ViewGene
Locus Name:
GeneID:
Location:

GeneID:3719
TrivialName: PA14_30650
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_30650
PAO1 Ortholog Locus:PA2586
Sequence Length:645
Protein Length:214
Start: 2655504
Stop: 2656148
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05974.1|AE004687_3 (AE004687) response regulator GacA [Pseudomonas aeruginosa PAO1]
Identities = 214/214 (100%), Positives = 214/214 (100%)
Sequence:
GTGATTAAGGTGCTGGTGGTCGACGACCACGATCTGGTACGCACCGGTATTACCCGCATGCTGGCCGACATC
GAAGGCTTGCAAGTGGTCGGCCAGGCCGACTGCGGTGAAGACTGTCTGAAACTGGCCCGCGAGCTGAAGCCG
GATGTCGTCCTGATGGACGTGAAGATGCCCGGTATCGGCGGCCTGGAGGCGACCCGCAAGCTGCTGCGCAGC
CAGCCCGACATCAAGGTCGTGGTAGTCACCGTCTGCGAAGAGGATCCGTTCCCCACCCGCCTCATGCAGGCC
GGCGCCGCCGGCTACATGACCAAGGGCGCGGGGCTGGAGGAAATGGTCCAGGCTATTCGCCAGGTCTTCGCC
GGCCAGCGCTATATCAGCCCGCAGATCGCCCAGCAACTGGCGCTGAAGTCCTTCCAGCCGCAGCAGCACGAT
TCCCCCTTCGATTCGCTGTCCGAGCGCGAGATCCAGATCGCCCTGATGATCGCCAACTGCCACAAGGTGCAG
AGCATCTCCGACAAGCTGTGCCTGTCGCCGAAGACCGTGAATACCTATCGCTACCGCATCTTCGAGAAGCTC
TCGATCACCAGCGACGTGGAGCTGGCGCTGCTCGCCGTCCGCCACGGCATGGTCGATGCCGCCAGCTAG
Translation:
MIKVLVVDDHDLVRTGITRMLADIEGLQVVGQADCGEDCLKLARELKPDVVLMDVKMPGIGGLEATRKLLRS
QPDIKVVVVTVCEEDPFPTRLMQAGAAGYMTKGAGLEEMVQAIRQVFAGQRYISPQIAQQLALKSFQPQQHD
SPFDSLSEREIQIALMIANCHKVQSISDKLCLSPKTVNTYRYRIFEKLSITSDVELALLAVRHGMVDAAS*
AnnotationID:7GeneID:3719
AnnotatorUID: diggins
Modification Date/Time: 2006-01-22 10:10:19
Gene Name:gacA
Confidence Code:1
GeneProduct:response regulator GacA
Cell Localization:(nucleus?) Unclassified
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(25) Transcriptional regulators
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2197
Secondary Functional Category(ies):signal transduction mechanisms
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO92275.1| (AY251005) response regulator GacA [Pseudomonas fluorescens]
          Length = 214

 Score =  412 bits (1060), Expect = e-115
 Identities = 210/212 (99%), Positives = 212/212 (100%)

>gb|AAO56514.1| (AE016866) DNA-binding response regulator GacA [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 214

 Score =  373 bits (958), Expect = e-103
 Identities = 192/214 (89%), Positives = 201/214 (93%)

>gb|AAC74981.1| (AE000284) putative 2-component transcriptional regulator
           [Escherichia coli K12]
          Length = 218

 Score =  259 bits (663), Expect = 6e-69
 Identities = 128/212 (60%), Positives = 167/212 (78%)






 
Structural Features:
COG2197, CitB, Response regulator containing a CheY-like receiver domain 
and an HTH DNA-binding domain 
[Signal transduction mechanisms / Transcription].
CD-Length = 211 residues, 100.0% aligned
Score =  205 bits (522), Expect = 4e-54
Identities = 93/211 (44%), Positives = 136/211 (64%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14903




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24583




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:38259




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3719_cdd.html


    		




 
URL:

















    ReferenceID:38260




  
Author/Investigator(s):
        Reimmann C, Beyeler M, Latifi A, Winteler H, Foglino M, Lazdunski A, Haas D.





  
Title:
        The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase.




  
PubMed:
        9159518


MedLine:
        




    
Source:
Mol Microbiol. 1997 Apr;24(2):309-19.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9159518&query_hl=15&itool=pubmed_DocSum









           
Homologs By Global Alignment 

           

       



     
Gene ID:3719
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5931
                           PA2586_tr
                           translation of PA2586
                           214
                           99.99
                           99.99
                           0
                           1074.0
     

     
     
165927
                           gb|AAG05974.1|AE004687_3
                           (AE004687) response regulator GacA [Pseudomonas aeruginosa PAO1]
                           214
                           99.53
                           99.99
                           0
                           1071.0
     

     
     
165928
                           gb|AAC45328.1|
                           (U89528) response regulator GacA [Pseudomonas aeruginosa]
                           214
                           99.53
                           99.99
                           0
                           1071.0
     

     
     
165931
                           gb|AAA68948.1|
                           (U27988) GacA [Pseudomonas aeruginosa]
                           214
                           99.06
                           99.53
                           1
                           1057.0
     

     
     
165929
                           gb|AAO92275.1|
                           (AY251005) response regulator GacA [Pseudomonas fluorescens]
                           214
                           97.66
                           99.06
                           0
                           1055.0
     

     






      

          
GC ORFID: 46721How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2586Glimmer Score: 

          
Start: 2298422Stop: 2297778

          
Length: 645


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA2586 translation of PA2586Homolog Bit Score 418.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6343 (ORF: BLASTX 372.0), t_PA2586 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGATTAAGGTGCTGGTGGTCGACGACCACGATCTGGTACGCACCGGTATTACCCGCATGCTGGCCGACATC 
GAAGGCTTGCAAGTGGTCGGCCAGGCCGACTGCGGTGAAGACTGTCTGAAACTGGCCCGCGAGCTGAAGCCG 
GATGTCGTCCTGATGGACGTGAAGATGCCCGGTATCGGCGGCCTGGAGGCGACCCGCAAGCTGCTGCGCAGC 
CAGCCCGACATCAAGGTCGTGGTAGTCACCGTCTGCGAAGAGGATCCGTTCCCCACCCGCCTCATGCAGGCC 
GGCGCCGCCGGCTACATGACCAAGGGCGCGGGGCTGGAGGAAATGGTCCAGGCTATTCGCCAGGTCTTCGCC 
GGCCAGCGCTATATCAGCCCGCAGATCGCCCAGCAACTGGCGCTGAAGTCCTTCCAGCCGCAGCAGCACGAT 
TCCCCCTTCGATTCGCTGTCCGAGCGCGAGATCCAGATCGCCCTGATGATCGCCAACTGCCACAAGGTGCAG 
AGCATCTCCGACAAGCTGTGCCTGTCGCCGAAGACCGTGAATACCTATCGCTACCGCATCTTCGAGAAGCTC 
TCGATCACCAGCGACGTGGAGCTGGCGCTGCTCGCCGTCCGCCACGGCATGGTCGATGCCGCCAGCTAG