ViewGene
Locus Name:
GeneID:
Location:

GeneID:3878
TrivialName: PA14_12400
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_12400
PAO1 Ortholog Locus:PA3976
Sequence Length:630
Protein Length:209
Start: 1068261
Stop: 1067632
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07363.1|AE004815_7 (AE004815) thiamin-phosphate pyrophosphorylase [Pseudomonas aeruginosa PAO1]
Identities = 207/209 (99%), Positives = 209/209 (100%)
Sequence:
ATGAAACTGCGCGGCCTGTACGCCATCACCGATAGCCAGCTACTGGACGACGGCCGCCTGCTGCCCTACGTC
GAAGCCGCGCTACGCGGCGGCGCGCGCCTGCTGCAATACCGCGACAAATCCAGCGACCAGGCGCGGCGCCTG
CGCGAGGCGGAAAGCCTGCGCGAACTCTGCGAACGCTACGGCGCGCAGTTGATCGTCAACGACGACGCCGAA
CTCGCCGCGCGCCTGGGCGTCGGCCTGCACCTCGGCCAGACCGACGGTTCGCTGTCGGCGGCGCGCGCCCTG
CTCGGCCGCCAGGCGATCATCGGCGCCACCTGCCACGCCCGGCTGGAGCTGGCCGAGCAGGCGGTGGCCGAG
GGCGCCAGCTACGTCGCCTTCGGCCGCTTCTTCAATTCCAGCACCAAACCCGGCGCCCCCGCCGCCAGCGTC
GAACTGCTCGACCAGGCCCGGCCGCGCCTGCACCTGCCGATCACCGCCATCGGCGGCATCAGCCTCGATACC
GCGCCCGGTCTGATCGCCCGCGGCGTCGATCTGGTCGCCGTGATCCATGCGCTGTTCGCCGCCGCGTCCGCC
GCCGAGGTGGAACGCCGCGCCCGCGCCTTCAGCGCCCTGTTCGAACCTGCCTGA
Translation:
MKLRGLYAITDSQLLDDGRLLPYVEAALRGGARLLQYRDKSSDQARRLREAESLRELCERYGAQLIVNDDAE
LAARLGVGLHLGQTDGSLSAARALLGRQAIIGATCHARLELAEQAVAEGASYVAFGRFFNSSTKPGAPAASV
ELLDQARPRLHLPITAIGGISLDTAPGLIARGVDLVAVIHALFAAASAAEVERRARAFSALFEPA*
AnnotationID:3885GeneID:3878
AnnotatorUID: danlee
Modification Date/Time: 2006-01-27 16:04:41
Gene Name:thiE
Confidence Code:2
GeneProduct:thiamin-phosphate pyrophosphorylase
Cell Localization:()
Synonyms:thiC
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:thiamin-phosphate synthase
Functional Category Confidence Code:5
COGs:COG0352,pfam02581
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:
thiamine biosynthesis
Homology:
emb|CAB15855.1| (Z99123) thiamine-phosphate pyrophosphorylase
[Bacillus subtilis subsp. subtilis str. 168]
 Length = 222

Score =  103 bits (257), Expect = 8e-22
Identities = 67/183 (36%), Positives = 97/183 (53%), Gaps = 8/183 (4%)
Structural Features:
gnl|CDD|5488 pfam02581, TMP-TENI, Thiamine monophosphate synthase/TENI. Thiamine
monophosphate synthase (TMP) (EC:2.5.1.3) catalyses the
substitution of the pyrophosphate of 2-methyl-4-amino-5-
hydroxymethylpyrimidine pyrophosphate by 4-methyl-5-
(beta-hydroxyethyl)thiazole phosphate to yield thiamine
phosphate. This Pfam family also includes the regulatory
protein TENI

Length = 202
Score =  136 bits (345), Expect = 3e-33
Identities = 82/198 (41%), Positives = 109/198 (55%), Gaps = 5/198 (2%)
Genomic Context:






   
Comment:


















    ReferenceID:7759




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24901




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34078




  
Author/Investigator(s):
        Zhang Y, Taylor SV, Chiu HJ, Begley TP.





  
Title:
        Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.




  
PubMed:
        9139923


MedLine:
        




    
Source:
J Bacteriol. 1997 May;179(9):3030-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9139923









           
Homologs By Global Alignment 

           

       



     
Gene ID:3878
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6113
                           PA3976_tr
                           translation of PA3976
                           209
                           99.04
                           99.99
                           0
                           1015.0
     

     
     
170185
                           gb|AAG07363.1|AE004815_7
                           (AE004815) thiamin-phosphate pyrophosphorylase [Pseudomonas aeruginosa PAO1]
                           209
                           99.04
                           99.99
                           0
                           1015.0
     

     
     
170186
                           gb|AAN70352.1|AE016677_3
                           (AE016792) thiamine-phosphate pyrophosphorylase [Pseudomonas putida KT2440]
                           209
                           74.64
                           82.77
                           2
                           758.0
     

     
     
170187
                           gb|AAO58228.1|
                           (AE016873) thiamine-phosphate pyrophosphorylase [Pseudomonas syringae pv. tomato str. DC3000]
                           209
                           68.42
                           79.42
                           4
                           718.0
     

     
     
170188
                           gb|AAQ57829.1|
                           (AE016910) probable thiamine-phosphate diphosphorylase [Chromobacterium violaceum ATCC 12472]
                           212
                           49.52
                           59.43
                           8
                           447.0
     

     






      

          
GC ORFID: 46880How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3976Glimmer Score: 

          
Start: 3885677Stop: 3886306

          
Length: 630


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3976 translation of PA3976Homolog Bit Score 395.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5723 (ORF: BLASTX 282.0), t_PA3976 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAACTGCGCGGCCTGTACGCCATCACCGATAGCCAGCTACTGGACGACGGCCGCCTGCTGCCCTACGTC 
GAAGCCGCGCTACGCGGCGGCGCGCGCCTGCTGCAATACCGCGACAAATCCAGCGACCAGGCGCGGCGCCTG 
CGCGAGGCGGAAAGCCTGCGCGAACTCTGCGAACGCTACGGCGCGCAGTTGATCGTCAACGACGACGCCGAA 
CTCGCCGCGCGCCTGGGCGTCGGCCTGCACCTCGGCCAGACCGACGGTTCGCTGTCGGCGGCGCGCGCCCTG 
CTCGGCCGCCAGGCGATCATCGGCGCCACCTGCCACGCCCGGCTGGAGCTGGCCGAGCAGGCGGTGGCCGAG 
GGCGCCAGCTACGTCGCCTTCGGCCGCTTCTTCAATTCCAGCACCAAACCCGGCGCCCCCGCCGCCAGCGTC 
GAACTGCTCGACCAGGCCCGGCCGCGCCTGCACCTGCCGATCACCGCCATCGGCGGCATCAGCCTCGATACC 
GCGCCCGGTCTGATCGCCCGCGGCGTCGATCTGGTCGCCGTGATCCATGCGCTGTTCGCCGCCGCGTCCGCC 
GCCGAGGTGGAACGCCGCGCCCGCGCCTTCAGCGCCCTGTTCGAACCTGCCTGA