ViewGene
Locus Name:
GeneID:
Location:

GeneID:3826
TrivialName: PA14_46580
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_46580
PAO1 Ortholog Locus:
Sequence Length:1116
Protein Length:371
Start: 4146826
Stop: 4147941
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAO54363.1| (AE016858) efflux transporter, RND family, MFP subunit [Pseudomonas syringae pv. tomato str. DC3000]
Identities = 258/371 (69%), Positives = 308/371 (83%)
Sequence:
ATGCGCCGGCGCAACCTCCTTCTGTCCCCCCTGGTCGGTGTAATTCCGTTCGTGCTGCTCGCCTGTAGCGAC
CAGACGCCCCCCGATCCCCGCACTGACGCGCCACTGGTTCGCATCACCACCATCCAGTCGGCAACCGCTACA
TCGCGCGCCTTCACCGGCATCGTCGCGGCCAGGGTGCAGAGCGACCTGGGTTTCCGCGTGTCCGGCAAGGTG
TTGCAGCGCCTGGTCGATACCGGCCAGACCGTCAAGCGTGGCCAGGTGCTTTTGCGCCTCGATCCTATCGAC
CTGCAACTCGCCGCCCATGCCCAGCGCGAGGCGGTGACCGCAGCGCGGGCACGGGCTCAGCAGGCCAGCGAT
GACGAAGCTCGTTACCGCGCCCTGCGCGGCACTGGCGCGGTATCGGCATCTGCCTACGACCAATACAAGGCA
GCGGCGGATGCGGCTCGCGCGCAACTCAGTGCAGCCGAGGCCCAAGCCAAGGTTGCCGGTAACGCCACCCGC
TACGCCGAGCTGCTCGCCGATGCCGACGGTATCGTCATGGAGACACTGGCCGAGCCAGGCCAGGTGGTCAGC
GCCGGCCAGGCCGTGGTACGGCTTGCACATGCCGGCCCACGCGAAGCCCTGGTGCAACTGCCGGAAACCCTG
CGTCCAGCAGTCGGTTCCAGCGCTGAGGCCAAGCTCTTCGGCAGGCAAGACGTCAGCGTCGCCACCCGACTG
CGCCAGCTGTCGGATGTCGCCGACCGCCAGACCCGCACTTTCGAGGCGCGTTACGTGCTCGAAGGCGAGCTG
TCCGACGCGCCGCTGGGCACCACCATCACCGTGCAAATCAGCGATCCGCAAACTGCTTCACCAAGCACAGTG
CAGGTGCCCATCGCCGCGCTGTACGACGCAGGCAACGGGCCTGGCATCTGGATGATTCGCGGAGAGCCCGTA
GAGGTGGCGTGGACGCCCGTAAACGTTCAGTCGCTGGATGATGAGAGCGCCCGCATCAGCGGCGCGATCAAG
CCGGGCGACCAGATCGTCGCCCTGGGCGCGCACCTGCTGCACGACGGCCAGGCCGTTCGCCTGGCCGAGCCG
GCCACCGTGGCGGCAGTCGAGAAAGGCCAGCCATGA
Translation:
MRRRNLLLSPLVGVIPFVLLACSDQTPPDPRTDAPLVRITTIQSATATSRAFTGIVAARVQSDLGFRVSGKV
LQRLVDTGQTVKRGQVLLRLDPIDLQLAAHAQREAVTAARARAQQASDDEARYRALRGTGAVSASAYDQYKA
AADAARAQLSAAEAQAKVAGNATRYAELLADADGIVMETLAEPGQVVSAGQAVVRLAHAGPREALVQLPETL
RPAVGSSAEAKLFGRQDVSVATRLRQLSDVADRQTRTFEARYVLEGELSDAPLGTTITVQISDPQTASPSTV
QVPIAALYDAGNGPGIWMIRGEPVEVAWTPVNVQSLDDESARISGAIKPGDQIVALGAHLLHDGQAVRLAEP
ATVAAVEKGQP*
AnnotationID:3833GeneID:3826
AnnotatorUID: danlee
Modification Date/Time: 2005-09-28 14:02:13
Gene Name:
Confidence Code:3
GeneProduct:putative multidrug efflux gene product
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(14) Hypothetical, unclassified, unknown
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0845
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
Similarity to putative efflux transporter, RND family, MFP subunit [Pseudomonas syringae
pv. tomato str. DC3000] (gi|28868049|ref|NP_790668.1|)
and Secretion protein HlyD [Burkholderia ambifaria AMMD] (gi|74017893|ref|ZP_00688515.1|).






 
Structural Features:
COG0845, AcrA, Membrane-fusion protein [Cell envelope biogenesis, outer membrane].
             CD-Length = 372 residues,  98.1% aligned
             Score = 65.1 bits (157), Expect = 1e-11






   
Genomic Context:
Upstream of putative AcrB/AcrD/AcrF gene product (sometimes involved in drug resistance).






   
Comment:


















    ReferenceID:7655




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24797




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:3826
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
168397
                           gb|AAO54363.1|
                           (AE016858) efflux transporter, RND family, MFP subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           371
                           69.54
                           83.01
                           0
                           1324.0
     

     
     
168399
                           gb|AAM39739.1|
                           (AE012139) component of multidrug efflux system [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           375
                           64.80
                           78.93
                           8
                           1204.0
     

     
     
168398
                           emb|CAD16994.1|
                           (AL646074) PROBABLE TRANSPORT LIPOPROTEIN [Ralstonia solanacearum]
                           374
                           64.17
                           79.14
                           6
                           1224.0
     

     
     
168400
                           gb|AAM35329.1|
                           (AE011670) component of multidrug efflux system [Xanthomonas axonopodis pv. citri str. 306]
                           373
                           62.73
                           76.13
                           10
                           1137.0
     

     
     
168401
                           gb|AAN68908.1|AE016522_5
                           (AE016786) RND efflux membrane fusion protein [Pseudomonas putida KT2440]
                           373
                           62.46
                           75.06
                           8
                           1101.0
     

     






      

          
GC ORFID: 46828How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: psyr_15may02_Scaffold11_revised_gene1943Glimmer Score: 

          
Start: 807083Stop: 805968

          
Length: 1116


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: psyr_15may02_Scaffold11_revised_gene1943 related to: gi|15838675|ref|NP_299363.1| (NC_002488) component of multidrug efflux system [Xylella fastidiosa 9a5c] pir||C82604 component of multidrug efflux system XF2084 [imported] - Xylella fastidiosa (strain 9aHomolog Bit Score 404.0

          
Other Homologs:  psyr_15may02_Scaffold11_revised_gene1943 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCCGGCGCAACCTCCTTCTGTCCCCCCTGGTCGGTGTAATTCCGTTCGTGCTGCTCGCCTGTAGCGAC 
CAGACGCCCCCCGATCCCCGCACTGACGCGCCACTGGTTCGCATCACCACCATCCAGTCGGCAACCGCTACA 
TCGCGCGCCTTCACCGGCATCGTCGCGGCCAGGGTGCAGAGCGACCTGGGTTTCCGCGTGTCCGGCAAGGTG 
TTGCAGCGCCTGGTCGATACCGGCCAGACCGTCAAGCGTGGCCAGGTGCTTTTGCGCCTCGATCCTATCGAC 
CTGCAACTCGCCGCCCATGCCCAGCGCGAGGCGGTGACCGCAGCGCGGGCACGGGCTCAGCAGGCCAGCGAT 
GACGAAGCTCGTTACCGCGCCCTGCGCGGCACTGGCGCGGTATCGGCATCTGCCTACGACCAATACAAGGCA 
GCGGCGGATGCGGCTCGCGCGCAACTCAGTGCAGCCGAGGCCCAAGCCAAGGTTGCCGGTAACGCCACCCGC 
TACGCCGAGCTGCTCGCCGATGCCGACGGTATCGTCATGGAGACACTGGCCGAGCCAGGCCAGGTGGTCAGC 
GCCGGCCAGGCCGTGGTACGGCTTGCACATGCCGGCCCACGCGAAGCCCTGGTGCAACTGCCGGAAACCCTG 
CGTCCAGCAGTCGGTTCCAGCGCTGAGGCCAAGCTCTTCGGCAGGCAAGACGTCAGCGTCGCCACCCGACTG 
CGCCAGCTGTCGGATGTCGCCGACCGCCAGACCCGCACTTTCGAGGCGCGTTACGTGCTCGAAGGCGAGCTG 
TCCGACGCGCCGCTGGGCACCACCATCACCGTGCAAATCAGCGATCCGCAAACTGCTTCACCAAGCACAGTG 
CAGGTGCCCATCGCCGCGCTGTACGACGCAGGCAACGGGCCTGGCATCTGGATGATTCGCGGAGAGCCCGTA 
GAGGTGGCGTGGACGCCCGTAAACGTTCAGTCGCTGGATGATGAGAGCGCCCGCATCAGCGGCGCGATCAAG 
CCGGGCGACCAGATCGTCGCCCTGGGCGCGCACCTGCTGCACGACGGCCAGGCCGTTCGCCTGGCCGAGCCG 
GCCACCGTGGCGGCAGTCGAGAAAGGCCAGCCATGA