ViewGene
Locus Name:
GeneID:
Location:

GeneID:3908
TrivialName: PA14_61790
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_61790
PAO1 Ortholog Locus:PA4672
Sequence Length:585
Protein Length:194
Start: 5512065
Stop: 5512649
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08059.1|AE004881_2 (AE004881) peptidyl-tRNA hydrolase [Pseudomonas aeruginosa PAO1]
Identities = 194/194 (100%), Positives = 194/194 (100%)
Sequence:
GTGACTGCCGTACAACTGATCGTTGGCCTGGGAAACCCGGGTCCTGAATACGACCAGACCCGGCATAACGCG
GGGGCCCTTTTCGTTGAGCGCCTGGCGCATGCCCAGGGCGTCAGCCTCGTGGCTGACCGCAAGTATTTCGGC
CTGGTCGGCAAGTTCAGCCACCAGGGCAAGGACGTTCGTCTGTTGATCCCGACTACCTATATGAACCGCAGC
GGCCAGTCCGTGGCGGCGCTGGCGGGATTCTTCCGGATCGCCCCGGACGCCATCCTGGTGGCCCACGACGAA
CTCGACATGCCCCCTGGCGTCGCCAAGCTCAAGACCGGCGGCGGACACGGCGGGCACAACGGGTTGCGCGAT
ATCATCGCCCAGCTCGGCAATCAGAATTCTTTCCATCGCCTGCGGCTTGGCATCGGCCATCCGGGGCACAGC
AGCCTGGTTTCCGGTTACGTGCTCGGCCGCGCCCCGCGCAGCGAGCAGGAACTGCTCGACACCAGCATCGAC
TTCGCCCTCGGCGTGCTGCCGGAAATGCTCGCCGGGGACTGGACCCGGGCGATGCAGAAGCTGCACAGCCAG
AAGGCCTGA
Translation:
MTAVQLIVGLGNPGPEYDQTRHNAGALFVERLAHAQGVSLVADRKYFGLVGKFSHQGKDVRLLIPTTYMNRS
GQSVAALAGFFRIAPDAILVAHDELDMPPGVAKLKTGGGHGGHNGLRDIIAQLGNQNSFHRLRLGIGHPGHS
SLVSGYVLGRAPRSEQELLDTSIDFALGVLPEMLAGDWTRAMQKLHSQKA*
AnnotationID:3915GeneID:3908
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 20:08:57
Gene Name:pth
Confidence Code:2
GeneProduct:peptidyl-tRNA hydrolase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam01195
Secondary Functional Category(ies):
EC Number:3.1.1.29
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66345.1|AE016263_2 (AE016776) peptidyl-tRNA hydrolase [Pseudomonas putida KT2440]
          Length = 194

 Score =  335 bits (860), Expect = 7e-92
 Identities = 164/194 (84%), Positives = 178/194 (91%)

>gb|AAO54631.1| (AE016859) peptidyl-tRNA hydrolase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 194

 Score =  325 bits (834), Expect = 7e-89
 Identities = 156/194 (80%), Positives = 178/194 (91%)

>gb|AAN54254.1|AE015562_4 (AE015562) peptidyl-tRNA hydrolase [Shewanella oneidensis MR-1]
          Length = 195

 Score =  253 bits (647), Expect = 3e-67
 Identities = 126/194 (64%), Positives = 149/194 (76%)






 
Structural Features:
pfam01195, Pept_tRNA_hydro, Peptidyl-tRNA hydrolase. 
CD-Length = 185 residues, 100.0% aligned
Score =  211 bits (538), Expect = 5e-56
Identities = 90/186 (48%), Positives = 122/186 (65%), Gaps = 2/186 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14959




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24961




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36555




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3908_cdd.html


    		




 
URL:

















    ReferenceID:36604




  
Author/Investigator(s):
        Bonin PD, Choi GH, Trepod CM, Mott JE, Lyle SB, Cialdella JI, Sarver RW, Marshall VP, Erickson LA.





  
Title:
        Expression, purification, and characterization of peptidyl-tRNA hydrolase from Staphylococcus aureus.




  
PubMed:
        11812233


MedLine:
        




    
Source:
Protein Expr Purif. 2002 Feb;24(1):123-30.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11812233

















    ReferenceID:36605




  
Author/Investigator(s):
        De La Vega FM, Galindo JM, Old IG, Guarneros G.





  
Title:
        Microbial genes homologous to the peptidyl-tRNA hydrolase-encoding gene of Escherichia coli.




  
PubMed:
        8635758


MedLine:
        




    
Source:
Gene. 1996 Feb 22;169(1):97-100.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8635758

















    ReferenceID:36606




  
Author/Investigator(s):
        Schmitt E, Mechulam Y, Fromant M, Plateau P, Blanquet S.





  
Title:
        Crystal structure at 1.2 A resolution and active site mapping of Escherichia coli peptidyl-tRNA hydrolase.




  
PubMed:
        9303320


MedLine:
        




    
Source:
EMBO J. 1997 Aug 1;16(15):4760-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=97447580&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:3908
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6146
                           PA4672_tr
                           translation of PA4672
                           194
                           99.99
                           99.99
                           0
                           1004.0
     

     
     
170601
                           gb|AAG08059.1|AE004881_2
                           (AE004881) peptidyl-tRNA hydrolase [Pseudomonas aeruginosa PAO1]
                           194
                           99.48
                           99.99
                           0
                           1001.0
     

     
     
170602
                           gb|AAN66345.1|AE016263_2
                           (AE016776) peptidyl-tRNA hydrolase [Pseudomonas putida KT2440]
                           194
                           84.02
                           91.75
                           0
                           856.0
     

     
     
170603
                           gb|AAO54631.1|
                           (AE016859) peptidyl-tRNA hydrolase [Pseudomonas syringae pv. tomato str. DC3000]
                           194
                           79.89
                           91.75
                           0
                           830.0
     

     
     
170604
                           gb|AAN54254.1|AE015562_4
                           (AE015562) peptidyl-tRNA hydrolase [Shewanella oneidensis MR-1]
                           195
                           64.10
                           76.41
                           1
                           643.0
     

     






      

          
GC ORFID: 46910How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4672Glimmer Score: 

          
Start: 4775745Stop: 4776329

          
Length: 585


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4672 translation of PA4672Homolog Bit Score 391.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene5097 (ORF: BLASTX 325.0), t_PA4672 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGACTGCCGTACAACTGATCGTTGGCCTGGGAAACCCGGGTCCTGAATACGACCAGACCCGGCATAACGCG 
GGGGCCCTTTTCGTTGAGCGCCTGGCGCATGCCCAGGGCGTCAGCCTCGTGGCTGACCGCAAGTATTTCGGC 
CTGGTCGGCAAGTTCAGCCACCAGGGCAAGGACGTTCGTCTGTTGATCCCGACTACCTATATGAACCGCAGC 
GGCCAGTCCGTGGCGGCGCTGGCGGGATTCTTCCGGATCGCCCCGGACGCCATCCTGGTGGCCCACGACGAA 
CTCGACATGCCCCCTGGCGTCGCCAAGCTCAAGACCGGCGGCGGACACGGCGGGCACAACGGGTTGCGCGAT 
ATCATCGCCCAGCTCGGCAATCAGAATTCTTTCCATCGCCTGCGGCTTGGCATCGGCCATCCGGGGCACAGC 
AGCCTGGTTTCCGGTTACGTGCTCGGCCGCGCCCCGCGCAGCGAGCAGGAACTGCTCGACACCAGCATCGAC 
TTCGCCCTCGGCGTGCTGCCGGAAATGCTCGCCGGGGACTGGACCCGGGCGATGCAGAAGCTGCACAGCCAG 
AAGGCCTGA