ViewGene
Locus Name:
GeneID:
Location:

GeneID:397
TrivialName: PA14_47090
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_47090
PAO1 Ortholog Locus:PA1327
Sequence Length:1929
Protein Length:642
Start: 4195932
Stop: 4194004
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04716.1|AE004562_3 (AE004562) probable protease [Pseudomonas aeruginosa PAO1]
Identities = 636/641 (99%), Positives = 638/641 (99%)
Sequence:
ATGCGCAAGCGCAACCCCCGGCGGGCCACGCCGGAAGAACTGCGGGTCTGCTCGGTACTGAAGGAAAAGGGC
ACCCGCGAGGAACTCGCCCACCTCGCCGCCCGCGGTGAGTGGAGCAACGTGCCGGCATACAACGCCAGCCGC
GAGCAGATCGCCGACCACCTGGGCAAGAGCGTGTCGCCGGAATCCGACGCCTCGTTCTTCGACTCGCGGCGT
TTCAGCGAGGCGATCATCTTGCTCAAGAGCCGGCCGGTGCTGCTGGTGAAGAACGGTATCTTCGACGAAGCG
CCGCTGGCCGGCCTGGAGCAGCGCCTGAAGAAGCATCGCGCCGCGCTGGCCACGCCGATCCGCTCGGTCGGT
CGGCTGGAATTGATCGACCACGACAGCATGGACTGGTGCGGTACCGGCTGGCGCATCGAGGACGACCTGATC
GTCACCAACCGGCATGTCGCCAGCCTGTTCGCCGAACGCCAGGGCAGCCTGTTCCGCTTCCGTCTCAACCAG
GCTGGCAAGCAGGTGCGCACCCGCGTGGACTTCCGCGAGGAATACCGCCAGCCGGAAAGCGACGAGCATGTG
ATCGCGCGGGTCCTGTGGATCGCCCCGGACGTCTCCGAGGCGCCGGACATGGCGATCCTCCAGGTAGTGAAG
GGCGACCTGCTGCCGCCGCCGCTGGTCCTCGCGCGCCAGGATGCCGAGCCGCGCCAGGCGGTGGCGGTGGTC
GGCTATCCGGCGCGCGACAGCCGCAACGACGCCGGGGCGATGGAAGATATCTTCGGCAACATCTACGACGTC
AAGCGTTTCGCTCCCGGCGAAGTCGTGGGATTGCCCCATGACGCCTGGTACCTCACCCACGACTGCTCGACC
CTGGGCGGCAATTCTGGCTCGGCGGTGCTTTCGATCGACGGCGGCGAGGTGGTCGGCCTGCACTTCGGCGGC
CAGTTCCGCAAGACCAACTATGCGGTGAAGGCCAGCGTGATCCGCAGCCTGCTGGCGCGTCGCGCCTGGGTG
CCGGTGGCCGGGGCGGAGCTGAAGCGCGGCGCGCCGCGCTTCCAGGAAAAGCAGCGCAGCGTCGCCGACCTG
GCCGAGCGAAAGGGTTTCGACGAGGCCTTCCTCGGGCCGAAGGCGGCCTTGCCCAAGCCGGGCAAGAGCCAC
CAGGTGCTGCCGGTGGGCAAGGGCACGCGCCTGGACTATCTGCATTTCTCGGTGGTGATGAGCGCCAGCCGG
CGCTTGCCGATCCTCACCGCGGTGAATATCGACGGCGCGCTCAAGCGTTCGCTCAAGCGCAAGGATTCCTGG
GGCTTCGACCCGCGCATCGAGGCGGCCGTGCAGGTCGGACACAAGGACTTCTACGGTCCGGCTTCGTTCGAC
AAGGGCCACATGGTGCGTCGCGAGGATCCCGGCTGGGGCGACAGCGATGCCATCGCCCGGCAGGCCGAGGAC
GACACCTTCGTCTACACCAACGCGGTACCCCAGGTGGCGCAGCTCAACCAGCGCAGCTGGCTGTCGCTGGAG
GACTACGTGCTGCAGAACGCCAGGAGCGTGGGCTTCCGCATCTCGGTGTTCACCGGCCCGGTGTTCCGCGAC
GACGATCCGCTCTACCAGGGCGTGCAGGTGCCGCTGGAGTTCTGGAAGGTGGTGGCGATGATCGACGCCGAC
AGCGGCGAACTGGGAGTGTCCGCCTACCTGCTGGGGCAGGAAGGGATGATGCCCAGCGAAGGCTTCCGCTAC
GGTGCGTTCAAGACCTACCAGGTGCCGTTGGCGAAGGTCGAGGCCAGCGCCGACCTGCGTTTCTCCAGCGCC
CTGCGCAAGGCCGACGTGCTCGCCGGCACGCCGCTGGAGGAGGCGCTGGAAAGCGGACGCTTCATCGAGATC
GACGGACCGGACGACCTCCTGCTGAGCCGGCCGGGGCCGGCCGGCAAGGGCCGCTGA
Translation:
MRKRNPRRATPEELRVCSVLKEKGTREELAHLAARGEWSNVPAYNASREQIADHLGKSVSPESDASFFDSRR
FSEAIILLKSRPVLLVKNGIFDEAPLAGLEQRLKKHRAALATPIRSVGRLELIDHDSMDWCGTGWRIEDDLI
VTNRHVASLFAERQGSLFRFRLNQAGKQVRTRVDFREEYRQPESDEHVIARVLWIAPDVSEAPDMAILQVVK
GDLLPPPLVLARQDAEPRQAVAVVGYPARDSRNDAGAMEDIFGNIYDVKRFAPGEVVGLPHDAWYLTHDCST
LGGNSGSAVLSIDGGEVVGLHFGGQFRKTNYAVKASVIRSLLARRAWVPVAGAELKRGAPRFQEKQRSVADL
AERKGFDEAFLGPKAALPKPGKSHQVLPVGKGTRLDYLHFSVVMSASRRLPILTAVNIDGALKRSLKRKDSW
GFDPRIEAAVQVGHKDFYGPASFDKGHMVRREDPGWGDSDAIARQAEDDTFVYTNAVPQVAQLNQRSWLSLE
DYVLQNARSVGFRISVFTGPVFRDDDPLYQGVQVPLEFWKVVAMIDADSGELGVSAYLLGQEGMMPSEGFRY
GAFKTYQVPLAKVEASADLRFSSALRKADVLAGTPLEEALESGRFIEIDGPDDLLLSRPGPAGKGR*
AnnotationID:406GeneID:397
AnnotatorUID: danlee
Modification Date/Time: 2006-02-07 12:12:12
Gene Name:
Confidence Code:3
GeneProduct:putative protease
Cell Localization:()
Synonyms:PA1327
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:putative DNA/RNA nuclease
Functional Category Confidence Code:5
COGs:COG1864,cd00091
Secondary Functional Category(ies):nucleotide biosynthesis and metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAK22531.1| (AE005728) nuclease, putative [Caulobacter crescentus CB15]
          Length = 695

 Score =  327 bits (839), Expect = 1e-88
 Identities = 240/612 (39%), Positives = 326/612 (53%), Gaps = 70/612 (11%)

>gb|AAL95476.1| (AE010634) Serine protease, V8 family; Endonuclease [Fusobacterium
           nucleatum subsp. nucleatum ATCC 25586]
          Length = 579

 Score =  199 bits (507), Expect = 3e-50
 Identities = 135/347 (38%), Positives = 189/347 (54%), Gaps = 29/347 (8%)

>gb|AAO55981.1| (AE016864) DNA/RNA non-specific endonuclease [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 316

 Score =  196 bits (498), Expect = 4e-49
 Identities = 118/280 (42%), Positives = 159/280 (56%), Gaps = 17/280 (6%)








 
Structural Features:
COG1864, NUC1, DNA/RNA endonuclease G, NUC1 
[Nucleotide transport and metabolism]
CD-Length = 281 residues,  98.2% aligned
Score =  256 bits (656), Expect = 4e-69
Identities = 111/302 (36%), Positives = 143/302 (47%), Gaps = 29/302 (9%)

cd00091, NUC, DNA/RNA non-specific endonuclease
CD-Length = 241 residues,  93.4% aligned
Score =  196 bits (499), Expect = 8e-51
Identities = 73/232 (31%), Positives = 103/232 (44%), Gaps = 21/232 (9%)






   
Genomic Context:






   
Comment:
Confirmed frame-shift results in premature stop and truncation of last 15 amino acids of PAO1 PA1327.


















    ReferenceID:797




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17939




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37467




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:397_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:397
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
663
                           PA1327_tr
                           translation of PA1327
                           656
                           96.95
                           97.25
                           14
                           3292.0
     

     
     
30487
                           gb|AAG04716.1|AE004562_3
                           (AE004562) probable protease [Pseudomonas aeruginosa PAO1]
                           656
                           96.95
                           97.25
                           14
                           3292.0
     

     
     
30488
                           gb|AAO55981.1|
                           (AE016864) DNA/RNA non-specific endonuclease [Pseudomonas syringae pv. tomato str. DC3000]
                           656
                           19.81
                           27.13
                           354
                           509.0
     

     






      

          
GC ORFID: 43399How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1327Glimmer Score: 

          
Start: 757977Stop: 759905

          
Length: 1929


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1327 translation of PA1327Homolog Bit Score 1273.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene252 (ORF: BLASTX 135.0), t_PA1327 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCAAGCGCAACCCCCGGCGGGCCACGCCGGAAGAACTGCGGGTCTGCTCGGTACTGAAGGAAAAGGGC 
ACCCGCGAGGAACTCGCCCACCTCGCCGCCCGCGGTGAGTGGAGCAACGTGCCGGCATACAACGCCAGCCGC 
GAGCAGATCGCCGACCACCTGGGCAAGAGCGTGTCGCCGGAATCCGACGCCTCGTTCTTCGACTCGCGGCGT 
TTCAGCGAGGCGATCATCTTGCTCAAGAGCCGGCCGGTGCTGCTGGTGAAGAACGGTATCTTCGACGAAGCG 
CCGCTGGCCGGCCTGGAGCAGCGCCTGAAGAAGCATCGCGCCGCGCTGGCCACGCCGATCCGCTCGGTCGGT 
CGGCTGGAATTGATCGACCACGACAGCATGGACTGGTGCGGTACCGGCTGGCGCATCGAGGACGACCTGATC 
GTCACCAACCGGCATGTCGCCAGCCTGTTCGCCGAACGCCAGGGCAGCCTGTTCCGCTTCCGTCTCAACCAG 
GCTGGCAAGCAGGTGCGCACCCGCGTGGACTTCCGCGAGGAATACCGCCAGCCGGAAAGCGACGAGCATGTG 
ATCGCGCGGGTCCTGTGGATCGCCCCGGACGTCTCCGAGGCGCCGGACATGGCGATCCTCCAGGTAGTGAAG 
GGCGACCTGCTGCCGCCGCCGCTGGTCCTCGCGCGCCAGGATGCCGAGCCGCGCCAGGCGGTGGCGGTGGTC 
GGCTATCCGGCGCGCGACAGCCGCAACGACGCCGGGGCGATGGAAGATATCTTCGGCAACATCTACGACGTC 
AAGCGTTTCGCTCCCGGCGAAGTCGTGGGATTGCCCCATGACGCCTGGTACCTCACCCACGACTGCTCGACC 
CTGGGCGGCAATTCTGGCTCGGCGGTGCTTTCGATCGACGGCGGCGAGGTGGTCGGCCTGCACTTCGGCGGC 
CAGTTCCGCAAGACCAACTATGCGGTGAAGGCCAGCGTGATCCGCAGCCTGCTGGCGCGTCGCGCCTGGGTG 
CCGGTGGCCGGGGCGGAGCTGAAGCGCGGCGCGCCGCGCTTCCAGGAAAAGCAGCGCAGCGTCGCCGACCTG 
GCCGAGCGAAAGGGTTTCGACGAGGCCTTCCTCGGGCCGAAGGCGGCCTTGCCCAAGCCGGGCAAGAGCCAC 
CAGGTGCTGCCGGTGGGCAAGGGCACGCGCCTGGACTATCTGCATTTCTCGGTGGTGATGAGCGCCAGCCGG 
CGCTTGCCGATCCTCACCGCGGTGAATATCGACGGCGCGCTCAAGCGTTCGCTCAAGCGCAAGGATTCCTGG 
GGCTTCGACCCGCGCATCGAGGCGGCCGTGCAGGTCGGACACAAGGACTTCTACGGTCCGGCTTCGTTCGAC 
AAGGGCCACATGGTGCGTCGCGAGGATCCCGGCTGGGGCGACAGCGATGCCATCGCCCGGCAGGCCGAGGAC 
GACACCTTCGTCTACACCAACGCGGTACCCCAGGTGGCGCAGCTCAACCAGCGCAGCTGGCTGTCGCTGGAG 
GACTACGTGCTGCAGAACGCCAGGAGCGTGGGCTTCCGCATCTCGGTGTTCACCGGCCCGGTGTTCCGCGAC 
GACGATCCGCTCTACCAGGGCGTGCAGGTGCCGCTGGAGTTCTGGAAGGTGGTGGCGATGATCGACGCCGAC 
AGCGGCGAACTGGGAGTGTCCGCCTACCTGCTGGGGCAGGAAGGGATGATGCCCAGCGAAGGCTTCCGCTAC 
GGTGCGTTCAAGACCTACCAGGTGCCGTTGGCGAAGGTCGAGGCCAGCGCCGACCTGCGTTTCTCCAGCGCC 
CTGCGCAAGGCCGACGTGCTCGCCGGCACGCCGCTGGAGGAGGCGCTGGAAAGCGGACGCTTCATCGAGATC 
GACGGACCGGACGACCTCCTGCTGAGCCGGCCGGGGCCGGCCGGCAAGGGCCGCTGA