ViewGene
Locus Name:
GeneID:
Location:

GeneID:3745
TrivialName: PA14_62870
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62870
PAO1 Ortholog Locus:PA4752
Sequence Length:624
Protein Length:207
Start: 5610452
Stop: 5609829
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08138.1|AE004889_1 (AE004889) cell division protein FtsJ [Pseudomonas aeruginosa PAO1]
Identities = 207/207 (100%), Positives = 207/207 (100%)
Sequence:
GTGGCCCGCTCCAAAACCAGCCAGCGTTGGCTGAAAGAACATTTCGACGATCCCTACGTGAAGATGGCCCAG
CGCGATGGCTATCGCTCGCGCGCCAGCTACAAGCTGCTGGAGATCCAGGAGAAGGACCGCATCCTGCGTCCG
GGCATGACCGTGGTCGACCTGGGCGCCGCGCCGGGCGGGTGGTCGCAGGTCACCAGCCGGGTGATCGGCGAC
CGTGGTCGGCTGATCGCTTCGGACATCCTCGAGATGGACAGCATCCCCGACGTCACCTTCATCCAGGGCGAC
TTCACCGAGGACGCGGTGTTCGCGCGGATCCTCGAGGCGATCGGCGATCATCCGGTAGACCTTGTGATTTCC
GATATGGCCCCCAATATGAGTGGAGTGCGGGCCGCCGACCAGCCGCGCGCCATGTATCTCTGCGAACTGGCG
CTGGACCTGGCCGGCCGTGTGCTGCGGCCGGGCGGCGATTTCCTGATCAAGATCTTCCAGGGCGAAGGTTTC
GACCAGTACCACAAGCAGGCCCGGGAAATGTTCGACAAGGTACAGATGCGCAAGCCCCTGTCGTCGCGTGAT
CGTTCGCGGGAACAGTATCTGCTCGCACGCGGCTTTCGCGGGGAATAG
Translation:
MARSKTSQRWLKEHFDDPYVKMAQRDGYRSRASYKLLEIQEKDRILRPGMTVVDLGAAPGGWSQVTSRVIGD
RGRLIASDILEMDSIPDVTFIQGDFTEDAVFARILEAIGDHPVDLVISDMAPNMSGVRAADQPRAMYLCELA
LDLAGRVLRPGGDFLIKIFQGEGFDQYHKQAREMFDKVQMRKPLSSRDRSREQYLLARGFRGE*
AnnotationID:3752GeneID:3745
AnnotatorUID: diggins
Modification Date/Time: 2005-05-24 08:08:00
Gene Name:rrmJ
Confidence Code:2
GeneProduct:cell division protein FtsJ
Cell Localization:()
Synonyms:ftsJ
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(6) Cell division
Alternate Gene Product Name:ribosomal RNA large subunit methyltransferase J
Functional Category Confidence Code:5
COGs:COG0293,pfam01728
Secondary Functional Category(ies):translation, ribosomal structure and biogenesis
EC Number:2.1.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57946.1| (AE016872) FtsJ cell division protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 216

 Score =  360 bits (925), Expect = 2e-99
 Identities = 176/206 (85%), Positives = 191/206 (92%)

>gb|AAN70291.1|AE016670_1 (AE016791) ribosomal RNA large subunit methyltransferase J
           [Pseudomonas putida KT2440]
          Length = 207

 Score =  334 bits (856), Expect = 2e-91
 Identities = 165/204 (80%), Positives = 180/204 (88%)

>gb|AAN82376.1|AE016767_136 (AE016767) Ribosomal RNA large subunit methyltransferase J
           [Escherichia coli CFT073]
          Length = 209

 Score =  244 bits (624), Expect = 2e-64
 Identities = 119/201 (59%), Positives = 151/201 (75%)






 
Structural Features:
COG0293, FtsJ, 23S rRNA methylase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 205 residues,  99.0% aligned
Score =  283 bits (726), Expect = 9e-78
Identities = 117/203 (57%), Positives = 154/203 (75%)

pfam01728, FtsJ, FtsJ-like methyltransferase. 
CD-Length = 183 residues, 100.0% aligned
Score =  183 bits (467), Expect = 9e-48
Identities = 83/183 (45%), Positives = 121/183 (66%), Gaps = 5/183 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14911




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24635




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37669




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3745_cdd.html


    		




 
URL:

















    ReferenceID:37720




  
Author/Investigator(s):
        Caldas T, Binet E, Bouloc P, Costa A, Desgres J, Richarme G.





  
Title:
        The FtsJ/RrmJ heat shock protein of Escherichia coli is a 23 S ribosomal RNA methyltransferase.




  
PubMed:
        10748051


MedLine:
        




    
Source:
 J Biol Chem. 2000 Jun 2;275(22):16414-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10748051









           
Homologs By Global Alignment 

           

       



     
Gene ID:3745
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5958
                           PA4752_tr
                           translation of PA4752
                           207
                           99.99
                           99.99
                           0
                           1064.0
     

     
     
166444
                           gb|AAG08138.1|AE004889_1
                           (AE004889) cell division protein FtsJ [Pseudomonas aeruginosa PAO1]
                           207
                           99.51
                           99.99
                           0
                           1061.0
     

     
     
166445
                           gb|AAO57946.1|
                           (AE016872) FtsJ cell division protein [Pseudomonas syringae pv. tomato str. DC3000]
                           216
                           81.01
                           88.42
                           9
                           918.0
     

     
     
166446
                           gb|AAN70291.1|AE016670_1
                           (AE016791) ribosomal RNA large subunit methyltransferase J [Pseudomonas putida KT2440]
                           208
                           79.80
                           87.01
                           2
                           859.0
     

     
     
166447
                           gb|AAO10105.1|AE016802_148
                           (AE016802) 23S rRNA methylase [Vibrio vulnificus CMCP6]
                           210
                           60.47
                           75.23
                           4
                           661.0
     

     






      

          
GC ORFID: 46747How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4752Glimmer Score: 

          
Start: 4874132Stop: 4873509

          
Length: 624


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4752 translation of PA4752Homolog Bit Score 414.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5484 (ORF: BLASTX 360.0), t_PA4752 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGGCCCGCTCCAAAACCAGCCAGCGTTGGCTGAAAGAACATTTCGACGATCCCTACGTGAAGATGGCCCAG 
CGCGATGGCTATCGCTCGCGCGCCAGCTACAAGCTGCTGGAGATCCAGGAGAAGGACCGCATCCTGCGTCCG 
GGCATGACCGTGGTCGACCTGGGCGCCGCGCCGGGCGGGTGGTCGCAGGTCACCAGCCGGGTGATCGGCGAC 
CGTGGTCGGCTGATCGCTTCGGACATCCTCGAGATGGACAGCATCCCCGACGTCACCTTCATCCAGGGCGAC 
TTCACCGAGGACGCGGTGTTCGCGCGGATCCTCGAGGCGATCGGCGATCATCCGGTAGACCTTGTGATTTCC 
GATATGGCCCCCAATATGAGTGGAGTGCGGGCCGCCGACCAGCCGCGCGCCATGTATCTCTGCGAACTGGCG 
CTGGACCTGGCCGGCCGTGTGCTGCGGCCGGGCGGCGATTTCCTGATCAAGATCTTCCAGGGCGAAGGTTTC 
GACCAGTACCACAAGCAGGCCCGGGAAATGTTCGACAAGGTACAGATGCGCAAGCCCCTGTCGTCGCGTGAT 
CGTTCGCGGGAACAGTATCTGCTCGCACGCGGCTTTCGCGGGGAATAG