ViewGene
Locus Name:
GeneID:
Location:

GeneID:392
TrivialName: PA14_65740
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65740
PAO1 Ortholog Locus:PA4973
Sequence Length:1884
Protein Length:627
Start: 5855041
Stop: 5853158
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08358.1|AE004910_5 (AE004910) thiamin biosynthesis protein ThiC [Pseudomonas aeruginosa PAO1]
Identities = 626/627 (99%), Positives = 626/627 (99%)
Sequence:
ATGAGCGCAACGCAGAAGAACAACATCACCCGCCTTGAGCAGCTCGACCGCCAGTCGACGCAGCCTTTCCCG
AACTCGCGCAAGGTCTACCTGACCGGCTCGCGCCCGGACATCCGCGTCCCGGTGCGGGAAATCTCCCTCGCC
GACACCCCCACCGCCTTCGGCGGCGAAAAGAACCCGCCGGTGTTCGTCTACGACACCTCCGGCCCGTATACC
GACCCCGAGGTCCGCATCGATCTGCGCAAGGGACTGCCTGACGTGCGTTCGCGCTGGATCGACGAGCGCGGC
GACACCGAGATCCTCCCAGGCCTGACCTCCGAGTTCGGCCAGGCCCGCCTGGCCGACGCCAGCCTCGACGCC
CTGCGCTTCGCCCACGTGCGCACGCCGCGCCGGGCCAAGCCGGGCGCCAACGTCTCGCAGATGCACTACGCG
AAGAAGGGCATCATCACCCCGGAGATGGAATACATCGCCATCCGCGAGAACATGAAGCTGCAGGAAGCCCGG
GCCGCCGGCCTGCTCGACCAGCAGCACCCGGGGCACAGCTTCGGTGCCAACACCCCGAAGGAAATCACCCCC
GAGTTCGTCCGCGAGGAAGTCGCCCGCGGCCGCGCGATCATTCCGGCGAACATCAACCACACCGAGCTTGAG
CCGATGATCATCGGCCGCAATTTCCTGGTGAAGATCAACGGCAACATCGGCAACAGCGCGCTGGGCTCGTCG
ATCGAGGAAGAAGTGGAGAAGCTCACCTGGGGCATCCGCTGGGGCGCCGACACGGTGATGGACCTGTCCACC
GGCAAGCACATCCATGAAACCCGCGAGTGGATCCTGCGCAACAGCCCGGTACCGATCGGCACCGTACCGATC
TACCAGGCGCTGGAGAAGGTCAACGGCGTCGCCGAGGACCTGACCTGGGAAATCTTCCGCGATACCCTGATC
GAACAGGCCGAGCAGGGCGTCGACTACTTCACCATCCACGCCGGCGTGCTGCTGCGCTATGTGCCGCTGACC
GCCAAGCGGGTCACCGGCATCGTTTCCCGCGGCGGCTCGATCATGGCCAAGTGGTGCCTGGCGCACCACCAG
GAGAACTTCCTCTACACGCATTTCGAAGAGATCTGCGAAATCATGAAGGCCTACGACGTCAGCTTCTCGCTG
GGCGATGGCCTGCGTCCGGGCTCGGTGGCCGACGCCAACGACGCCGCCCAGTTCGGCGAGCTGGAAACCCTC
GGCGAGCTGACCAAGATCGCCTGGAAGCACGACGTGCAGGTGATGATCGAAGGCCCCGGCCACGTGCCGATG
CAATTGATCAAGGAAAACATGGACAAGCAGCTGGAGTGCTGCGACGAGGCGCCGTTCTACACCCTCGGCCCG
CTGACCACCGACATCGCCCCCGGCTACGACCACATCACCTCCGGCATCGGCGCGGCGATGATCGGCTGGTTC
GGCTGCGCCATGCTCTGCTACGTCACGCCCAAGGAGCACCTGGGTCTGCCGAACAAGGATGACGTGAAGACC
GGGATCATCACCTACAAGATCGCCGCCCATGCCGCCGACCTCGCCAAGGGCCACCCGGGCGCGCAGATCCGC
GACAACGCGCTGTCCAAGGCGCGCTTCGAGTTCCGTTGGGAGGACCAGTTCAACCTTGGCCTGGACCCGGAC
ACCGCGCGGGCGTTCCATGACGAGACCCTGCCGAAGGACTCGGCCAAGGTCGCGCACTTCTGCTCCATGTGC
GGACCGAAGTTCTGCTCGATGAAGATCACCCAGGAAGTCCGCGACTACGCCAAGGAGAACGGCCTGTCCGAC
GAGAGCAAGGCCATCGAGGCCGGCTTCCAGGAGCAGGCGGCGCGCTTCAAGGACGAAGGATCGGTGATCTAC
AGGCAGGTGTAG
Translation:
MSATQKNNITRLEQLDRQSTQPFPNSRKVYLTGSRPDIRVPVREISLADTPTAFGGEKNPPVFVYDTSGPYT
DPEVRIDLRKGLPDVRSRWIDERGDTEILPGLTSEFGQARLADASLDALRFAHVRTPRRAKPGANVSQMHYA
KKGIITPEMEYIAIRENMKLQEARAAGLLDQQHPGHSFGANTPKEITPEFVREEVARGRAIIPANINHTELE
PMIIGRNFLVKINGNIGNSALGSSIEEEVEKLTWGIRWGADTVMDLSTGKHIHETREWILRNSPVPIGTVPI
YQALEKVNGVAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHQ
ENFLYTHFEEICEIMKAYDVSFSLGDGLRPGSVADANDAAQFGELETLGELTKIAWKHDVQVMIEGPGHVPM
QLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKDDVKT
GIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARAFHDETLPKDSAKVAHFCSMC
GPKFCSMKITQEVRDYAKENGLSDESKAIEAGFQEQAARFKDEGSVIYRQV*
AnnotationID:401GeneID:392
AnnotatorUID: diggins
Modification Date/Time: 2005-03-14 06:06:04
Gene Name:thiC
Confidence Code:2
GeneProduct:thiamin biosynthesis protein ThiC
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam01964
Secondary Functional Category(ies):central intermediary metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70489.1|AE016690_5 (AE016792) thiamin biosynthesis protein ThiC [Pseudomonas putida
           KT2440]
          Length = 626

 Score = 1119 bits (2895), Expect = 0.0
 Identities = 543/620 (87%), Positives = 577/620 (93%), Gaps = 2/620 (0%)

>gb|AAO58404.1| (AE016874) thiamin biosynthesis protein ThiC [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 629

 Score = 1101 bits (2847), Expect = 0.0
 Identities = 537/629 (85%), Positives = 569/629 (90%), Gaps = 2/629 (0%)

>emb|CAE30802.1| (BX640437) thiamine biosynthesis protein [Bordetella
           bronchiseptica]
          Length = 637

 Score =  984 bits (2544), Expect = 0.0
 Identities = 478/625 (76%), Positives = 535/625 (85%), Gaps = 13/625 (2%)






 
Structural Features:
pfam01964, ThiC, ThiC family. ThiC is found within the thiamine biosynthesis operon. 
ThiC is involved in pyrimidine biosynthesis.
CD-Length = 424 residues, 100.0% aligned
Score =  754 bits (1947), Expect = 0.0
Identities = 307/452 (67%), Positives = 347/452 (76%), Gaps = 28/452 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:787




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17929




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35068




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:392_cdd.html


    		




 
URL:

















    ReferenceID:35882




  
Author/Investigator(s):
        Vander Horn PB, Backstrom AD, Stewart V, Begley TP.





  
Title:
        Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12.




  
PubMed:
        8432721


MedLine:
        




    
Source:
J Bacteriol. 1993 Feb;175(4):982-92.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8432721

















    ReferenceID:35883




  
Author/Investigator(s):
        Miranda-Rios J, Morera C, Taboada H, Davalos A, Encarnacion S, Mora J, Soberon M.





  
Title:
        Expression of thiamin biosynthetic genes (thiCOGE) and production of symbiotic terminal oxidase cbb3 in Rhizobium etli.




  
PubMed:
        9371431


MedLine:
        




    
Source:
J Bacteriol. 1997 Nov;179(22):6887-93.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9371431









           
Homologs By Global Alignment 

           

       



     
Gene ID:392
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
660
                           PA4973_tr
                           translation of PA4973
                           627
                           99.84
                           99.84
                           0
                           3302.0
     

     
     
30381
                           gb|AAG08358.1|AE004910_5
                           (AE004910) thiamin biosynthesis protein ThiC [Pseudomonas aeruginosa PAO1]
                           627
                           99.84
                           99.84
                           0
                           3302.0
     

     
     
30382
                           gb|AAN70489.1|AE016690_5
                           (AE016792) thiamin biosynthesis protein ThiC [Pseudomonas putida KT2440]
                           628
                           86.78
                           92.51
                           3
                           2896.0
     

     
     
30383
                           gb|AAO58404.1|
                           (AE016874) thiamin biosynthesis protein ThiC [Pseudomonas syringae pv. tomato str. DC3000]
                           629
                           85.37
                           90.46
                           2
                           2849.0
     

     
     
30384
                           gb|AAQ57914.1|
                           (AE016910) thiamin biosynthesis protein [Chromobacterium violaceum ATCC 12472]
                           637
                           75.66
                           84.77
                           15
                           2553.0
     

     






      

          
GC ORFID: 43394How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4973Glimmer Score: 

          
Start: 5118723Stop: 5116840

          
Length: 1884


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4973 translation of PA4973Homolog Bit Score 1276.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3404 (ORF: BLASTX 1100.0), t_PA4973 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGCAACGCAGAAGAACAACATCACCCGCCTTGAGCAGCTCGACCGCCAGTCGACGCAGCCTTTCCCG 
AACTCGCGCAAGGTCTACCTGACCGGCTCGCGCCCGGACATCCGCGTCCCGGTGCGGGAAATCTCCCTCGCC 
GACACCCCCACCGCCTTCGGCGGCGAAAAGAACCCGCCGGTGTTCGTCTACGACACCTCCGGCCCGTATACC 
GACCCCGAGGTCCGCATCGATCTGCGCAAGGGACTGCCTGACGTGCGTTCGCGCTGGATCGACGAGCGCGGC 
GACACCGAGATCCTCCCAGGCCTGACCTCCGAGTTCGGCCAGGCCCGCCTGGCCGACGCCAGCCTCGACGCC 
CTGCGCTTCGCCCACGTGCGCACGCCGCGCCGGGCCAAGCCGGGCGCCAACGTCTCGCAGATGCACTACGCG 
AAGAAGGGCATCATCACCCCGGAGATGGAATACATCGCCATCCGCGAGAACATGAAGCTGCAGGAAGCCCGG 
GCCGCCGGCCTGCTCGACCAGCAGCACCCGGGGCACAGCTTCGGTGCCAACACCCCGAAGGAAATCACCCCC 
GAGTTCGTCCGCGAGGAAGTCGCCCGCGGCCGCGCGATCATTCCGGCGAACATCAACCACACCGAGCTTGAG 
CCGATGATCATCGGCCGCAATTTCCTGGTGAAGATCAACGGCAACATCGGCAACAGCGCGCTGGGCTCGTCG 
ATCGAGGAAGAAGTGGAGAAGCTCACCTGGGGCATCCGCTGGGGCGCCGACACGGTGATGGACCTGTCCACC 
GGCAAGCACATCCATGAAACCCGCGAGTGGATCCTGCGCAACAGCCCGGTACCGATCGGCACCGTACCGATC 
TACCAGGCGCTGGAGAAGGTCAACGGCGTCGCCGAGGACCTGACCTGGGAAATCTTCCGCGATACCCTGATC 
GAACAGGCCGAGCAGGGCGTCGACTACTTCACCATCCACGCCGGCGTGCTGCTGCGCTATGTGCCGCTGACC 
GCCAAGCGGGTCACCGGCATCGTTTCCCGCGGCGGCTCGATCATGGCCAAGTGGTGCCTGGCGCACCACCAG 
GAGAACTTCCTCTACACGCATTTCGAAGAGATCTGCGAAATCATGAAGGCCTACGACGTCAGCTTCTCGCTG 
GGCGATGGCCTGCGTCCGGGCTCGGTGGCCGACGCCAACGACGCCGCCCAGTTCGGCGAGCTGGAAACCCTC 
GGCGAGCTGACCAAGATCGCCTGGAAGCACGACGTGCAGGTGATGATCGAAGGCCCCGGCCACGTGCCGATG 
CAATTGATCAAGGAAAACATGGACAAGCAGCTGGAGTGCTGCGACGAGGCGCCGTTCTACACCCTCGGCCCG 
CTGACCACCGACATCGCCCCCGGCTACGACCACATCACCTCCGGCATCGGCGCGGCGATGATCGGCTGGTTC 
GGCTGCGCCATGCTCTGCTACGTCACGCCCAAGGAGCACCTGGGTCTGCCGAACAAGGATGACGTGAAGACC 
GGGATCATCACCTACAAGATCGCCGCCCATGCCGCCGACCTCGCCAAGGGCCACCCGGGCGCGCAGATCCGC 
GACAACGCGCTGTCCAAGGCGCGCTTCGAGTTCCGTTGGGAGGACCAGTTCAACCTTGGCCTGGACCCGGAC 
ACCGCGCGGGCGTTCCATGACGAGACCCTGCCGAAGGACTCGGCCAAGGTCGCGCACTTCTGCTCCATGTGC 
GGACCGAAGTTCTGCTCGATGAAGATCACCCAGGAAGTCCGCGACTACGCCAAGGAGAACGGCCTGTCCGAC 
GAGAGCAAGGCCATCGAGGCCGGCTTCCAGGAGCAGGCGGCGCGCTTCAAGGACGAAGGATCGGTGATCTAC 
AGGCAGGTGTAG