ViewGene
Locus Name:
GeneID:
Location:

GeneID:364
TrivialName: PA14_28650
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_28650
PAO1 Ortholog Locus:PA2744
Sequence Length:1923
Protein Length:640
Start: 2467219
Stop: 2469141
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06132.1|AE004702_9 (AE004702) threonyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
Identities = 640/640 (100%), Positives = 640/640 (100%)
Sequence:
ATGCCCATCATTACTCTTCCCGACGGCAGTCAACGTTCCTTCGATCACCCGGTCTCCGTGGCCGAGGTGGCC
CAATCCATCGGCGCAGGCCTGGCCAAGGCGACCCTCGCCGGCAAGGTCGACGGCCGCCTGGTCGACGCCTGC
GACACCATCGATCGCGACGCGACCCTGCAGATCATCACGCCCAAGGACGAGGAAGGACTGGAGATCATCCGC
CACTCCTGCGCCCACCTGGTCGGCCATGCGGTCAAGCAGCTCTATCCGACCGCGAAGATGGTCATCGGCCCG
GTGATCGAGGAAGGCTTCTACTACGACATCTTCTTCGAGCGCCCCTTCACCCCCGAGGACATGGCGGCGATC
CAGCAGCGCATGCGCGAGCTGATCGACAAGGACTACGACGTGATCAAGAAGATGACCCCGCGCGCCGAGGTC
ATCGAGCTGTTCAAGTCCCGTGGCGAAGACTACAAGCTGCGCCTGATCGACGACATGCCGGACGAGAAGGCC
ATGGGCCTGTACTTCCATGAGGAGTACGTGGACATGTGCCGCGGCCCGCACGTGCCGAACACCCGCTTCCTC
AAGGCGTTCCAGCTGACCAAGATTTCCGGCGCCTACTGGCGCGGCGACTCGAAGAACGAGCAGTTGCAACGC
ATCTACGGCACCGCCTGGGCCGACAAGAAGCAACTGGCGGCCTACATCCAGCGCATCGAAGAGGCCGAGAAG
CGCGACCATCGCCGCATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCGCCGGGCATGGTGTTC
TGGCACCCGAATGGCTGGAGCGTCTACCAGGTGCTCGAGCAGTACATGCGCAAGGTCCAGCGCGACCATGGC
TATGTCGAAGTGCGTACCCCGCAGGTGGTCGACCGCATCCTCTGGGAGCGTTCGGGCCACTGGTCGAACTAC
GCCGAGAACATGTTCACCACCTCCTCGGAAAGCCGCGACTACGCGGTCAAGCCGATGAACTGCCCGTGCCAC
GTGCAGATCTTCAACCAGGGCCTGAAGTCCTACCGCGACCTGCCGCTGCGCCTCGCCGAGTTCGGCGCCTGC
CACCGCAACGAGCCGTCCGGCGCGCTGCACGGGATCATGCGCGTACGCGGCTTCACCCAGGACGACGCGCAT
ATCTTCTGCACCGAAGAGCAGGTGAAGAAGGAAGCGGCCGATTTCATCAAGCTGACCTTGCAGGTCTACCGC
GACTTCGGTTTCACCGACATCGCCATGAAGCTGTCGACCCGTCCGGCCAAGCGCGTCGGTTCCGACGAGCTG
TGGGATCGCGCCGAAGGCGCGCTGGCCGATGCGCTGAACGAATCCGGCCTGGCCTGGGAATACCAGCCGGGC
GAGGGCGCGTTCTATGGGCCAAAGATCGAGTTCACCCTGAAGGACTGCCTCGGCCGTAACTGGCAGTGCGGC
ACCCTGCAGTACGACCCGAACCTGCCGGAGCGCCTGGACGCCAGCTACATCGCCGAGGACAACAACCGTAAG
CGCCCGGTGATGCTGCACCGTGCGATCCTCGGGTCCTTCGAGCGCTTCATCGGCATGCTCATCGAGCACTAC
GCCGGAGCCTTCCCGGCCTGGCTGGCGCCGACCCAGGCGGTGGTGATGAATATCACCGACAAGCAGGCCGAT
TTCGCCGCCGAGGTGGTGCGGATCCTCGGGGAAAGCGGATTCCGTGCCAAGTCCGACTTGAGAAACGAGAAG
ATCGGCTTTAAAATCCGCGAGCATACTTTGCTCAAGGTTCCCTATCTCTTGGTTATTGGAGATCGGGAAGTT
GAATCGAAGGCCGTCGCGGTGCGTACGCGCGAAGGGGAAGACCTGGGCTCCATGCCCGTCACCCAGTTCGCT
GAGCTGTTGGCACAGGCGGTTTCCCGGCGTGGTCGCCAAGACTCGGAGTAA
Translation:
MPIITLPDGSQRSFDHPVSVAEVAQSIGAGLAKATLAGKVDGRLVDACDTIDRDATLQIITPKDEEGLEIIR
HSCAHLVGHAVKQLYPTAKMVIGPVIEEGFYYDIFFERPFTPEDMAAIQQRMRELIDKDYDVIKKMTPRAEV
IELFKSRGEDYKLRLIDDMPDEKAMGLYFHEEYVDMCRGPHVPNTRFLKAFQLTKISGAYWRGDSKNEQLQR
IYGTAWADKKQLAAYIQRIEEAEKRDHRRIGKQLDLFHLQEEAPGMVFWHPNGWSVYQVLEQYMRKVQRDHG
YVEVRTPQVVDRILWERSGHWSNYAENMFTTSSESRDYAVKPMNCPCHVQIFNQGLKSYRDLPLRLAEFGAC
HRNEPSGALHGIMRVRGFTQDDAHIFCTEEQVKKEAADFIKLTLQVYRDFGFTDIAMKLSTRPAKRVGSDEL
WDRAEGALADALNESGLAWEYQPGEGAFYGPKIEFTLKDCLGRNWQCGTLQYDPNLPERLDASYIAEDNNRK
RPVMLHRAILGSFERFIGMLIEHYAGAFPAWLAPTQAVVMNITDKQADFAAEVVRILGESGFRAKSDLRNEK
IGFKIREHTLLKVPYLLVIGDREVESKAVAVRTREGEDLGSMPVTQFAELLAQAVSRRGRQDSE*
AnnotationID:373GeneID:364
AnnotatorUID: diggins
Modification Date/Time: 2005-04-05 08:08:45
Gene Name:thrS
Confidence Code:2
GeneProduct:threonyl-tRNA synthetase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0441
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:6.1.1.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68077.1|AE016439_12 (AE016783) threonyl-tRNA synthetase [Pseudomonas putida KT2440]
          Length = 640

 Score = 1201 bits (3106), Expect = 0.0
 Identities = 576/640 (90%), Positives = 614/640 (95%)

>gb|AAO55889.1| (AE016864) threonyl-tRNA synthetase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 640

 Score = 1127 bits (2916), Expect = 0.0
 Identities = 537/640 (83%), Positives = 596/640 (93%)

>gb|AAM37443.1| (AE011899) threonyl-tRNA synthetase [Xanthomonas axonopodis pv.
           citri str. 306]
          Length = 634

 Score =  939 bits (2426), Expect = 0.0
 Identities = 449/625 (71%), Positives = 520/625 (83%), Gaps = 1/625 (0%)






 
Structural Features:
COG0441, ThrS, Threonyl-tRNA synthetase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 589 residues, 100.0% aligned
Score =  801 bits (2071), Expect = 0.0
Identities = 331/608 (54%), Positives = 423/608 (69%), Gaps = 21/608 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:731




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17873




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36294




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:364_cdd.html


    		




 
URL:

















    ReferenceID:36383




  
Author/Investigator(s):
        Kolibachuk D, Rouhbakhsh D, Baumann P.





  
Title:
        Aromatic amino acid biosynthesis in Buchnera aphidicola (endosymbiont of aphids): cloning and sequencing of a DNA fragment containing aroH-thrS-infC-rpmI-rplT.




  
PubMed:
        7766160


MedLine:
        




    
Source:
Curr Microbiol. 1995 May;30(5):313-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7766160

















    ReferenceID:36390




  
Author/Investigator(s):
        Mayaux JF, Fayat G, Fromant M, Springer M, Grunberg-Manago M, Blanquet S.





  
Title:
        Structural and transcriptional evidence for related thrS and infC expression.




  
PubMed:
        6353409


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1983 Oct;80(20):6152-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6353409









           
Homologs By Global Alignment 

           

       



     
Gene ID:364
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
609
                           PA2744_tr
                           translation of PA2744
                           640
                           99.99
                           99.99
                           0
                           3374.0
     

     
     
28796
                           gb|AAG06132.1|AE004702_9
                           (AE004702) threonyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
                           640
                           99.99
                           99.99
                           0
                           3374.0
     

     
     
28797
                           gb|AAN68077.1|AE016439_12
                           (AE016783) threonyl-tRNA synthetase [Pseudomonas putida KT2440]
                           640
                           90.00
                           95.93
                           0
                           3106.0
     

     
     
28798
                           gb|AAO55889.1|
                           (AE016864) threonyl-tRNA synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           640
                           83.90
                           93.12
                           0
                           2916.0
     

     
     
28799
                           gb|AAM37443.1|
                           (AE011899) threonyl-tRNA synthetase [Xanthomonas axonopodis pv. citri str. 306]
                           643
                           70.29
                           81.33
                           12
                           2424.0
     

     






      

          
GC ORFID: 43366How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2744Glimmer Score: 

          
Start: 2486707Stop: 2484785

          
Length: 1923


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2744 translation of PA2744Homolog Bit Score 1304.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1163 (ORF: BLASTX 1126.0), t_PA2744 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCATCATTACTCTTCCCGACGGCAGTCAACGTTCCTTCGATCACCCGGTCTCCGTGGCCGAGGTGGCC 
CAATCCATCGGCGCAGGCCTGGCCAAGGCGACCCTCGCCGGCAAGGTCGACGGCCGCCTGGTCGACGCCTGC 
GACACCATCGATCGCGACGCGACCCTGCAGATCATCACGCCCAAGGACGAGGAAGGACTGGAGATCATCCGC 
CACTCCTGCGCCCACCTGGTCGGCCATGCGGTCAAGCAGCTCTATCCGACCGCGAAGATGGTCATCGGCCCG 
GTGATCGAGGAAGGCTTCTACTACGACATCTTCTTCGAGCGCCCCTTCACCCCCGAGGACATGGCGGCGATC 
CAGCAGCGCATGCGCGAGCTGATCGACAAGGACTACGACGTGATCAAGAAGATGACCCCGCGCGCCGAGGTC 
ATCGAGCTGTTCAAGTCCCGTGGCGAAGACTACAAGCTGCGCCTGATCGACGACATGCCGGACGAGAAGGCC 
ATGGGCCTGTACTTCCATGAGGAGTACGTGGACATGTGCCGCGGCCCGCACGTGCCGAACACCCGCTTCCTC 
AAGGCGTTCCAGCTGACCAAGATTTCCGGCGCCTACTGGCGCGGCGACTCGAAGAACGAGCAGTTGCAACGC 
ATCTACGGCACCGCCTGGGCCGACAAGAAGCAACTGGCGGCCTACATCCAGCGCATCGAAGAGGCCGAGAAG 
CGCGACCATCGCCGCATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCGCCGGGCATGGTGTTC 
TGGCACCCGAATGGCTGGAGCGTCTACCAGGTGCTCGAGCAGTACATGCGCAAGGTCCAGCGCGACCATGGC 
TATGTCGAAGTGCGTACCCCGCAGGTGGTCGACCGCATCCTCTGGGAGCGTTCGGGCCACTGGTCGAACTAC 
GCCGAGAACATGTTCACCACCTCCTCGGAAAGCCGCGACTACGCGGTCAAGCCGATGAACTGCCCGTGCCAC 
GTGCAGATCTTCAACCAGGGCCTGAAGTCCTACCGCGACCTGCCGCTGCGCCTCGCCGAGTTCGGCGCCTGC 
CACCGCAACGAGCCGTCCGGCGCGCTGCACGGGATCATGCGCGTACGCGGCTTCACCCAGGACGACGCGCAT 
ATCTTCTGCACCGAAGAGCAGGTGAAGAAGGAAGCGGCCGATTTCATCAAGCTGACCTTGCAGGTCTACCGC 
GACTTCGGTTTCACCGACATCGCCATGAAGCTGTCGACCCGTCCGGCCAAGCGCGTCGGTTCCGACGAGCTG 
TGGGATCGCGCCGAAGGCGCGCTGGCCGATGCGCTGAACGAATCCGGCCTGGCCTGGGAATACCAGCCGGGC 
GAGGGCGCGTTCTATGGGCCAAAGATCGAGTTCACCCTGAAGGACTGCCTCGGCCGTAACTGGCAGTGCGGC 
ACCCTGCAGTACGACCCGAACCTGCCGGAGCGCCTGGACGCCAGCTACATCGCCGAGGACAACAACCGTAAG 
CGCCCGGTGATGCTGCACCGTGCGATCCTCGGGTCCTTCGAGCGCTTCATCGGCATGCTCATCGAGCACTAC 
GCCGGAGCCTTCCCGGCCTGGCTGGCGCCGACCCAGGCGGTGGTGATGAATATCACCGACAAGCAGGCCGAT 
TTCGCCGCCGAGGTGGTGCGGATCCTCGGGGAAAGCGGATTCCGTGCCAAGTCCGACTTGAGAAACGAGAAG 
ATCGGCTTTAAAATCCGCGAGCATACTTTGCTCAAGGTTCCCTATCTCTTGGTTATTGGAGATCGGGAAGTT 
GAATCGAAGGCCGTCGCGGTGCGTACGCGCGAAGGGGAAGACCTGGGCTCCATGCCCGTCACCCAGTTCGCT 
GAGCTGTTGGCACAGGCGGTTTCCCGGCGTGGTCGCCAAGACTCGGAGTAA