ViewGene
Locus Name:
GeneID:
Location:

GeneID:3812
TrivialName: PA14_42250
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_42250
PAO1 Ortholog Locus:PA1725
Sequence Length:645
Protein Length:214
Start: 3769311
Stop: 3768667
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05114.1|AE004598_13 (AE004598) type III export protein PscL [Pseudomonas aeruginosa PAO1]
Identities = 212/214 (99%), Positives = 212/214 (99%)
Sequence:
ATGCTTCCATTTGTTGAACTAGACGCCAGTCGCGTACGGCTGGCCCCCGGGCAGGCCCTGTTGCGCGCCCGC
GACTACCAGGACTACCTCAGCGCCAACCGCCTGGTGGAAGCGGCACGCGAGCGTGCCGCGGAAATCGAGCGC
GAGGCCCACGAGGTTTACCAGGAGCAGAAGCGCCTGGGCTGGGAGGCCGGACTGGAGGAGGCGCGTCTGCGC
CAGGCCGGGCTGATCCAGGAAACGCTACTGCGCTGCAATCGCTACTACCGGCAGGTCGATCGCCAGCTTGGC
GAAGTGGTGCTGCAGGCGGTGCGCAAGGTGTTGCGCCACTACGACGCTGTCGAGCTGACCCTGGCGGCCACG
CGCGAGGCACTGGCATTGGTGAGCAACCAGAAGCAGGTGATCCTGCACGTCCAGCCGGAACAGCTGGTCGCG
GTGCGCGAGCAGGTCGCCCGGGTGCTCAAGGATTTCCCCGAGGTGGGCTACCTGGAAGTGGTCGGCGATGCC
CGGCTGGACCAGGGCGGTTGCATCCTGGAAACCGAGATCGGGATCATCGACGCATCGCTCGACAGCCAGTTG
GCGGCGTTGCAGGCCGCATTGACGGAGGGCGTGGCGCGGAGCGGCGAGGAGGAAGGGGACGCCGGTTGA
Translation:
MLPFVELDASRVRLAPGQALLRARDYQDYLSANRLVEAARERAAEIEREAHEVYQEQKRLGWEAGLEEARLR
QAGLIQETLLRCNRYYRQVDRQLGEVVLQAVRKVLRHYDAVELTLAATREALALVSNQKQVILHVQPEQLVA
VREQVARVLKDFPEVGYLEVVGDARLDQGGCILETEIGIIDASLDSQLAALQAALTEGVARSGEEEGDAG*
AnnotationID:3819GeneID:3812
AnnotatorUID: diggins
Modification Date/Time: 2005-02-28 07:07:32
Gene Name:pscL
Confidence Code:1
GeneProduct:type III export protein PscL
Cell Localization:()
Synonyms:yscL
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(19) Protein secretion/export apparatus
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1317
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO18079.1| (AY144116) LscL [Photorhabdus luminescens]
          Length = 205

 Score =  248 bits (634), Expect = 1e-65
 Identities = 122/199 (61%), Positives = 155/199 (77%)

>gb|AAN37554.1| (AY150843) YscL [Yersinia enterocolitica]
          Length = 223

 Score =  237 bits (604), Expect = 4e-62
 Identities = 121/212 (57%), Positives = 158/212 (74%), Gaps = 2/212 (0%)

>gb|AAC69773.1| (AF074612) type III secretion protein [Yersinia pestis KIM]
          Length = 221

 Score =  235 bits (599), Expect = 2e-61
 Identities = 116/200 (58%), Positives = 152/200 (76%)








 
Structural Features:
COG1317, FliH, Flagellar biosynthesis/type III secretory pathway protein 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 234 residues,  92.7% aligned
Score =  124 bits (312), Expect = 8e-30
Identities = 81/214 (37%), Positives = 110/214 (51%), Gaps = 13/214 (6%)







   
Genomic Context:






   
Comment:


















    ReferenceID:7627




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24769




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37207




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3812_cdd.html


    		




 
URL:

















    ReferenceID:37843




  
Author/Investigator(s):
        Yahr TL, Goranson J, Frank DW.





  
Title:
        Exoenzyme S of Pseudomonas aeruginosa is secreted by a type III pathway.




  
PubMed:
        8971719


MedLine:
        




    
Source:
Mol Microbiol. 1996 Dec;22(5):991-1003.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=97126825&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:3812
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6033
                           PA1725_tr
                           translation of PA1725
                           214
                           99.06
                           99.06
                           0
                           1042.0
     

     
     
168100
                           gb|AAG05114.1|AE004598_13
                           (AE004598) type III export protein PscL [Pseudomonas aeruginosa PAO1]
                           214
                           99.06
                           99.06
                           0
                           1042.0
     

     
     
168101
                           gb|AAC44783.1|
                           (U56077) PscL [Pseudomonas aeruginosa]
                           231
                           88.31
                           88.74
                           17
                           994.0
     

     
     
168102
                           emb|CAE16159.1|
                           (BX571871) Type III secretion component protein SctL [Photorhabdus luminescens subsp. laumondii TTO1]
                           214
                           57.94
                           72.89
                           9
                           636.0
     

     
     
168103
                           gb|AAO18079.1|
                           (AY144116) LscL [Photorhabdus luminescens]
                           214
                           57.00
                           72.42
                           9
                           628.0
     

     






      

          
GC ORFID: 46814How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1725Glimmer Score: 

          
Start: 1184598Stop: 1185242

          
Length: 645


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1725 translation of PA1725Homolog Bit Score 405.0

          
Other Homologs:  t_PA1725 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTTCCATTTGTTGAACTAGACGCCAGTCGCGTACGGCTGGCCCCCGGGCAGGCCCTGTTGCGCGCCCGC 
GACTACCAGGACTACCTCAGCGCCAACCGCCTGGTGGAAGCGGCACGCGAGCGTGCCGCGGAAATCGAGCGC 
GAGGCCCACGAGGTTTACCAGGAGCAGAAGCGCCTGGGCTGGGAGGCCGGACTGGAGGAGGCGCGTCTGCGC 
CAGGCCGGGCTGATCCAGGAAACGCTACTGCGCTGCAATCGCTACTACCGGCAGGTCGATCGCCAGCTTGGC 
GAAGTGGTGCTGCAGGCGGTGCGCAAGGTGTTGCGCCACTACGACGCTGTCGAGCTGACCCTGGCGGCCACG 
CGCGAGGCACTGGCATTGGTGAGCAACCAGAAGCAGGTGATCCTGCACGTCCAGCCGGAACAGCTGGTCGCG 
GTGCGCGAGCAGGTCGCCCGGGTGCTCAAGGATTTCCCCGAGGTGGGCTACCTGGAAGTGGTCGGCGATGCC 
CGGCTGGACCAGGGCGGTTGCATCCTGGAAACCGAGATCGGGATCATCGACGCATCGCTCGACAGCCAGTTG 
GCGGCGTTGCAGGCCGCATTGACGGAGGGCGTGGCGCGGAGCGGCGAGGAGGAAGGGGACGCCGGTTGA