ViewGene
Locus Name:
GeneID:
Location:

GeneID:3639
TrivialName: PA14_72450
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_72450
PAO1 Ortholog Locus:PA5489
Sequence Length:636
Protein Length:211
Start: 6454488
Stop: 6453853
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08874.1|AE004961_9 (AE004961) thiol:disulfide interchange protein DsbA [Pseudomonas aeruginosa PAO1]
Identities = 211/211 (100%), Positives = 211/211 (100%)
Sequence:
ATGCGTAACCTGATTCTCACCGCCATGCTGGCCATGGCCAGCCTGTTCGGCATGGCCGCCCAGGCCGACGAC
TATACCGCCGGCAAGGAATACGTCGAGCTGAGCAGCCCGGTGCCGGTGTCCCAGCCGGGCAAGATCGAAGTG
GTGGAACTGTTCTGGTATGGCTGCCCGCATTGCTACGCGTTCGAGCCGACCATCGTGCCGTGGAGCGAGAAG
CTGCCGGCAGATGTCCATTTCGTGCGCCTGCCTGCCCTGTTCGGCGGTATCTGGAACGTCCATGGGCAGATG
TTCCTGACCCTGGAAAGCATGGGTGTCGAGCATGACGTCCACAACGCCGTGTTCGAGGCGATCCACAAGGAG
CACAAGAAGCTCGCCACTCCGGAAGAGATGGCCGATTTCCTCGCCGGCAAGGGCGTGGACAAGGAAAAATTC
CTGAGCACCTATAATTCCTTTGCCATCAAGGGCCAGATGGAAAAGGCCAAGAAGCTGGCGATGGCCTACCAG
GTCACCGGCGTACCGACCATGGTGGTCAATGGCAAATACCGCTTCGACATCGGCTCCGCCGGTGGTCCGGAG
GAAACCCTCAAGCTGGCCGACTACCTGATCGAGAAAGAGCGCGCAGCGGCCAAGAAGTAG
Translation:
MRNLILTAMLAMASLFGMAAQADDYTAGKEYVELSSPVPVSQPGKIEVVELFWYGCPHCYAFEPTIVPWSEK
LPADVHFVRLPALFGGIWNVHGQMFLTLESMGVEHDVHNAVFEAIHKEHKKLATPEEMADFLAGKGVDKEKF
LSTYNSFAIKGQMEKAKKLAMAYQVTGVPTMVVNGKYRFDIGSAGGPEETLKLADYLIEKERAAAKK*
AnnotationID:3647GeneID:3639
AnnotatorUID: diggins
Modification Date/Time: 2004-07-19 11:11:22
Gene Name:dsbA
Confidence Code:2
GeneProduct:thiol:disulfide interchange protein DsbA
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:disulfide oxidoreductase
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO13005.1| (AY171618) probable thiol:disulfide interchange protein DsbA
           precursor [Pseudomonas fluorescens]
          Length = 213

 Score =  311 bits (797), Expect = 2e-84
 Identities = 153/210 (72%), Positives = 176/210 (83%), Gaps = 1/210 (0%)

>gb|AAB53016.1| (L76098) disulfide oxidoreductase [Azotobacter vinelandii]
          Length = 214

 Score =  308 bits (789), Expect = 1e-83
 Identities = 147/213 (69%), Positives = 176/213 (82%), Gaps = 5/213 (2%)

>gb|AAB92367.1| (AF036929) disulfide oxidoreductase [Pseudomonas syringae]
          Length = 214

 Score =  303 bits (776), Expect = 4e-82
 Identities = 143/211 (67%), Positives = 181/211 (85%), Gaps = 1/211 (0%)






 
Structural Features:






   
Genomic Context:






   
Comment:


















    ReferenceID:7281




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24423




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33239




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3639_cdd.html


    		




 
URL:

















    ReferenceID:35610




  
Author/Investigator(s):
        Kang JW, Jeong YJ, Kwon AR, Yun HJ, Kim DH, Choi EC.





  
Title:
        Cloning, sequence analysis, and characterization of the astA gene encoding an arylsulfate sulfotransferase from Citrobacter freundii.




  
PubMed:
        11534764


MedLine:
        




    
Source:
Arch Pharm Res. 2001 Aug;24(4):316-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11534764

















    ReferenceID:35611




  
Author/Investigator(s):
        Kang JW, Kwon AR, Kim DH, Choi EC.





  
Title:
        Cloning and sequencing of the astA gene encoding arylsulfate sulfotransferase from Salmonella typhimurium.




  
PubMed:
        11379783


MedLine:
        




    
Source:
Biol Pharm Bull. 2001 May;24(5):570-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11379783

















    ReferenceID:35612




  
Author/Investigator(s):
        Bardwell JC, McGovern K, Beckwith J.





  
Title:
        Identification of a protein required for disulfide bond formation in vivo.




  
PubMed:
        1934062


MedLine:
        




    
Source:
Cell. 1991 Nov 1;67(3):581-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1934062









           
Homologs By Global Alignment 

           

       



     
Gene ID:3639
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5828
                           PA5489_tr
                           translation of PA5489
                           211
                           99.99
                           99.99
                           0
                           1104.0
     

     
     
162626
                           gb|AAG08874.1|AE004961_9
                           (AE004961) thiol:disulfide interchange protein DsbA [Pseudomonas aeruginosa PAO1]
                           211
                           99.99
                           99.99
                           0
                           1104.0
     

     
     
162627
                           gb|AAD22452.1|AF116282_1
                           (AF116282) thiol:disulfide interchange protein DsbA precursor [Pseudomonas aeruginosa]
                           211
                           99.05
                           99.05
                           1
                           1094.0
     

     
     
162628
                           gb|AAB41795.1|
                           (U84726) DsbA [Pseudomonas aeruginosa]
                           211
                           99.05
                           99.05
                           1
                           1094.0
     

     
     
162629
                           gb|AAO13005.1|
                           (AY171618) probable thiol:disulfide interchange protein DsbA precursor [Pseudomonas fluorescens]
                           214
                           71.49
                           82.24
                           4
                           798.0
     

     






      

          
GC ORFID: 46641How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5489Glimmer Score: 

          
Start: 5718177Stop: 5717542

          
Length: 636


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA5489 translation of PA5489Homolog Bit Score 429.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3899 (ORF: BLASTX 302.0), t_PA5489 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGTAACCTGATTCTCACCGCCATGCTGGCCATGGCCAGCCTGTTCGGCATGGCCGCCCAGGCCGACGAC 
TATACCGCCGGCAAGGAATACGTCGAGCTGAGCAGCCCGGTGCCGGTGTCCCAGCCGGGCAAGATCGAAGTG 
GTGGAACTGTTCTGGTATGGCTGCCCGCATTGCTACGCGTTCGAGCCGACCATCGTGCCGTGGAGCGAGAAG 
CTGCCGGCAGATGTCCATTTCGTGCGCCTGCCTGCCCTGTTCGGCGGTATCTGGAACGTCCATGGGCAGATG 
TTCCTGACCCTGGAAAGCATGGGTGTCGAGCATGACGTCCACAACGCCGTGTTCGAGGCGATCCACAAGGAG 
CACAAGAAGCTCGCCACTCCGGAAGAGATGGCCGATTTCCTCGCCGGCAAGGGCGTGGACAAGGAAAAATTC 
CTGAGCACCTATAATTCCTTTGCCATCAAGGGCCAGATGGAAAAGGCCAAGAAGCTGGCGATGGCCTACCAG 
GTCACCGGCGTACCGACCATGGTGGTCAATGGCAAATACCGCTTCGACATCGGCTCCGCCGGTGGTCCGGAG 
GAAACCCTCAAGCTGGCCGACTACCTGATCGAGAAAGAGCGCGCAGCGGCCAAGAAGTAG