ViewGene
Locus Name:
GeneID:
Location:

GeneID:3743
TrivialName: PA14_17460
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17460
PAO1 Ortholog Locus:PA3624
Sequence Length:636
Protein Length:211
Start: 1497418
Stop: 1498053
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07012.1|AE004782_10 (AE004782) L-isoaspartate protein carboxylmethyltransferase type II [Pseudomonas aeruginosa PAO1]
Identities = 211/211 (100%), Positives = 211/211 (100%)
Sequence:
ATGACCTCCCAGCGTACCCGAGAGCGCCTGATCCAGCGCCTGTACGAAGAAGGTCTGTCCAACGCCCACGTG
CTCGAGGTGATCCGCCGCACGCCGCGTCATCTGTTCGTCGACGAGGCGCTCTCGCATCGCGCCTACGAAGAC
ACTGCCCTGCCGATCGGCCACAACCAGACCATTTCCCAGCCGTTCATGGTGGCGCGGATGACCGAGTTGCTG
CTGGCGGCCGGCCCGCTGGACAAGGTCATGGAGATCGGTACCGGGTCCGGCTACCAGACCGCGGTGCTGGCC
CAACTGGTCGAGCGGGTCTTCTCCGTCGAGCGGATCCAGGCGTTGCAGGACAAGGCCAAGGAGCGTCTTGCG
GAGCTAAACCTGCGTAATGTTGTCTTTCGTTGGGGCGATGGCTGGGAGGGCTGGTCGGCGTTGGCTCCCTAC
AATGGAATCATCGTCACCGCGGCGGCCACGGAAGTCCCGCAGTCGTTGCTGGACCAGTTGGCGCCGGGGGGC
CGTCTGGTGATCCCGGTCGGTGGCGGCGAGGTCCAGCAACTGATGCTGATCGTCCGCACCGAGGACGGGTTC
AGCCGCCAGGTACTCGACTCGGTACGTTTCGTCCCGCTGCTCAACGGCCCGATCGCCTGA
Translation:
MTSQRTRERLIQRLYEEGLSNAHVLEVIRRTPRHLFVDEALSHRAYEDTALPIGHNQTISQPFMVARMTELL
LAAGPLDKVMEIGTGSGYQTAVLAQLVERVFSVERIQALQDKAKERLAELNLRNVVFRWGDGWEGWSALAPY
NGIIVTAAATEVPQSLLDQLAPGGRLVIPVGGGEVQQLMLIVRTEDGFSRQVLDSVRFVPLLNGPIA*
AnnotationID:3750GeneID:3743
AnnotatorUID: diggins
Modification Date/Time: 2005-06-20 21:09:13
Gene Name:pcm
Confidence Code:2
GeneProduct:L-isoaspartate protein carboxylmethyltransferase type II
Cell Localization:(1) Cytoplasmic
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:protein-L-isoaspartate O-methyltransferase
Functional Category Confidence Code:5
COGs:COG2518,pfam01135,KOG1661
Secondary Functional Category(ies):
EC Number:2.1.1.77
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55083.1| (AE016861) protein-L-isoaspartate O-methyltransferase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 211

 Score =  372 bits (954), Expect = e-102
 Identities = 185/211 (87%), Positives = 200/211 (94%)

>gb|AAN56431.1|AE015780_2 (AE015780) protein-L-isoaspartate O-methyltransferase [Shewanella
           oneidensis MR-1]
          Length = 211

 Score =  237 bits (605), Expect = 3e-62
 Identities = 120/202 (59%), Positives = 159/202 (78%), Gaps = 2/202 (0%)






 
Structural Features:
COG2518, Pcm, Protein-L-isoaspartate carboxylmethyltransferase 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 209 residues,  97.6% aligned
Score =  257 bits (658), Expect = 2e-69
Identities = 115/205 (56%), Positives = 140/205 (68%), Gaps = 2/205 (0%)

pfam01135, PCMT, Protein-L-isoaspartate(D-aspartate) 
O-methyltransferase (PCMT).
CD-Length = 210 residues,  99.0% aligned
Score =  204 bits (520), Expect = 2e-53
Identities = 92/209 (44%), Positives = 119/209 (56%), Gaps = 6/209 (2%)

KOG1661, Protein-L-isoaspartate(D-aspartate) 
O-methyltransferase
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 237 residues,  93.2% aligned
Score =  111 bits (279), Expect = 1e-25
Identities = 82/221 (37%), Positives = 113/221 (51%), Gaps = 18/221 (8%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7489




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24631




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31918




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3743_cdd.html


    		




 
URL:

















    ReferenceID:34180




  
Author/Investigator(s):
        Fu JC, Ding L, Clarke S.





  
Title:
        Purification, gene cloning, and sequence analysis of an L-isoaspartyl protein carboxyl methyltransferase from Escherichia coli.




  
PubMed:
        1860862


MedLine:
        




    
Source:
J Biol Chem. 1991 Aug 5;266(22):14562-72.

Erratum in:

    * J Biol Chem 1992 Jun 5;267(16):11660.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1860862









           
Homologs By Global Alignment 

           

       



     
Gene ID:3743
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5956
                           PA3624_tr
                           translation of PA3624
                           211
                           99.99
                           99.99
                           0
                           1063.0
     

     
     
166379
                           gb|AAG07012.1|AE004782_10
                           (AE004782) L-isoaspartate protein carboxylmethyltransferase type II [Pseudomonas aeruginosa PAO1]
                           211
                           99.99
                           99.99
                           0
                           1063.0
     

     
     
166380
                           gb|AAO55083.1|
                           (AE016861) protein-L-isoaspartate O-methyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           211
                           87.67
                           94.78
                           0
                           954.0
     

     
     
166381
                           gb|AAN67242.1|AE016351_4
                           (AE016780) protein-L-isoaspartate O-methyltransferase [Pseudomonas putida KT2440]
                           231
                           78.78
                           84.41
                           20
                           917.0
     

     
     
166382
                           gb|AAN56431.1|AE015780_2
                           (AE015780) protein-L-isoaspartate O-methyltransferase [Shewanella oneidensis MR-1]
                           216
                           56.48
                           75.00
                           10
                           606.0
     

     






      

          
GC ORFID: 46745How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3624Glimmer Score: 

          
Start: 3456519Stop: 3455884

          
Length: 636


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3624 translation of PA3624Homolog Bit Score 414.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4649 (ORF: BLASTX 387.0), t_PA3624 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCTCCCAGCGTACCCGAGAGCGCCTGATCCAGCGCCTGTACGAAGAAGGTCTGTCCAACGCCCACGTG 
CTCGAGGTGATCCGCCGCACGCCGCGTCATCTGTTCGTCGACGAGGCGCTCTCGCATCGCGCCTACGAAGAC 
ACTGCCCTGCCGATCGGCCACAACCAGACCATTTCCCAGCCGTTCATGGTGGCGCGGATGACCGAGTTGCTG 
CTGGCGGCCGGCCCGCTGGACAAGGTCATGGAGATCGGTACCGGGTCCGGCTACCAGACCGCGGTGCTGGCC 
CAACTGGTCGAGCGGGTCTTCTCCGTCGAGCGGATCCAGGCGTTGCAGGACAAGGCCAAGGAGCGTCTTGCG 
GAGCTAAACCTGCGTAATGTTGTCTTTCGTTGGGGCGATGGCTGGGAGGGCTGGTCGGCGTTGGCTCCCTAC 
AATGGAATCATCGTCACCGCGGCGGCCACGGAAGTCCCGCAGTCGTTGCTGGACCAGTTGGCGCCGGGGGGC 
CGTCTGGTGATCCCGGTCGGTGGCGGCGAGGTCCAGCAACTGATGCTGATCGTCCGCACCGAGGACGGGTTC 
AGCCGCCAGGTACTCGACTCGGTACGTTTCGTCCCGCTGCTCAACGGCCCGATCGCCTGA