ViewGene
Locus Name:
GeneID:
Location:

GeneID:3841
TrivialName: PA14_47790
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_47790
PAO1 Ortholog Locus:PA1272
Sequence Length:612
Protein Length:203
Start: 4250878
Stop: 4250267
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04661.1|AE004557_2 (AE004557) cob(I)alamin adenosyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 203/203 (100%), Positives = 203/203 (100%)
Sequence:
ATGAACGAATCCCCCGAGAAGGACCAGCGCCACCGCGAGCGGATGGAGCGCAAGAAGGCCGTGGTCGACGAG
AAGATCGCCCAGGCCCGCGACGAACGCGGCGTGCTCCTGGTGCATAGCGGCAACGGCAAGGGCAAGAGCAGC
TCGGCCTTCGGCATGGTCGCGCGGGCCCTGGGCCATGGCATGAAGGTCGGTGTCGTGCAGTTCATCAAGGGC
GCCGCCAGCACCGGCGAGGAAGCCTTCTTCCGGCGCTTCCCCGAGGAAGTCAGCTATCACGTGATGGGCGAG
GGCTTCACCTGGGAGACCCAGGACCGCCAGCGCGACATCGCCAAGGCCGAGGCGGCCTGGAAGGTCGCCGCG
CAATTGCTGGCCGACCCGGACGTTGGCCTGGTGGTGCTGGACGAACTGAACATCGCCCTCAAGCACGGCTAC
CTGGAACTGGACAGGGTCCTCGCCGACATCCAGGCGCGCCCGGCCATGCAGCATGTGGTGGTGACCGGTCGC
GGCGCGCAGCCGGGGATGATCGAGGCCGCCGACACGGTGACCGAGATGAGCCTGGTCAAGCACGCGTTCAAG
GCCGGGATCAAGGCGCAGAAAGGAGTGGAGTTCTGA
Translation:
MNESPEKDQRHRERMERKKAVVDEKIAQARDERGVLLVHSGNGKGKSSSAFGMVARALGHGMKVGVVQFIKG
AASTGEEAFFRRFPEEVSYHVMGEGFTWETQDRQRDIAKAEAAWKVAAQLLADPDVGLVVLDELNIALKHGY
LELDRVLADIQARPAMQHVVVTGRGAQPGMIEAADTVTEMSLVKHAFKAGIKAQKGVEF*
AnnotationID:3848GeneID:3841
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 07:07:52
Gene Name:cobO
Confidence Code:2
GeneProduct:cob(I)alamin adenosyltransferase
Cell Localization:()
Synonyms:btuR
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:ATP:corrinoid adenosyltransferase BtuR/CobO/CobP
Functional Category Confidence Code:5
COGs:COG2109,pfam02572,cd00561
Secondary Functional Category(ies):coenzyme metabolism
EC Number:2.5.1.17
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67293.1|AE016356_1 (AE016780) cob(I)alamin adenosyltransferase [Pseudomonas putida
           KT2440]
          Length = 203

 Score =  311 bits (797), Expect = 2e-84
 Identities = 153/202 (75%), Positives = 178/202 (88%)

>gb|AAO55228.1| (AE016861) cob(I)alamin adenosyltransferase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 203

 Score =  310 bits (793), Expect = 4e-84
 Identities = 148/202 (73%), Positives = 180/202 (89%)

>gb|AAM38035.1| (AE011964) cob(I)alamin adenolsyltransferase [Xanthomonas
           axonopodis pv. citri str. 306]
          Length = 203

 Score =  272 bits (695), Expect = 1e-72
 Identities = 139/200 (69%), Positives = 161/200 (80%), Gaps = 1/200 (0%)






 
Structural Features:
COG2109, BtuR, ATP:corrinoid adenosyltransferase [Coenzyme metabolism]
CD-Length = 198 residues,  99.5% aligned
Score =  269 bits (690), Expect = 1e-73
Identities = 112/197 (56%), Positives = 151/197 (76%)

pfam02572, CobA_CobO_BtuR, ATP:corrinoid adenosyltransferase BtuR/CobO/CobP.
CD-Length = 176 residues, 100.0% aligned
Score =  212 bits (541), Expect = 2e-56
Identities = 96/177 (54%), Positives = 129/177 (72%), Gaps = 1/177 (0%)

cd00561, CobA_CobO_BtuR, ATP:corrinoid adenosyltransferase BtuR/CobO/CobP. 
CD-Length = 159 residues, 100.0% aligned
Score =  194 bits (495), Expect = 5e-51







   
Genomic Context:






   
Comment:


















    ReferenceID:7685




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24827




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37508




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3841_cdd.html


    		




 
URL:

















    ReferenceID:38034




  
Author/Investigator(s):
        Crouzet J, Levy-Schil S, Cameron B, Cauchois L, Rigault S, Rouyez MC, Blanche F, Debussche L, Thibaut D.





  
Title:
        Nucleotide sequence and genetic analysis of a 13.1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and bifunctional cob




  
PubMed:
        1655697


MedLine:
        




    
Source:
J Bacteriol. 1991 Oct;173(19):6074-87.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1655697

















    ReferenceID:38035




  
Author/Investigator(s):
        Lundrigan MD, Kadner RJ.





  
Title:
        Altered cobalamin metabolism in Escherichia coli btuR mutants affects btuB gene regulation.




  
PubMed:
        2644187


MedLine:
        




    
Source:
J Bacteriol. 1989 Jan;171(1):154-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2644187









           
Homologs By Global Alignment 

           

       



     
Gene ID:3841
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6065
                           PA1272_tr
                           translation of PA1272
                           203
                           99.99
                           99.99
                           0
                           1028.0
     

     
     
169247
                           gb|AAG04661.1|AE004557_2
                           (AE004557) cob(I)alamin adenosyltransferase [Pseudomonas aeruginosa PAO1]
                           203
                           99.99
                           99.99
                           0
                           1028.0
     

     
     
169248
                           gb|AAN67293.1|AE016356_1
                           (AE016780) cob(I)alamin adenosyltransferase [Pseudomonas putida KT2440]
                           203
                           75.36
                           87.68
                           0
                           797.0
     

     
     
169249
                           gb|AAO55228.1|
                           (AE016861) cob(I)alamin adenosyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           203
                           72.90
                           88.66
                           0
                           793.0
     

     
     
169250
                           gb|AAM38035.1|
                           (AE011964) cob(I)alamin adenolsyltransferase [Xanthomonas axonopodis pv. citri str. 306]
                           204
                           68.13
                           78.92
                           2
                           694.0
     

     






      

          
GC ORFID: 46843How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1272Glimmer Score: 

          
Start: 703031Stop: 703642

          
Length: 612


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1272 translation of PA1272Homolog Bit Score 400.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6468 (ORF: BLASTX 304.0), t_PA1272 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGAATCCCCCGAGAAGGACCAGCGCCACCGCGAGCGGATGGAGCGCAAGAAGGCCGTGGTCGACGAG 
AAGATCGCCCAGGCCCGCGACGAACGCGGCGTGCTCCTGGTGCATAGCGGCAACGGCAAGGGCAAGAGCAGC 
TCGGCCTTCGGCATGGTCGCGCGGGCCCTGGGCCATGGCATGAAGGTCGGTGTCGTGCAGTTCATCAAGGGC 
GCCGCCAGCACCGGCGAGGAAGCCTTCTTCCGGCGCTTCCCCGAGGAAGTCAGCTATCACGTGATGGGCGAG 
GGCTTCACCTGGGAGACCCAGGACCGCCAGCGCGACATCGCCAAGGCCGAGGCGGCCTGGAAGGTCGCCGCG 
CAATTGCTGGCCGACCCGGACGTTGGCCTGGTGGTGCTGGACGAACTGAACATCGCCCTCAAGCACGGCTAC 
CTGGAACTGGACAGGGTCCTCGCCGACATCCAGGCGCGCCCGGCCATGCAGCATGTGGTGGTGACCGGTCGC 
GGCGCGCAGCCGGGGATGATCGAGGCCGCCGACACGGTGACCGAGATGAGCCTGGTCAAGCACGCGTTCAAG 
GCCGGGATCAAGGCGCAGAAAGGAGTGGAGTTCTGA