ViewGene
Locus Name:
GeneID:
Location:

GeneID:3845
TrivialName: PA14_64670
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_64670
PAO1 Ortholog Locus:PA4893
Sequence Length:615
Protein Length:204
Start: 5759803
Stop: 5760417
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08278.1|AE004902_6 (AE004902) urease accessory protein UreG [Pseudomonas aeruginosa PAO1]
Identities = 204/204 (100%), Positives = 204/204 (100%)
Sequence:
ATGACATCCCAACCCCTGCGTGTCGGCATCGGCGGTCCGGTGGGCTCCGGCAAGACCGCCCTGACCCTGGCC
CTGTGCCGCGAACTGCGCGAGCGCTACAACCTCGCGGTGGTCACCAACGACATCTATACCCAGGAAGACGCA
GAGTTCCTGGTGCGCAACGAGGCCCTCGCCCCCGAGCGGATCATCGGCGTGGAAACCGGCGGCTGCCCGCAC
ACGGCGATCCGCGAGGACGCCTCGATCAACCTGGAGGCGGTGGACCAGCTCAACCGGCGCTTCCCCGGCCTC
GAACTGATCCTGGTGGAATCCGGCGGCGACAACCTGTCGGCCACCTTCAGCCCCGAGCTGTCCGACCTGACC
CTCTACGTGATCGACGTTTCCGCCGGCGACAAGATCCCGCGCAAGGGCGGCCCCGGCATCTGCAAGTCGGAC
CTGCTGGTGATCAACAAGATCGACCTGGCGCCGCTGGTCGGCGCCTCCCTCGACGTGATGGACCGGGACGCC
CGGCGCATGCGCGGCGACAAGCCGTTCGTGTTCAGCAACCAGAAGACCGGCCAGGGGCTGGACGAAATCATC
GCCTTCATCGAGCGCCAGGGCCTGCTGACCGCCGCCTGA
Translation:
MTSQPLRVGIGGPVGSGKTALTLALCRELRERYNLAVVTNDIYTQEDAEFLVRNEALAPERIIGVETGGCPH
TAIREDASINLEAVDQLNRRFPGLELILVESGGDNLSATFSPELSDLTLYVIDVSAGDKIPRKGGPGICKSD
LLVINKIDLAPLVGASLDVMDRDARRMRGDKPFVFSNQKTGQGLDEIIAFIERQGLLTAA*
AnnotationID:3852GeneID:3845
AnnotatorUID: diggins
Modification Date/Time: 2005-03-11 13:01:58
Gene Name:ureG
Confidence Code:2
GeneProduct:urease accessory protein UreG
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0378, pfam01495
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58339.1| (AE016873) urease accessory protein UreG [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 205

 Score =  370 bits (949), Expect = e-102
 Identities = 184/204 (90%), Positives = 197/204 (96%)

>dbj|BAA16973.1| (D90902) urease accessory protein G [Synechocystis sp. PCC 6803]
          Length = 206

 Score =  298 bits (762), Expect = 2e-80
 Identities = 144/196 (73%), Positives = 168/196 (85%)

>dbj|BAC07610.1| (AP005369) urease accessory protein G [Thermosynechococcus
           elongatus BP-1]
          Length = 200

 Score =  295 bits (754), Expect = 2e-79
 Identities = 145/190 (76%), Positives = 166/190 (87%)








 
Structural Features:
COG0378, HypB, Ni2+-binding GTPase involved in regulation of expression and maturation 
of urease and hydrogenase 
[Posttranslational modification, protein turnover, chaperones / Transcription]
CD-Length = 202 residues,  93.6% aligned
Score =  253 bits (647), Expect = 1e-68
Identities = 110/197 (55%), Positives = 139/197 (70%), Gaps = 9/197 (4%)

pfam01495, HypB_UreG, HypB/UreG nucleotide-binding domain.
CD-Length = 130 residues,  91.5% aligned
Score =  157 bits (398), Expect = 1e-39
Identities = 58/127 (45%), Positives = 81/127 (63%), Gaps = 8/127 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7693




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24835




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35140




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3845_cdd.html


    		




 
URL:

















    ReferenceID:35937




  
Author/Investigator(s):
        de Koning-Ward TF, Ward AC, Robins-Browne RM.





  
Title:
        Characterisation of the urease-encoding gene complex of Yersinia enterocolitica.




  
PubMed:
        8045421


MedLine:
        




    
Source:
Gene. 1994 Jul 22;145(1):25-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8045421

















    ReferenceID:35940




  
Author/Investigator(s):
        Sriwanthana B, Island MD, Mobley HL.





  
Title:
        Sequence of the Proteus mirabilis urease accessory gene ureG.




  
PubMed:
        8335248


MedLine:
        




    
Source:
Gene. 1993 Jul 15;129(1):103-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8335248

















    ReferenceID:35941




  
Author/Investigator(s):
        Mulrooney SB, Hausinger RP.





  
Title:
        Sequence of the Klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation.




  
PubMed:
        2211515


MedLine:
        




    
Source:
J Bacteriol. 1990 Oct;172(10):5837-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2211515

















    ReferenceID:35951




  
Author/Investigator(s):
        Maeda M, Hidaka M, Nakamura A, Masaki H, Uozumi T.





  
Title:
        Cloning, sequencing, and expression of thermophilic Bacillus sp. strain TB-90 urease gene complex in Escherichia coli.




  
PubMed:
        8288539


MedLine:
        




    
Source:
J Bacteriol. 1994 Jan;176(2):432-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8288539

















    ReferenceID:35965




  
Author/Investigator(s):
        Zhu J, Teng CH, Chang CF, Chang CD, Simpson KW, Wei C, McDonough P, McDonough S, Chang YF.





  
Title:
        Cloning and characterization of a Helicobacter bizzozeronii urease gene cluster.




  
PubMed:
        12652903


MedLine:
        




    
Source:
DNA Seq. 2002 Dec;13(6):321-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12652903









           
Homologs By Global Alignment 

           

       



     
Gene ID:3845
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6070
                           PA4893_tr
                           translation of PA4893
                           204
                           99.99
                           99.99
                           0
                           1028.0
     

     
     
169368
                           gb|AAG08278.1|AE004902_6
                           (AE004902) urease accessory protein UreG [Pseudomonas aeruginosa PAO1]
                           204
                           99.99
                           99.99
                           0
                           1028.0
     

     
     
169369
                           gb|AAO58339.1|
                           (AE016873) urease accessory protein UreG [Pseudomonas syringae pv. tomato str. DC3000]
                           205
                           89.75
                           96.09
                           1
                           949.0
     

     
     
169372
                           dbj|BAC07610.1|
                           (AP005369) urease accessory protein G [Thermosynechococcus elongatus BP-1]
                           204
                           72.05
                           82.35
                           4
                           743.0
     

     
     
169370
                           dbj|BAA16973.1|
                           (D90902) urease accessory protein G [Synechocystis sp. PCC 6803]
                           207
                           70.53
                           82.12
                           4
                           756.0
     

     






      

          
GC ORFID: 46847How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4893Glimmer Score: 

          
Start: 5023484Stop: 5024098

          
Length: 615


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4893 translation of PA4893Homolog Bit Score 400.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5824 (ORF: BLASTX 372.0), psyr_15may02_Scaffold6_revised_gene6567 (ORF: BLASTX 202.0), t_PA4893 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACATCCCAACCCCTGCGTGTCGGCATCGGCGGTCCGGTGGGCTCCGGCAAGACCGCCCTGACCCTGGCC 
CTGTGCCGCGAACTGCGCGAGCGCTACAACCTCGCGGTGGTCACCAACGACATCTATACCCAGGAAGACGCA 
GAGTTCCTGGTGCGCAACGAGGCCCTCGCCCCCGAGCGGATCATCGGCGTGGAAACCGGCGGCTGCCCGCAC 
ACGGCGATCCGCGAGGACGCCTCGATCAACCTGGAGGCGGTGGACCAGCTCAACCGGCGCTTCCCCGGCCTC 
GAACTGATCCTGGTGGAATCCGGCGGCGACAACCTGTCGGCCACCTTCAGCCCCGAGCTGTCCGACCTGACC 
CTCTACGTGATCGACGTTTCCGCCGGCGACAAGATCCCGCGCAAGGGCGGCCCCGGCATCTGCAAGTCGGAC 
CTGCTGGTGATCAACAAGATCGACCTGGCGCCGCTGGTCGGCGCCTCCCTCGACGTGATGGACCGGGACGCC 
CGGCGCATGCGCGGCGACAAGCCGTTCGTGTTCAGCAACCAGAAGACCGGCCAGGGGCTGGACGAAATCATC 
GCCTTCATCGAGCGCCAGGGCCTGCTGACCGCCGCCTGA