ViewGene
Locus Name:
GeneID:
Location:

GeneID:3738
TrivialName: PA14_57530
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57530
PAO1 Ortholog Locus:PA4428
Sequence Length:618
Protein Length:205
Start: 5125745
Stop: 5125128
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07816.1|AE004857_7 (AE004857) stringent starvation protein A [Pseudomonas aeruginosa PAO1]
Identities = 205/205 (100%), Positives = 205/205 (100%)
Sequence:
ATGGCTTCAATCAACAAGCTGACCTGCTATTCCGACCCTGCCGATCATTACTGCCATCGCATCCGCCTGGTC
CTGGCCGAGAAGAACGTGGCGGTAGAGGTGATCGATATCGAGGCGGGCCGCTGTCCGCCGATGCTGGCGGAG
GTCAACCCCTACGGCAGCGTACCGACCCTGGTCGATCGCGACCTGGCGCTGTATGAGCCGACGGTGGTCATG
GAATACCTCGACGAACGCTATCCGCATCCGCCGCTGCTGCCCGATTATCCGGTGAAGCGTGCCAACAGCCGC
CTGTTGATCCATCGTATCCAGCGGGACTGGTGTTCCCTGGTGGACCGGATCCTGGATGCGCGCAGCAAGGAG
GCCGAGCGGGTCCAGGCGCGCAAGGAGTTGCGCGAGAGCCTGACCGGGGTTTCCCCGCTGTTCGCCGACAAG
GCATATTTCCTCAGCGAGGATTTCAGCCTGGTGGATTGCTGCCTGTTGCCGATCCTCTGGCGTCTGCCGCTG
CTGGGCATCGAGTTGCCGCGGCAGGCCAAGCCGTTGCTCGACTACATGGAGCGCGGATTCGCCCGGGAAAGC
TTCCGGGCCAGCCTGTCTTCCGTTGAACGAGATATGCGTTAA
Translation:
MASINKLTCYSDPADHYCHRIRLVLAEKNVAVEVIDIEAGRCPPMLAEVNPYGSVPTLVDRDLALYEPTVVM
EYLDERYPHPPLLPDYPVKRANSRLLIHRIQRDWCSLVDRILDARSKEAERVQARKELRESLTGVSPLFADK
AYFLSEDFSLVDCCLLPILWRLPLLGIELPRQAKPLLDYMERGFARESFRASLSSVERDMR*
AnnotationID:3745GeneID:3738
AnnotatorUID: diggins
Modification Date/Time: 2005-06-14 09:09:38
Gene Name:sspA
Confidence Code:2
GeneProduct:stringent starvation protein A
Cell Localization:(1) Cytoplasmic
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(1) Adaptation, protection
Alternate Gene Product Name:Glutathione S-transferase
Functional Category Confidence Code:5
COGs:COG0625
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57873.1| (AE016871) stringent starvation protein A [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 205

 Score =  343 bits (879), Expect = 5e-94
 Identities = 165/205 (80%), Positives = 182/205 (88%)

>gb|AAN66944.1|AE016322_11 (AE016779) stringent starvation protein A [Pseudomonas putida
           KT2440]
          Length = 207

 Score =  325 bits (833), Expect = 1e-88
 Identities = 156/205 (76%), Positives = 178/205 (86%)






 
Structural Features:
COG0625, Gst, Glutathione S-transferase 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 211 residues,  94.8% aligned
Score =  112 bits (282), Expect = 4e-26
Identities = 73/201 (36%), Positives = 98/201 (48%), Gaps = 13/201 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7479




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24621




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31468




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3738_cdd.html


    		




 
URL:

















    ReferenceID:32811




  
Author/Investigator(s):
        Williams MD, Ouyang TX, Flickinger MC.





  
Title:
        Starvation-induced expression of SspA and SspB: the effects of a null mutation in sspA on Escherichia coli protein synthesis and survival during growth and prolonged starvation.




  
PubMed:
        8022275


MedLine:
        




    
Source:
Mol Microbiol. 1994 Mar;11(6):1029-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8022275

















    ReferenceID:32812




  
Author/Investigator(s):
        Andrykovitch M, Routzahn KM, Li M, Gu Y, Waugh DS, Ji X.





  
Title:
        Characterization of four orthologs of stringent starvation protein A.




  
PubMed:
        12777805


MedLine:
        




    
Source:
Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):881-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12777805

















    ReferenceID:32813




  
Author/Investigator(s):
        Oishi K, Sar B, Wada A, Hidaka Y, Matsumoto S, Amano H, Sonoda F, Kobayashi S, Hirayama T, Nagatake T, Matsushima K.





  
Title:
        Nitrite reductase from Pseudomonas aeruginosa induces inflammatory cytokines in cultured respiratory cells.




  
PubMed:
        9199432


MedLine:
        




    
Source:
Infect Immun. 1997 Jul;65(7):2648-55.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9199432

















    ReferenceID:32814




  
Author/Investigator(s):
        Shankar S, Schlictman D, Chakrabarty AM.





  
Title:
        Regulation of nucleoside diphosphate kinase and an alternative kinase in Escherichia coli: role of the sspA and rnk genes in nucleoside triphosphate formation.




  
PubMed:
        8596442


MedLine:
        




    
Source:
Mol Microbiol. 1995 Sep;17(5):935-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8596442

















    ReferenceID:32815




  
Author/Investigator(s):
        Schlictman D, Shankar S, Chakrabarty AM.





  
Title:
        The Escherichia coli genes sspA and rnk can functionally replace the Pseudomonas aeruginosa alginate regulatory gene algR2.




  
PubMed:
        7565093


MedLine:
        




    
Source:
Mol Microbiol. 1995 Apr;16(2):309-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7565093









           
Homologs By Global Alignment 

           

       



     
Gene ID:3738
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5951
                           PA4428_tr
                           translation of PA4428
                           205
                           99.99
                           99.99
                           0
                           1069.0
     

     
     
166314
                           gb|AAG07816.1|AE004857_7
                           (AE004857) stringent starvation protein A [Pseudomonas aeruginosa PAO1]
                           205
                           99.99
                           99.99
                           0
                           1069.0
     

     
     
166315
                           gb|AAO57873.1|
                           (AE016871) stringent starvation protein A [Pseudomonas syringae pv. tomato str. DC3000]
                           205
                           80.48
                           88.78
                           0
                           879.0
     

     
     
166316
                           gb|AAN66944.1|AE016322_11
                           (AE016779) stringent starvation protein A [Pseudomonas putida KT2440]
                           207
                           75.36
                           85.99
                           2
                           833.0
     

     
     
166319
                           gb|AAN53689.1|AE015508_5
                           (AE015508) stringent starvation protein a [Shewanella oneidensis MR-1]
                           214
                           53.73
                           69.62
                           14
                           577.0
     

     






      

          
GC ORFID: 46740How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4428Glimmer Score: 

          
Start: 4389424Stop: 4388807

          
Length: 618


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4428 translation of PA4428Homolog Bit Score 416.0

          
Other Homologs:  psyr_15may02_Scaffold12_revised_gene2033 (ORF: BLASTX 339.0), t_PA4428 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTTCAATCAACAAGCTGACCTGCTATTCCGACCCTGCCGATCATTACTGCCATCGCATCCGCCTGGTC 
CTGGCCGAGAAGAACGTGGCGGTAGAGGTGATCGATATCGAGGCGGGCCGCTGTCCGCCGATGCTGGCGGAG 
GTCAACCCCTACGGCAGCGTACCGACCCTGGTCGATCGCGACCTGGCGCTGTATGAGCCGACGGTGGTCATG 
GAATACCTCGACGAACGCTATCCGCATCCGCCGCTGCTGCCCGATTATCCGGTGAAGCGTGCCAACAGCCGC 
CTGTTGATCCATCGTATCCAGCGGGACTGGTGTTCCCTGGTGGACCGGATCCTGGATGCGCGCAGCAAGGAG 
GCCGAGCGGGTCCAGGCGCGCAAGGAGTTGCGCGAGAGCCTGACCGGGGTTTCCCCGCTGTTCGCCGACAAG 
GCATATTTCCTCAGCGAGGATTTCAGCCTGGTGGATTGCTGCCTGTTGCCGATCCTCTGGCGTCTGCCGCTG 
CTGGGCATCGAGTTGCCGCGGCAGGCCAAGCCGTTGCTCGACTACATGGAGCGCGGATTCGCCCGGGAAAGC 
TTCCGGGCCAGCCTGTCTTCCGTTGAACGAGATATGCGTTAA