ViewGene
Locus Name:
GeneID:
Location:

GeneID:370
TrivialName: PA14_12060
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_12060
PAO1 Ortholog Locus:PA4003
Sequence Length:1941
Protein Length:646
Start: 1040914
Stop: 1042854
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07390.1|AE004817_14 (AE004817) penicillin-binding protein 2 [Pseudomonas aeruginosa PAO1]
Identities = 646/646 (100%), Positives = 646/646 (100%)
Sequence:
ATGCCGCAGCCGATCCACCTGAAAGACCACGAGAAAGACGCCCGCCTGGTGCGTCGGCGCGTCATCGTCGGG
GCGGTGGCGGTGGTCTTGCTGACCCTGGTGCTGGTGGCGCGCATGTACCACCTGCAGGTGACCCAGTACGAG
TACCATTCGACGCTGTCGGAGAACAACCGGGTCCATGTGCAGCCGATCCCGCCGACCCGCGGGATCATCTTC
GACCGCAACGGCGTGATCATCGCCGACAACCGGCCGAGCTTCAGCCTGACCATCACCCGCGAACGCACCGAG
GACCTGCAGAAGACCCTCGACACGCTGGTGGAGATCCTCGGCCTGACCGAGGACGACCGGGCCATCTTCGAG
AAGCGCATGAAGCAGGGGCGGCGTCCGTTCGAGCCGGTTCCCATCATGTTCGAACTCAACGAGGAACAGATC
GCCCGCATCGCGGTGAACCAGTTCCGCCTGCCGGGCGTCGACGTGGCGACCCAGTTCGTCCGCCACTACCCG
CTGAAGGAGCACTTCGCCCATTCGGTCGGGTACGTCGGGCGGATCAACGAGCAGGAACTGAAGAACCTCGAC
CCGATCAACTACAGCGGCACCCACCATATCGGCAAGACCGGCATCGAGCGCTTCTACGAGAGCGAGCTGCAC
GGCACCGTTGGCTACGAGGAAGTCGAGACCAACGCCCGCGGGCGGGTGCTGCGGGTGCTCAAGCGCACCGAC
CCGATTCCCGGCAAGGACATCGTCCTGAGCATCGATAGCCGCCTGCAGGAGGCCGCCGAGAACGCCCTGGCC
GGGCGGCGGGGCGCCATCGTGGCGATCCAGCCGTCCACCGGCGACGTGCTGGCGATGGTCAGCCAGCCGAGC
TACGACCCCAACCTGTTCGTCACCGGCATCAGCTTCAAGGCCTATGCCGAGCTGCGCGACTCCATCGACCGG
CCGCTGTACAACCGCGTCCTGCGCGGGCTCTATCCGCCGGGCTCGACGGTGAAACCGGCGGTGGCGCTGGCC
GGCCTCGACGCCGGGGTGGTCACGCCGACCTCGCGGGTCTTCGACCCCGGCTACTACCAGTTGCCCAACTAC
GACCACAAGTACCGTAACTGGAACCGCTACGGCGATGGCTGGGTGAGCCTGGAGTCGGCGATCTACCGGTCC
AACGACACCTATTTCTACGACCTCGCGCACAAGCTGGGGATCGACCGCCTGCACGCCTTCATGAGTCGCTTC
GGTTTCGGCCAGAAAGTCGCCCTGGACATGTTCGGCGAAGCCGACGGGCTGATGCCCTCGCGCGAATGGAAG
CGCAAGACCCGCCGCCAGGTCTGGTACCCGGGGGAGACCCTGATCCTCGGCATCGGCCAGGGCTACATGCAG
GCGACGCCGATCCAGTTGGCGCAGATGACCGCGCTGCTGGCCAACAAGGGCCACTGGATCCGCCCGCACCTG
GCCAAGACCATCGACGGCCAGCCGCCGGTCGACCCCGACCCGATGCCCGACATCGTGCTGCGCGACCCGGCC
AACTGGGACCGCGTCGACTACGGCATGCAGCAGGTGGTGCATGGCGCCCGTGGCACGGCGCGCAAGGTCGGC
GCCACCTCGGCCTACCTGATCGCCGGCAAGAGCGGTACCGCCCAGGTGGTGGCGATCAAGCAGAACGAGCGC
TACGACCGCTCCAAGCTGCTCGAGCGGCACCGCGACCACGCCCTGTTCGTCGGCTTCGCGCCGGCCAACAAC
CCGCAGATCGCGGTGGCGGTGATGGTGGAGAACGGCGAGTCCGGCTCCGGCGTCGCCGCCCCGGTGGTCAAG
CAGGTGATGGACGCCTGGCTGCTCGACGAGCACGGCAAGCTCAAGGCCGAATACGCGGAACCCGTCGCGCCG
CTCGCGGCGGCGGTGGCCAAGCCCGAACCCACGGCAGCCGAGCCGGAGGCGCCCGCCCTTGAACAGTAA
Translation:
MPQPIHLKDHEKDARLVRRRVIVGAVAVVLLTLVLVARMYHLQVTQYEYHSTLSENNRVHVQPIPPTRGIIF
DRNGVIIADNRPSFSLTITRERTEDLQKTLDTLVEILGLTEDDRAIFEKRMKQGRRPFEPVPIMFELNEEQI
ARIAVNQFRLPGVDVATQFVRHYPLKEHFAHSVGYVGRINEQELKNLDPINYSGTHHIGKTGIERFYESELH
GTVGYEEVETNARGRVLRVLKRTDPIPGKDIVLSIDSRLQEAAENALAGRRGAIVAIQPSTGDVLAMVSQPS
YDPNLFVTGISFKAYAELRDSIDRPLYNRVLRGLYPPGSTVKPAVALAGLDAGVVTPTSRVFDPGYYQLPNY
DHKYRNWNRYGDGWVSLESAIYRSNDTYFYDLAHKLGIDRLHAFMSRFGFGQKVALDMFGEADGLMPSREWK
RKTRRQVWYPGETLILGIGQGYMQATPIQLAQMTALLANKGHWIRPHLAKTIDGQPPVDPDPMPDIVLRDPA
NWDRVDYGMQQVVHGARGTARKVGATSAYLIAGKSGTAQVVAIKQNERYDRSKLLERHRDHALFVGFAPANN
PQIAVAVMVENGESGSGVAAPVVKQVMDAWLLDEHGKLKAEYAEPVAPLAAAVAKPEPTAAEPEAPALEQ*
AnnotationID:379GeneID:370
AnnotatorUID: urbach
Modification Date/Time: 2004-09-13 17:05:27
Gene Name:pbpA
Confidence Code:2
GeneProduct:FtsI / penicillin-binding protein 2
Cell Localization:(6) Outer membrane protein
Synonyms:
Cell Localization Confidence Code:2
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0768,pfam00905,pfam03717
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gnl|CDD|10636 COG0768, FtsI, Cell division protein FtsI/penicillin-binding protein 2
[Cell envelope biogenesis, outer membrane]
Length = 599
Score =  513 bits (1323), Expect = e-146
Identities = 249/631 (39%), Positives = 336/631 (53%), Gaps = 52/631 (8%)






 
Structural Features:
gb|AAG07390.1|AE004817_14 (AE004817) penicillin-binding protein 2
[Pseudomonas aeruginosa PAO1]
Length = 646
Score = 1300 bits (3363), Expect = 0.0
Identities = 646/646 (100%), Positives = 646/646 (100%)






   
Genomic Context:






   
Comment:


















    ReferenceID:743




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17885




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34287




  
Author/Investigator(s):
        S Asoh, H Matsuzawa, M Matsuhashi, and T Ohta





  
Title:
        Molecular cloning and characterization of the genes (pbpA and rodA) responsible for the rod shape of Escherichia coli K-12: analysis of gene expression with transposon Tn5 mutagenesis and protein synthesis directed by constructed plasmids.




  
PubMed:
        6300030


MedLine:
        




    
Source:
J Bacteriol. 1983 April; 154 (1): 1016






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6300030









           
Homologs By Global Alignment 

           

       



     
Gene ID:370
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
617
                           PA4003_tr
                           translation of PA4003
                           646
                           99.99
                           99.99
                           0
                           3363.0
     

     
     
28985
                           gb|AAG07390.1|AE004817_14
                           (AE004817) penicillin-binding protein 2 [Pseudomonas aeruginosa PAO1]
                           646
                           99.99
                           99.99
                           0
                           3363.0
     

     
     
28986
                           gb|AAD32230.1|AF147448_1
                           (AF147448) penicillin-binding protein 2 [Pseudomonas aeruginosa]
                           646
                           99.99
                           99.99
                           0
                           3363.0
     

     
     
28987
                           gb|AAO58254.1|
                           (AE016873) penicillin-binding protein [Pseudomonas syringae pv. tomato str. DC3000]
                           646
                           78.01
                           87.77
                           15
                           2679.0
     

     
     
28988
                           gb|AAN70376.1|AE016679_8
                           (AE016792) penicillin-binding protein 2 [Pseudomonas putida KT2440]
                           646
                           76.47
                           87.15
                           17
                           2657.0
     

     






      

          
GC ORFID: 43372How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4003Glimmer Score: 

          
Start: 3913024Stop: 3911084

          
Length: 1941


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4003 translation of PA4003Homolog Bit Score 1300.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5326 (ORF: BLASTX 1037.0), psyr_15may02_Scaffold1_revised_gene1279 (ORF: BLASTX 890.0), t_PA4003 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCGCAGCCGATCCACCTGAAAGACCACGAGAAAGACGCCCGCCTGGTGCGTCGGCGCGTCATCGTCGGG 
GCGGTGGCGGTGGTCTTGCTGACCCTGGTGCTGGTGGCGCGCATGTACCACCTGCAGGTGACCCAGTACGAG 
TACCATTCGACGCTGTCGGAGAACAACCGGGTCCATGTGCAGCCGATCCCGCCGACCCGCGGGATCATCTTC 
GACCGCAACGGCGTGATCATCGCCGACAACCGGCCGAGCTTCAGCCTGACCATCACCCGCGAACGCACCGAG 
GACCTGCAGAAGACCCTCGACACGCTGGTGGAGATCCTCGGCCTGACCGAGGACGACCGGGCCATCTTCGAG 
AAGCGCATGAAGCAGGGGCGGCGTCCGTTCGAGCCGGTTCCCATCATGTTCGAACTCAACGAGGAACAGATC 
GCCCGCATCGCGGTGAACCAGTTCCGCCTGCCGGGCGTCGACGTGGCGACCCAGTTCGTCCGCCACTACCCG 
CTGAAGGAGCACTTCGCCCATTCGGTCGGGTACGTCGGGCGGATCAACGAGCAGGAACTGAAGAACCTCGAC 
CCGATCAACTACAGCGGCACCCACCATATCGGCAAGACCGGCATCGAGCGCTTCTACGAGAGCGAGCTGCAC 
GGCACCGTTGGCTACGAGGAAGTCGAGACCAACGCCCGCGGGCGGGTGCTGCGGGTGCTCAAGCGCACCGAC 
CCGATTCCCGGCAAGGACATCGTCCTGAGCATCGATAGCCGCCTGCAGGAGGCCGCCGAGAACGCCCTGGCC 
GGGCGGCGGGGCGCCATCGTGGCGATCCAGCCGTCCACCGGCGACGTGCTGGCGATGGTCAGCCAGCCGAGC 
TACGACCCCAACCTGTTCGTCACCGGCATCAGCTTCAAGGCCTATGCCGAGCTGCGCGACTCCATCGACCGG 
CCGCTGTACAACCGCGTCCTGCGCGGGCTCTATCCGCCGGGCTCGACGGTGAAACCGGCGGTGGCGCTGGCC 
GGCCTCGACGCCGGGGTGGTCACGCCGACCTCGCGGGTCTTCGACCCCGGCTACTACCAGTTGCCCAACTAC 
GACCACAAGTACCGTAACTGGAACCGCTACGGCGATGGCTGGGTGAGCCTGGAGTCGGCGATCTACCGGTCC 
AACGACACCTATTTCTACGACCTCGCGCACAAGCTGGGGATCGACCGCCTGCACGCCTTCATGAGTCGCTTC 
GGTTTCGGCCAGAAAGTCGCCCTGGACATGTTCGGCGAAGCCGACGGGCTGATGCCCTCGCGCGAATGGAAG 
CGCAAGACCCGCCGCCAGGTCTGGTACCCGGGGGAGACCCTGATCCTCGGCATCGGCCAGGGCTACATGCAG 
GCGACGCCGATCCAGTTGGCGCAGATGACCGCGCTGCTGGCCAACAAGGGCCACTGGATCCGCCCGCACCTG 
GCCAAGACCATCGACGGCCAGCCGCCGGTCGACCCCGACCCGATGCCCGACATCGTGCTGCGCGACCCGGCC 
AACTGGGACCGCGTCGACTACGGCATGCAGCAGGTGGTGCATGGCGCCCGTGGCACGGCGCGCAAGGTCGGC 
GCCACCTCGGCCTACCTGATCGCCGGCAAGAGCGGTACCGCCCAGGTGGTGGCGATCAAGCAGAACGAGCGC 
TACGACCGCTCCAAGCTGCTCGAGCGGCACCGCGACCACGCCCTGTTCGTCGGCTTCGCGCCGGCCAACAAC 
CCGCAGATCGCGGTGGCGGTGATGGTGGAGAACGGCGAGTCCGGCTCCGGCGTCGCCGCCCCGGTGGTCAAG 
CAGGTGATGGACGCCTGGCTGCTCGACGAGCACGGCAAGCTCAAGGCCGAATACGCGGAACCCGTCGCGCCG 
CTCGCGGCGGCGGTGGCCAAGCCCGAACCCACGGCAGCCGAGCCGGAGGCGCCCGCCCTTGAACAGTAA