
Cell Localization:()
Synonyms:ydjA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00881,COG0778
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70080.1|AE016647_10 (AE016790) nitroreductase family protein [Pseudomonas putida
           KT2440]
          Length = 185

 Score =  233 bits (593), Expect = 6e-61
 Identities = 115/184 (62%), Positives = 143/184 (77%), Gaps = 1/184 (0%)

>gb|AAO55322.1| (AE016862) nitroreductase family protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 186

 Score =  200 bits (509), Expect = 3e-51
 Identities = 100/186 (53%), Positives = 126/186 (67%)

>gb|AAN80627.1|AE016761_202 (AE016761) Protein ydjA [Escherichia coli CFT073]
          Length = 189

 Score =  160 bits (406), Expect = 3e-39
 Identities = 79/183 (43%), Positives = 115/183 (62%), Gaps = 3/183 (1%)






 
Structural Features:
pfam00881, Nitroreductase, Nitroreductase family. 
Members of this family utilise FMN as a cofactor.
CD-Length = 176 residues, 100.0% aligned
Score = 83.2 bits (205), Expect = 2e-17
Identities = 42/176 (23%), Positives = 66/176 (37%), Gaps = 18/176 (10%)

COG0778, NfnB, Nitroreductase [Energy production and conversion]
CD-Length = 207 residues,  89.4% aligned
Score = 78.8 bits (193), Expect = 4e-16
Identities = 63/185 (34%), Positives = 88/185 (47%), Gaps = 17/185 (9%)






   
Genomic Context:






   
Comment:


















    ReferenceID:8048




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25191




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32337




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:4023_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:4023
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6261
                           PA3208_tr
                           translation of PA3208
                           186
                           98.92
                           99.46
                           0
                           956.0
     

     
     
172797
                           gb|AAG06596.1|AE004744_11
                           (AE004744) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           186
                           98.92
                           99.46
                           0
                           956.0
     

     
     
172798
                           gb|AAN70080.1|AE016647_10
                           (AE016790) nitroreductase family protein [Pseudomonas putida KT2440]
                           186
                           61.82
                           76.88
                           1
                           591.0
     

     
     
172799
                           gb|AAO55322.1|
                           (AE016862) nitroreductase family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           186
                           53.76
                           67.74
                           0
                           509.0
     

     
     
172817
                           emb|CAE26835.1|
                           (BX572597) nitroreductase family proteins [Rhodopseudomonas palustris CGA009]
                           188
                           44.14
                           61.17
                           8
                           364.0
     

     






      

          
GC ORFID: 47025How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3208Glimmer Score: 

          
Start: 2978324Stop: 2977764

          
Length: 561


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3208 translation of PA3208Homolog Bit Score 372.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7777 (ORF: BLASTX 146.0), t_PA3208 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGAGGCTCTCGACGCCTTGCTCACTCGCGTTTCCCACGCCCGCCTGAGCGATCCCGCGCCCTCGCCGGAG 
CAGCTCGACCGGCTGTTCCGGGTCGCCTTGCGTGCCCCGGACCACGGGCAACTGCGGCCCTGGCGCTTCATC 
GTGGTAGAAGGCGAAGGCCGCCGGGCCCTGGGCGACCTCTACGCCCGCGCCCTGGCGAGCCGCCAGCCGGAC 
GCCGCCGAGGAAGCGCTGGCCAAGGCCCGCAACATGCCGCTGCGGGCACCGCTGCTGGTCGTGGCGGTCGCC 
TGCCTGCAGGATCACCCGAAGGTGCCGCATGGCGAGCAACTGCTGGCCGCCGGCTGCGCTGCCCACGGCCTG 
GTGCTGGGCGCCTATGCCCAGGGACTGGGGGCGATGTGGCGGACCGGCGAGATGGCCACCGATCCGGTGGTG 
CACGCCGGCCTGGGGCTCGGCGAGCAGGAGCGGATCATCGGCTTCGTCTACCTGGGTACGCCGATGGGCGAG 
TTGCGCACGCCGGCGGAACTCGACCCGACGGCCTTCGTCAGCGCCTGGCCGGGCTGA