ViewGene
Locus Name:
GeneID:
Location:

GeneID:3955
TrivialName: PA14_29920
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_29920
PAO1 Ortholog Locus:PA2644
Sequence Length:549
Protein Length:182
Start: 2590463
Stop: 2589915
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06032.1|AE004693_3 (AE004693) NADH Dehydrogenase I chain I [Pseudomonas aeruginosa PAO1]
Identities = 182/182 (100%), Positives = 182/182 (100%)
Sequence:
GTGATCAAAGAAATCATCAACGTCGTGCATGGCACCTTCACCCAGCTTCGCAGCCTGGTGATGATCTTCGGC
CATGCCTTCCGCAAGCGCGATACCCTGCAATACCCCGAAGAGCCGGTCTACCTGCCGCCGCGCTATCGTGGC
CGGATCGTCCTGACCCGCGACCCCGACGGCGAGGAACGCTGCGTAGCCTGCAACCTGTGCGCCGTGGCCTGT
CCGGTTGGCTGCATCTCCCTGCAGAAGGCCGAGACCGAGGACGGTCGCTGGTATCCGGAGTTCTTCCGCATC
AACTTCTCGCGCTGCATCTTCTGCGGCCTGTGCGAGGAAGCCTGCCCGACCACCGCGATCCAGCTCACCCCG
GACTTCGAGATGGGCGAGTTCAAGCGCCAGGACCTGGTCTACGAGAAGCACGACCTGCTGATCTCCGGCCCG
GGCAAGAACCCGGACTACAACTACTACCGCGTTGCGGGGATGGCGATCGCCGGCAAGCCGAAAGGCGCCGCG
CAGAACGAAGCCGAACCGATCAACGTGAAAAGCCTGCTGCCATAA
Translation:
MIKEIINVVHGTFTQLRSLVMIFGHAFRKRDTLQYPEEPVYLPPRYRGRIVLTRDPDGEERCVACNLCAVAC
PVGCISLQKAETEDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMGEFKRQDLVYEKHDLLISGP
GKNPDYNYYRVAGMAIAGKPKGAAQNEAEPINVKSLLP*
AnnotationID:3962GeneID:3955
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 11:11:15
Gene Name:nuoI
Confidence Code:2
GeneProduct:NADH Dehydrogenase I chain I
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1143
Secondary Functional Category(ies):
EC Number:1.6.5.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL89571.1| (AF281148) NADH dehydrogenase I subunit I [Pseudomonas fluorescens]
          Length = 182

 Score =  355 bits (911), Expect = 7e-98
 Identities = 169/182 (92%), Positives = 173/182 (95%)

>gb|AAM85206.1|AE013767_3 (AE013767) NADH dehydrogenase I chain I [Yersinia pestis KIM]
          Length = 180

 Score =  315 bits (807), Expect = 9e-86
 Identities = 149/175 (85%), Positives = 160/175 (91%)

>gb|AAN81276.1|AE016763_235 (AE016763) NADH dehydrogenase I chain I [Escherichia coli CFT073]
          Length = 180

 Score =  314 bits (805), Expect = 1e-85
 Identities = 148/172 (86%), Positives = 159/172 (92%)








 
Structural Features:
COG1143, NuoI, Formate hydrogenlyase subunit 6/NADH:ubiquinone 
oxidoreductase 23 kD subunit (chain I) 
[Energy production and conversion]
CD-Length = 172 residues,  98.3% aligned
Score =  172 bits (436), Expect = 3e-44
Identities = 72/175 (41%), Positives = 95/175 (54%), Gaps = 7/175 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14972




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:25055




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33219




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3955_cdd.html


    		




 
URL:

















    ReferenceID:34579




  
Author/Investigator(s):
        Camacho Carvajal MM, Wijfjes AH, Mulders IH, Lugtenberg BJ, Bloemberg GV.





  
Title:
        Characterization of NADH dehydrogenases of Pseudomonas fluorescens WCS365 and their role in competitive root colonization.




  
PubMed:
        12118882


MedLine:
        




    
Source:
Mol Plant Microbe Interact. 2002 Jul;15(7):662-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12118882









           
Homologs By Global Alignment 

           

       



     
Gene ID:3955
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6193
                           PA2644_tr
                           translation of PA2644
                           182
                           99.99
                           99.99
                           0
                           986.0
     

     
     
171682
                           gb|AAG06032.1|AE004693_3
                           (AE004693) NADH Dehydrogenase I chain I [Pseudomonas aeruginosa PAO1]
                           182
                           99.45
                           99.99
                           0
                           983.0
     

     
     
171683
                           gb|AAL89571.1|
                           (AF281148) NADH dehydrogenase I subunit I [Pseudomonas fluorescens]
                           182
                           92.30
                           95.05
                           0
                           907.0
     

     
     
171684
                           gb|AAN69709.1|AE016607_3
                           (AE016789) NADH dehydrogenase I, I subunit [Pseudomonas putida KT2440]
                           182
                           91.20
                           94.50
                           0
                           904.0
     

     
     
171685
                           gb|AAO56850.1|
                           (AE016868) NADH dehydrogenase I, I subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           182
                           90.10
                           94.50
                           0
                           897.0
     

     






      

          
GC ORFID: 46957How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2644Glimmer Score: 

          
Start: 2363463Stop: 2364011

          
Length: 549


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2644 translation of PA2644Homolog Bit Score 384.0

          
Other Homologs:  psyr_15may02_Scaffold17_revised_gene2772 (ORF: BLASTX 350.0), t_PA2644 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGATCAAAGAAATCATCAACGTCGTGCATGGCACCTTCACCCAGCTTCGCAGCCTGGTGATGATCTTCGGC 
CATGCCTTCCGCAAGCGCGATACCCTGCAATACCCCGAAGAGCCGGTCTACCTGCCGCCGCGCTATCGTGGC 
CGGATCGTCCTGACCCGCGACCCCGACGGCGAGGAACGCTGCGTAGCCTGCAACCTGTGCGCCGTGGCCTGT 
CCGGTTGGCTGCATCTCCCTGCAGAAGGCCGAGACCGAGGACGGTCGCTGGTATCCGGAGTTCTTCCGCATC 
AACTTCTCGCGCTGCATCTTCTGCGGCCTGTGCGAGGAAGCCTGCCCGACCACCGCGATCCAGCTCACCCCG 
GACTTCGAGATGGGCGAGTTCAAGCGCCAGGACCTGGTCTACGAGAAGCACGACCTGCTGATCTCCGGCCCG 
GGCAAGAACCCGGACTACAACTACTACCGCGTTGCGGGGATGGCGATCGCCGGCAAGCCGAAAGGCGCCGCG 
CAGAACGAAGCCGAACCGATCAACGTGAAAAGCCTGCTGCCATAA