ViewGene
Locus Name:
GeneID:
Location:

GeneID:3819
TrivialName: PA14_19130
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_19130
PAO1 Ortholog Locus:PA3476
Sequence Length:606
Protein Length:201
Start: 1651896
Stop: 1652501
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAA82725.1| (U11811) putative autoinducer synthetase [Pseudomonas aeruginosa]
Identities = 199/201 (99%), Positives = 200/201 (99%)
Sequence:
ATGATCGAATTGCTCTCTGAATCGCTGGAAGGGCTTTCCGCCGCCATGATCGCCGAGCTGGGACGCTACCGG
CATCAAGTCTTCATCGAGAAGCTGGGCTGGGACGTGGTCTCCACCTCCAGGGTCCGCGACCAGGAATTCGAC
CAGTTCGACCATCCGCAAACCCGCTACATCGTCGCCATGGGCCGCCAGGGCATCTGCGGTTGCGCCCGCCTG
CTGCCGACGACCGACGCCTACCTGCTCAAGGACGTCTTCGCCTACCTGTGCAGCGAAACCCCGCCGAGCGAT
CCGTCGGTCTGGGAGCTTTCGCGCTACGCCGCCAGCGCGGCGGACGATCCGCAGCTGGCGATGAAGATATTC
TGGTCCAGCCTGCAATGCGGCTGGTACCTGGGGGCCAGTTCGGTGGTGGCGGTGACCACCACGGCCATGGAG
CGCTATTTCGTTCGCAACGGCGTGATCCTCCAGCGCCTCGGCCCGCCGCAGAAGGTCAAGGGCGAGACGCTG
GTCGCGATCAGCTTCCCGGCCTACCAGGAGCGCGGCCTGGAGATGCTGTTGCGCTACCACCCGGAATGGCTG
CAGGGTGTACCGCTGTCGATGGCGGTGTGA
Translation:
MIELLSESLEGLSAAMIAELGRYRHQVFIEKLGWDVVSTSRVRDQEFDQFDHPQTRYIVAMGRQGICGCARL
LPTTDAYLLKDVFAYLCSETPPSDPSVWELSRYAASAADDPQLAMKIFWSSLQCGWYLGASSVVAVTTTAME
RYFVRNGVILQRLGPPQKVKGETLVAISFPAYQERGLEMLLRYHPEWLQGVPLSMAV*
AnnotationID:3826GeneID:3819
AnnotatorUID: diggins
Modification Date/Time: 2005-07-31 11:11:47
Gene Name:rhlI
Confidence Code:1
GeneProduct:autoinducer synthesis protein RhlI
Cell Localization:()
Synonyms:vsmI
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00765,COG3916
Secondary Functional Category(ies):signal transduction mechanisms
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAF61720.1|AF232769_2 (AF232769) putative acylhomoserine lactone synthase [Pseudomonas
           fluorescens]
          Length = 219

 Score =  193 bits (490), Expect = 6e-49
 Identities = 95/181 (52%), Positives = 124/181 (68%), Gaps = 1/181 (0%)

>gb|AAK73191.1| (AY040629) N-acyl-homoserine lactone synthase CsaI [Pseudomonas
           chlororaphis]
          Length = 219

 Score =  189 bits (481), Expect = 7e-48
 Identities = 95/181 (52%), Positives = 124/181 (68%), Gaps = 1/181 (0%)

>gb|AAF17493.1|AF195615_1 (AF195615) autoinducer synthase PhzI [Pseudomonas chlororaphis]
          Length = 196

 Score =  138 bits (348), Expect = 2e-32
 Identities = 69/172 (40%), Positives = 104/172 (60%), Gaps = 4/172 (2%)







 
Structural Features:
pfam00765, Autoind_synth, Autoinducer synthetase.
CD-Length = 182 residues,  97.3% aligned
Score = 82.6 bits (204), Expect = 3e-17
Identities = 41/180 (22%), Positives = 77/180 (42%), Gaps = 11/180 (6%)

COG3916, LasI, N-acyl-L-homoserine lactone synthetase 
[Signal transduction mechanisms / 
Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 209 residues,  99.5% aligned
Score =  214 bits (547), Expect = 5e-57
Identities = 81/212 (38%), Positives = 107/212 (50%), Gaps = 15/212 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7641




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24783




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32083




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3819_cdd.html


    		




 
URL:

















    ReferenceID:34083




  
Author/Investigator(s):
        Chapon-Herve V, Akrim M, Latifi A, Williams P, Lazdunski A, Bally M.





  
Title:
        Regulation of the xcp secretion pathway by multiple quorum-sensing modulons in Pseudomonas aeruginosa.




  
PubMed:
        9218766


MedLine:
        




    
Source:
Mol Microbiol. 1997 Jun;24(6):1169-78.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9218766

















    ReferenceID:34084




  
Author/Investigator(s):
        Winson MK, Camara M, Latifi A, Foglino M, Chhabra SR, Daykin M, Bally M, Chapon V, Salmond GP, Bycroft BW, et al.





  
Title:
        Multiple N-acyl-L-homoserine lactone signal molecules regulate production of virulence determinants and secondary metabolites in Pseudomonas aeruginosa.




  
PubMed:
        7568146


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9427-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7568146

















    ReferenceID:34085




  
Author/Investigator(s):
        Parsek MR, Val DL, Hanzelka BL, Cronan JE Jr, Greenberg EP.





  
Title:
        Acyl homoserine-lactone quorum-sensing signal generation.




  
PubMed:
        10200267


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4360-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=10200267

















    ReferenceID:34086




  
Author/Investigator(s):
        Pesci EC, Pearson JP, Seed PC, Iglewski BH.





  
Title:
        Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa.




  
PubMed:
        9150205


MedLine:
        




    
Source:
J Bacteriol. 1997 May;179(10):3127-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9150205









           
Homologs By Global Alignment 

           

       



     
Gene ID:3819
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6040
                           PA3476_tr
                           translation of PA3476
                           201
                           99.00
                           99.00
                           0
                           1037.0
     

     
     
168236
                           gb|AAA82725.1|
                           (U11811) putative autoinducer synthetase [Pseudomonas aeruginosa]
                           201
                           99.00
                           99.50
                           0
                           1039.0
     

     
     
168237
                           gb|AAG06864.1|AE004768_9
                           (AE004768) autoinducer synthesis protein RhlI [Pseudomonas aeruginosa PAO1]
                           201
                           99.00
                           99.00
                           0
                           1037.0
     

     
     
168238
                           gb|AAA89074.1|
                           (U15644) VSMI [Pseudomonas aeruginosa]
                           201
                           97.01
                           97.51
                           1
                           1006.0
     

     
     
168239
                           gb|AAC44037.1|
                           (U40458) RhlI [Pseudomonas aeruginosa]
                           201
                           94.52
                           94.52
                           5
                           987.0
     

     






      

          
GC ORFID: 46821How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3476Glimmer Score: 

          
Start: 3302037Stop: 3301432

          
Length: 606


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3476 translation of PA3476Homolog Bit Score 404.0

          
Other Homologs:  t_PA3476 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCGAATTGCTCTCTGAATCGCTGGAAGGGCTTTCCGCCGCCATGATCGCCGAGCTGGGACGCTACCGG 
CATCAAGTCTTCATCGAGAAGCTGGGCTGGGACGTGGTCTCCACCTCCAGGGTCCGCGACCAGGAATTCGAC 
CAGTTCGACCATCCGCAAACCCGCTACATCGTCGCCATGGGCCGCCAGGGCATCTGCGGTTGCGCCCGCCTG 
CTGCCGACGACCGACGCCTACCTGCTCAAGGACGTCTTCGCCTACCTGTGCAGCGAAACCCCGCCGAGCGAT 
CCGTCGGTCTGGGAGCTTTCGCGCTACGCCGCCAGCGCGGCGGACGATCCGCAGCTGGCGATGAAGATATTC 
TGGTCCAGCCTGCAATGCGGCTGGTACCTGGGGGCCAGTTCGGTGGTGGCGGTGACCACCACGGCCATGGAG 
CGCTATTTCGTTCGCAACGGCGTGATCCTCCAGCGCCTCGGCCCGCCGCAGAAGGTCAAGGGCGAGACGCTG 
GTCGCGATCAGCTTCCCGGCCTACCAGGAGCGCGGCCTGGAGATGCTGTTGCGCTACCACCCGGAATGGCTG 
CAGGGTGTACCGCTGTCGATGGCGGTGTGA