ViewGene
Locus Name:
GeneID:
Location:

GeneID:3611
TrivialName: PA14_63150
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_63150
PAO1 Ortholog Locus:PA4776
Sequence Length:666
Protein Length:221
Start: 5635995
Stop: 5636660
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08162.1|AE004891_4 (AE004891) probable two-component response regulator [Pseudomonas aeruginosa PAO1]
Identities = 221/221 (100%), Positives = 221/221 (100%)
Sequence:
ATGAGAATACTGCTGGCCGAGGACGACCTGCTGCTCGGCGACGGCATCCGCGCCGGGCTGCGCCTGGAAGGC
GATACCGTGGAATGGGTGACCGACGGCGTGGCCGCGGAGAACGCGCTGGTCACCGACGAGTTCGACCTGCTG
GTGCTCGACATCGGCCTGCCGCGCCGCAGCGGCCTGGACATCCTGCGCAACCTGCGTCACCAGGGCCTGCTC
ACCCCGGTGCTGCTGCTCACCGCGCGGGACAAGGTGGCCGACCGGGTCGCCGGGCTCGACAGCGGTGCCGAC
GACTACCTGACCAAGCCCTTCGATCTCGACGAACTGCAGGCACGGGTGCGCGCCCTGACCCGCCGCACCACC
GGTCGCGCCCTGCCGCAACTGGTGCACGGCGAGCTGCGCCTGGACCCGGCGACCCACCAGGTGACCCTGTCC
GGCCAGGCGGTGGAACTGGCGCCGCGCGAATACGCGCTGCTGCGCCTGCTGCTGGAGAACAGCGGCAAGGTG
CTCTCGCGCAACCAACTGGAGCAGAGCCTCTACGGCTGGAGCGGCGACGTCGAGAGCAACGCCATCGAGGTC
CATGTCCATCACCTGCGGCGCAAGCTCGGCAACCAGTTGATCCGCACCGTCCGCGGCATCGGCTACGGCATC
GACCAGCCGGCGCCCTGA
Translation:
MRILLAEDDLLLGDGIRAGLRLEGDTVEWVTDGVAAENALVTDEFDLLVLDIGLPRRSGLDILRNLRHQGLL
TPVLLLTARDKVADRVAGLDSGADDYLTKPFDLDELQARVRALTRRTTGRALPQLVHGELRLDPATHQVTLS
GQAVELAPREYALLRLLLENSGKVLSRNQLEQSLYGWSGDVESNAIEVHVHHLRRKLGNQLIRTVRGIGYGI
DQPAP*
AnnotationID:3619GeneID:3611
AnnotatorUID: diggins
Modification Date/Time: 2005-03-09 12:12:42
Gene Name:pmrA
Confidence Code:1
GeneProduct:two-component response regulator
Cell Localization:()
Synonyms:ygiX
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:DNA-binding response regulator
Functional Category Confidence Code:5
COGs:COG0745,cd00156
Secondary Functional Category(ies):antibiotic resistance and susceptibility
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO90736.1| (AE016963) DNA-binding response regulator [Coxiella burnetii RSA
           493]
          Length = 223

 Score =  263 bits (673), Expect = 4e-70
 Identities = 124/220 (56%), Positives = 176/220 (80%), Gaps = 2/220 (0%)

>gb|AAO55412.1| (AE016862) DNA-binding response regulator [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 220

 Score =  248 bits (634), Expect = 1e-65
 Identities = 123/220 (55%), Positives = 161/220 (73%)

>gb|AAO70642.1| (AE016844) probable two-component system response regulator.
           [Salmonella enterica subsp. enterica serovar Typhi Ty2]
          Length = 219

 Score =  235 bits (600), Expect = 1e-61
 Identities = 118/214 (55%), Positives = 152/214 (71%)






 
Structural Features:
COG0745, OmpR, Response regulators consisting of a CheY-like receiver domain 
and a winged-helix DNA-binding domain 
[Signal transduction mechanisms / Transcription]
CD-Length = 229 residues,  97.4% aligned
Score =  205 bits (522), Expect = 5e-54
Identities = 103/224 (45%), Positives = 144/224 (64%), Gaps = 11/224 (4%)

cd00156, REC, Signal receiver domain
CD-Length = 113 residues,  99.1% aligned
Score =  102 bits (256), Expect = 3e-23
Identities = 49/112 (43%), Positives = 68/112 (60%)






   
Genomic Context:






   
Comment:


















    ReferenceID:7225




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:24367




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37684




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:3611_cdd.html


    		




 
URL:

















    ReferenceID:37705




  
Author/Investigator(s):
        Moskowitz SM, Ernst RK, Miller SI.





  
Title:
        PmrAB, a two-component regulatory system of Pseudomonas aeruginosa that modulates resistance to cationic antimicrobial peptides and addition of aminoarabinose to lipid A.




  
PubMed:
        14702327


MedLine:
        




    
Source:
J Bacteriol. 2004 Jan;186(2):575-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=14702327

















    ReferenceID:37706




  
Author/Investigator(s):
        McPhee JB, Lewenza S, Hancock RE.





  
Title:
        Cationic antimicrobial peptides activate a two-component regulatory system, PmrA-PmrB, that regulates resistance to polymyxin B and cationic antimicrobial peptides in Pseudomonas aeruginosa.




  
PubMed:
        14507375


MedLine:
        




    
Source:
Mol Microbiol. 2003 Oct;50(1):205-17.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14507375









           
Homologs By Global Alignment 

           

       



     
Gene ID:3611
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
5782
                           PA4776_tr
                           translation of PA4776
                           221
                           99.99
                           99.99
                           0
                           1113.0
     

     
     
161297
                           gb|AAG08162.1|AE004891_4
                           (AE004891) probable two-component response regulator [Pseudomonas aeruginosa PAO1]
                           221
                           99.99
                           99.99
                           0
                           1113.0
     

     
     
161300
                           gb|AAN68321.1|AE016466_6
                           (AE016784) DNA-binding response regulator [Pseudomonas putida KT2440]
                           221
                           56.56
                           72.85
                           1
                           627.0
     

     
     
161301
                           gb|AAQ58565.1|
                           (AE016913) Transcriptional regulatory protein qseB [Chromobacterium violaceum ATCC 12472]
                           221
                           55.65
                           68.77
                           1
                           608.0
     

     
     
161299
                           gb|AAO55412.1|
                           (AE016862) DNA-binding response regulator [Pseudomonas syringae pv. tomato str. DC3000]
                           221
                           55.65
                           72.85
                           1
                           634.0
     

     






      

          
GC ORFID: 46613How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4776Glimmer Score: 

          
Start: 4899675Stop: 4900340

          
Length: 666


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4776 translation of PA4776Homolog Bit Score 433.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7577 (ORF: BLASTX 253.0), t_PA2479 (ORF: BLASTX 230.0), t_PA2657 (ORF: BLASTX 186.0), psyr_15may02_Scaffold17_revised_gene2789 (ORF: BLASTX 182.0), t_PA0756 (ORF: BLASTX 174.0), psyr_15may02_Scaffold16_revised_gene2699 (ORF: BLASTX 172.0), t_PA2809 (ORF: BLASTX 162.0), psyr_15may02_Scaffold10_revised_gene1701 (ORF: BLASTX 158.0), psyr_15may02_Scaffold9_revised_gene8194 (ORF: BLASTX 149.0), t_PA1179 (ORF: BLASTX 145.0), psyr_15may02_Scaffold12_revised_gene2057 (ORF: BLASTX 141.0), t_PA4776 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGAATACTGCTGGCCGAGGACGACCTGCTGCTCGGCGACGGCATCCGCGCCGGGCTGCGCCTGGAAGGC 
GATACCGTGGAATGGGTGACCGACGGCGTGGCCGCGGAGAACGCGCTGGTCACCGACGAGTTCGACCTGCTG 
GTGCTCGACATCGGCCTGCCGCGCCGCAGCGGCCTGGACATCCTGCGCAACCTGCGTCACCAGGGCCTGCTC 
ACCCCGGTGCTGCTGCTCACCGCGCGGGACAAGGTGGCCGACCGGGTCGCCGGGCTCGACAGCGGTGCCGAC 
GACTACCTGACCAAGCCCTTCGATCTCGACGAACTGCAGGCACGGGTGCGCGCCCTGACCCGCCGCACCACC 
GGTCGCGCCCTGCCGCAACTGGTGCACGGCGAGCTGCGCCTGGACCCGGCGACCCACCAGGTGACCCTGTCC 
GGCCAGGCGGTGGAACTGGCGCCGCGCGAATACGCGCTGCTGCGCCTGCTGCTGGAGAACAGCGGCAAGGTG 
CTCTCGCGCAACCAACTGGAGCAGAGCCTCTACGGCTGGAGCGGCGACGTCGAGAGCAACGCCATCGAGGTC 
CATGTCCATCACCTGCGGCGCAAGCTCGGCAACCAGTTGATCCGCACCGTCCGCGGCATCGGCTACGGCATC 
GACCAGCCGGCGCCCTGA