ViewGene
Locus Name:
GeneID:
Location:

GeneID:3961
TrivialName: PA14_66640
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66640
PAO1 Ortholog Locus:PA5042
Sequence Length:624
Protein Length:207
Start: 5950446
Stop: 5949823
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08427.1|AE004917_6 (AE004917) type 4 fimbrial biogenesis protein PilO [Pseudomonas aeruginosa PAO1]
Identities = 195/207 (94%), Positives = 202/207 (97%)
Sequence:
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGT
TCCTGGCCGGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAAC
TTCCATCTCCGTGACTTGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTT
GCTACCAAAGCGTTCCAGGCCGCGAACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAGGAGTCCTTC
GGCGCCTTGCTGCGGCAGTTGCCCAGCGACACCGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGT
CTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCCCTGAAGTCACTCAGCAGTTCTATATCGAGCTG
CCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTCGTCAGCGGCGTGTCCAGCCTGCCG
CGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTCGAAGCTGCGCATGAGC
ATCTTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA
Translation:
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQF
ATKAFQAANLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIEL
PIQISVVGGYHDLATFVSGVSSLPRIVTLHDFEIKPASPDNTSKLRMSILAKTYRYNDKGLKK*
AnnotationID:3968GeneID:3961
AnnotatorUID: diggins
Modification Date/Time: 2005-03-14 17:05:25
Gene Name:pilO
Confidence Code:1
GeneProduct:type 4 fimbrial biogenesis protein PilO
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:fimbrial assembly
Functional Category Confidence Code:5
COGs:COG3167,pfam04350
Secondary Functional Category(ies):intracellular trafficking and secretion
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAA93088.1| (L28837) membrane protein [Pseudomonas syringae]
          Length = 207

 Score =  313 bits (801), Expect = 5e-85
 Identities = 156/207 (75%), Positives = 178/207 (85%)

>gb|AAO58557.1| (AE016874) type IV pilus biogenesis protein PilO [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 207

 Score =  310 bits (794), Expect = 4e-84
 Identities = 154/207 (74%), Positives = 178/207 (85%)








 
Structural Features:
COG3167, PilO, Tfp pilus assembly protein PilO 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 211 residues,  92.9% aligned
Score =  259 bits (664), Expect = 1e-70
Identities = 110/196 (56%), Positives = 136/196 (69%)

pfam04350, PilO, Pilus assembly protein, PilO. 
CD-Length = 151 residues, 100.0% aligned
Score =  191 bits (487), Expect = 5e-50
Identities = 86/151 (56%), Positives = 103/151 (68%), Gaps = 6/151 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7924




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25067




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35036




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3961_cdd.html


    		




 
URL:

















    ReferenceID:35847




  
Author/Investigator(s):
        Martin PR, Watson AA, McCaul TF, Mattick JS.





  
Title:
        Characterization of a five-gene cluster required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa.




  
PubMed:
        7565110


MedLine:
        




    
Source:
Mol Microbiol. 1995 May;16(3):497-508.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7565110









           
Homologs By Global Alignment 

           

       



     
Gene ID:3961
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6200
                           PA5042_tr
                           translation of PA5042
                           207
                           94.20
                           97.58
                           0
                           983.0
     

     
     
171768
                           gb|AAG08427.1|AE004917_6
                           (AE004917) type 4 fimbrial biogenesis protein PilO [Pseudomonas aeruginosa PAO1]
                           207
                           94.20
                           97.58
                           0
                           983.0
     

     
     
171769
                           gb|AAA87404.1|
                           (U12892) pilO [Pseudomonas aeruginosa]
                           207
                           94.20
                           97.58
                           0
                           983.0
     

     
     
171770
                           gb|AAA93088.1|
                           (L28837) membrane protein [Pseudomonas syringae]
                           207
                           75.36
                           85.99
                           0
                           801.0
     

     
     
171771
                           gb|AAO58557.1|
                           (AE016874) type IV pilus biogenesis protein PilO [Pseudomonas syringae pv. tomato str. DC3000]
                           207
                           74.39
                           85.99
                           0
                           794.0
     

     






      

          
GC ORFID: 46963How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5042Glimmer Score: 

          
Start: 5214129Stop: 5213506

          
Length: 624


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5042 translation of PA5042Homolog Bit Score 383.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3292 (ORF: BLASTX 313.0), t_PA5042 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGT 
TCCTGGCCGGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAAC 
TTCCATCTCCGTGACTTGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTT 
GCTACCAAAGCGTTCCAGGCCGCGAACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAGGAGTCCTTC 
GGCGCCTTGCTGCGGCAGTTGCCCAGCGACACCGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGT 
CTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCCCTGAAGTCACTCAGCAGTTCTATATCGAGCTG 
CCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTCGTCAGCGGCGTGTCCAGCCTGCCG 
CGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTCGAAGCTGCGCATGAGC 
ATCTTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA