ViewGene
Locus Name:
GeneID:
Location:

GeneID:3751
TrivialName: PA14_01910
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_01910
PAO1 Ortholog Locus:PA0154
Sequence Length:606
Protein Length:201
Start: 174354
Stop: 174959
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03544.1|AE004453_7 (AE004453) protocatechuate 3,4-dioxygenase, alpha subunit [Pseudomonas aeruginosa PAO1]
Identities = 199/201 (99%), Positives = 200/201 (99%)
Sequence:
ATGAGCCACGAACTGCTGCGGGAAACCCCTTCCCAGACCGCCGGTCCCTACGTCCACATCGGCCTGGCGCTG
GCCGCCGCCGGCAACCCGACCCGCGACCTGGAGATCTGGAACCGCATGGCCCGCGAAGGCGCGCCGGGCCAG
CACATCCTCCTGCTCGGCCACGTCTACGACGGCAACGGCCACCTGGTGCGCGACTCGTTCCTGGAGTTCTGG
CAGGCCGACAGCGAAGGCCGCTACCAGGAGCGCTACGATCCGGAGCGACCGTTCAACAGCTTCGGCCGCACC
GCCACCACCTTCGATGCCGGCGAGTGGACCCTGCACACGGTCAAGCCGGGGGTGGTGCGTAACGCCCAGGGC
GTGCCGATGGCGCCGCACATCAACGTCTCGCTGTTCGCCCGCGGCATCAACATCCACTTGCAGACCCGGCTG
TACTTCGATGACGAGACCGAGGCCAACGCCGCCTGTCCGGTGCTCAACCTGATCGAGCAGGCGCCGCGTCGC
GACACCCTGCTCGCGCGGCGCTGCGAAGTGGACGGACAGCTCGCCTACCGTTTCGACATCCGCATCCAGGGC
GACAACGAGACGGTGTTCTTCGACTTCTAG
Translation:
MSHELLRETPSQTAGPYVHIGLALAAAGNPTRDLEIWNRMAREGAPGQHILLLGHVYDGNGHLVRDSFLEFW
QADSEGRYQERYDPERPFNSFGRTATTFDAGEWTLHTVKPGVVRNAQGVPMAPHINVSLFARGINIHLQTRL
YFDDETEANAACPVLNLIEQAPRRDTLLARRCEVDGQLAYRFDIRIQGDNETVFFDF*
AnnotationID:3758GeneID:3751
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 06:06:56
Gene Name:pcaG
Confidence Code:2
GeneProduct:protocatechuate 3,4-dioxygenase, alpha subunit
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3485
Secondary Functional Category(ies):biosynthesis of cofactors, prosthetic groups and carriers
EC Number:1.13.11.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAG17459.1|AF302797_5 (AF302797) protocatechuate 3,4 dioxygenase alpha subunit
           [Pseudomonas sp. IMT40] [Pseudomonas indica]
          Length = 201

 Score =  346 bits (888), Expect = 4e-95
 Identities = 165/201 (82%), Positives = 179/201 (89%)

>gb|AAB41025.1| (L14836) protocatechuate 3,4-dioxygenase, alpha subunit
           [Pseudomonas putida]
          Length = 201

 Score =  344 bits (883), Expect = 2e-94
 Identities = 164/201 (81%), Positives = 177/201 (88%)








 
Structural Features:
COG3485, PcaH, Protocatechuate 3,4-dioxygenase beta subunit 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 226 residues,  90.3% aligned
Score =  187 bits (477), Expect = 6e-49
Identities = 96/208 (46%), Positives = 117/208 (56%), Gaps = 12/208 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7505




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24647




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33526




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3751_cdd.html


    		




 
URL:

















    ReferenceID:35491




  
Author/Investigator(s):
        Frazee RW, Livingston DM, LaPorte DC, Lipscomb JD.





  
Title:
        Cloning, sequencing, and expression of the Pseudomonas putida protocatechuate 3,4-dioxygenase genes.




  
PubMed:
        8407791


MedLine:
        




    
Source:
J Bacteriol. 1993 Oct;175(19):6194-202.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8407791

















    ReferenceID:35492




  
Author/Investigator(s):
        Petersen E, Zuegg J, Ribbons DW, Schwab H.





  
Title:
        Molecular cloning and homology modeling of protocatechuate 3,4-dioxygenase from Pseudomonas marginata.




  
PubMed:
        9022300


MedLine:
        




    
Source:
Microbiol Res. 1996 Dec;151(4):359-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9022300









           
Homologs By Global Alignment 

           

       



     
Gene ID:3751
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5965
                           PA0154_tr
                           translation of PA0154
                           201
                           99.00
                           99.50
                           0
                           1062.0
     

     
     
166535
                           gb|AAG03544.1|AE004453_7
                           (AE004453) protocatechuate 3,4-dioxygenase, alpha subunit [Pseudomonas aeruginosa PAO1]
                           201
                           99.00
                           99.50
                           0
                           1062.0
     

     
     
166536
                           gb|AAG17459.1|AF302797_5
                           (AF302797) protocatechuate 3,4 dioxygenase alpha subunit [Pseudomonas sp. IMT40] [Pseudomonas indica]
                           201
                           82.08
                           89.05
                           0
                           888.0
     

     
     
166537
                           gb|AAB41025.1|
                           (L14836) protocatechuate 3,4-dioxygenase, alpha subunit [Pseudomonas putida]
                           201
                           81.59
                           88.05
                           0
                           883.0
     

     
     
166538
                           gb|AAN70228.1|AE016662_4
                           (AE016791) protocatechuate 3,4-dioxygenase, alpha subunit [Pseudomonas putida KT2440]
                           201
                           80.59
                           87.56
                           0
                           873.0
     

     






      

          
GC ORFID: 46753How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0154Glimmer Score: 

          
Start: 5975693Stop: 5976298

          
Length: 606


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA0154 translation of PA0154Homolog Bit Score 413.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1095 (ORF: BLASTX 313.0), t_PA0154 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCACGAACTGCTGCGGGAAACCCCTTCCCAGACCGCCGGTCCCTACGTCCACATCGGCCTGGCGCTG 
GCCGCCGCCGGCAACCCGACCCGCGACCTGGAGATCTGGAACCGCATGGCCCGCGAAGGCGCGCCGGGCCAG 
CACATCCTCCTGCTCGGCCACGTCTACGACGGCAACGGCCACCTGGTGCGCGACTCGTTCCTGGAGTTCTGG 
CAGGCCGACAGCGAAGGCCGCTACCAGGAGCGCTACGATCCGGAGCGACCGTTCAACAGCTTCGGCCGCACC 
GCCACCACCTTCGATGCCGGCGAGTGGACCCTGCACACGGTCAAGCCGGGGGTGGTGCGTAACGCCCAGGGC 
GTGCCGATGGCGCCGCACATCAACGTCTCGCTGTTCGCCCGCGGCATCAACATCCACTTGCAGACCCGGCTG 
TACTTCGATGACGAGACCGAGGCCAACGCCGCCTGTCCGGTGCTCAACCTGATCGAGCAGGCGCCGCGTCGC 
GACACCCTGCTCGCGCGGCGCTGCGAAGTGGACGGACAGCTCGCCTACCGTTTCGACATCCGCATCCAGGGC 
GACAACGAGACGGTGTTCTTCGACTTCTAG