ViewGene
Locus Name:
GeneID:
Location:

GeneID:3828
TrivialName: PA14_45940
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45940
PAO1 Ortholog Locus:PA1432
Sequence Length:606
Protein Length:201
Start: 4084975
Stop: 4084370
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04821.1|AE004572_9 (AE004572) autoinducer synthesis protein LasI [Pseudomonas aeruginosa PAO1]
Identities = 201/201 (100%), Positives = 201/201 (100%)
Sequence:
ATGATCGTACAAATTGGTCGGCGCGAAGAGTTCGATAAAAAACTGCTGGGCGAGATGCACAAGTTGCGTGCT
CAAGTGTTCAAGGAGCGCAAAGGCTGGGACGTTAGTGTCATCGACGAGATGGAAATCGATGGTTATGACGCA
CTCAGTCCTTATTACATGTTGATCCAGGAAGATACTCCTGAAGCCCAGGTTTTCGGTTGCTGGCGAATTCTC
GATACCACTGGCCCCTACATGCTGAAGAACACCTTCCCGGAGCTTCTGCACGGCAAGGAAGCGCCTTGCTCG
CCGCACATCTGGGAACTCAGCCGTTTCGCCATCAACTCTGGACAGAAAGGCTCGCTGGGCTTTTCCGACTGT
ACGCTGGAGGCGATGCGCGCGCTGGCCCGCTACAGCCTGCAGAACGACATCCAGACGCTGGTGACGGTAACC
ACCGTAGGGGTGGAGAAGATGATGATCCGTGCCGGCCTGGACGTATCGCGCTTCGGTCCGCACCTGAAGATC
GGCATCGAGCGCGCGGTGGCCTTGCGCATCGAACTCAATGCCAAGACCCAGATCGCGCTTTACGGGGGAGTG
CTGGTGGAACAGCGACTGGCGGTTTCATGA
Translation:
MIVQIGRREEFDKKLLGEMHKLRAQVFKERKGWDVSVIDEMEIDGYDALSPYYMLIQEDTPEAQVFGCWRIL
DTTGPYMLKNTFPELLHGKEAPCSPHIWELSRFAINSGQKGSLGFSDCTLEAMRALARYSLQNDIQTLVTVT
TVGVEKMMIRAGLDVSRFGPHLKIGIERAVALRIELNAKTQIALYGGVLVEQRLAVS*
AnnotationID:3835GeneID:3828
AnnotatorUID: diggins
Modification Date/Time: 2005-05-27 08:08:16
Gene Name:lasI
Confidence Code:1
GeneProduct:autoinducer synthesis protein LasI
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(1) Adaptation, protection
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3916,pfam00765
Secondary Functional Category(ies):signal transduction mechanisms
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAM75411.1| (AY115588) AHL synthase PpuI [Pseudomonas putida]
          Length = 197

 Score =  230 bits (586), Expect = 4e-60
 Identities = 113/192 (58%), Positives = 137/192 (71%)

>gb|AAK28505.1| (AF318063) MupI [Pseudomonas fluorescens]
          Length = 191

 Score =  209 bits (532), Expect = 8e-54
 Identities = 98/180 (54%), Positives = 137/180 (76%)






 
Structural Features:
COG3916, LasI, N-acyl-L-homoserine lactone synthetase 
[Signal transduction mechanisms / 
Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 209 residues,  92.3% aligned
Score =  227 bits (579), Expect = 1e-60
Identities = 89/196 (45%), Positives = 120/196 (61%), Gaps = 7/196 (3%)

pfam00765, Autoind_synth, Autoinducer synthetase.
CD-Length = 182 residues,  95.6% aligned
Score =  107 bits (268), Expect = 1e-24
Identities = 43/177 (24%), Positives = 80/177 (45%), Gaps = 5/177 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7659




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24801




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37362




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3828_cdd.html


    		




 
URL:

















    ReferenceID:37991




  
Author/Investigator(s):
        Passador L, Cook JM, Gambello MJ, Rust L, Iglewski BH.





  
Title:
        Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication.




  
PubMed:
        8493556


MedLine:
        




    
Source:
Science. 1993 May 21;260(5111):1127-30.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=93262462&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:3828
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6050
                           PA1432_tr
                           translation of PA1432
                           201
                           99.99
                           99.99
                           0
                           1036.0
     

     
     
168590
                           gb|AAG04821.1|AE004572_9
                           (AE004572) autoinducer synthesis protein LasI [Pseudomonas aeruginosa PAO1]
                           201
                           99.99
                           99.99
                           0
                           1036.0
     

     
     
168591
                           gb|AAA25875.1|
                           (L04681) virulence protein [Pseudomonas aeruginosa]
                           201
                           99.99
                           99.99
                           0
                           1036.0
     

     
     
168592
                           gb|AAM75411.1|
                           (AY115588) AHL synthase PpuI [Pseudomonas putida]
                           201
                           56.21
                           68.15
                           4
                           578.0
     

     
     
168593
                           gb|AAK28505.1|
                           (AF318063) MupI [Pseudomonas fluorescens]
                           201
                           49.75
                           69.65
                           10
                           525.0
     

     






      

          
GC ORFID: 46830How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1432Glimmer Score: 

          
Start: 868934Stop: 869539

          
Length: 606


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1432 translation of PA1432Homolog Bit Score 403.0

          
Other Homologs:  t_PA1432 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCGTACAAATTGGTCGGCGCGAAGAGTTCGATAAAAAACTGCTGGGCGAGATGCACAAGTTGCGTGCT 
CAAGTGTTCAAGGAGCGCAAAGGCTGGGACGTTAGTGTCATCGACGAGATGGAAATCGATGGTTATGACGCA 
CTCAGTCCTTATTACATGTTGATCCAGGAAGATACTCCTGAAGCCCAGGTTTTCGGTTGCTGGCGAATTCTC 
GATACCACTGGCCCCTACATGCTGAAGAACACCTTCCCGGAGCTTCTGCACGGCAAGGAAGCGCCTTGCTCG 
CCGCACATCTGGGAACTCAGCCGTTTCGCCATCAACTCTGGACAGAAAGGCTCGCTGGGCTTTTCCGACTGT 
ACGCTGGAGGCGATGCGCGCGCTGGCCCGCTACAGCCTGCAGAACGACATCCAGACGCTGGTGACGGTAACC 
ACCGTAGGGGTGGAGAAGATGATGATCCGTGCCGGCCTGGACGTATCGCGCTTCGGTCCGCACCTGAAGATC 
GGCATCGAGCGCGCGGTGGCCTTGCGCATCGAACTCAATGCCAAGACCCAGATCGCGCTTTACGGGGGAGTG 
CTGGTGGAACAGCGACTGGCGGTTTCATGA