ViewGene

Locus Name:
GeneID:
Location:

GeneID: 3630

TrivialName: PA14_18410

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_18410

PAO1 Ortholog Locus: PA3550

Sequence Length: 651

Protein Length: 216

Start: 1580949

Stop: 1580299

Strand: -

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: gb|AAG06938.1|AE004776_1 (AE004776) alginate o-acetyltransferase AlgF [Pseudomonas aeruginosa PAO1] Identities = 215/216 (99%), Positives = 215/216 (99%)

Sequence:

ATGAACCCGATGACCCGCCGCCACACCTGGACCCGCCTGGCCTGCGCACTTTCCCTCGGCGTCGCCGCATTC
GCCGCGCAGGCCGACGAGGGCGCCCTGTACGGGCCCCAGGCACCCAAGGGCTCGGCCTTCGTCCGCGCCTAC
AACGCCGGCAACAGCGAACTCGACGTGTCGGTCGGCAGCACCAGCCTGAACGACGTCGCGCCGCTCGGTTCC
AGCGACTTCAAGTTCCTCCCCCCCGGCAGCTACACCGCCCAGGTCGGCCAGCAGAGCCTGCAGGTCAAGCTC
GACCCGGACAGCTACTACACCCTGGTCAGCCAGCCCGGCGGCAAGCCGCAGCTGGTGGCCGAGCCGCCGTTC
AAGAACAAGCAGAAGGCGCTGGTACGGGTGCAGAACCTCAGCGGCTCGAAGCTGACCCTGAAGACCGCCGAC
GGCAAGACCGACGTGGTCAAGGACGTCGGCCCGCAAAGCCACGGCGACCGCGAGATCAACCCGGTGAAGGTG
AACCTGGCGCTGTTCGACGGCAGCAAGAAGGTCAGCGACCTGAAGCCAGTCACCCTGGCCCGCGGCGAGGTG
GTCTGCCTGTACGTCACCGGCAGCGGCGGCAAGCTCGCGCCGGTCTGGGTCAAGCGCCCGGTGAAGGCGGAC
TGA

Translation:

MNPMTRRHTWTRLACALSLGVAAFAAQADEGALYGPQAPKGSAFVRAYNAGNSELDVSVGSTSLNDVAPLGS
SDFKFLPPGSYTAQVGQQSLQVKLDPDSYYTLVSQPGGKPQLVAEPPFKNKQKALVRVQNLSGSKLTLKTAD
GKTDVVKDVGPQSHGDREINPVKVNLALFDGSKKVSDLKPVTLARGEVVCLYVTGSGGKLAPVWVKRPVKAD
*

AnnotationID:3638 GeneID:3630

AnnotatorUID: danlee

Modification Date/Time: 2006-03-11 16:04:53

Gene Name: algF

Confidence Code: 1

GeneProduct: alginate O-acetyltransferase

Cell Localization: ()

Synonyms:

Cell Localization Confidence Code: 5

MolecularFunction:

Functional Category: (23) Secreted Factors (toxins, enzymes, alginate)

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs:

Secondary Functional Category(ies): Adaptation, protection; Cell wall / LPS / capsule

EC Number:

Status: ACTIVE

Pathway:

Homology:

>gb|AAO54758.1| (AE016860) alginate biosynthesis protein AlgF [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 222

 Score =  261 bits (667), Expect = 2e-69
 Identities = 129/200 (64%), Positives = 154/200 (77%)

>gb|AAN66902.1|AE016318_9 (AE016778) alginate O-acetyltransferase [Pseudomonas putida KT2440]
          Length = 215

 Score =  244 bits (622), Expect = 3e-64
 Identities = 128/204 (62%), Positives = 145/204 (71%), Gaps = 1/204 (0%)

Structural Features:

CDD Search: No hits above 0.01 threshold.

Genomic Context:

Comment:

ReferenceID:7263

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:24405

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

ReferenceID:34141

Author/Investigator(s): Shinabarger D, May TB, Boyd A, Ghosh M, Chakrabarty AM.

Title: Nucleotide sequence and expression of the Pseudomonas aeruginosa algF gene controlling acetylation of alginate.

PubMed: 7934909

MedLine:

Source:

Mol Microbiol. 1993 Sep;9(5):1027-35.

Reference Type: Journal

Data:

URL:	`http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7934909`

ReferenceID:34142

Author/Investigator(s): Franklin MJ, Ohman DE.

Title: Identification of algF in the alginate biosynthetic gene cluster of Pseudomonas aeruginosa which is required for alginate acetylation.

PubMed: 8394313

MedLine:

Source:

J Bacteriol. 1993 Aug;175(16):5057-65.

Reference Type: Journal

Data:

URL:	`http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8394313`

Homologs By Global Alignment

Gene ID:3630

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

5819 PA3550_tr translation of PA3550 216 99.53 99.53 0 1105.0

162266 gb|AAG06938.1|AE004776_1 (AE004776) alginate o-acetyltransferase AlgF [Pseudomonas aeruginosa PAO1] 216 99.53 99.53 0 1105.0

162267 gb|AAB09783.1| (U50202) AlgF [Pseudomonas aeruginosa] 216 99.53 99.53 0 1105.0

162268 gb|AAD15231.1| (L13026) alginate O-acetyltransferase [Pseudomonas aeruginosa] 216 99.07 99.07 0 1100.0

162269 gb|AAP46699.1| (AF527790) AlgF [Pseudomonas fluorescens] 220 60.90 73.18 7 674.0

GC ORFID: `46632`	How Found: `BLASTX`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name: `t_PA3550`	Glimmer Score:
Start: `3372986`	Stop: `3373636`
Length: `651`
Start Codon: `ATG`	Truncated Start:
Stop Codon: `TGA`	Truncated Stop:
Homolog: `t_PA3550 translation of PA3550`	Homolog Bit Score `430.0`
Other Homologs: `psyr_15may02_Scaffold3_revised_gene5001 (ORF: BLASTX 265.0), t_PA3550 (ORF: GLIMMER)`
GC ORF Sequence
ATGAACCCGATGACCCGCCGCCACACCTGGACCCGCCTGGCCTGCGCACTTTCCCTCGGCGTCGCCGCATTC GCCGCGCAGGCCGACGAGGGCGCCCTGTACGGGCCCCAGGCACCCAAGGGCTCGGCCTTCGTCCGCGCCTAC AACGCCGGCAACAGCGAACTCGACGTGTCGGTCGGCAGCACCAGCCTGAACGACGTCGCGCCGCTCGGTTCC AGCGACTTCAAGTTCCTCCCCCCCGGCAGCTACACCGCCCAGGTCGGCCAGCAGAGCCTGCAGGTCAAGCTC GACCCGGACAGCTACTACACCCTGGTCAGCCAGCCCGGCGGCAAGCCGCAGCTGGTGGCCGAGCCGCCGTTC AAGAACAAGCAGAAGGCGCTGGTACGGGTGCAGAACCTCAGCGGCTCGAAGCTGACCCTGAAGACCGCCGAC GGCAAGACCGACGTGGTCAAGGACGTCGGCCCGCAAAGCCACGGCGACCGCGAGATCAACCCGGTGAAGGTG AACCTGGCGCTGTTCGACGGCAGCAAGAAGGTCAGCGACCTGAAGCCAGTCACCCTGGCCCGCGGCGAGGTG GTCTGCCTGTACGTCACCGGCAGCGGCGGCAAGCTCGCGCCGGTCTGGGTCAAGCGCCCGGTGAAGGCGGAC TGA