ViewGene
Locus Name:
GeneID:
Location:

GeneID:3889
TrivialName: PA14_72200
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_72200
PAO1 Ortholog Locus:PA5470
Sequence Length:615
Protein Length:204
Start: 6432480
Stop: 6431866
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08855.1|AE004959_11 (AE004959) probable peptide chain release factor [Pseudomonas aeruginosa PAO1]
Identities = 199/204 (97%), Positives = 201/204 (98%)
Sequence:
ATGATCCTGCTGCAACTCTCCGCGGCCCAGGGCCCGGCGGAATGCTGCCTGGCGGTGGCGAAGGCGTTCGAG
CGGCTGTGCCTCGAGGCGGCGCAAGCCGGCGTCGAGGTCGAGGTGCTCGAGGAGGTGGCCGGCGAGCGTCCG
CGCACCTGGCGTTCGCTGCTCCTGGGGCTGCGCGGCATTGCCGCCGAAGCGCTCGCCGAGCACTGGTGCGGC
GGTATCCAGTGGATCTGCACCAGTCCCTACCGGGCGAGGCATGCGCGCAAGAACTGGTTCATCGGCGCGGAG
CGCTTCGCCGCGCCGCCGGCCAGCCTGGAGGGCGAGATCCGTTTCGAGACCCTGCGCTCTTCCGGCCCCGGC
GGCCAGCATGTCAACACCACCGACTCGGCGGTGCGCGCCACCCACCTGGCCAGCGGCATCAGCGTCAGGGTG
CAGAGCCAGCGTAGCCAGCACGCCAACAAACGCCTGGCGATCCTGCTGATCGCCCGGCGCCTCGCGGACCAG
GCGAGCAGCGCGGCCGACGCGCTGCGCGCGGAGCGCCGCCAGGCCCACGGCCGGATCAGTCGCGGCGCGGCT
CGCCGGGTATTCCGCGGCGAGCGTTTCGAGCCGGCCTGA
Translation:
MILLQLSAAQGPAECCLAVAKAFERLCLEAAQAGVEVEVLEEVAGERPRTWRSLLLGLRGIAAEALAEHWCG
GIQWICTSPYRARHARKNWFIGAERFAAPPASLEGEIRFETLRSSGPGGQHVNTTDSAVRATHLASGISVRV
QSQRSQHANKRLAILLIARRLADQASSAADALRAERRQAHGRISRGAARRVFRGERFEPA*
AnnotationID:3896GeneID:3889
AnnotatorUID: diggins
Modification Date/Time: 2005-03-21 06:06:18
Gene Name:
Confidence Code:3
GeneProduct:putative peptide chain release factor
Cell Localization:()
Synonyms:prfH
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1186,pfam00472
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD17850.1| (AL646080) PUTATIVE PEPTIDE CHAIN RELEASE FACTOR HOMOLOG PROTEIN
           [Ralstonia solanacearum]
          Length = 207

 Score =  254 bits (648), Expect = 3e-67
 Identities = 131/204 (64%), Positives = 154/204 (75%)

>gb|AAL19271.1| (AE008709) putative peptide chain release factor [Salmonella
           typhimurium LT2]
          Length = 204

 Score =  237 bits (604), Expect = 4e-62
 Identities = 125/203 (61%), Positives = 143/203 (70%)

>gb|AAN78863.1|AE016756_46 (AE016756) Peptide chain release factor homolog [Escherichia coli
           CFT073]
          Length = 204

 Score =  236 bits (602), Expect = 6e-62
 Identities = 125/201 (62%), Positives = 140/201 (69%)






 
Structural Features:
COG1186, PrfB, Protein chain release factor B 
[Translation, ribosomal structure and biogenesis]
CD-Length = 239 residues,  86.6% aligned
Score =  196 bits (500), Expect = 1e-51
Identities = 64/208 (30%), Positives = 93/208 (44%), Gaps = 6/208 (2%)

pfam00472, RF-1, Peptidyl-tRNA hydrolase domain.
CD-Length = 115 residues,  80.0% aligned
Score = 98.0 bits (244), Expect = 7e-22
Identities = 42/92 (45%), Positives = 55/92 (59%), Gaps = 1/92 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7781




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24923




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34630




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3889_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3889
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6124
                           PA5470_tr
                           translation of PA5470
                           204
                           97.54
                           98.52
                           0
                           1011.0
     

     
     
170319
                           gb|AAG08855.1|AE004959_11
                           (AE004959) probable peptide chain release factor [Pseudomonas aeruginosa PAO1]
                           204
                           97.54
                           98.52
                           0
                           1011.0
     

     
     
170320
                           emb|CAD17850.1|
                           (AL646080) PUTATIVE PEPTIDE CHAIN RELEASE FACTOR HOMOLOG PROTEIN [Ralstonia solanacearum]
                           207
                           63.28
                           74.39
                           3
                           648.0
     

     
     
170321
                           gb|AAL19271.1|
                           (AE008709) putative peptide chain release factor [Salmonella typhimurium LT2]
                           204
                           61.27
                           70.09
                           0
                           603.0
     

     
     
170322
                           gb|AAO70119.1|
                           (AE016842) putative peptide chain release factor [Salmonella enterica subsp. enterica serovar Typhi Ty2]
                           204
                           61.27
                           70.09
                           0
                           603.0
     

     






      

          
GC ORFID: 46891How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5470Glimmer Score: 

          
Start: 5696169Stop: 5695555

          
Length: 615


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5470 translation of PA5470Homolog Bit Score 394.0

          
Other Homologs:  t_PA5470 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCCTGCTGCAACTCTCCGCGGCCCAGGGCCCGGCGGAATGCTGCCTGGCGGTGGCGAAGGCGTTCGAG 
CGGCTGTGCCTCGAGGCGGCGCAAGCCGGCGTCGAGGTCGAGGTGCTCGAGGAGGTGGCCGGCGAGCGTCCG 
CGCACCTGGCGTTCGCTGCTCCTGGGGCTGCGCGGCATTGCCGCCGAAGCGCTCGCCGAGCACTGGTGCGGC 
GGTATCCAGTGGATCTGCACCAGTCCCTACCGGGCGAGGCATGCGCGCAAGAACTGGTTCATCGGCGCGGAG 
CGCTTCGCCGCGCCGCCGGCCAGCCTGGAGGGCGAGATCCGTTTCGAGACCCTGCGCTCTTCCGGCCCCGGC 
GGCCAGCATGTCAACACCACCGACTCGGCGGTGCGCGCCACCCACCTGGCCAGCGGCATCAGCGTCAGGGTG 
CAGAGCCAGCGTAGCCAGCACGCCAACAAACGCCTGGCGATCCTGCTGATCGCCCGGCGCCTCGCGGACCAG 
GCGAGCAGCGCGGCCGACGCGCTGCGCGCGGAGCGCCGCCAGGCCCACGGCCGGATCAGTCGCGGCGCGGCT 
CGCCGGGTATTCCGCGGCGAGCGTTTCGAGCCGGCCTGA