ViewGene
Locus Name:
GeneID:
Location:

GeneID:3799
TrivialName: PA14_30310
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_30310
PAO1 Ortholog Locus:PA2614
Sequence Length:627
Protein Length:208
Start: 2625132
Stop: 2625758
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06002.1|AE004690_2 (AE004690) periplasmic chaperone LolA [Pseudomonas aeruginosa PAO1]
Identities = 208/208 (100%), Positives = 208/208 (100%)
Sequence:
ATGCGACTGATCCGCACGTTGTTCGTTGCCGCCCTGGCCATGGGCGCTTCGCTGGCCCATGCCGACGACAGC
GCCGCTGTCCAGCGCCTGACCGGCCTGCTGAATAAGGCCCAGACCCTGACCGCGCGTTTTTCCCAACTGACC
CTGGACGGTAGCGGCACGCGCCTGCAGGAAACCGCCGGCCAACTCAGCCTGAAGCGGCCGGGACTGTTCCGC
TGGCATACCGATGCGCCGAACGAGCAATTGCTCATTTCCAACGGCGAGAAGGTCTGGCTGTACGACCCGGAC
CTGGAGCAGGTGACCATCCAGAAGCTCGATCAGCGCCTGACCCAGACGCCGGCGCTGCTGCTCTCCGGCGAC
ATCTCGAAGATCAGCGAAAGCTTCGCCATCACCTACAAGGAAGGCGGCAACGTAGTCGATTTCGTACTCAAG
CCGAAGACCAAGGACACCCTCTTCGACACCTTGCGCCTGTCGTTCCGCAGCGGCAAGGTGAACGACATGCAG
ATGATCGATGGCGTCGGCCAGCGCACCAACATCCTGTTCTTCGACGTGAAGATGAACGAGGCGCTGGATGCC
AAGCAATTCACCTTCGACGTTCCGCCGGGCGTCGACGTGATCCAGGAGTAA
Translation:
MRLIRTLFVAALAMGASLAHADDSAAVQRLTGLLNKAQTLTARFSQLTLDGSGTRLQETAGQLSLKRPGLFR
WHTDAPNEQLLISNGEKVWLYDPDLEQVTIQKLDQRLTQTPALLLSGDISKISESFAITYKEGGNVVDFVLK
PKTKDTLFDTLRLSFRSGKVNDMQMIDGVGQRTNILFFDVKMNEALDAKQFTFDVPPGVDVIQE*
AnnotationID:3806GeneID:3799
AnnotatorUID: diggins
Modification Date/Time: 2005-03-07 11:11:48
Gene Name:lolA
Confidence Code:2
GeneProduct:periplasmic chaperone protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:outer membrane lipoprotein carrier protein
Functional Category Confidence Code:5
COGs:pfam03548,COG2834
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAC98299.1| (AF095845) periplasmic chaperone protein [Pseudomonas syringae]
           [Pseudomonas syringae pv. syringae]
          Length = 207

 Score =  291 bits (745), Expect = 2e-78
 Identities = 149/208 (71%), Positives = 171/208 (82%), Gaps = 1/208 (0%)

>gb|AAN69597.1|AE016593_5 (AE016788) outer membrane lipoprotein carrier protein [Pseudomonas
           putida KT2440]
          Length = 228

 Score =  265 bits (676), Expect = 2e-70
 Identities = 134/208 (64%), Positives = 165/208 (79%), Gaps = 1/208 (0%)

>gb|AAO90699.1| (AE016963) outer membrane lipoprotein carrier protein LolA
           [Coxiella burnetii RSA 493]
          Length = 211

 Score =  131 bits (329), Expect = 3e-30
 Identities = 65/186 (34%), Positives = 110/186 (59%), Gaps = 1/186 (0%)








 
Structural Features:
pfam03548, LolA, Outer membrane lipoprotein carrier protein LolA.
CD-Length = 199 residues, 100.0% aligned
Score =  263 bits (673), Expect = 1e-71
Identities = 104/199 (52%), Positives = 134/199 (67%)

COG2834, LolA, Outer membrane lipoprotein-sorting protein 
[Cell envelope biogenesis, outer membrane]
CD-Length = 211 residues,  98.1% aligned
Score =  168 bits (426), Expect = 5e-43
Identities = 69/209 (33%), Positives = 103/209 (49%), Gaps = 4/209 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7601




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24743




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33178




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3799_cdd.html


    		




 
URL:

















    ReferenceID:34547




  
Author/Investigator(s):
        Yakushi T, Masuda K, Narita S, Matsuyama S, Tokuda H.





  
Title:
        A new ABC transporter mediating the detachment of lipid-modified proteins from membranes.




  
PubMed:
        10783239


MedLine:
        




    
Source:
Nat Cell Biol. 2000 Apr;2(4):212-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10783239

















    ReferenceID:34548




  
Author/Investigator(s):
        Yokota N, Kuroda T, Matsuyama S, Tokuda H.





  
Title:
        Characterization of the LolA-LolB system as the general lipoprotein localization mechanism of Escherichia coli.




  
PubMed:
        10521496


MedLine:
        




    
Source:
J Biol Chem. 1999 Oct 22;274(43):30995-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10521496

















    ReferenceID:34549




  
Author/Investigator(s):
        Tajima T, Yokota N, Matsuyama S, Tokuda H.





  
Title:
        Genetic analyses of the in vivo function of LolA, a periplasmic chaperone involved in the outer membrane localization of Escherichia coli lipoproteins.




  
PubMed:
        9849875


MedLine:
        




    
Source:
FEBS Lett. 1998 Nov 13;439(1-2):51-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:ttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9849875









           
Homologs By Global Alignment 

           

       



     
Gene ID:3799
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
6020
                           PA2614_tr
                           translation of PA2614
                           208
                           99.99
                           99.99
                           0
                           1046.0
     

     
     
167842
                           gb|AAG06002.1|AE004690_2
                           (AE004690) periplasmic chaperone LolA [Pseudomonas aeruginosa PAO1]
                           208
                           99.99
                           99.99
                           0
                           1046.0
     

     
     
167843
                           gb|AAC98299.1|
                           (AF095845) periplasmic chaperone protein [Pseudomonas syringae] [Pseudomonas syringae pv. syringae]
                           208
                           71.63
                           82.21
                           1
                           747.0
     

     
     
167844
                           gb|AAO56826.1|
                           (AE016868) outer membrane lipoprotein carrier protein [Pseudomonas syringae pv. tomato str. DC3000]
                           208
                           71.15
                           82.21
                           1
                           745.0
     

     
     
167845
                           gb|AAN69597.1|AE016593_5
                           (AE016788) outer membrane lipoprotein carrier protein [Pseudomonas putida KT2440]
                           229
                           58.51
                           72.05
                           22
                           678.0
     

     






      

          
GC ORFID: 46801How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2614Glimmer Score: 

          
Start: 2328794Stop: 2328168

          
Length: 627


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2614 translation of PA2614Homolog Bit Score 407.0

          
Other Homologs:  psyr_15may02_Scaffold17_revised_gene2813 (ORF: BLASTX 291.0), t_PA2614 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGACTGATCCGCACGTTGTTCGTTGCCGCCCTGGCCATGGGCGCTTCGCTGGCCCATGCCGACGACAGC 
GCCGCTGTCCAGCGCCTGACCGGCCTGCTGAATAAGGCCCAGACCCTGACCGCGCGTTTTTCCCAACTGACC 
CTGGACGGTAGCGGCACGCGCCTGCAGGAAACCGCCGGCCAACTCAGCCTGAAGCGGCCGGGACTGTTCCGC 
TGGCATACCGATGCGCCGAACGAGCAATTGCTCATTTCCAACGGCGAGAAGGTCTGGCTGTACGACCCGGAC 
CTGGAGCAGGTGACCATCCAGAAGCTCGATCAGCGCCTGACCCAGACGCCGGCGCTGCTGCTCTCCGGCGAC 
ATCTCGAAGATCAGCGAAAGCTTCGCCATCACCTACAAGGAAGGCGGCAACGTAGTCGATTTCGTACTCAAG 
CCGAAGACCAAGGACACCCTCTTCGACACCTTGCGCCTGTCGTTCCGCAGCGGCAAGGTGAACGACATGCAG 
ATGATCGATGGCGTCGGCCAGCGCACCAACATCCTGTTCTTCGACGTGAAGATGAACGAGGCGCTGGATGCC 
AAGCAATTCACCTTCGACGTTCCGCCGGGCGTCGACGTGATCCAGGAGTAA