ViewGene
Locus Name:
GeneID:
Location:

GeneID:3731
TrivialName: PA14_70370
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_70370
PAO1 Ortholog Locus:PA5331
Sequence Length:642
Protein Length:213
Start: 6274959
Stop: 6274318
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08716.1|AE004945_10 (AE004945) orotate phosphoribosyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 213/213 (100%), Positives = 213/213 (100%)
Sequence:
ATGCAGGCGTATCAGCGCGATTTCATTCGTTTCGCCATCGAGCGCGGGGTTCTGCGCTTCGGTGAGTTCACC
CTCAAGTCCGGGCGCACCAGCCCCTATTTCTTCAATGCCGGCCTGTTCGACAGCGGCCTGGCGCTGGCCCGC
CTGGGACGCTTCTACGCCGAGGCGGTGATCGACAGCGGAATCGACTTCGACGTCCTGTTCGGCCCGGCCTAC
AAGGGCATTCCCCTGGCCGCCACCACCGCGGTGGCCCTCGCCGAGCAGCACCAGCGCGACCTGCCCTGGTGC
TTCAACCGCAAGGAAGCCAAGGACCACGGCGAAGGCGGCACGCTGGTCGGCGCGCCGCTGAGCGGCCGGGTG
CTGATCATCGACGACGTGATCACCGCCGGCACCGCGATCCGCGAAGTGATGCAGATCATCGACGCCCAGGGC
GCCAGGGCCGCGGGCGTGCTGATCGCGCTGAACCGCCAGGAGCGCGGCAAGGGCGAGCTGTCGGCGATCCAG
GAGGTCGAGCGCGACTTCGGCATGCCGGTGGTGAGCATCGTTTCCCTGGAGCAGGTGCTGGAATATCTTGCC
GAAGATGCTGAACTGAAGAAGCATCTGCCGGCGGTAGAGGCCTATCGCGCCCAATACGGTATCTGA
Translation:
MQAYQRDFIRFAIERGVLRFGEFTLKSGRTSPYFFNAGLFDSGLALARLGRFYAEAVIDSGIDFDVLFGPAY
KGIPLAATTAVALAEQHQRDLPWCFNRKEAKDHGEGGTLVGAPLSGRVLIIDDVITAGTAIREVMQIIDAQG
ARAAGVLIALNRQERGKGELSAIQEVERDFGMPVVSIVSLEQVLEYLAEDAELKKHLPAVEAYRAQYGI*
AnnotationID:3738GeneID:3731
AnnotatorUID: danlee
Modification Date/Time: 2006-01-31 13:01:17
Gene Name:pyrE
Confidence Code:2
GeneProduct:orotate phosphoribosyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0461
Secondary Functional Category(ies):
EC Number:2.4.2.10
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70856.1|AE016730_2 (AE016794) orotate phosphoribosyltransferase [Pseudomonas putida
           KT2440]
          Length = 213

 Score =  364 bits (935), Expect = e-100
 Identities = 183/213 (85%), Positives = 200/213 (93%)

>gb|AAO53634.1| (AE016856) orotate phosphoribosyltransferase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 213

 Score =  363 bits (933), Expect = e-100
 Identities = 183/213 (85%), Positives = 198/213 (92%)

>gb|AAM83690.1|AE013610_2 (AE013610) orotate phosphoribosyltransferase [Yersinia pestis KIM]
          Length = 215

 Score =  310 bits (795), Expect = 3e-84
 Identities = 147/213 (69%), Positives = 184/213 (86%)








 
Structural Features:
COG0461, PyrE, Orotate phosphoribosyltransferase 
[Nucleotide transport and metabolism]
CD-Length = 201 residues, 100.0% aligned
Score =  193 bits (492), Expect = 1e-50
Identities = 87/212 (41%), Positives = 129/212 (60%), Gaps = 14/212 (6%)








   
Genomic Context:






   
Comment:


















    ReferenceID:7465




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24607




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34773




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3731_cdd.html


    		




 
URL:

















    ReferenceID:35696




  
Author/Investigator(s):
        MacGregor CH, Arora SK, Hager PW, Dail MB, Phibbs PV Jr.





  
Title:
        The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product.




  
PubMed:
        8824606


MedLine:
        




    
Source:
J Bacteriol. 1996 Oct;178(19):5627-35.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8824606

















    ReferenceID:35697




  
Author/Investigator(s):
        Poulsen P, Jensen KF, Valentin-Hansen P, Carlsson P, Lundberg LG.





  
Title:
        Nucleotide sequence of the Escherichia coli pyrE gene and of the DNA in front of the protein-coding region.




  
PubMed:
        6349999


MedLine:
        




    
Source:
Eur J Biochem. 1983 Sep 15;135(2):223-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6349999

















    ReferenceID:35698




  
Author/Investigator(s):
        Vonstein V, Johnson SP, Yu H, Casadaban MJ, Pagratis NC, Weber JM, Demirjian DC.





  
Title:
        Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus.




  
PubMed:
        7635839


MedLine:
        




    
Source:
J Bacteriol. 1995 Aug;177(15):4540-3.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7635839









           
Homologs By Global Alignment 

           

       



     
Gene ID:3731
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5945
                           PA5331_tr
                           translation of PA5331
                           213
                           99.99
                           99.99
                           0
                           1073.0
     

     
     
166158
                           gb|AAG08716.1|AE004945_10
                           (AE004945) orotate phosphoribosyltransferase [Pseudomonas aeruginosa PAO1]
                           213
                           99.99
                           99.99
                           0
                           1073.0
     

     
     
166159
                           gb|AAC44427.1|
                           (U38241) orotate phosphoribosyl transferase [Pseudomonas aeruginosa]
                           213
                           99.53
                           99.99
                           0
                           1069.0
     

     
     
166160
                           gb|AAN70856.1|AE016730_2
                           (AE016794) orotate phosphoribosyltransferase [Pseudomonas putida KT2440]
                           213
                           85.91
                           93.89
                           0
                           935.0
     

     
     
166161
                           gb|AAO53634.1|
                           (AE016856) orotate phosphoribosyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           213
                           85.91
                           92.95
                           0
                           933.0
     

     






      

          
GC ORFID: 46733How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5331Glimmer Score: 

          
Start: 5538648Stop: 5538007

          
Length: 642


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5331 translation of PA5331Homolog Bit Score 417.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3143 (ORF: BLASTX 362.0), t_PA5331 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAGGCGTATCAGCGCGATTTCATTCGTTTCGCCATCGAGCGCGGGGTTCTGCGCTTCGGTGAGTTCACC 
CTCAAGTCCGGGCGCACCAGCCCCTATTTCTTCAATGCCGGCCTGTTCGACAGCGGCCTGGCGCTGGCCCGC 
CTGGGACGCTTCTACGCCGAGGCGGTGATCGACAGCGGAATCGACTTCGACGTCCTGTTCGGCCCGGCCTAC 
AAGGGCATTCCCCTGGCCGCCACCACCGCGGTGGCCCTCGCCGAGCAGCACCAGCGCGACCTGCCCTGGTGC 
TTCAACCGCAAGGAAGCCAAGGACCACGGCGAAGGCGGCACGCTGGTCGGCGCGCCGCTGAGCGGCCGGGTG 
CTGATCATCGACGACGTGATCACCGCCGGCACCGCGATCCGCGAAGTGATGCAGATCATCGACGCCCAGGGC 
GCCAGGGCCGCGGGCGTGCTGATCGCGCTGAACCGCCAGGAGCGCGGCAAGGGCGAGCTGTCGGCGATCCAG 
GAGGTCGAGCGCGACTTCGGCATGCCGGTGGTGAGCATCGTTTCCCTGGAGCAGGTGCTGGAATATCTTGCC 
GAAGATGCTGAACTGAAGAAGCATCTGCCGGCGGTAGAGGCCTATCGCGCCCAATACGGTATCTGA