ViewGene
Locus Name:
GeneID:
Location:

GeneID:362
TrivialName: PA14_43380
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_43380
PAO1 Ortholog Locus:PA1634
Sequence Length:2073
Protein Length:690
Start: 3865748
Stop: 3863676
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05023.1|AE004591_3 (AE004591) potassium-transporting ATPase, B chain [Pseudomonas aeruginosa PAO1]
Identities = 688/690 (99%), Positives = 689/690 (99%)
Sequence:
ATGAACGCTCAGACCCAGGCGTCCAAGAACGCCTTGCACAAATCGCTCGAACGTCGCCACAACGCGTCCACC
AGCCTCGCCGCCTTGTGGCGGCCGGCGCTGCTGCAAGCCTTCGTCAAGCTCGACCCGCGCCAGTTGCTGCGC
TCGCCGGTGATGCTGGTGGTCGAACTGACCGCCGTGCTCACCACCTTGCTGTGCTTCATCCCCGACCAGGCG
GTGCCGACCTCGGTGGCGCTGCAGATCGCCCTCTGGCTGTGGTTCACCGTGCTCTTCGCCAACTTCGCCGAA
GCGCTCGCCGAGGGGCGTGGCAAGGCCCGCGCGGACAGTCTCAAGGCCGGCAGCCAGGGCCTCTCCGCCAAT
AAGCGGCGCGCCGACGGCAGTTTCGAGAGCGTCGCTGCCAGCAGCCTGCGCAAGGGCGACGTGGTACGGGTC
CAGGCCGGCGAGATGATTCCCGGCGACGGCGAAGTCATCGAAGGCGTGGCGGCGGTCAACGAGGCCGCCATC
ACCGGCGAATCGGCGCCGGTGATCCGCGAGTCCGGCGGCGACCGCTCGGCGGTGACCGGCAACACCCAGGTG
GTGTCCGACTGGCTGCTGGTACGGATCGGCGCCAACCCGGGCGAGTCGACCCTGGACCGGATGATCGCCCTG
GTGGAAGGCGCCAAGCGGCAGAAGACCCCCAACGAAGTAGCGCTGGACATCCTGCTGATCGGCCTGACCCTG
ATCTTCCTGCTGGTGGTGGTGACCCTCAAGCCGTTCGCCCTGTTCGCCGGCGGCAACCTGCCGCTGGTGTTC
CTGGTGGCGCTGCTGGTGACGCTGATCCCGACCACCATCGGCGGCCTGCTCTCGGCCATCGGCATCGCCGGT
ATGGACCGCCTGGTGCGCCTGAACGTGATCGCCAAGTCCGGTCGCGCGGTGGAGGCGGCCGGCGACGTCCAC
GTGCTGCTGCTCGACAAGACCGGCACCATCACCTTCGGCAACCGCCGCTGCAGCGCGCTGTACGCCGCGCCG
GGGGTGAGCGGCAAGGAACTGGCCGAGGGCGCGTTGCTGGCCTCGCTGGCCGATGACACGCCGGAAGGCAAG
TCGATCGTCGAATACCTGCGCATCCTGCATCCGATCGAGGAGCCGCGCCGCGAGGAGCTGACGCCGATCGCC
TTCAGCGCGGAAACCCGCCTGTCCGGGGTCGACTGGAACGGCCAGGTGTACCGCAAGGGGGCGGTGGATGCG
GCGCTGCGCTTCATCGACCTGCCGCGGGAGAAGATGCCCGAGGTGCTTTCGCGGGAGGTGGACAAGATCGCC
CAGAGCGGCGGCACCCCGCTGCTGGTGGTAGCCAACGGCCGGCTGCTCGGCGCCATTCACCTGAAGGACGTG
GTCAAGCCGGGCATTCGCGAGCGCTTCGCCGAACTGCGCCGGCTGGGCATCCGCACTGTGATGGTGACCGGC
GACAACCCGCTGACCGCCGCTGCCATCGCCGCCGAGGCTGGGGTCGACGACGTGATCGCCGAGGCTACCCCG
GAGAAGAAACTGGCGCGGATTCGCCAGGAGCAGGGCGAAGGTCGCCTGGTGGCGATGTGCGGCGACGGTGCC
AACGACGCCCCGGCGCTGGCCCAGGCCGACGTCGGCATGGCCATGAACGACGGCACCCAGGCCGCCCGCGAG
GCGGCCAACCTGGTCGACCTGGACAGCGACCCGACCAAGCTGCTCGACGTGGTCCAGGTGGGCAAGGAGTTG
CTGGTCACCCGCGGCGCGCTGACCACCTTCTCCATCGCCAACGACGTGGCCAAGTACTTCGCCATCCTGCCG
GCGCTGTTCGTCGGCATCTACCCGCAGCTCGACGTGCTCAACGTGATGCGCCTGCAAAGCCCGCAGAGCGCG
ATCCTGTCGGCCATCGTGTTCAACGCGCTGATCATCGTCGCGTTGATCCCACTGGCCTTGCGCGGCGTGCGG
GTGCAGGCGGCGGACGCTTCGCAACTGCTGCAGCGCAACCTGCTGATCTATGGCGTCGGCGGCCTGGTGGCG
CCGTTCCTCGGGATCAAGGCGATCGACCTGCTGCTGACCGCCGTCGGCCTGGCATGA
Translation:
MNAQTQASKNALHKSLERRHNASTSLAALWRPALLQAFVKLDPRQLLRSPVMLVVELTAVLTTLLCFIPDQA
VPTSVALQIALWLWFTVLFANFAEALAEGRGKARADSLKAGSQGLSANKRRADGSFESVAASSLRKGDVVRV
QAGEMIPGDGEVIEGVAAVNEAAITGESAPVIRESGGDRSAVTGNTQVVSDWLLVRIGANPGESTLDRMIAL
VEGAKRQKTPNEVALDILLIGLTLIFLLVVVTLKPFALFAGGNLPLVFLVALLVTLIPTTIGGLLSAIGIAG
MDRLVRLNVIAKSGRAVEAAGDVHVLLLDKTGTITFGNRRCSALYAAPGVSGKELAEGALLASLADDTPEGK
SIVEYLRILHPIEEPRREELTPIAFSAETRLSGVDWNGQVYRKGAVDAALRFIDLPREKMPEVLSREVDKIA
QSGGTPLLVVANGRLLGAIHLKDVVKPGIRERFAELRRLGIRTVMVTGDNPLTAAAIAAEAGVDDVIAEATP
EKKLARIRQEQGEGRLVAMCGDGANDAPALAQADVGMAMNDGTQAAREAANLVDLDSDPTKLLDVVQVGKEL
LVTRGALTTFSIANDVAKYFAILPALFVGIYPQLDVLNVMRLQSPQSAILSAIVFNALIIVALIPLALRGVR
VQAADASQLLQRNLLIYGVGGLVAPFLGIKAIDLLLTAVGLA*
AnnotationID:371GeneID:362
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 07:07:09
Gene Name:kdpB
Confidence Code:2
GeneProduct:potassium-transporting ATPase, B chain
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2216,COG0474,pfam00122
Secondary Functional Category(ies):
EC Number:3.6.3.12
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55759.1| (AE016863) potassium-transporting ATPase, B subunit [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 692

 Score = 1098 bits (2841), Expect = 0.0
 Identities = 571/688 (82%), Positives = 626/688 (90%), Gaps = 3/688 (0%)

>gb|AAL44599.1| (AE009310) potassium-transporting ATPase B chain [Agrobacterium
           tumefaciens str. C58 (U. Washington)]
          Length = 694

 Score =  815 bits (2106), Expect = 0.0
 Identities = 438/679 (64%), Positives = 531/679 (78%), Gaps = 21/679 (3%)

>dbj|BAB34148.1| (AP002552) high-affinity potassium-transporting ATPase B chain
           [Escherichia coli O157:H7]
          Length = 682

 Score =  754 bits (1947), Expect = 0.0
 Identities = 403/665 (60%), Positives = 510/665 (76%), Gaps = 8/665 (1%)








 
Structural Features:
COG2216, KdpB, High-affinity K+ transport system, ATPase chain B 
[Inorganic ion transport and metabolism]
CD-Length = 681 residues, 100.0% aligned
Score = 1009 bits (2610), Expect = 0.0
Identities = 458/681 (67%), Positives = 541/681 (79%), Gaps = 8/681 (1%)

COG0474, MgtA, Cation transport ATPase 
[Inorganic ion transport and metabolism]
CD-Length = 917 residues,  73.6% aligned
Score =  215 bits (548), Expect = 1e-56
Identities = 169/700 (24%), Positives = 284/700 (40%), Gaps = 127/700 (18%)

pfam00122, E1-E2_ATPase, E1-E2 ATPase.
CD-Length = 223 residues, 100.0% aligned
Score =  146 bits (369), Expect = 1e-35
Identities = 72/228 (31%), Positives = 110/228 (48%), Gaps = 9/228 (3%)









   
Genomic Context:






   
Comment:


















    ReferenceID:727




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17869




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37260




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:362_cdd.html


    		




 
URL:

















    ReferenceID:37886




  
Author/Investigator(s):
        Treuner-Lange A, Kuhn A, Durre P.





  
Title:
        The kdp system of Clostridium acetobutylicum: cloning, sequencing, and transcriptional regulation in response to potassium concentration.




  
PubMed:
        9226259


MedLine:
        




    
Source:
J Bacteriol. 1997 Jul;179(14):4501-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9226259

















    ReferenceID:37889




  
Author/Investigator(s):
        Sugiura A, Nakashima K, Tanaka K, Mizuno T.





  
Title:
        Clarification of the structural and functional features of the osmoregulated kdp operon of Escherichia coli.




  
PubMed:
        1630316


MedLine:
        




    
Source:
Mol Microbiol. 1992 Jul;6(13):1769-76.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1630316

















    ReferenceID:37897




  
Author/Investigator(s):
        Gassel M, Siebers A, Epstein W, Altendorf K.





  
Title:
        Assembly of the Kdp complex, the multi-subunit K+-transport ATPase of Escherichia coli.




  
PubMed:
        9858692


MedLine:
        




    
Source:
Biochim Biophys Acta. 1998 Dec 9;1415(1):77-84.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=99077600&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:362
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
606
                           PA1634_tr
                           translation of PA1634
                           690
                           99.71
                           99.85
                           0
                           3374.0
     

     
     
28730
                           gb|AAG05023.1|AE004591_3
                           (AE004591) potassium-transporting ATPase, B chain [Pseudomonas aeruginosa PAO1]
                           690
                           99.71
                           99.85
                           0
                           3374.0
     

     
     
28731
                           gb|AAO55759.1|
                           (AE016863) potassium-transporting ATPase, B subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           693
                           82.39
                           90.47
                           4
                           2842.0
     

     
     
28734
                           gb|AAK65912.1|
                           (AE007310) probable KdpB potassium-transporting ATPase B chain [Sinorhizobium meliloti]
                           703
                           63.86
                           74.82
                           36
                           2106.0
     

     
     
28732
                           gb|AAL44599.1|
                           (AE009310) potassium-transporting ATPase B chain [Agrobacterium tumefaciens str. C58 (U. Washington)]
                           710
                           61.97
                           75.63
                           36
                           2124.0
     

     






      

          
GC ORFID: 43364How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1634Glimmer Score: 

          
Start: 1088161Stop: 1090233

          
Length: 2073


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1634 translation of PA1634Homolog Bit Score 1304.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1035 (ORF: BLASTX 1108.0), t_PA1634 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGCTCAGACCCAGGCGTCCAAGAACGCCTTGCACAAATCGCTCGAACGTCGCCACAACGCGTCCACC 
AGCCTCGCCGCCTTGTGGCGGCCGGCGCTGCTGCAAGCCTTCGTCAAGCTCGACCCGCGCCAGTTGCTGCGC 
TCGCCGGTGATGCTGGTGGTCGAACTGACCGCCGTGCTCACCACCTTGCTGTGCTTCATCCCCGACCAGGCG 
GTGCCGACCTCGGTGGCGCTGCAGATCGCCCTCTGGCTGTGGTTCACCGTGCTCTTCGCCAACTTCGCCGAA 
GCGCTCGCCGAGGGGCGTGGCAAGGCCCGCGCGGACAGTCTCAAGGCCGGCAGCCAGGGCCTCTCCGCCAAT 
AAGCGGCGCGCCGACGGCAGTTTCGAGAGCGTCGCTGCCAGCAGCCTGCGCAAGGGCGACGTGGTACGGGTC 
CAGGCCGGCGAGATGATTCCCGGCGACGGCGAAGTCATCGAAGGCGTGGCGGCGGTCAACGAGGCCGCCATC 
ACCGGCGAATCGGCGCCGGTGATCCGCGAGTCCGGCGGCGACCGCTCGGCGGTGACCGGCAACACCCAGGTG 
GTGTCCGACTGGCTGCTGGTACGGATCGGCGCCAACCCGGGCGAGTCGACCCTGGACCGGATGATCGCCCTG 
GTGGAAGGCGCCAAGCGGCAGAAGACCCCCAACGAAGTAGCGCTGGACATCCTGCTGATCGGCCTGACCCTG 
ATCTTCCTGCTGGTGGTGGTGACCCTCAAGCCGTTCGCCCTGTTCGCCGGCGGCAACCTGCCGCTGGTGTTC 
CTGGTGGCGCTGCTGGTGACGCTGATCCCGACCACCATCGGCGGCCTGCTCTCGGCCATCGGCATCGCCGGT 
ATGGACCGCCTGGTGCGCCTGAACGTGATCGCCAAGTCCGGTCGCGCGGTGGAGGCGGCCGGCGACGTCCAC 
GTGCTGCTGCTCGACAAGACCGGCACCATCACCTTCGGCAACCGCCGCTGCAGCGCGCTGTACGCCGCGCCG 
GGGGTGAGCGGCAAGGAACTGGCCGAGGGCGCGTTGCTGGCCTCGCTGGCCGATGACACGCCGGAAGGCAAG 
TCGATCGTCGAATACCTGCGCATCCTGCATCCGATCGAGGAGCCGCGCCGCGAGGAGCTGACGCCGATCGCC 
TTCAGCGCGGAAACCCGCCTGTCCGGGGTCGACTGGAACGGCCAGGTGTACCGCAAGGGGGCGGTGGATGCG 
GCGCTGCGCTTCATCGACCTGCCGCGGGAGAAGATGCCCGAGGTGCTTTCGCGGGAGGTGGACAAGATCGCC 
CAGAGCGGCGGCACCCCGCTGCTGGTGGTAGCCAACGGCCGGCTGCTCGGCGCCATTCACCTGAAGGACGTG 
GTCAAGCCGGGCATTCGCGAGCGCTTCGCCGAACTGCGCCGGCTGGGCATCCGCACTGTGATGGTGACCGGC 
GACAACCCGCTGACCGCCGCTGCCATCGCCGCCGAGGCTGGGGTCGACGACGTGATCGCCGAGGCTACCCCG 
GAGAAGAAACTGGCGCGGATTCGCCAGGAGCAGGGCGAAGGTCGCCTGGTGGCGATGTGCGGCGACGGTGCC 
AACGACGCCCCGGCGCTGGCCCAGGCCGACGTCGGCATGGCCATGAACGACGGCACCCAGGCCGCCCGCGAG 
GCGGCCAACCTGGTCGACCTGGACAGCGACCCGACCAAGCTGCTCGACGTGGTCCAGGTGGGCAAGGAGTTG 
CTGGTCACCCGCGGCGCGCTGACCACCTTCTCCATCGCCAACGACGTGGCCAAGTACTTCGCCATCCTGCCG 
GCGCTGTTCGTCGGCATCTACCCGCAGCTCGACGTGCTCAACGTGATGCGCCTGCAAAGCCCGCAGAGCGCG 
ATCCTGTCGGCCATCGTGTTCAACGCGCTGATCATCGTCGCGTTGATCCCACTGGCCTTGCGCGGCGTGCGG 
GTGCAGGCGGCGGACGCTTCGCAACTGCTGCAGCGCAACCTGCTGATCTATGGCGTCGGCGGCCTGGTGGCG 
CCGTTCCTCGGGATCAAGGCGATCGACCTGCTGCTGACCGCCGTCGGCCTGGCATGA