ViewGene
Locus Name:
GeneID:
Location:

GeneID:384
TrivialName: PA14_25000
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25000
PAO1 Ortholog Locus:PA3020
Sequence Length:1929
Protein Length:642
Start: 2187393
Stop: 2185465
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06408.1|AE004727_4 (AE004727) probable soluble lytic transglycosylase [Pseudomonas aeruginosa PAO1]
Identities = 638/642 (99%), Positives = 638/642 (99%)
Sequence:
ATGCGCGGTCGCCTGTTCACTCTCGTTTCCTGTCTTTTGCTCAGTATTTCCTCCGTCGACGCGGCCCACGCC
GCTTCCCTGACCGAACAACGCCGTCTCTACGACCAGGCCAAGGCCGCCCTGGCCAAGGGCAACAGCGCGCCC
TACATGGCCTCGCGCAGCGCCCTGCGCGATTACCCGCTGGAACCCTACCTGGCCTACGACGAACTGACCCAT
CGGCTGAAGTCCGCCAGCAACGAGGAGGTCGAGCGCTTCCTCACCGAACACGGCGACCTGCCGCAGATCGGC
TGGCTGAAACTGCGCTGGCTGCGCCTGCTGGCCGACCGCGGCGACTGGAAGACCTTCGTCAACTACTACGAT
CCGAAGCTGAACTTCACCGAACTCGACTGCCTGTATGGCCAGTACCAGCTGGGCCACGGCCAGAAGGCCGAA
GGCTACGCCACCAGCGAACGCCTGTGGCTGGTCGGCAAGTCGCAGCCGGCTGCCTGCGACACCCTGTTCGGC
CTCTGGCAGGGCGAAGGCCAGCTCACCGAGGAAAAGGTCTGGAAACGCCTCAAGCTGGCCGCCGAAGCGCGC
AACTACAGCCTCGCCTCGCACCTCGCGCAGCGCCTGCCGACCCTCGGCAACCAGGGCGCGCTGATGGTCAGC
GTGGCGCAGAACCCCGCCCAGCTGAGCCAGACCGGCCGCTTCAGCCAGCGCGACCATGCCACCGCCGACGTG
GTCGGTCTCGGCCTGCGCCGCCTGGCGCGGCAGGACCCGGAAAAGGCCCTGAGCCTGCTCGACTACTACAGC
TCGGCGCTACCCTTCTCCAGCGACGAGAAGGTCGCCATCGCCCGCGAGATCGGTCTCAGCCTGGCCAAGCGA
TTCGATCCGCGTGCACTGCCGCTGATGACCCAGTACGATCCCGGCCTGCGCGACAACACCGTCACCGAATGG
CGCACCCGCCTGCTGCTGCGCCTGGGCCGCTGGGACGAAGCCTATGCGCTGACCCGCAAGCTGCCCCAGGAT
CTCGCCGCCACCAGCCGCTGGCGCTATTGGCAGGCACGCAGCCTGCAACTGGCACAGCCGAACAGCAAGGAG
CCGATCGCGCTGTACCAGAAGCTCGCCGGCGAGCGCGACTTCTACGGTTTCCTCGCCGCCGACCGCCTGAGC
GTGCCGTACAAGCTCGGCAACCGCCCTGCGCACATCGACCCGCGCGTCCTGCAACGCGTACGCAACGCCGCC
AGCACGCGCCGGGCCATGGAGTTCTTCAACCGTGGCGAAGTGATCAACGCCCGCCGCGAGTGGTACCACGCC
GCGCGCCTGTTCGATCACGACGAGCTGATCGCCCAGGCCAGGCTGGCCTACGACATGCAGTGGTATTTCCCG
GCGATCCGCTCGATCAGCCAGGCGCAGTACTGGGACGACCTGGACATCCGCTTCCCGATGGCGCACCGCGCC
ACCCTGGTGCGCGAAGCGAAGAACCGCGGCCTGCACTCCAGCTGGATCTTCGCCATCACCCGGCAGGAAAGC
GCGTTCATGTCCGATGCCCGTTCCGGAGTCGGCGCCACCGGACTGATGCAGCTGATGCCCGGCACCGCCAAG
GAGACCTCGCGCAAGTTCGGCATCCCGCTGGCCTCGACCCAGCAACTGATCGTGCCCGACGTCAACATCCGC
CTCGGCGCCGCCTACCTGAGCCAGGTGCATGGACAGTTCAACGGCAACCGGGTACTCGCCTCGGCCGCCTAC
AACGCCGGTCCGGGCCGCGTCCGCCAGTGGCTGAAAGATACCCGCCACCTGGCCTTCGACGTGTGGATCGAG
ACCATTCCGTTCGACGAGACTCGCCAGTACGTGCAGAACGTGCTGTCCTATGCGGTGATCTACGGGCAGAAG
CTCAATGCGCCGCAGCCCATCGTCGACTGGCACGAACGCTACTTCGACGATTTCTGA
Translation:
MRGRLFTLVSCLLLSISSVDAAHAASLTEQRRLYDQAKAALAKGNSAPYMASRSALRDYPLEPYLAYDELTH
RLKSASNEEVERFLTEHGDLPQIGWLKLRWLRLLADRGDWKTFVNYYDPKLNFTELDCLYGQYQLGHGQKAE
GYATSERLWLVGKSQPAACDTLFGLWQGEGQLTEEKVWKRLKLAAEARNYSLASHLAQRLPTLGNQGALMVS
VAQNPAQLSQTGRFSQRDHATADVVGLGLRRLARQDPEKALSLLDYYSSALPFSSDEKVAIAREIGLSLAKR
FDPRALPLMTQYDPGLRDNTVTEWRTRLLLRLGRWDEAYALTRKLPQDLAATSRWRYWQARSLQLAQPNSKE
PIALYQKLAGERDFYGFLAADRLSVPYKLGNRPAHIDPRVLQRVRNAASTRRAMEFFNRGEVINARREWYHA
ARLFDHDELIAQARLAYDMQWYFPAIRSISQAQYWDDLDIRFPMAHRATLVREAKNRGLHSSWIFAITRQES
AFMSDARSGVGATGLMQLMPGTAKETSRKFGIPLASTQQLIVPDVNIRLGAAYLSQVHGQFNGNRVLASAAY
NAGPGRVRQWLKDTRHLAFDVWIETIPFDETRQYVQNVLSYAVIYGQKLNAPQPIVDWHERYFDDF*
AnnotationID:393GeneID:384
AnnotatorUID: diggins
Modification Date/Time: 2005-04-11 19:07:11
Gene Name:
Confidence Code:3
GeneProduct:putative soluble lytic transglycosylase
Cell Localization:()
Synonyms:slt
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0741,pfam01464
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67743.1|AE016405_6 (AE016782) soluble lytic transglycosylase, putative [Pseudomonas
           putida KT2440]
          Length = 657

 Score =  945 bits (2442), Expect = 0.0
 Identities = 452/641 (70%), Positives = 537/641 (83%), Gaps = 1/641 (0%)

>gb|AAO56997.1| (AE016868) soluble lytic murein transglycosylase, putative
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 642

 Score =  943 bits (2437), Expect = 0.0
 Identities = 445/641 (69%), Positives = 534/641 (83%)








 
Structural Features:
COG0741, MltE, Soluble lytic murein transglycosylase 
and related regulatory proteins (some contain LysM/invasin domains) 
[Cell envelope biogenesis, outer membrane]
CD-Length = 296 residues,  99.7% aligned
Score =  129 bits (323), Expect = 1e-30
Identities = 86/298 (28%), Positives = 116/298 (38%), Gaps = 8/298 (2%)

pfam01464, SLT, Transglycosylase SLT domain.
CD-Length = 117 residues,  95.7% aligned
Score = 95.4 bits (237), Expect = 2e-20
Identities = 50/115 (43%), Positives = 67/115 (58%), Gaps = 3/115 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:771




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17913




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36031




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:384_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:384
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
642
                           PA3020_tr
                           translation of PA3020
                           642
                           99.37
                           99.37
                           0
                           3329.0
     

     
     
29575
                           gb|AAG06408.1|AE004727_4
                           (AE004727) probable soluble lytic transglycosylase [Pseudomonas aeruginosa PAO1]
                           642
                           99.37
                           99.37
                           0
                           3329.0
     

     
     
29577
                           gb|AAO56997.1|
                           (AE016868) soluble lytic murein transglycosylase, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           642
                           69.31
                           83.17
                           0
                           2437.0
     

     
     
29576
                           gb|AAN67743.1|AE016405_6
                           (AE016782) soluble lytic transglycosylase, putative [Pseudomonas putida KT2440]
                           658
                           68.69
                           81.61
                           17
                           2444.0
     

     
     
29580
                           emb|CAC45637.1|
                           (AL591786) PUTATIVE MUREIN TRANSGLYCOSYLASE PROTEIN [Sinorhizobium meliloti]
                           783
                           22.73
                           34.48
                           243
                           292.0
     

     






      

          
GC ORFID: 43386How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3020Glimmer Score: 

          
Start: 2766534Stop: 2768462

          
Length: 1929


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3020 translation of PA3020Homolog Bit Score 1286.0

          
Other Homologs:  psyr_15may02_Scaffold9_revised_gene8146 (ORF: BLASTX 945.0), t_PA3020 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCGGTCGCCTGTTCACTCTCGTTTCCTGTCTTTTGCTCAGTATTTCCTCCGTCGACGCGGCCCACGCC 
GCTTCCCTGACCGAACAACGCCGTCTCTACGACCAGGCCAAGGCCGCCCTGGCCAAGGGCAACAGCGCGCCC 
TACATGGCCTCGCGCAGCGCCCTGCGCGATTACCCGCTGGAACCCTACCTGGCCTACGACGAACTGACCCAT 
CGGCTGAAGTCCGCCAGCAACGAGGAGGTCGAGCGCTTCCTCACCGAACACGGCGACCTGCCGCAGATCGGC 
TGGCTGAAACTGCGCTGGCTGCGCCTGCTGGCCGACCGCGGCGACTGGAAGACCTTCGTCAACTACTACGAT 
CCGAAGCTGAACTTCACCGAACTCGACTGCCTGTATGGCCAGTACCAGCTGGGCCACGGCCAGAAGGCCGAA 
GGCTACGCCACCAGCGAACGCCTGTGGCTGGTCGGCAAGTCGCAGCCGGCTGCCTGCGACACCCTGTTCGGC 
CTCTGGCAGGGCGAAGGCCAGCTCACCGAGGAAAAGGTCTGGAAACGCCTCAAGCTGGCCGCCGAAGCGCGC 
AACTACAGCCTCGCCTCGCACCTCGCGCAGCGCCTGCCGACCCTCGGCAACCAGGGCGCGCTGATGGTCAGC 
GTGGCGCAGAACCCCGCCCAGCTGAGCCAGACCGGCCGCTTCAGCCAGCGCGACCATGCCACCGCCGACGTG 
GTCGGTCTCGGCCTGCGCCGCCTGGCGCGGCAGGACCCGGAAAAGGCCCTGAGCCTGCTCGACTACTACAGC 
TCGGCGCTACCCTTCTCCAGCGACGAGAAGGTCGCCATCGCCCGCGAGATCGGTCTCAGCCTGGCCAAGCGA 
TTCGATCCGCGTGCACTGCCGCTGATGACCCAGTACGATCCCGGCCTGCGCGACAACACCGTCACCGAATGG 
CGCACCCGCCTGCTGCTGCGCCTGGGCCGCTGGGACGAAGCCTATGCGCTGACCCGCAAGCTGCCCCAGGAT 
CTCGCCGCCACCAGCCGCTGGCGCTATTGGCAGGCACGCAGCCTGCAACTGGCACAGCCGAACAGCAAGGAG 
CCGATCGCGCTGTACCAGAAGCTCGCCGGCGAGCGCGACTTCTACGGTTTCCTCGCCGCCGACCGCCTGAGC 
GTGCCGTACAAGCTCGGCAACCGCCCTGCGCACATCGACCCGCGCGTCCTGCAACGCGTACGCAACGCCGCC 
AGCACGCGCCGGGCCATGGAGTTCTTCAACCGTGGCGAAGTGATCAACGCCCGCCGCGAGTGGTACCACGCC 
GCGCGCCTGTTCGATCACGACGAGCTGATCGCCCAGGCCAGGCTGGCCTACGACATGCAGTGGTATTTCCCG 
GCGATCCGCTCGATCAGCCAGGCGCAGTACTGGGACGACCTGGACATCCGCTTCCCGATGGCGCACCGCGCC 
ACCCTGGTGCGCGAAGCGAAGAACCGCGGCCTGCACTCCAGCTGGATCTTCGCCATCACCCGGCAGGAAAGC 
GCGTTCATGTCCGATGCCCGTTCCGGAGTCGGCGCCACCGGACTGATGCAGCTGATGCCCGGCACCGCCAAG 
GAGACCTCGCGCAAGTTCGGCATCCCGCTGGCCTCGACCCAGCAACTGATCGTGCCCGACGTCAACATCCGC 
CTCGGCGCCGCCTACCTGAGCCAGGTGCATGGACAGTTCAACGGCAACCGGGTACTCGCCTCGGCCGCCTAC 
AACGCCGGTCCGGGCCGCGTCCGCCAGTGGCTGAAAGATACCCGCCACCTGGCCTTCGACGTGTGGATCGAG 
ACCATTCCGTTCGACGAGACTCGCCAGTACGTGCAGAACGTGCTGTCCTATGCGGTGATCTACGGGCAGAAG 
CTCAATGCGCCGCAGCCCATCGTCGACTGGCACGAACGCTACTTCGACGATTTCTGA