ViewGene
Locus Name:
GeneID:
Location:

GeneID:3617
TrivialName: PA14_38550
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_38550
PAO1 Ortholog Locus:PA2007
Sequence Length:639
Protein Length:212
Start: 3438067
Stop: 3438705
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05395.1|AE004627_3 (AE004627) maleylacetoacetate isomerase [Pseudomonas aeruginosa PAO1]
Identities = 209/212 (98%), Positives = 210/212 (99%)
Sequence:
ATGAAGCTCTATACCTACTACCGTTCCACCTCCTCCTACCGGGTGCGCATCGCCCTGGCGCTGAAGGGGCTG
GACTACCAGTCGCTGCCGGTCAACCTGGTCCGCGACGGCGGCGAACACCGCCAACCCGCCTACCTGGCGCTC
AACCCGCAGGGCCGCGTACCGGCCCTGCAGGTCGACGAGGGCGAGCTGCTGATCCAGTCGCCGGCGATCATC
GAGTACCTCGAGGAGCGCTACCCGCACCCCGCCCTGCTCTCCAGCGATCCGCTGCGCCGCGCCCACGAGCGC
GGAGTCGCGGCGCTGGTCGGCTGCGACATCCACCCGTTGCACAACGCCAGCGTGCTCAACCTGCTGCGCCAG
TGGGGACACGACGAAGAGCAGGTCCGGCAGTGGATCGGCCACTGGGTCGGCCAGGGTCTCGCCGCCGTCGAG
CAACTGATCGGCGACCAGGGTTGGTGCTTCGGCGACCGGCCAGGCCTGGCCGACGTCTACCTGGTCCCGCAG
CTCTACGCCGCCGAGCGCTTCGGCGTGGCGCTGGACGCCTGGCCACGGATCCGCCGGGTCGCCGACCTGGCC
GCCGCCCATCCGGCGTTCCGCCAGGCCCATCCGGCCAACCAGCCGGACACCCCGGCCGCCTGA
Translation:
MKLYTYYRSTSSYRVRIALALKGLDYQSLPVNLVRDGGEHRQPAYLALNPQGRVPALQVDEGELLIQSPAII
EYLEERYPHPALLSSDPLRRAHERGVAALVGCDIHPLHNASVLNLLRQWGHDEEQVRQWIGHWVGQGLAAVE
QLIGDQGWCFGDRPGLADVYLVPQLYAAERFGVALDAWPRIRRVADLAAAHPAFRQAHPANQPDTPAA*
AnnotationID:3625GeneID:3617
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 17:05:47
Gene Name:maiA
Confidence Code:2
GeneProduct:maleylacetoacetate isomerase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:glutathione-S-transferase
Functional Category Confidence Code:5
COGs:COG0625
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:5.2.1.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70192.1|AE016658_9 (AE016791) maleylacetoacetate isomerase, putative [Pseudomonas
           putida KT2440]
          Length = 210

 Score =  322 bits (824), Expect = 1e-87
 Identities = 159/211 (75%), Positives = 178/211 (84%), Gaps = 2/211 (0%)

>dbj|BAC45374.1| (AP005935) maleylacetoacetate isomerase [Bradyrhizobium japonicum
           USDA 110]
          Length = 210

 Score =  198 bits (503), Expect = 2e-50
 Identities = 112/211 (53%), Positives = 141/211 (66%), Gaps = 6/211 (2%)

>emb|CAE41534.1| (BX640414) putative glutathione-S-transferase [Bordetella
           pertussis]
          Length = 214

 Score =  192 bits (489), Expect = 9e-49
 Identities = 108/213 (50%), Positives = 133/213 (62%), Gaps = 6/213 (2%)






 
Structural Features:
COG0625, Gst, Glutathione S-transferase 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 211 residues, 100.0% aligned
Score =  139 bits (350), Expect = 3e-34
Identities = 79/214 (36%), Positives = 108/214 (50%), Gaps = 6/214 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7237




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24379




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32721




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3617_cdd.html


    		




 
URL:

















    ReferenceID:34380




  
Author/Investigator(s):
        Hatakeyama K, Asai Y, Uchida Y, Kobayashi M, Terasawa M, Yukawa H.





  
Title:
        Gene cloning and characterization of maleate cis-trans isomerase from Alcaligenes faecalis.




  
PubMed:
        9345272


MedLine:
        




    
Source:
Biochem Biophys Res Commun. 1997 Oct 9;239(1):74-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9345272

















    ReferenceID:34381




  
Author/Investigator(s):
        Hatakeyama K, Goto M, Uchida Y, Kobayashi M, Terasawa M, Yukawa H.





  
Title:
        Molecular analysis of maleate cis-trans isomerase from thermophilic bacteria.




  
PubMed:
        10803955


MedLine:
        




    
Source:
Biosci Biotechnol Biochem. 2000 Mar;64(3):569-76.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10803955









           
Homologs By Global Alignment 

           

       



     
Gene ID:3617
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5802
                           PA2007_tr
                           translation of PA2007
                           212
                           98.58
                           99.05
                           0
                           1111.0
     

     
     
161910
                           gb|AAG05395.1|AE004627_3
                           (AE004627) maleylacetoacetate isomerase [Pseudomonas aeruginosa PAO1]
                           212
                           98.58
                           99.05
                           0
                           1111.0
     

     
     
161911
                           gb|AAN70192.1|AE016658_9
                           (AE016791) maleylacetoacetate isomerase, putative [Pseudomonas putida KT2440]
                           212
                           75.00
                           83.96
                           2
                           825.0
     

     
     
161912
                           gb|AAO57029.1|
                           (AE016868) maleylacetoacetate isomerase [Pseudomonas syringae pv. tomato str. DC3000]
                           212
                           66.03
                           79.71
                           1
                           752.0
     

     
     
161913
                           dbj|BAC45374.1|
                           (AP005935) maleylacetoacetate isomerase [Bradyrhizobium japonicum USDA 110]
                           215
                           52.09
                           65.58
                           8
                           511.0
     

     






      

          
GC ORFID: 46619How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2007Glimmer Score: 

          
Start: 1515844Stop: 1515206

          
Length: 639


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2007 translation of PA2007Homolog Bit Score 432.0

          
Other Homologs:  t_PA2473 (ORF: BLASTX 164.0), t_PA2007 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGCTCTATACCTACTACCGTTCCACCTCCTCCTACCGGGTGCGCATCGCCCTGGCGCTGAAGGGGCTG 
GACTACCAGTCGCTGCCGGTCAACCTGGTCCGCGACGGCGGCGAACACCGCCAACCCGCCTACCTGGCGCTC 
AACCCGCAGGGCCGCGTACCGGCCCTGCAGGTCGACGAGGGCGAGCTGCTGATCCAGTCGCCGGCGATCATC 
GAGTACCTCGAGGAGCGCTACCCGCACCCCGCCCTGCTCTCCAGCGATCCGCTGCGCCGCGCCCACGAGCGC 
GGAGTCGCGGCGCTGGTCGGCTGCGACATCCACCCGTTGCACAACGCCAGCGTGCTCAACCTGCTGCGCCAG 
TGGGGACACGACGAAGAGCAGGTCCGGCAGTGGATCGGCCACTGGGTCGGCCAGGGTCTCGCCGCCGTCGAG 
CAACTGATCGGCGACCAGGGTTGGTGCTTCGGCGACCGGCCAGGCCTGGCCGACGTCTACCTGGTCCCGCAG 
CTCTACGCCGCCGAGCGCTTCGGCGTGGCGCTGGACGCCTGGCCACGGATCCGCCGGGTCGCCGACCTGGCC 
GCCGCCCATCCGGCGTTCCGCCAGGCCCATCCGGCCAACCAGCCGGACACCCCGGCCGCCTGA