ViewGene
Locus Name:
GeneID:
Location:

GeneID:3832
TrivialName: PA14_08340
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_08340
PAO1 Ortholog Locus:PA0649
Sequence Length:606
Protein Length:201
Start: 714461
Stop: 715066
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04038.1|AE004500_4 (AE004500) anthranilate synthase component II [Pseudomonas aeruginosa PAO1]
Identities = 199/201 (99%), Positives = 201/201 (100%)
Sequence:
ATGCTGCTGATGATCGATAACTACGACTCCTTCACCTACAACCTGGTGCAGTACTTCGGCGAACTCAAGGCC
GAGGTCAAGGTGGTGCGCAACGACGAACTGAGCGTGGAACAGATCGAGGCCCTGGCGCCCGAGCGCATCGTC
CTTTCCCCCGGTCCCTGCACCCCCAACGAGGCCGGCGTCTCGCTGGCCGTTATCGAGCGCTTCGCCGGCAAG
CTGCCGCTGCTCGGTGTCTGCCTTGGCCATCAGAGCATCGGCCAGGCCTTCGGCGGCGAAGTGGTGCGGGCG
CGGCAGGTGATGCACGGCAAGACCAGCCCGATCCACCACAAGAACCTCGGCGTGTTCGCCGGCCTGGCCAAT
CCCCTGACGGTGACGCGCTACCACTCGCTGGTGGTGAAGCGAGAGAGCCTGCCGGAGTGCCTGGAGATCACC
GCCTGGACCCAACATGCCGACGGATCGCTCGACGAGATCATGGGTGTACGCCACAAGACCCTGAATGTCGAG
GGCGTGCAGTTCCATCCCGAGTCCATCCTCACCGAGCAGGGCCACGAGTTGCTGGCAAACTTCCTCCGCCAG
CAGGGCGGTGTGCGTGGGGAGGGCAACTGA
Translation:
MLLMIDNYDSFTYNLVQYFGELKAEVKVVRNDELSVEQIEALAPERIVLSPGPCTPNEAGVSLAVIERFAGK
LPLLGVCLGHQSIGQAFGGEVVRARQVMHGKTSPIHHKNLGVFAGLANPLTVTRYHSLVVKRESLPECLEIT
AWTQHADGSLDEIMGVRHKTLNVEGVQFHPESILTEQGHELLANFLRQQGGVRGEGN*
AnnotationID:3839GeneID:3832
AnnotatorUID: diggins
Modification Date/Time: 2005-03-29 14:02:01
Gene Name:trpG
Confidence Code:1
GeneProduct:anthranilate synthase component II
Cell Localization:()
Synonyms:pabA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:Anthranilate/para-aminobenzoate synthases component II
Functional Category Confidence Code:5
COGs:COG0512,pfam00117
Secondary Functional Category(ies):biosynthesis of cofactors, prosthetic groups and carriers
EC Number:4.1.3.27
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66050.1|AE016234_3 (AE016775) anthranilate synthase, component II [Pseudomonas putida
           KT2440]
          Length = 197

 Score =  343 bits (880), Expect = 4e-94
 Identities = 162/197 (82%), Positives = 183/197 (92%)

>gb|AAO54134.1| (AE016858) anthranilate synthase, component II [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 199

 Score =  333 bits (855), Expect = 3e-91
 Identities = 161/197 (81%), Positives = 180/197 (91%)

>gb|AAF95760.1| (AE004328) para-aminobenzoate synthase glutamine amidotransferase,
           component II [Vibrio cholerae]
          Length = 193

 Score =  305 bits (782), Expect = 8e-83
 Identities = 145/192 (75%), Positives = 168/192 (87%)








 
Structural Features:
COG0512, PabA, Anthranilate/para-aminobenzoate synthases component II 
[Amino acid transport and metabolism / Coenzyme metabolism]
CD-Length = 191 residues,  98.4% aligned
Score =  308 bits (790), Expect = 3e-85
Identities = 127/192 (66%), Positives = 151/192 (78%), Gaps = 4/192 (2%)

pfam00117, GATase, Glutamine amidotransferase class-I.
CD-Length = 188 residues,  99.5% aligned
Score =  200 bits (510), Expect = 8e-53
Identities = 94/193 (48%), Positives = 122/193 (63%), Gaps = 9/193 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7667




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24809




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34605




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3832_cdd.html


    		




 
URL:

















    ReferenceID:35253




  
Author/Investigator(s):
        Crawford IP, Eberly L.





  
Title:
        Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: I. Sequence of trpG encoding the glutamine amidotransferase subunit.




  
PubMed:
        3127654


MedLine:
        




    
Source:
Mol Biol Evol. 1986 Sep;3(5):436-48.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3127654

















    ReferenceID:35254




  
Author/Investigator(s):
        Crawford IP, Wilde A, Yelverton EM, Figurski D, Hedges RW.





  
Title:
        Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: II. Cloning and expression in Escherichia coli.




  
PubMed:
        3127655


MedLine:
        




    
Source:
Mol Biol Evol. 1986 Sep;3(5):449-58.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3127655

















    ReferenceID:35255




  
Author/Investigator(s):
        Essar DW, Eberly L, Hadero A, Crawford IP.





  
Title:
        Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications.




  
PubMed:
        2153661


MedLine:
        




    
Source:
J Bacteriol. 1990 Feb;172(2):884-900.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2153661

















    ReferenceID:35256




  
Author/Investigator(s):
        Essar DW, Eberly L, Han CY, Crawford IP.





  
Title:
        DNA sequences and characterization of four early genes of the tryptophan pathway in Pseudomonas aeruginosa.




  
PubMed:
        2105306


MedLine:
        




    
Source:
J Bacteriol. 1990 Feb;172(2):853-66.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2105306









           
Homologs By Global Alignment 

           

       



     
Gene ID:3832
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6054
                           PA0649_tr
                           translation of PA0649
                           201
                           99.00
                           99.99
                           0
                           1035.0
     

     
     
168664
                           gb|AAG04038.1|AE004500_4
                           (AE004500) anthranilate synthase component II [Pseudomonas aeruginosa PAO1]
                           201
                           99.00
                           99.99
                           0
                           1035.0
     

     
     
168665
                           gb|AAA25823.1|
                           (M33814) trpG protein [Pseudomonas aeruginosa]
                           201
                           98.50
                           99.99
                           0
                           1034.0
     

     
     
168666
                           gb|AAN66050.1|AE016234_3
                           (AE016775) anthranilate synthase, component II [Pseudomonas putida KT2440]
                           201
                           80.59
                           91.04
                           4
                           880.0
     

     
     
168668
                           gb|AAO54134.1|
                           (AE016858) anthranilate synthase, component II [Pseudomonas syringae pv. tomato str. DC3000]
                           201
                           80.59
                           90.54
                           2
                           853.0
     

     






      

          
GC ORFID: 46834How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0649Glimmer Score: 

          
Start: 6515804Stop: 6516409

          
Length: 606


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0649 translation of PA0649Homolog Bit Score 403.0

          
Other Homologs:  psyr_15may02_Scaffold14_revised_gene2357 (ORF: BLASTX 328.0), t_PA1002 (ORF: BLASTX 141.0), t_PA0649 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTGCTGATGATCGATAACTACGACTCCTTCACCTACAACCTGGTGCAGTACTTCGGCGAACTCAAGGCC 
GAGGTCAAGGTGGTGCGCAACGACGAACTGAGCGTGGAACAGATCGAGGCCCTGGCGCCCGAGCGCATCGTC 
CTTTCCCCCGGTCCCTGCACCCCCAACGAGGCCGGCGTCTCGCTGGCCGTTATCGAGCGCTTCGCCGGCAAG 
CTGCCGCTGCTCGGTGTCTGCCTTGGCCATCAGAGCATCGGCCAGGCCTTCGGCGGCGAAGTGGTGCGGGCG 
CGGCAGGTGATGCACGGCAAGACCAGCCCGATCCACCACAAGAACCTCGGCGTGTTCGCCGGCCTGGCCAAT 
CCCCTGACGGTGACGCGCTACCACTCGCTGGTGGTGAAGCGAGAGAGCCTGCCGGAGTGCCTGGAGATCACC 
GCCTGGACCCAACATGCCGACGGATCGCTCGACGAGATCATGGGTGTACGCCACAAGACCCTGAATGTCGAG 
GGCGTGCAGTTCCATCCCGAGTCCATCCTCACCGAGCAGGGCCACGAGTTGCTGGCAAACTTCCTCCGCCAG 
CAGGGCGGTGTGCGTGGGGAGGGCAACTGA